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Abdul Jaleel K. A, PhD

Scientist E-II

+91-471-2529540

jaleel@rgcb.res.in

abduljaleel
abduljaleel

Abdul Jaleel K. A, PhD

Scientist E-II

+91-471-2529540

jaleel@rgcb.res.in

  • Profile

    • Post Doctoral 2001-2006 (Mayo Clinic, Rochester, Minnesota, USA)
    • Ph.D 1998 (All India Institute of Medical Sciences / Jamia Millia Islamia, New Delhi)
    • M.Sc Medical Microbiology (Mahatma Gandhi University, Kottayam)
    • Dec 2011, Present: Scientist - E-II, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram - 14, Kerala
    • 2010 - 2011: Director of Laboratories & Research, Cellaram Diabetes Institute, Pune, India
    • 2007- 2010: Assistant Professor of Medicine, Mayo Clinic, Rochester, Minnesota, USA
    • 2007: Novel Methodology Development Award (NMDA) from Mayo Clinic, Center for Translational Science Activities (CTSA)
    • 2003: NIH Travel Grant for the meeting "Proteomics in Diabetes" NIDDK, NIH, Maryland
    • 1995: Senior Research Fellowship (UGC), India
    • 1993: Junior Research Fellowship (UGC), India
  • Research

    Metabolic transition processes associated with the onset of type 2 diabetes

    My research interest is in studying the mechanism of pathophysiology of type 2 diabetes. One area we want to focus is how independent risk factors for diabetes evolve over time in developing insulin resistance, which is the hallmark of type 2 diabetes. Type 2 diabetes is the most commonly diagnosed metabolic disease and metabolic alterations are often present years before the overt disease. Emerging technologies such as mass spectrometry have augmented the feasibility of acquiring high-throughput profiles of a whole organism's metabolic status (metabolite profiling, or metabolomics). Metabolomics allow assessment of large numbers of metabolites that are substrates, products and by-products of biochemical pathways of body metabolism. In addition to serving as potential biomarkers of disease, metabolites may have unexpected roles as regulatory signals with hormone-like functions or effectors of the pathogenesis of disease itself. At present we are developing mass spectrometry based tools of metabolomics in our laboratory. The present activity intend to identify the altered metabolic pathways in healthy people who are at risk of developing T2D (e.g., family history of T2D) by mass spectrometry based metabolomics. Towards this objective a project "Metabolic Profiling of Normal Healthy people in Kerala" is being executed by recruiting normal healthy people, but having various risk factors of T2D. We conduct human studies and besides mass spectrometry, we also employ various clinical, biochemical and hormonal measurements in the basal as well as postprandial blood of the study subjects.

    Regulation of Molecular Mechanism Underlying Adipogenesis through its mi-RNA Network

    We also have interest in investigating the molecular mechanism of adipogenesis as obesity and diabetes are highly associated due to interwoven molecular links. Adipogenesis in mammals is regulated genetically and hormonally. Adipogenic transcription factors, which regulate the expression of many adipogenic genes leading to the differentiation of adipocytes have been identified, such as peroxisome proliferator-activated receptor-gamma (PPAR-γ). PPAR- γ is the master regulator of adipogenesis and no factors have been discovered that promotes adipogenesis in the absence of PPAR-γ. So, inhibiting the activity of PPAR-γ can result in overall imbalance of body metabolism, energy balance and homeostasis. Recent research on adipocyte differentiation and maturation process has led to explain the role of micro RNA (miRNA) in controlling the whole process. The multi-functionality of PPAR- γ is supposed to be mediated through epigenetic mechanisms such as miRNA based modulation. In this contest, our lab is presently conducting studies on the modulation of upstream/downstream regulators of PPAR gamma using miRNA as molecular switches to reduce adipocyte hypertrophy and hyperplasia. Preliminary results so far carried out include the investigation of change in expression of PPAR γ, CEBPα, PGC1α, TNFα, FABP4 and Glut4 at different stages of adipocyte differentiation and compared with undifferentiated pre-adipocytes. Expression pattern of these genes in presence of PPAR gamma agonist (rosiglitazone), partial agonist (FMOC-L-Leucine) and antagonist (GW9662) were also checked in the initial phase of study.

  • Publications

    Publications

    1. Kannan M, V. Aathmanathan S, Saravanakumar M, Jaleel A, Romanelli D, Tettamanti G, Krishnan M Proteomic analysis of the silkworm midgut during larva-pupa transition. Invertebrate Survival Journal 2016; 13: 191-204
    2. Mahadevan C, Krishnan A, Saraswathy GG, Surendran A, Jaleel A, Sakuntala M. Transcriptome- assisted label-free quantitativeproteomics analysis reveals novel insights into Piper nigrum – Phytophthora capsici phytopathosystem. Frontiers in Plant Science 2016;7:785
    3. Saritha VN, George J K, Jaleel A, Surendran A, Saravanakumar M, Kalavathy MC, Somanathan T, Rema P, Sujathan K. Analysis of differentially expressed proteins in the exfoliated cells of normal and squamous cell carcinoma of the uterine cervix to define candidate markers for cervical cancer. International Journal of Biochemistry and Biotechnology 2016; 5(1):626-36
    4. Muhamed J, Rajan A, Surendran A, Jaleel A, Anilkumar TV. Comparative profiling of extractable proteins in extracellular matrices of porcine cholecyst and jejunum intended for preparation of tissue engineering scaffolds. J Biomed Mater Res B Appl Biomater.  2015, doi: 10.1002/jbm.b.33567.
    5. Gopinath V, Raghunandanan A, Gomez RL, Jose L, Surendran A, Ramachandran R, Pushparajan AR, Mundayoor S, Jaleel A and Kumar RA. Profiling the proteome of Mycobacterium tuberculosis during dormancy and reactivation. Molecular & Cellular Proteomics. 2015;14(8):2160-76
    6. Mahadevan C, Jaleel A, Deb L, Thomas G, Sakuntala M. Development of an Efficient Virus Induced Gene Silencing Strategy in the Non-Model Wild Ginger-Zingiber zerumbet and Investigation of Associated Proteome Changes. PLoS One. 2015;10(4):e0124518
    7. Abraham R, Mudaliar P, Jaleel A, Srikanth J, Sreekumar E. High throughput proteomic analysis and a comparative review identify the nuclear chaperone, Nucleophosmin among the common set of proteins modulated in Chikungunya virus infection. J Proteomics. 2015;120:126-41
    8. Dharmaprakash A, Mutt E, Jaleel E, Ramanathan S and Thomas S. Proteome profile of a pandemic Vibrio parahaemolyticus SC192 strain in the planktonic and biofilm condition. Biofouling 2014;30(6):729-39
    9. Ammu Mathew , Ganapati Natarajan , Lauri Lehtovaara , Hannu Häkkinen, Ravva Mahesh Kumar, Venkatesan Subramanian, Abdul Jaleel,  Thalappil Pradeep. Supramolecular Functionalization and Concomitant Enhancement in Properties of Au25 Clusters. ACS Nano  2014;8(1):139-52
    10. Piotr Zabielski, G. Charles Ford, X. Mai Persson, Abdul Jaleel, Jerry D. Dewey and K. Sreekumaran Nair. Comparison of different mass spectrometry techniques in the measurement of L-[ring-13C6] phenylalanine incorporation into mixed muscle proteins.  Journal of Mass Spectrometry 48(2):269-75, 2013
    11. Abdul Jaleel, Gregory C. Henderson, Benjamin J. Madden, Katherine A. Klaus, Dawn M.  Morse,  Srinivas  Gopala,  and  K.  Sreekumaran  Nair.  Nair.  Identification  of  De  Novo Synthesized  and  Relatively  Older  Proteins  Accelerated  Oxidative  Damage  to  De  Novo Synthesized Apolipoprotein A-1 in Type 1 Diabetes.  Diabetes 59:2366–2374, 2010
    12. Abdul Jaleel, Klaus K, Morse D, Karakelides H, Irving BA, K. Nair KS. Differential Effects of Insulin Deprivation and Systemic Insulin Treatment on Plasma Protein Synthesis in Type 1 Diabetic People. Am J Physiol Endocrinol Metab 297: E889–E897, 2009
    13. Abdul Jaleel, Marion Jourdan, , Helen Karakelides, G. Charles Ford Barbara B. Kahn, K Sreekumaran  Nair. Impact of type 1 diabetes and insulin treatment on plasma levels and fractional  synthesis  rate  of   retinol-binding  protein  4.  J  Clin  Endocrinol  Metab.  2009 Dec;94(12):5125-30
    14. Abdul Jaleel Short KR, Asmann YW, Klaus KA, Morse DM, Ford GC, Nair KS In vivo measurement of  synthesis rate of individual skeletal muscle mitochondrial proteins. Am J Physiol Endocrinol Metab 295(5):E1255-68, 2008
    15. Abdul Jaleel, Barbara A. L. Owen, Michelle K. Manske, Jerry A. Katzmann, Robert A. Kyle,  Roshini  S.  Abraham.  Bence  Jones  Cryoglobulinuria:  Characterization  of  a  Urinary Kappa Light Chain Cryoglobulin. Clinical Chemistry  52(7):1435-1436, 2006
    16. Abdul Jaleel, Vandana Nehra, Xuan-Mai T Persson, Yves Boirie, Maureen Bigelow, K Sreekumaran  Nair.  In  vivo measurement of synthesis rate of  multiple plasma proteins  in humans. Am J Physiol Endocrinol Metab  291(1):E190-7, 2006
    17. Abdul Jaleel , Panagiotis Halvatsiotis , Brian Williamson, Peter Juhasz , Stephen Martin and K.Sreekumaran N air Identification of amadori modified plasma proteins in type 2 diabetes and the effect of short-term intensive insulin treatment. Diabetes Care 28:645-652,2005
    18. Abdul Jaleel, K.Sreekumaran Nair: Identification of multiple proteins whose synthetic rates  are  enhanced  by  amino  acids  in  rat  hepatocytes.  Am  J  Physiol  Endocrinol  Metab 286:E950-E957, 2004.
    19. Nair K.S., Jaleel A, Asmann YW, Short KR, Raghavakaimal S:   Proteomic research: potential opportunities for clinical and physiological investigators. Am J Physiol Endocrinol: 286:E683-E874, 2004.
    20. Goswami R, Kochupillai N, Crock PA, Jaleel A, Gupta N. Pituitary autoimmunity in patients with Sheehan's syndrome. J Clin Endocrinol Metab 2002 Sep;87(9):4137-41
    21. R Goswami, A Jaleel, and N Kochupillai. Insulin antibody response to bovine insulin therapy:  functional  significance among insulin requiring young diabetics in India. Diabetes Research and Clinical Practice 2000; 49 (1) 7-15
    22. R   Goswami,   N   Jayasuryan,   A   Jaleel,   N   Tandon   and   N   Kochupillai.   Insulin autoantibodies  before and after Carbimazole therapy in Asian Indian patients with Grave’s disease. Diabetes Research and Clinical Practice 1998; 40 (3) 201-206
  • Team


    Dr. Mahesh S Krishna, Postdoctoral Fellow

    Dr. Mahesh S Krishna
    Dr. Mahesh S Krishna

    Dr. Mahesh S Krishna, Postdoctoral Fellow

    Aneesh Kumar A, PhD Student

    aneeshkumar
    aneeshkumar

    Aneesh Kumar A, PhD Student

    Ms. Anusree V R, Project Assistant

    AnusreeVR
    AnusreeVR

    Ms. Anusree V R, Project Assistant

  • Alumni