Profile

Research

Publications

Team

Alumni

Debasree Dutta, PhD

Scientist E-I

+91-471-2529597

debasreedutta@rgcb.res.in

daba
  • Profile

    • Ph.D Science (Jadavpur University)
    • M.Tech Biotechnology (Jadavpur University)
    • M.Sc Biochemistry (University of Kalyani)
    • B.Sc Chemistry (University of Calcutta)
    • March 2012-Till date, Scientist C at RajivGandhi Centre for Biotechnology, Trivandrum-14, Kerala
    • 2007 October- 2012 March, Postdoctoral Fellow at University of Kansas Medical Centre, Kansas City, USA
    • 2007 August-2007 September, Research Associate at Jadavpur University, Kolkata
    • National Women BioscientistAward2016 (Young category) from DBT, India
    • Student (Syed KhajaMoheddin) received Merit award for best student presentation, RGCB 2017
    • International travel grant from DBT for attending ISSCR 2013 at Boston, USA
    • DST Fast-track fellowship
    • American Heart Association Postdoctoral Fellowship (AHA), Midwest Affiliate, USA
    • Best poster at 8th Annual Greenwald Symposium on Reproductive Biology, USA
    • Fellow, Agricultural Research Scientist National Eligibility Test (ARS NET), Indian Council for Agricultural Research
    • Fellow, State Level Eligibility Test (SLET), University Grants Commission
    • Fellow, Council of Scientific and Industrial Research through National Eligibility Test (NET)
    • Associate member of International Society for Stem Cell Research (ISSCR)
    • Member of North American Vascular Biology Organization (NAVBO)
    • Member of Society for Developmental Biology (SDB)
    • Member of American Heart Association (AHA)
    • Life member of Indian Association of Cancer Research (IACR)
    • Life Member of Kerala Academy of Sciences, India
    • "Compositions and methods for establishing and maintaining stem cells in an undifferentiated state" US patent, Application Number: 2012/0270,313; Publication Date: 25/10/2012
  • Research

    To understand the molecular mechanisms regulating induction of pluripotency in differentiated cells:

    Embryonic fibroblasts upon transduced with transcription factors Oct4, Klf4, Sox2 and c-Myc generated induced pluripotent stem cells (iPSCs) similar in characteristics to embryonic stem cells (ESCs). Till now, they have been derived from different species and from other somatic cell populations with varied protocols. Their derivation is ethically and legally less problematic and technically more feasible. Reprogramming cells to a pluripotent state entails global epigenetic remodelling and introduces epigenetic changes, some of which are necessary for reprogramming to occur and others of which are inadvertently introduced during the process. Generating patient specific stem cells has been a long-standing goal in the field of regenerative medicine. These cells provide a unique platform from which to gain mechanistic insight into a variety of diseases, to carry out in vitro drug screening, to evaluate potential therapeutics and to explore gene repair coupled with cell replacement therapy. We will focus on the transcription factors and epigenetic modifications that could regulate induction of pluripotency. With a better understanding on the culture condition for reprogramming differentiated cells, we will efficiently derive human iPSCs suitable for clinical applications in the field of cardiovascular diseases.

    research1

    Embryonic stem cell colony (Debasree Dutta 2012)

    Epigenetic factors in breast cancer

    We are focussing in different epigenetic factors that could regulate or dictate the invasive or metastatic character of breast cancer. Tissues, human cell line followed by animal model experimental evidences are used as model to investigate upon these factors.

    research1

    Enhanced expression of a histone chaperone in breast cancer (Majumder A, et al. Mol Cancer, 2018)

    Understanding the role of histone modifiers in different aspects of developmental biology

    Eukaryotic DNA is organized into chromatin in a dynamic manner that enables it to be accessed during different cellular processes. Histones, the chief protein component of chromatin, must be assembled, replaced or exchanged to preserve or change this organization according to the cellular requirements. Recent genetic studies indicate that specific histone modifications and modifying enzymes play essential roles in both global and tissue-specific chromatin organization. In particular, these studies indicate that enzymes that control levels and patterns of histone acetylation and methylation are required for normal embryo patterning, organogenesis, and survival. So we will focus on the role of these factors in different context of developmental biology

    research3

    Day 12.5 mouse embryo (Aditi Majumder, 2013)

    States of pluripotency

    Two states of pluripotency exist for embryonic stem cells, primed and naïve. Unfortunately, the human ES cells generally exist in the primed state that makes it difficult to culture them and most importantly for their clonal expansion. So, it is very much needed to convert them or to derive them in naïve state to maximize the potential use of the hES cells for regenerative purpose. So, we are focussing on ES cells from different origins to investigate upon the molecular mechanism and the way out to develop naïve human ES cells.

    research3

         Primed                 vs.                        Naïve

    Extramural Grants

    • DBT on pluripotency, 2017-2019
    • DBT on cellular transition, 2017-2020
    • DST on HIRA in proliferation vs differentiation, 2017-2020
    • DBT, National Women Bioscientist Award on Breast cancer, 2018-2023
  • Publications

    Publications

    1. Majumder A, Syed KM, Mukherjee A, Lankadesari MB, Azeez JM, Sreeja S, Harikumar KB, Pillai MR, Dutta D. Enhanced expression of Histone chaperone APLF associate with breast cancer. Mol. Cancer. 2018 (Accepted)
    2. Dutta D. Histone chaperone in regulation of cellular metabolism dictating stem cell fate? Stem Cell Investig. 2017 Jun 6;4:50. Doi: 10.21037/sci.2017.06.01.
    3. Syed KM, Joseph S, Mukherjee A, Teixeira JM, Dutta D*, Pillai MR. Histone chaperone APLF regulates induction of pluripotency in murine fibroblasts. J Cell Sci. Accepted Nov. 7 2016 doi: 10.1242/jcs.194035
    4. Live detection and purification of cells based on the expression of a histone chaperone, HIRA, using a binding peptide.(2015) Kochurani KJ, Suganya AA, Nair MG, Louis JM, Majumder A, Kumar K S, Abraham P, Dutta D, Maliekal TT. Sci Rep. Nov 24;5:17218. doi: 10.1038/srep17218.
    5. Histone chaperone HIRA in regulation of transcription factor RUNX1. Majumder A, Mohieddin SK, Joseph S, Scambler PJ, Dutta D. J Biol Chem. 2015 Apr 6. pii: jbc.M114.615492.
    6. Dutta D. (2013). Signaling pathways dictating pluripotency in embryonic stem cells. Int J Dev Biol. 57(9-10):667-75
    7. Rajendran G*, Dutta D*, Hong J, Paul A, Saha B, Mahato B, Ray S, Home P, Ganguly A, Weiss ML, Paul S. (2013). Inhibition of protein kinase C signaling maintains rat embryonic stem cell pluripotency. J Biol Chem. 288(34):24351-62.
    8. Hong J, He H, Bui P, Ryba-White B, Rumi MA, Soares MJ, Dutta D, Paul S, Kawamata M, Ochiya T, Ying QL, Rajanahalli P, Weiss ML. (2013). A focused microarray for screening rat embryonic stem cell lines. Stem Cells Dev. 22(3):431-43.
    9. Home P, Saha B, Ray S, Dutta D, Gunewardena S, Yoo B, Larson M, Wolfe MW, Petrof M, Gallaghar PG, Scholz V, White KL, Golos TG, Behr B, Paul S. (2012) Altered subcellular localization of transcription factor Tead4: an evolutionary conserved mechanism to regulate first mammalian cell lineage commitment. Proc Natl Acad Science 109(19):7362-7
    10. Dutta D, Paul S. (2011). Epigenetic modifications at Vegfr1 locus in response to angiogenic signals in endothelial cells. Circulation. 124: A17031.
    11. Dutta D, Ray S, Home P, Larson M, Wolfe MW, Paul S. (2011). Self Renewal vs. Lineage Commitment of Embryonic Stem Cells: Protein Kinase C Signaling Shifts the Balance. Stem Cells. 29(4):618-28.
    12. Paul A, Samaddar N, Dutta D, Bagchi A, Chakravorty S, Chakraborty W, Gachhui R. (2011). Mercuric Ion Stabilizes Levansucrase Secreted by Acetobacter nitrogenifigens Strain RG1(T). Protein J. 30(4):262-72.
    13. Dutta D, Ray S, Home P, Saha B, Wang S, Sheibani N, Tawfik O, Cheng N, Paul S. (2010). Regulation of angiogenesis by histone chaperone HIRA-mediated incorporation of lysine 56-Acetylated histone H3.3 at chromatin domains of endothelial genes. J Biol Chem. 285:41567-77.
    14. Ray S, Dutta D, Rumi MA, Kent LN, Soares MJ, Paul S. (2009) Context- dependent function of regulatory elements and a switch in chromatin occupancy between GATA3 and GATA2 regulate Gata2 transcription during trophoblast differentiation. J Biol. Chem. 284:4977-88.
    15. Home P, Ray S., Dutta D, Larson M, Illya B, Paul S. (2009) GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J Biol Chem 284:28729-37.
    16. Dutta D, Ray S, Vivian JL, Paul S. (2008). Activation of the VEGFR1 chromatin domain: an angiogenic signal-ETS1/HIF-2alpha regulatory axis. J Biol Chem 283:25404-13.
    17. Dutta D & Gachhui, R. (2007). Novel nitrogen-fixing and cellulose producing Gluconacetobacter kombuchae isolated from Kombucha tea. Int. J. Syst. Evol. Microbiol. 57:353-357.
    18. Dutta D & Gachhui, R. (2006). Novel nitrogen-fixing Acetobacter nitrogenifigens sp. nov. isolated from Kombucha tea, Int. J. of Syst. Evol. Microbiol. 56:1899- 1903.

    Book Chapter

    Histone Chaperones Regulate Mammalian Gene Expression by Debasree Dutta, KhajaMohieddin Syed and Ananda Mukherjee in the book "Gene Expression and Regulation in Mammalian Cells - Transcription Toward the Establishment of Novel Therapeutics" edited by Fumiaki Uchiumi, ISBN 978-953-51-3868-6, Print ISBN 978-953-51-3867-9, InTech, February 2, 2018

  • Team


    Syed Khaja Mohieddin, PhD student (DBT fellow)

    I will be focussing on the role of different histone modifiers involved in induction of pluripotency from terminally differentiated cells and how they can be manipulated to enhance the efficiency and quality of iPSC generation.

    Syed Khaja Mohieddin
    Syed Khaja Mohieddin

    Syed Khaja Mohieddin, PhD student (DBT fellow)

    I will be focussing on the role of different histone modifiers involved in induction of pluripotency from terminally differentiated cells and how they can be manipulated to enhance the efficiency and quality of iPSC generation.

    Aditi Majumder, Junior Research Fellow (DST grant)

    I am studying the molecular regulation of hemogenic endothelium involving different epigenetic factors. Hemogenic endothelium refers to the endothelium which has the hemogenic potential and thus can generate hematopoietic stem cells. In that context, it has recently become very important to understand the regulation of hemogenic endothelium.

    Aditi Majumder
    Aditi Majumder

    Aditi Majumder, Junior Research Fellow (DST grant)

    I am studying the molecular regulation of hemogenic endothelium involving different epigenetic factors. Hemogenic endothelium refers to the endothelium which has the hemogenic potential and thus can generate hematopoietic stem cells. In that context, it has recently become very important to understand the regulation of hemogenic endothelium.

    Pallavi Chinnu Verghese, PhD student (Inspire fellow)

    Different cellular transitions are key to the early mammalian development. I would like to focus on different epigenetic modifications in these cellular transitions involved in pre-implantation embryo development as well as cells derived from trophectoderm and inner cell .

    pallavichinnu
    pallavichinnu

    Pallavi Chinnu Verghese, PhD student (Inspire fellow)

    Different cellular transitions are key to the early mammalian development. I would like to focus on different epigenetic modifications in these cellular transitions involved in pre-implantation embryo development as well as cells derived from trophectoderm and inner cell .

    Ishita Baral, PhD student (Institute fellow)

    My work will be to check the role of atypical protein kinase C inhibitor in the maintenance of pluripotency in embryonic stem cells and to find out if pkci has the potential in conversion of states of pluripotency. Also to find out the possible pathway crosstalks in which inhibition of PKC is involved which may results in chromatin remodelling and genome stability in embryonic stem cells of different origins.

    pallavichinnu
    pallavichinnu

    Ishita Baral, PhD student (Institute fellow)

    My work will be to check the role of atypical protein kinase C inhibitor in the maintenance of pluripotency in embryonic stem cells and to find out if pkci has the potential in conversion of states of pluripotency. Also to find out the possible pathway crosstalks in which inhibition of PKC is involved which may results in chromatin remodelling and genome stability in embryonic stem cells of different origins.

    Arya T Dharan, DST JRF

    My aim is to find out the role of histone chaperone HIRA in regulation of proliferation and differentiation inLeukemia.

    ARYATDHARAN
    ARYATDHARAN

    Arya T Dharan, DST JRF

    My aim is to find out the role of histone chaperone HIRA in regulation of proliferation and differentiation inLeukemia.

    Ashley MB, DBT JRF

    Embryonic stem cells are pluripotent cells having the property of self-renewal and proliferation in addition to its ability to give rise to cell lineages. This property can be harnessed in the field of regenerative medicine. I am working on determining the effect of an inhibitor of Protein Kinase C signalling pathway, in the maintenance of embryonic stem cells pluripotency. The effect will be compared with the other known pathways implicated to maintain the state of pluripotency.

    Ashley
    Ashley

    Ashley MB, DBT JRF

    Embryonic stem cells are pluripotent cells having the property of self-renewal and proliferation in addition to its ability to give rise to cell lineages. This property can be harnessed in the field of regenerative medicine. I am working on determining the effect of an inhibitor of Protein Kinase C signalling pathway, in the maintenance of embryonic stem cells pluripotency. The effect will be compared with the other known pathways implicated to maintain the state of pluripotency.

    Bindhu MS, Technical Assistant

    I take care of the day to day technical works related to different grants, stock inventory. I am also involved in the preparation of reagents of the lab including buffer preparation, media preparation etc. Simultaneously, also help other students and fellows in their research work.

    Bindhu
    Bindhu

    Bindhu MS, Technical Assistant

    I take care of the day to day technical works related to different grants, stock inventory. I am also involved in the preparation of reagents of the lab including buffer preparation, media preparation etc. Simultaneously, also help other students and fellows in their research work.

  • Alumni