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Team

Alumni

Arumugam Rajavelu, PhD

DST-INSPIRE faculty

+91-471-2529598

arajavelu@rgcb.res.in

Arumugam-Rajavelu
Arumugam-Rajavelu

Arumugam Rajavelu, PhD

DST-INSPIRE faculty

+91-471-2529598

arajavelu@rgcb.res.in

  • Profile

    • Ph.D Biochemistry (2008 – 2011), Jacobs University Bremen, Germany
    • M.Sc Microbiology (Faculty of Medicine) (2002 – 2005), Dr. ALM PGIBMS Taramani campus, University of Madras, Chennai, India
    • B.Sc Microbiology (1999 – 2002), Periyar University, Salem, India
    • Dec 2013 – Till date, DST-INSPIRE Faculty at Rajiv Gandhi Center for Biotechnology (RGCB), Trivandrum, Kerala, India
    • Post doctoral fellow (Dec 2011 – Nov 2013), Laboratory of Prof. Albert Jeltsch, Stuttgart University, Stuttgart, Germany
    • Junior Research fellow (Dec 2005 – June 2008), Laboratory of Prof G. Padmanaban and Prof P.N. Rangarajan, Department of Biochemistry, Indian Institute of Science (IISc), Bangalore, India
    • Trainee Scientist (July 2005 – Nov 2005), Jubilant Biosys, Bangalore, India
    • DBT – Innovative Young Biotechnologist Award (IYBA) 2014, from Department of Biotechnology, Govt of India.
    • DST-INSPIRE faculty award (Aug 2013) from Department of Science and Technology, Govt of India
    • Postdoctoral Fellowship from Stuttgart University, funded by German Research Foundation (DFG), Stuttgart, Germany
    • PhD Fellowship funded by German Research Foundation (DFG), Germany (July 2008 – Sep 2011). PhD awarded with Special Distinction
    • DBT JRF fellowship from Indian Institute of Science (IISc), funded by Dept of Biotechnology, Govt of India

     

    Funding agencies

    1. Department of Science & Technology (DST), Govt of India, India

    Journals

    1. Oncogene
    2. BMC Biochemistry
    3. Chemosphere
    4. European Journal of Clinical Investigation
    5. JoVE
    6. Microbiology and Immunology
  • Research

    Malaria is an infectious disease caused by apicomplexan parasite, till date five Plasmodium species P. falciparum, P. vivax, P. Malariae, P. ovale and P. knowlesi have been identified which infects humans. Among the five species, the P. falciparum causes the most severe form of malaria. The female anopheles mosquito injects 20-100 sporozoites to human dermis to initiate the infection, these sporozoites reaches to liver via blood stream. In liver the parasites replicate and release the merozoites into blood stream, which further infects RBC and continues as asexual intraerythrocytic developmental cycle (IDC). The asexual growth of parasite consist different stages, including rings, trophozoites and schizonts, the mature schizonts ruptures the RBC and releases upto 30 merozoites that can invade into new RBCs and continue as asexual life cycle. The clinical manifestations of malarial disease occur during the IDC cycle of P. falciparum, which includes the severe form of cerebral malaria. The recent evidences indicates that P. falciparum under goes massive changes in “transcriptional activity´´ during IDC cycle, which suggested the role of epigenetic players at different stages of parasite development.

    Research work in my laboratory focuses on malarial biology and malarial epigenetics. We work on the tight regulation of chromatin in malarial parasite during its development in RBC and identification of modifications on the RNA in the parasites. We are also interested in understand the epigenetic protein’s role in differential gene expression in parasites, which eventually pave way to identify the new drug targets in the deadliest malarial parasites.

    Functional role of RNA modifications in Plasmodium falciparum (DST-INSPIRE funded)

    Modifications of RNA control the protein synthesis in mammals as well as protozoan parasites. We are interested to study the role of RNA modifications in malarial parasite’s growth and its development. Using biochemical and molecular approach, we will find out the possible modifications present in the RNA of P. falciparum and its role in protein synthesis at different stages of malarial parasites.

    Tight regulation of chromatin in human malarial parasites

    The major portion of P. falciparum’s genes exists as euchromatin when compared to higher order eukaryotes. The malarial parasite’s chromatin is tightly regulated in order to achieve the optimal expression of genes at different stages of parasites during IDC cycle. Here we are interested to study the role of post translational modifications on the histone proteins and its role in var gene expression and chromatin dynamics during IDC cycle of parasites.

    Extramural Funding

    1. Department of Science & Technology, Govt of India, India
    2. Department of Biotechnology, Govt of India, India
    3. Kerala State Science & Technology, Govt of Kerala, Kerala, India

    Intramural Funding

    1. Rajiv Gandhi Centre for Biotechnology (RGCB), TVM, Kerala
  • Publications

    Total number of Research publications: 18

    Cumulative impact factor: 83.1
    Total number of citations: 630 (Jan 2016)
    H-index: 13

    Selected Research publications

    1. Deplus R#, Blanchon L#, Rajavelu A#, Boukaba H#et al (2014). Regulation of DNA methylation patterns by CK2 mediated phosphorylation of Dnmt3a. Cell Reports. in press.
      # Equal first authors
    2. Bashtrykov P, Rajavelu A, Hackner B, Ragozin S, Carell T, Jeltsch A (2014). Targeted mutagenesis results in an activation of DNA methyltransferase 1 and confirms an autoinhibitory role of its RFTS domain. ChemBioChem. 9(3), 743-8
    3. Ceccaldi A#, Rajavelu A#, Ragozin S, Senamaud-Beaufirt C, Bashtrykov P, Testa N, Dali-Ali H, Maulay-Bailly C, Amand S, Guianvarc’h D, Jeltsch A, Arimondo PB (2013). Identification of Novel Inhibitors of DNA methylation by Screening of a Chemical Library. ACS Chemical Biology, 8(3), 543-8. # Equal first authors
    4. Rajavelu A, Jurkowska R, Fritz J, Jeltsch A (2012). Function and disruption of DNA Methyltransferase 3a cooperativ DNA binding and nucleoprotein filament formation. Nucleic Acid Res, 40(2): 569-80.
    5. Jurkowska R#, Rajavelu A# Anspach N, Urbanke C, Jankevicius G, Ragozin S, Nellen W, Jeltsch A (2011). Oligomerization and Binding of the Dnmt3a DNA Methyltransferase to Parallel DNA Molecules: Heterochromatic localization and role of Dnmt3L. J Biol Chem. 286 (27); 24200-7.# Equal first authors
    6. Siddique AN#, Nunna S#, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt R, Rots MG, Jurkowski T & Jeltsch A. Targeted methylation and gene silencing of VEGF-A in human cells by using a Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity J Mol Biol.425(3), 479-91. # Equal first authors
    7. Rajavelu A, Tulyasheva Z, Jaiswal R, Jeltsch A*, Kuhnert N* (2011). The inhibition of the mammalian DNA methyltransferase 3a (Dnmt3a) by dietary black tea and coffee polyphenols. BMC Biochem. 12; 16. [Highly accessed]
    8. Ceccaldi A, Rajavelu A, Champion C, Rampon C, Jurkowska R, Jankevicius G, Sénamaud-Beaufort C, Ponger L, Gagey N, Dali Ali H, Tost J, Vriz S, Ros S,Dauzonne D, Jeltsch A, Guianvarc’h D, Arimondo PB (2011). C5-DNA methyltransferase Inhibitors: From Screening to Effects on Zebrafish Embryo Development. Chembiochem. 14; 12 (9); 1337-45.
    9. Dhayalan A, Rajavelu A, Rathert P, Tamas R, Jurkowska RZ, Ragozin S, Jeltsch A (2010). The Dnmt3a PWWP domain reads histone 3 lysine 36 trimethylation and guides DNA methylation. J Biol Chem. 285(34); 26114-20.
    10. Nagaraj VA, Arumugam R, Chandra NR, Prasad D, Rangarajan PN, Padmanaban G (2009). Localisation of Plasmodium falciparum uroporphyrinogen III decarboxylase of the heme-biosynthetic pathway in the apicoplast and characterisation of its catalytic properties. Int J Parasitol. 39(5):559-68.
    11. Nagaraj VA, Arumugam R, Gopalakrishnan B, Jyothsna YS, Rangarajan PN, Padmanaban G (2008). Unique properties of Plasmodium falciparum porphobilinogen deaminase. J Biol Chem. 283(1): 437-44.
    12. Nagaraj VA, Arumugam R, Gopalakrishnan B, Jyothsna YS, Rangarajan PN, Padmanaban G (2008). Unique properties of Plasmodium falciparum porphobilinogen deaminase. J Biol Chem. 283(1): 437-44.
    SI. NoTitleAuthor’s Name PublisherYear of publication
    1Part V: Cell Function and Metabolism
    The Inhibition of the Mammalian DNA Methyltransferase 3a (Dnmt3a) by Dietary Black Tea and Coffee Polyphenols
    Arumugam Rajavelu, Zumrad Tulyasheva, Rakesh Jaiswal, Albert Jeltsch, and Nikolai Kuhnert Nutritional Biochemistry Apple Academic Press (AAP 2015
  • Team


    Jaya Mary Thomas, PhD Student

    Arteriovenous malformations (AVM) are tangles of dysplastic blood vessels which shunt blood from arteries to veins with no intervening capillary bed. AVM can occur in different organs such as liver, lungs, brain etc. Cerebral AVMs, are important causal factors of intracranial hemorrhage which may result in permanent disability or death, if not corrected surgically. The molecular milieu of this disease is complicated and pathogenesis of these malformations remains an enigma. In this context I like to focus on combination of genetic and epigenetic alterations of angiogenic specification and molecules conferring vascular stability in the pathogenesis of AVM.

    jaya mary
    jaya mary

    Jaya Mary Thomas, PhD Student

    Arteriovenous malformations (AVM) are tangles of dysplastic blood vessels which shunt blood from arteries to veins with no intervening capillary bed. AVM can occur in different organs such as liver, lungs, brain etc. Cerebral AVMs, are important causal factors of intracranial hemorrhage which may result in permanent disability or death, if not corrected surgically. The molecular milieu of this disease is complicated and pathogenesis of these malformations remains an enigma. In this context I like to focus on combination of genetic and epigenetic alterations of angiogenic specification and molecules conferring vascular stability in the pathogenesis of AVM.

  • Alumni