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Ani V Das, PhD

Program Scientist

+91-471-2529587

anivdas@rgcb.res.in

ani
ani

Ani V Das, PhD

Program Scientist

+91-471-2529587

anivdas@rgcb.res.in

  • Profile

    • 2000-2006: Post-doctoral research Associate (Department of Ophthalmology & Visual sciences, University of Nebraska Medical Center, Omaha, USA)
    • 1996-2000: Ph.D. Biotechnology (Cochin University of Science & Technology, Cochin, India)
    • 1992-1994: M.Sc. Biotechnology (Cochin University of Science & Technology, Cochin, India)
    • 1989-1992: B.Sc. Botany (University of Calicut, India)
    • Awarded DST Fast Track Grant for Young Scientists: Jan 2010
    • Awarded Council for Scientific and Industrial Research (CSIR) Senior Research Fellowship from 1998 to 2000
    • Awarded Cochin University of Science & Technology, Government of India Junior Research Fellowship from 1996 to 1998
    • 1994: M.S. Biotechnology 3rd Rank, Cochin University of Science & Technology
    • 1992: B.S. Botany 4th Rank, Calicut University
    • Member of ARVO, 2000-2006
    • Member of Society for Neuroscience, 2003
    • Member of Society for Biotechnologist, India, (since 1998)
  • Research

    Germ cell tumors (GCTs) are malignant or non-malignant tumors that represent a histologically heterogeneous group of neoplasms derived from the germ-cell lineage. Despite their heterogeneity, they are all presumed to arise from totipotent primordial germ cells (PGC). Most of the ovarian and testicular cancers are of germ cell origin. Histologically, GCTs can be divided into germinomas and non-germinomas. Germinomas (GERs; also called seminomas in the testis and dysgerminomas in the ovary) are tumors of undifferentiated germ cells that retain markers of pluripotency. In contrast, non-germinomas undergo differentiation to resemble somatic-type tissues (teratomas) or extra-embryonic structures (yolk sac tumor (YST) and choriocarcinoma. Nonseminomas contain one or more histological subtypes representing various differentiation lineages and stages of embryonic development. Embryonal carcinoma cells represent the stem cell component, which has the potential to differentiate towards embryonic and extra-embryonic tissues. Though GCTs are rare, they account for about 2-4% of pediatric cancers and cancers that occur in adolescents. These tumors can spread to the other parts of body such as lungs, liver, lymph nodes, central nervous system. GCTs are often highly sensitive to chemotherapy and 80% cure can be achieved with cisplatin (CDDP)-based combination chemotherapy followed by secondary resection in the case of residual tumor lesions. But mature teratomas, despite an identical genetic constitution, do not share the general chemosensitivity of GCTs. Due to intrinsic chemotherapy resistance, mature teratomas can be found in - 30 - 40% of residual lesions after chemotherapy. Ten to thirty percent of patients with metastatic GCT were unable to achieve a durable complete remission after initial treatment, either due to incomplete response or relapse. Based on this clinical background, understanding of the mechanisms of chemosensitivity and resistance of tumor cells is critical to further improve therapeutic outcome. A more accurate prediction of treatment outcome may help to avoid under or overtreatment. My work aims to elucidate mechanisms involved in regulation of multidrug resistant proteins in pluripotent stem cells. We anticipate that information generated on specific cellular pathways evolve, it may allow options for reversal or overcoming treatment resistance mechanisms.

  • Publications

    1. Sreekumaran Sreekanth, Vazhanthodi A Rasheed, Lalitha Soundararajan, Jayesh Antony, Minakshi Saikia, Krishnankutty Chandrika Sivakumar, Ani V Das. miR Cluster 143/145 Directly Targets Nrl and Regulates Rod Photoreceptor Development. Molecular Neurobiology, 1-17, 2016. DOI: 10.1007/s12035-016-0237-0.
    2. Ani V Das* and M. Radhakrishna Pillai. Implications of miR cluster 143/145 as universal anti-oncomiRs and their dysregulation during tumorigenesis.Cancer Cell Int (2015) 15:92. * Corresponding author.
    3. M. Sajimol Augustine, Abdulaziz Anas, Ani V. Das, S. Sreekanth, S and Jayalekshmi. (2015) Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 136: 327-333.
    4. Rasheed VA, Sreekanth S, Dhanesh SB, Divya MS, Divya TS, Akhila PK, Subashini C, Sivakumar KC, Das AV* and James J.(2014) Developmental wave of Brn3b expression leading to RGC fate specification is synergistically maintained by miR-23a and miR-374.Dev Neurobiol . Doi:10.1002/dneu.22191. *Co-Corresponding author
    5. Sreekanth S and Das AV*. (2012) Functional Evaluation of microRNAs in the Retinal Development: Role of miR Cluster 143/145 in the Regulation of Photoreceptor Differentiation. Int J Deb Neurosci. DOI:10.1016/j.ijdevneu.2012.03.257. * Corresponding author.
    6. Ahmad I, Del Debbio CB, Das AV, Parameswaran S. (2011) Muller Glia: A promising target for therapeutic regeneration. Invest Ophthalmol Vis Sci, Jul; 52(8):5758-64.
    7. B.Jagatha, MS. Divya, R.Sanalkumar, CL. Indulekha, S. Vidyanand, TS. Divya, AV. Das and Jackson James. (2009). In vitro differentiation of retinal ganglion-like cells from embryonic stem cell derived neural progenitors. Biochem Biophys Res Commun. Mar 6;380(2):230-5.
    8. Bhattacharya S, Das AV, Mallya K and Ahmad I (2008). CNTF-mediated signaling regulates neuronal versus glial differentiation of retinal stem cells/progenitors by concentration-dependant recruitment of MAPK and Jak-STAT pathways in conjunction with Notch signaling. Stem Cells. Oct; 26(10): 2611-24.
    9. Das AV, Bhattacharya S, Zhao X, Hegde G, Mallya K and Ahmad I (2008). The canonical Wnt pathway regulates retinal stem cells/progenitors in concert with Notch signaling. Dev. Neuroscience. Nov; 30(6): 389-409.
    10. Zhao X, Das AV, Bhattacharya S, Mallya KB and Ahmad I. (2008). Generation of neurons with functional properties from limbal epithelium; an autologous cell therapy approach for photoreceptor degeneration. Stem Cells. Apr;26(4):939-49.
    11. Das AV, James J, Sumitra Bhattacharya, Imbalzano AN, Hegde G, Zhao X, Mallya K, Ahmad F, Knudsen E and Ahmad I. (2007). SWI/SNF Chromatin Remodeling ATPase Brm Regulates the Differentiation of Early Retinal Stem Cells/Progenitors by Influencing Brn3b Expression and Notch Signaling. J. Biol. Chem. Nov 30; 282(48):35187-201.
    12. Bhattacharya S, Das AV, Mallya K and Ahmad I. (2007). Maintenance of retinal stem cells by Abcg2 is regulated by Notch signaling. J Cell Sci. Aug 1;120(Pt 15):2652.
    13. Hegde GV, James J, Das AV, Zhao X, Bhattacharya S and Ahmad I. (2007) Characterization of early progenitor micro environment: Presence of activities selective for the differentiation of retinal ganglion cells and maintenance of progenitors. Exp. Eye Res. 84(3): 577-590.
    14. Das AV, Mallya KB, Zhao X, Ahmad F, Bhattacharya B, Thoreson WB, Hegde GV and Ahmad I. (2006) Neural Stem Cell Properties Of Müller Glia In The Mammalian Retina: Regulation By Notch And Wnt Signaling. Dev Biol. 299 (1): 283-302.
    15. Das VA, Chathu F, Paulose CS (2006)Decreased alpha(2)-adrenergic receptor in the brain stem and pancreatic islets during pancreatic regeneration in weanling rats. Life Sci. 79(16): 1507-13.
    16. Das VA, Robinson R, Paulose CS. (2006) Enhanced beta-adrenergic receptors in the brain and pancreas during pancreatic regeneration in weanling rats.Mol Cell Biochem. 289(1-2): 11-19.
    17. Ani Das V, Savitha B, Paulose CS. (2006) Decreased alpha(1)-Adrenergic Receptor Binding in the Cerebral Cortex and Brain Stem during Pancreatic Regeneration in Rats. Neurochem Res. 31(6):727-34.
    18. Das AV, Zhao X, James J, Kim M, Cowan KH, Ahmad I. (2006) Neural stem cells in the adult ciliary epithelium express GFAP and are regulated by Wnt signaling. Biochem Biophys Res Commun. 339(2):708-16.
    19. Das AV, Edakkot S, Thoreson B, James J, Bhattacharya S, Ahmad I (2005) Membrane properties of retinal stem cells/progenitors. Prog Retin Eye Res. 24(6): 663-81. Review.
    20. Das AV, James J, Rahnenfuhrer J, Thoreson B, Bhattacharya S, Zhao X, Ahmad I (2005) Retinal properties and potential of the adult mammalian ciliary epithelium stem cells. Vision Res. 2005 Jun;45(13):1653-66.
    21. Zhao X, Das AV, Soto-Leon F, Ahmad I (2005) Growth factor-responsive progenitors in the postnatal mammalian retina. Dev Dyn. 2005; 232(2): 349-58.
    22. Das, AV, Zhao, X, and Ahmad, I (2005) Stem cell therapy for retinal degeneration: retinal neurons from heterologous sources. Sem. Ophthalmol. 20(1): 3-10. Review.
    23. Renuka TR, Ani Das V, Paulose CS. (2004) Alterations in the muscarinic M1 and M3 receptor gene expression in the brain stem during pancreatic regeneration and insulin secretion in weanling rats.Life Sci. 24;75(19):2269-80
    24. James J, Das AV, Rahnenfuhrer J, Ahmad I (2004) Cellular and molecular characterization of early and late retinal stem cells/progenitors: differential regulation of proliferation and context dependent role of Notch signaling. J Neurobiol. 61(3): 359-76.
    25. Bhattacharya S, Dooley C, Soto-Leon, F, Madson J, Das AV, Ahmad I (2004) Involvement of Ath3 in CNTF-mediated differentiation of the late retinal progenitors. Mol Cell Neurosci. 27(1): 32-43.
    26. Das AV, James J, Zhao X, Rahnenfuhrer J, Ahmad I (2004) Identification of c-Kit receptor as a regulator of adult neural stem cells in the mammalian eye: interactions with Notch signaling. Dev Biol. 273(1):87-105.
    27. Ahmad I, Das AV, James J, Bhattacharya S, Zhao X (2004) Neural stem cells in the mammalian eye: types and regulation. Semin Cell Dev Biol. 15(1): 53-62. Review.
    28. James J, Das AV, Bhattacharya S, Chacko DM, Zhao X, Ahmad I (2003) In vitro generation of early-born neurons from late retinal progenitors. J Neurosci. 23(23): 8193-203.
    29. Bhattachrya S, Jackson JD, Das AV, Thoreson B,Kuszynski C, James J, Joshi S, Ahmad I (2003) Direct identification and enrichment of retinal stem cells/progenitors by Hoechst dye efflux assay. Invest Ophthalmol Vis Sci. 44(6): 2764-73.
    30. Chacko DM, Das AV, Zhao X, James J, Bhattacharya S, Ahmad I (2003) Transplantation of ocular stem cells: the role of injury in incorporation and differentiation of grafted cells in the retina. Vision Res. 43(8):937-46.
    31. Zhao X, Das AV, Thoreson WB, James J, Wattnem TE, Rodriguez-Sierra J, Ahmad I (2002) Adult corneal limbal epithelium: a model for studying neural potential of non-neural stem cells/progenitors. Dev Biol. 2002; 15;250(2):317-31.
    32. Das AV, Padayatti PS, Paulose CS. Effect of leaf extract of Aegle marmelose (L.) Correa ex Roxb. on histological and ultrastructural changes in tissues of streptozotocin induced diabetic rats.Indian J Exp Biol. 1996; 34(4):341-5.

    Book Chapter

    1. Das, AV, Jackson James, Sreekumaran Edakkot and Ahmad, I (2004) Retinal stem cells: In Stem cells and CNS development. Neural Development and Stem Cells 2nd edition, edited by Mahendra S. Rao, M.D., .Humana Press.
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