Profile

Research

Publications

Team

Alumni

Debasree Dutta, PhD

Scientist E-I

+91-471-2529597

debasreedutta@rgcb.res.in

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daba

Debasree Dutta, PhD

Scientist E-I

+91-471-2529597

debasreedutta@rgcb.res.in

  • Profile

    • Ph.D Science (Jadavpur University)
    • M.Tech Biotechnology (Jadavpur University)
    • M.Sc Biochemistry (University of Kalyani)
    • B.Sc Chemistry (University of Calcutta)
    • March 2012-Till date, Scientist C at RajivGandhi Centre for Biotechnology, Trivandrum-14, Kerala
    • 2007 October- 2012 March, Postdoctoral Fellow at University of Kansas Medical Centre, Kansas City, USA
    • 2007 August-2007 September, Research Associate at Jadavpur University, Kolkata
    • International travel grant from DBT for attending ISSCR 2013 at Boston, USA
    • DST Fast-track fellowship
    • American Heart Association Postdoctoral Fellowship (AHA), Midwest Affiliate, USA
    • Best poster at 8th Annual Greenwald Symposium on Reproductive Biology, USA
    • Fellow, Agricultural Research Scientist National Eligibility Test (ARS NET), Indian Council for Agricultural Research
    • Fellow, State Level Eligibility Test (SLET), University Grants Commission
    • Fellow, Council of Scientific and Industrial Research through National Eligibility Test (NET)
    • Associate member of International Society for Stem Cell Research (ISSCR)
    • Member of North American Vascular Biology Organization (NAVBO)
    • Member of Society for Developmental Biology (SDB)
    • Member of American Heart Association (AHA)
    • Life member of Indian Association of Cancer Research (IACR)
    • Life Member of Kerala Academy of Sciences, India
    • "Compositions and methods for establishing and maintaining stem cells in an undifferentiated state" US patent, Application Number: 2012/0270,313; Publication Date: 25/10/2012
  • Research

    To understand the molecular mechanisms regulating induction of pluripotency in differentiated cells:

    Microtubules are a major cytoskeletal component with many important functions. They are involved in cell division, maintenance of cell shape and polarity and transport of nutrients and organelles. Nucleation stage or the formation of oligomers is a crucial intermediate stage of microtubule assembly from its component proten tubulin. In a cell, improper nucleation may give rise to lack of polarity of interphase and mitotic cell, distorted shape and loss of directionality of microtubule mediated transport. Many proteins and antimitotic agents influence the nucleation stage of microtubule assembly.

    Embryonic fibroblasts upon transduced with transcription factors Oct4, Klf4, Sox2 and c-Myc generated induced pluripotent stem cells (iPSCs) similar in characteristics to embryonic stem cells (ESCs). Till now, they have been derived from different species and from other somatic cell populations with varied protocols. Their derivation is ethically and legally less problematic and technically more feasible. Reprogramming cells to a pluripotent state entails global epigenetic remodelling and introduces epigenetic changes, some of which are necessary for reprogramming to occur and others of which are inadvertently introduced during the process. Generating patient specific stem cells has been a long-standing goal in the field of regenerative medicine. These cells provide a unique platform from which to gain mechanistic insight into a variety of diseases, to carry out in vitro drug screening, to evaluate potential therapeutics and to explore gene repair coupled with cell replacement therapy. We will focus on the transcription factors and epigenetic modifications that could regulate induction of pluripotency. With a better understanding on the culture condition for reprogramming differentiated cells, we will efficiently derive human iPSCs suitable for clinical applications in the field of cardiovascular diseases.

    research1

    Embryonic stem cell colony (Debasree Dutta 2012)

    Understanding the regulation of genes implicated in physiological angiogenesis

    In a normal ovulating female, ovulation, formation of the corpus luteum, and cyclic regeneration of the endometrium, all involve physiological angiogenesis. One of the gynecological disorders associated with - (5-10% of women of reproductive age). It is characterized by the presence of endometrial epithelial and stromal cells outside the uterine cavity and new vessel formation. Endothelial cell proliferation is increased in endometrium and microvessel density is higher. Angiogenesis pertaining to implantation, placental and early embryonic development in both normal physiological and various pathological conditions forms a very important aspect of developmental biology. Placental development is perhaps the greatest physiological feat of angiogenesis occurring in the body. One of the most common medical conditions associated with post implantation in women is pre-eclampsia (diagnosed by hypertension in pregnancy and a occurrence of about 8-10% pregnant women in India) where there are substances from the placenta that can cause endothelial dysfunction in the maternal blood vessels of susceptible women. In this context, we will try to understand the regulation of few genes involved in angiogenesis that could be further used as a target for therapeutic approach.

    research2

    Endothelial network formation (Suma Seshadri G, 2014)

    Understanding the role of histone modifiers in different aspects of developmental biology

    Eukaryotic DNA is organized into chromatin in a dynamic manner that enables it to be accessed during different cellular processes. Histones, the chief protein component of chromatin, must be assembled, replaced or exchanged to preserve or change this organization according to the cellular requirements. Recent genetic studies indicate that specific histone modifications and modifying enzymes play essential roles in both global and tissue-specific chromatin organization. In particular, these studies indicate that enzymes that control levels and patterns of histone acetylation and methylation are required for normal embryo patterning, organogenesis, and survival. So we will focus on the role of these factors in different context of developmental biology

    research3

    Day 12.5 mouse embryo (Aditi Majumder, 2013)

    Extramural Grants

    • DBT (September 2012- August 2015)
    • DST (July 2013- June 2016)
    • CSIR (July 2013- June 2016)
  • Publications

    Publications

    1. Syed KM, Joseph S, Mukherjee A, Teixeira JM, Dutta D*, Pillai MR. Histone chaperone APLF regulates induction of pluripotency in murine fibroblasts. J Cell Sci. Accepted Nov. 7 2016 doi: 10.1242/jcs.194035
    2. Live detection and purification of cells based on the expression of a histone chaperone, HIRA, using a binding peptide.(2015) Kochurani KJ, Suganya AA, Nair MG, Louis JM, Majumder A, Kumar K S, Abraham P, Dutta D, Maliekal TT. Sci Rep. Nov 24;5:17218. doi: 10.1038/srep17218.
    3. Histone chaperone HIRA in regulation of transcription factor RUNX1. Majumder A, Mohieddin SK, Joseph S, Scambler PJ, Dutta D. J Biol Chem. 2015 Apr 6. pii: jbc.M114.615492.
    4. Dutta D. (2013). Signaling pathways dictating pluripotency in embryonic stem cells. Int J Dev Biol. 57(9-10):667-75
    5. Rajendran G*, Dutta D*, Hong J, Paul A, Saha B, Mahato B, Ray S, Home P, Ganguly A, Weiss ML, Paul S. (2013). Inhibition of protein kinase C signaling maintains rat embryonic stem cell pluripotency. J Biol Chem. 288(34):24351-62.
    6. Hong J, He H, Bui P, Ryba-White B, Rumi MA, Soares MJ, Dutta D, Paul S, Kawamata M, Ochiya T, Ying QL, Rajanahalli P, Weiss ML. (2013). A focused microarray for screening rat embryonic stem cell lines. Stem Cells Dev. 22(3):431-43.
    7. Home P, Saha B, Ray S, Dutta D, Gunewardena S, Yoo B, Larson M, Wolfe MW, Petrof M, Gallaghar PG, Scholz V, White KL, Golos TG, Behr B, Paul S. (2012) Altered subcellular localization of transcription factor Tead4: an evolutionary conserved mechanism to regulate first mammalian cell lineage commitment. Proc Natl Acad Science 109(19):7362-7
    8. Dutta D, Paul S. (2011). Epigenetic modifications at Vegfr1 locus in response to angiogenic signals in endothelial cells. Circulation. 124: A17031.
    9. Dutta D, Ray S, Home P, Larson M, Wolfe MW, Paul S. (2011). Self Renewal vs. Lineage Commitment of Embryonic Stem Cells: Protein Kinase C Signaling Shifts the Balance. Stem Cells. 29(4):618-28.
    10. Paul A, Samaddar N, Dutta D, Bagchi A, Chakravorty S, Chakraborty W, Gachhui R. (2011). Mercuric Ion Stabilizes Levansucrase Secreted by Acetobacter nitrogenifigens Strain RG1(T). Protein J. 30(4):262-72.
    11. Dutta D, Ray S, Home P, Saha B, Wang S, Sheibani N, Tawfik O, Cheng N, Paul S. (2010). Regulation of angiogenesis by histone chaperone HIRA-mediated incorporation of lysine 56-Acetylated histone H3.3 at chromatin domains of endothelial genes. J Biol Chem. 285:41567-77.
    12. Ray S, Dutta D, Rumi MA, Kent LN, Soares MJ, Paul S. (2009) Context- dependent function of regulatory elements and a switch in chromatin occupancy between GATA3 and GATA2 regulate Gata2 transcription during trophoblast differentiation. J Biol. Chem. 284:4977-88.
    13. Home P, Ray S., Dutta D, Larson M, Illya B, Paul S. (2009) GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J Biol Chem 284:28729-37.
    14. Dutta D, Ray S, Vivian JL, Paul S. (2008). Activation of the VEGFR1 chromatin domain: an angiogenic signal-ETS1/HIF-2alpha regulatory axis. J Biol Chem 283:25404-13.
    15. Dutta D & Gachhui, R. (2007). Novel nitrogen-fixing and cellulose producing Gluconacetobacter kombuchae isolated from Kombucha tea. Int. J. Syst. Evol. Microbiol. 57:353-357.
    16. Dutta D & Gachhui, R. (2006). Novel nitrogen-fixing Acetobacter nitrogenifigens sp. nov. isolated from Kombucha tea, Int. J. of Syst. Evol. Microbiol. 56:1899- 1903.
  • Team


    Syed Khaja Mohieddin, PhD student (DBT fellow)

    I will be focussing on the role of different histone modifiers involved in induction of pluripotency from terminally differentiated cells and how they can be manipulated to enhance the efficiency and quality of iPSC generation.

    Syed Khaja Mohieddin
    Syed Khaja Mohieddin

    Syed Khaja Mohieddin, PhD student (DBT fellow)

    I will be focussing on the role of different histone modifiers involved in induction of pluripotency from terminally differentiated cells and how they can be manipulated to enhance the efficiency and quality of iPSC generation.

    Aditi Majumder, Junior Research Fellow (DST grant)

    I am studying the molecular regulation of hemogenic endothelium involving different epigenetic factors. Hemogenic endothelium refers to the endothelium which has the hemogenic potential and thus can generate hematopoietic stem cells. In that context, it has recently become very important to understand the regulation of hemogenic endothelium.

    Aditi Majumder
    Aditi Majumder

    Aditi Majumder, Junior Research Fellow (DST grant)

    I am studying the molecular regulation of hemogenic endothelium involving different epigenetic factors. Hemogenic endothelium refers to the endothelium which has the hemogenic potential and thus can generate hematopoietic stem cells. In that context, it has recently become very important to understand the regulation of hemogenic endothelium.

    Sujata Gaikwad Prakash, PhD student (Inspire fellow)

    I am looking into how epigenetic modulator could regulate the proliferation vs. differentiation of leukemic cells.

    Aditi Majumder
    Aditi Majumder

    Sujata Gaikwad Prakash, PhD student (Inspire fellow)

    I am looking into how epigenetic modulator could regulate the proliferation vs. differentiation of leukemic cells.

  • Alumni