Profile

Research

Publications

Team

Alumni

Abdul Jaleel K. A, PhD

Scientist F

+91-471-2529540

jaleel@rgcb.res.in

abduljaleel
abduljaleel

Abdul Jaleel K. A, PhD

Scientist F

+91-471-2529540

jaleel@rgcb.res.in

  • Profile

    • Post Doctoral 2001-2006 (Mayo Clinic, Rochester, Minnesota, USA)
    • Ph.D 1998 (All India Institute of Medical Sciences / Jamia Millia Islamia, New Delhi)
    • M.Sc Medical Microbiology (Mahatma Gandhi University, Kottayam)
    • Dec 2015 to Present: Scientist - F, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram
    • Dec 2011 to Nov 2015: Scientist - E-II, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram
    • 2010 - 2011: Director of Laboratories & Research, Cellaram Diabetes Institute, Pune, India
    • 2007- 2010: Assistant Professor of Medicine, Mayo Clinic, Rochester, Minnesota, USA
    • 2007: Novel Methodology Development Award (NMDA) from Mayo Clinic, Center for Translational Science Activities (CTSA)
    • 2003: NIH Travel Grant for the meeting "Proteomics in Diabetes" NIDDK, NIH, Maryland
    • 1995: Senior Research Fellowship (UGC), India
    • 1993: Junior Research Fellowship (UGC), India
  • Research

    Metabolic transition processes associated with the onset of type 2 diabetes

    My research interest is in studying the mechanism of pathophysiology of type 2 diabetes. One area we want to focus is how independent risk factors for diabetes evolve over time in developing insulin resistance, which is the hallmark of type 2 diabetes. Type 2 diabetes is the most commonly diagnosed metabolic disease and metabolic alterations are often present years before the overt disease. Emerging technologies such as mass spectrometry have augmented the feasibility of acquiring high-throughput profiles of a whole organism's metabolic status (metabolite profiling, or metabolomics). Metabolomics allow assessment of large numbers of metabolites that are substrates, products and by-products of biochemical pathways of body metabolism. In addition to serving as potential biomarkers of disease, metabolites may have unexpected roles as regulatory signals with hormone-like functions or effectors of the pathogenesis of disease itself. At present we are developing mass spectrometry based tools of metabolomics in our laboratory. The present activity intend to identify the altered metabolic pathways in healthy people who are at risk of developing T2D (e.g., family history of T2D) by mass spectrometry based metabolomics. Towards this objective a project "Metabolic Profiling of Normal Healthy people in Kerala" is being executed by recruiting normal healthy people, but having various risk factors of T2D. We conduct human studies and besides mass spectrometry, we also employ various clinical, biochemical and hormonal measurements in the basal as well as postprandial blood of the study subjects.

    Regulation of Molecular Mechanism Underlying Adipogenesis through its mi-RNA Network

    We also have interest in investigating the molecular mechanism of adipogenesis as obesity and diabetes are highly associated due to interwoven molecular links. Adipogenesis in mammals is regulated genetically and hormonally. Adipogenic transcription factors, which regulate the expression of many adipogenic genes leading to the differentiation of adipocytes have been identified, such as peroxisome proliferator-activated receptor-gamma (PPAR-γ). PPAR- γ is the master regulator of adipogenesis and no factors have been discovered that promotes adipogenesis in the absence of PPAR-γ. So, inhibiting the activity of PPAR-γ can result in overall imbalance of body metabolism, energy balance and homeostasis. Recent research on adipocyte differentiation and maturation process has led to explain the role of micro RNA (miRNA) in controlling the whole process. The multi-functionality of PPAR- γ is supposed to be mediated through epigenetic mechanisms such as miRNA based modulation. In this contest, our lab is presently conducting studies on the modulation of upstream/downstream regulators of PPAR gamma using miRNA as molecular switches to reduce adipocyte hypertrophy and hyperplasia. Preliminary results so far carried out include the investigation of change in expression of PPAR γ, CEBPα, PGC1α, TNFα, FABP4 and Glut4 at different stages of adipocyte differentiation and compared with undifferentiated pre-adipocytes. Expression pattern of these genes in presence of PPAR gamma agonist (rosiglitazone), partial agonist (FMOC-L-Leucine) and antagonist (GW9662) were also checked in the initial phase of study.

  • Publications

    Publications

    1. Mahesh S. Krishna A. Aneesh Kumar, K A. Abdul Jaleel Time-dependent alterations in mRNA, protein and microRNA during in vitro adipogenesis. Molecular and Cellular Biochemistry 2018: DOI 10.1007/s11010-018-3307-y
    2. Azeez JM, Vini R, Remadevi V, Surendran A, Jaleel A, Santhosh Kumar TR, Sreeja S. VDAC1 and SERCA3 Mediate Progesterone-Triggered Ca2+ Signaling in Breast Cancer Cells. J Proteome Res. 2018 Jan 5;17(1):698-709. doi: 10.1021/acs.jproteome.7b00754.
    3. Vijayakumar G, Sreehari GK, Vijayakumar A and Jaleel A. Distinct Predictors and Co-morbidities in Early Onset Type 2 Diabetes Mellitus Among Asian Indians. Metabolic Syndrome and Related Disorders 2017;15(9):458-64
    4. Sengodan S, Rajan A, Hemalatha S, Nadhan R, Jaleel A, Srinivas P. Proteomic profiling of β-hCG induced spheres in BRCA1 defective triple negative breast cancer cells. J Proteome Res 2017 (DOI: 10.1021/acs.jproteome.7b00562)
    5. Abraham R, Singh S, Nair SR, Hulyalkar NV, Surendran A, Jaleel A, Srikanth J, Sreekumar E. Nucleophosmin (NPM1)/B23 in the Proteome of Human Astrocytic Cells Restricts Chikungunya Virus Replication. J Proteome Res 2017 DOI: 10.1021/acs.jproteome.7b00513
    6. Kumar V, Aneesh KA, Kshemada K, Ajith KGS, Binil RSS, Deora N, Sanjay G, Jaleel A, Muraleedharan TS, Anandan EM, Mony RS, Valiathan MS, Santhosh KTR, Kartha CC. Amalaki rasayana, a traditional Indian drug enhances cardiac mitochondrial and contractile functions and improves cardiac function in rats with hypertrophy. Scientific Reports. 2017;7(1):8588
    7. Kannan M, V. Aathmanathan S, Saravanakumar M, Jaleel A, Romanelli D, Tettamanti G, Krishnan M Proteomic analysis of the silkworm midgut during larva-pupa transition. Invertebrate Survival Journal 2016; 13: 191-204
    8. Mahadevan C, Krishnan A, Saraswathy GG, Surendran A, Jaleel A, Sakuntala M. Transcriptome- assisted label-free quantitativeproteomics analysis reveals novel insights into Piper nigrum - Phytophthora capsici phytopathosystem. Frontiers in Plant Science 2016;7:785
    9. Saritha VN, George J K, Jaleel A, Surendran A, Saravanakumar M, Kalavathy MC, Somanathan T, Rema P, Sujathan K. Analysis of differentially expressed proteins in the exfoliated cells of normal and squamous cell carcinoma of the uterine cervix to define candidate markers for cervical cancer. International Journal of Biochemistry and Biotechnology 2016; 5(1):626-36
    10. Muhamed J, Rajan A, Surendran A, Jaleel A, Anilkumar TV. Comparative profiling of extractable proteins in extracellular matrices of porcine cholecyst and jejunum intended for preparation of tissue engineering scaffolds. J Biomed Mater Res B Appl Biomater .  2015, doi: 10.1002/jbm.b.33567.
    11. Gopinath V, Raghunandanan A, Gomez RL, Jose L, Surendran A, Ramachandran R, Pushparajan AR, Mundayoor S, Jaleel A and Kumar RA. Profiling the proteome of Mycobacterium tuberculosis during dormancy and reactivation. Molecular & Cellular Proteomics . 2015;14(8):2160-76
    12. Mahadevan C, Jaleel A, Deb L, Thomas G, Sakuntala M. Development of an Efficient Virus Induced Gene Silencing Strategy in the Non-Model Wild Ginger-Zingiber zerumbet and Investigation of Associated Proteome Changes. PLoS One. 2015;10(4):e0124518
    13. Abraham R, Mudaliar P, Jaleel A, Srikanth J, Sreekumar E. High throughput proteomic analysis and a comparative review identify the nuclear chaperone, Nucleophosmin among the common set of proteins modulated in Chikungunya virus infection. J Proteomics . 2015;120:126-41
    14. Dharmaprakash A, Mutt E, Jaleel E, Ramanathan S and Thomas S. Proteome profile of a pandemic Vibrio parahaemolyticus SC192 strain in the planktonic and biofilm condition. Biofouling 2014;30(6):729-39
    15. Ammu Mathew , Ganapati Natarajan , Lauri Lehtovaara , Hannu Häkkinen, Ravva Mahesh Kumar, Venkatesan Subramanian, Abdul Jaleel,  Thalappil Pradeep. Supramolecular Functionalization and Concomitant Enhancement in Properties of Au25 Clusters. ACS Nano  2014;8(1):139-52
    16. Piotr Zabielski, G. Charles Ford, X. Mai Persson, Abdul Jaleel, Jerry D. Dewey and K. Sreekumaran Nair. Comparison of different mass spectrometry techniques in the measurement of L-[ring-13C6] phenylalanine incorporation into mixed muscle proteins.  Journal of Mass Spectrometry 48(2):269-75, 2013
    17. Abdul Jaleel, Gregory C. Henderson, Benjamin J. Madden, Katherine A. Klaus, Dawn M.  Morse,  Srinivas  Gopala,  and  K.  Sreekumaran  Nair.  Nair.  Identification  of  De  Novo Synthesized  and  Relatively  Older  Proteins  Accelerated  Oxidative  Damage  to  De  Novo Synthesized Apolipoprotein A-1 in Type 1 Diabetes.  Diabetes 59:2366–2374, 2010
    18. Abdul Jaleel, Klaus K, Morse D, Karakelides H, Irving BA, K. Nair KS. Differential Effects of Insulin Deprivation and Systemic Insulin Treatment on Plasma Protein Synthesis in Type 1 Diabetic People. Am J Physiol Endocrinol Metab 297: E889–E897, 2009
    19. Abdul Jaleel, Marion Jourdan, , Helen Karakelides, G. Charles Ford Barbara B. Kahn, K Sreekumaran  Nair. Impact of type 1 diabetes and insulin treatment on plasma levels and fractional  synthesis  rate  of   retinol-binding  protein  4.  J  Clin  Endocrinol  Metab.   2009 Dec;94(12):5125-30
    20. Abdul Jaleel Short KR, Asmann YW, Klaus KA, Morse DM, Ford GC, Nair KS In vivo measurement of  synthesis rate of individual skeletal muscle mitochondrial proteins. Am J Physiol Endocrinol Metab 295(5):E1255-68, 2008
    21. Abdul Jaleel, Barbara A. L. Owen, Michelle K. Manske, Jerry A. Katzmann, Robert A. Kyle,  Roshini  S.  Abraham.  Bence  Jones  Cryoglobulinuria:  Characterization  of  a  Urinary Kappa Light Chain Cryoglobulin. Clinical Chemistry   52(7):1435-1436, 2006
    22. Abdul Jaleel, Vandana Nehra, Xuan-Mai T Persson, Yves Boirie, Maureen Bigelow, K Sreekumaran  Nair.  In  vivo measurement of synthesis rate of  multiple plasma proteins  in humans. Am J Physiol Endocrinol Metab   291(1):E190-7, 2006
    23. Abdul Jaleel , Panagiotis Halvatsiotis , Brian Williamson, Peter Juhasz , Stephen Martin and K.Sreekumaran N air Identification of amadori modified plasma proteins in type 2 diabetes and the effect of short-term intensive insulin treatment. Diabetes Care 28:645-652,2005
    24. Abdul Jaleel, K.Sreekumaran Nair: Identification of multiple proteins whose synthetic rates  are  enhanced  by  amino  acids  in  rat  hepatocytes.  Am  J  Physiol  Endocrinol  Metab 286:E950-E957, 2004.
    25. Nair K.S., Jaleel A, Asmann YW, Short KR, Raghavakaimal S:   Proteomic research: potential opportunities for clinical and physiological investigators. Am J Physiol Endocrinol: 286:E683-E874, 2004.
    26. Goswami R, Kochupillai N, Crock PA, Jaleel A, Gupta N. Pituitary autoimmunity in patients with Sheehan's syndrome. J Clin Endocrinol Metab 2002 Sep;87(9):4137-41
    27. R Goswami, A Jaleel, and N Kochupillai. Insulin antibody response to bovine insulin therapy:  functional  significance among insulin requiring young diabetics in India. Diabetes Research and Clinical Practice 2000; 49 (1) 7-15
    28. R   Goswami,   N   Jayasuryan,   A   Jaleel,   N   Tandon   and   N   Kochupillai.   Insulin autoantibodies  before and after Carbimazole therapy in Asian Indian patients with Grave’s disease. Diabetes Research and Clinical Practice 1998; 40 (3) 201-206
  • Team


    Dr. Mahesh S Krishna, Postdoctoral Fellow

    We are focussed on the regulation of adipogenesis using micro RNA (miRNA) as a tool. Adipogenesis is a complicated process involving activation and interaction of various transcription factors and associated genes. PPAR gamma is considered as master regulator of adipogenesis. The differentiation of pre-adipocytes to adipocyte happens with the activation of C/EBPs, PPARs and other steroid responsive nuclear factors. Of these, activation of PPAR gamma leads to expression of most of the genes responsible for fatty acid metabolism, transport, glucose homeostasis and associated enzymes. PPAR gamma is a versatile protein with varied functions in body including inflammatory response, cell growth and is linked to diseases like diabetes, obesity, cancer, arthritis etc. Here, we are trying to modulate the functional roles of PPAR gamma by regulating the binding or expressional pattern of co- factors associated with this, using the translational repressor potential of micro RNA.

    Dr. Mahesh S Krishna
    Dr. Mahesh S Krishna

    Dr. Mahesh S Krishna, Postdoctoral Fellow

    We are focussed on the regulation of adipogenesis using micro RNA (miRNA) as a tool. Adipogenesis is a complicated process involving activation and interaction of various transcription factors and associated genes. PPAR gamma is considered as master regulator of adipogenesis. The differentiation of pre-adipocytes to adipocyte happens with the activation of C/EBPs, PPARs and other steroid responsive nuclear factors. Of these, activation of PPAR gamma leads to expression of most of the genes responsible for fatty acid metabolism, transport, glucose homeostasis and associated enzymes. PPAR gamma is a versatile protein with varied functions in body including inflammatory response, cell growth and is linked to diseases like diabetes, obesity, cancer, arthritis etc. Here, we are trying to modulate the functional roles of PPAR gamma by regulating the binding or expressional pattern of co- factors associated with this, using the translational repressor potential of micro RNA.

    Dr. Kalaivani V, National Post-Doctoral Fellow (N-PDF, DST-SERB)

    Effects of O-glycans of Apo(a) on Angiogenesis and its Implications in Pre-eclampsia.

    Pre-eclampsia (PE), a medical condition in which hypertension arises in pregnancy, is the 3rd leading cause of maternal mortality and the 7th leading cause of the perinatal mortality. The pathogenesis of PE is caused by poor placentation with impaired angiogenesis of maternal spiral arteries leading to intra-uterine growth restriction. Lipoprotein (a) [Lp(a)] deposits were found on the placenta that had undergone acute-atherosis, another characteristic event of PE. Moreover, an increased plasma Lp(a) level was observed in PE subjects. Presence of apolipoprotein (a) [apo(a)], a unique glycoprotein subunit of Lp(a), on diseased placenta along with its emerging role as an anti-angiogenic agent suggests its involvement in PE pathogenesis. However, the exact domain of apo(a) behind this effect is poorly understood. The extended O-glycan structure of apo(a) is a strong ligand for galectin-1, a pro-angiogenic, O-glycan specific lectin abundant in placenta. In vivo studies have shown that the inhibition of galectin-1-mediated angiogenesis leads to poor placentation and provokes PE-like symptoms. There are very few studies which addressed physiologic or pathologic functions of apo(a) O-glycans. In this context, the present project aims to identify the role of the extended O-glycan structure of apo(a) in the modulation of galectin-1- mediated angiogenesis associated with pre-eclampsia.

    Dr. Mahesh S Krishna
    Dr. Mahesh S Krishna

    Dr. Kalaivani V, National Post-Doctoral Fellow (N-PDF, DST-SERB)

    Effects of O-glycans of Apo(a) on Angiogenesis and its Implications in Pre-eclampsia.

    Pre-eclampsia (PE), a medical condition in which hypertension arises in pregnancy, is the 3rd leading cause of maternal mortality and the 7th leading cause of the perinatal mortality. The pathogenesis of PE is caused by poor placentation with impaired angiogenesis of maternal spiral arteries leading to intra-uterine growth restriction. Lipoprotein (a) [Lp(a)] deposits were found on the placenta that had undergone acute-atherosis, another characteristic event of PE. Moreover, an increased plasma Lp(a) level was observed in PE subjects. Presence of apolipoprotein (a) [apo(a)], a unique glycoprotein subunit of Lp(a), on diseased placenta along with its emerging role as an anti-angiogenic agent suggests its involvement in PE pathogenesis. However, the exact domain of apo(a) behind this effect is poorly understood. The extended O-glycan structure of apo(a) is a strong ligand for galectin-1, a pro-angiogenic, O-glycan specific lectin abundant in placenta. In vivo studies have shown that the inhibition of galectin-1-mediated angiogenesis leads to poor placentation and provokes PE-like symptoms. There are very few studies which addressed physiologic or pathologic functions of apo(a) O-glycans. In this context, the present project aims to identify the role of the extended O-glycan structure of apo(a) in the modulation of galectin-1- mediated angiogenesis associated with pre-eclampsia.

    Aneesh Kumar A, PhD Student

    We believe that understanding more about the molecular pathogenesis of type 2 diabetes in the Indian population is essential since having a unique Asian-Indian phenotype is more susceptible with respect to the west and type 2 diabetes is reaching an epidemic level in India comparable to the Western world. Factors such as preferential deposition of body fat in the visceral depot, lipodystrophy, ectopic fat deposition in the liver, and chronic low-grade inflammatory response to adiposity are reported as causative factors that increase risk for metabolic disorders in the Indian population. In this scenario, it can be assumed that there exists a distinct metabolic transition state leading to a pre-diabetes state well in advance, in those who are at the risk of developing diabetes. My objective is in performing mass spectrometry based metabolomics using blood samples to identify such altered metabolic pathways from healthy study participants who are at the risk of developing diabetes. The emerging era of omics technologies provides opportunities to study complex biological processes at a systems level. Metabolomics is the most recent omic approach and is believed to have an ability to provide the ultimate molecular phenotype compared to other omics.

    aneeshkumar
    aneeshkumar

    Aneesh Kumar A, PhD Student

    We believe that understanding more about the molecular pathogenesis of type 2 diabetes in the Indian population is essential since having a unique Asian-Indian phenotype is more susceptible with respect to the west and type 2 diabetes is reaching an epidemic level in India comparable to the Western world. Factors such as preferential deposition of body fat in the visceral depot, lipodystrophy, ectopic fat deposition in the liver, and chronic low-grade inflammatory response to adiposity are reported as causative factors that increase risk for metabolic disorders in the Indian population. In this scenario, it can be assumed that there exists a distinct metabolic transition state leading to a pre-diabetes state well in advance, in those who are at the risk of developing diabetes. My objective is in performing mass spectrometry based metabolomics using blood samples to identify such altered metabolic pathways from healthy study participants who are at the risk of developing diabetes. The emerging era of omics technologies provides opportunities to study complex biological processes at a systems level. Metabolomics is the most recent omic approach and is believed to have an ability to provide the ultimate molecular phenotype compared to other omics.

    Ms. Anusree V R, JRF

    In India, most people adhere to a vegetarian diet, which may lead to cobalamin deficiency. Vitamin B12 is involved in the metabolism of every cell of the human body and is essential in DNA synthesis and cellular energy production. The Vitamin B12 deficiencies are subtle and often not recognized. Vitamin B12 deficiency has gained high attention due to their possible association with the nervous system and mood disorders, birth and reproductive defects and cardiovascular diseases. Vitamin B12 is an essential co-factor in the methionine- homocysteine metabolism, and its deficiency leads to elevated levels of homocysteine. In this context, the study of Vitamin B12 deficiency and the understanding of the process behind the hyper-homocysteinemia related to Vitamin B12 and folic acid deficiency is essential. My objective is to identify any metabolic alterations in sub-clinical Vitamin B12 deficiency using mass spectrometry-based metabolomics.

    AnusreeVR
    AnusreeVR

    Ms. Anusree V R, JRF

    In India, most people adhere to a vegetarian diet, which may lead to cobalamin deficiency. Vitamin B12 is involved in the metabolism of every cell of the human body and is essential in DNA synthesis and cellular energy production. The Vitamin B12 deficiencies are subtle and often not recognized. Vitamin B12 deficiency has gained high attention due to their possible association with the nervous system and mood disorders, birth and reproductive defects and cardiovascular diseases. Vitamin B12 is an essential co-factor in the methionine- homocysteine metabolism, and its deficiency leads to elevated levels of homocysteine. In this context, the study of Vitamin B12 deficiency and the understanding of the process behind the hyper-homocysteinemia related to Vitamin B12 and folic acid deficiency is essential. My objective is to identify any metabolic alterations in sub-clinical Vitamin B12 deficiency using mass spectrometry-based metabolomics.

    Ms. Gopika Satheesh, JRF

    There is a global epidemic of Type 2 Diabetes Mellitus (T2DM); it is estimated that Indians have the highest worldwide prevalence of diabetes and the number of affected individuals is expected to be doubled in the coming years. Metabolic diseases like T2DM are often latent for a while prior to becoming clinically evident. A majority of patients are asymptomatic until T2DM has reached an advanced stage. Similarly, it is likely that the metabolic pathways are altered beforehand of a prediabetic state. Thus, it is critical to identify individuals before the onset of T2DM because preventive therapies do exist and also associated complications accumulate over time. In order to develop preventive strategies, identification of early metabolic alterations is required to study etiological pathways and also to recognize high-risk individuals. Therefore a prospective study using tools of metabolomics is the most suitable way to predict the metabolic shift leading to T2DM. My work focuses on conducting mass spectrometry-based metabolomics using blood samples collected from the participants who were part of the previous metabolomics study conducted five years ago.

    AnusreeVR
    AnusreeVR

    Ms. Gopika Satheesh, JRF

    There is a global epidemic of Type 2 Diabetes Mellitus (T2DM); it is estimated that Indians have the highest worldwide prevalence of diabetes and the number of affected individuals is expected to be doubled in the coming years. Metabolic diseases like T2DM are often latent for a while prior to becoming clinically evident. A majority of patients are asymptomatic until T2DM has reached an advanced stage. Similarly, it is likely that the metabolic pathways are altered beforehand of a prediabetic state. Thus, it is critical to identify individuals before the onset of T2DM because preventive therapies do exist and also associated complications accumulate over time. In order to develop preventive strategies, identification of early metabolic alterations is required to study etiological pathways and also to recognize high-risk individuals. Therefore a prospective study using tools of metabolomics is the most suitable way to predict the metabolic shift leading to T2DM. My work focuses on conducting mass spectrometry-based metabolomics using blood samples collected from the participants who were part of the previous metabolomics study conducted five years ago.

    Akhila Suresh S , Project Assistant

    My work includes generating and submitting indents, CRV forms & consumables data sheets. Receiving the consumables from stores and maintaining the sub stocks provided. Updating the chemicals indented and received till date. Assistance in the administration work including settling of advances, updating fund details

    AnusreeVR
    AnusreeVR

    Ms. Akhila Suresh S , Project Assistant

    My work includes generating and submitting indents, CRV forms & consumables data sheets. Receiving the consumables from stores and maintaining the sub stocks provided. Updating the chemicals indented and received till date. Assistance in the administration work including settling of advances, updating fund details

  • Alumni