Profile

Research

Publications

Team

Alumni

Harikumar K B, PhD

Scientist C & DBT Ramalingaswami Fellow

+91-471-2529-596

harikumar@rgcb.res.in

harikumar
harikumar

Harikumar K B, PhD

Scientist C & DBT Ramalingaswami Fellow

+91-471-2529-596

harikumar@rgcb.res.in

  • Profile

    • Ph.D Biochemistry, Amala Cancer Research Centre, Thrissur affiliated to Mahatma Gandhi University, Kottayam (2008)
    • M.Sc Biochemistry, Nagpur University, Nagpur (1999-2001)
    • B.Sc Biochemistry, Hislop College, Nagpur (1996-1999)
    • March 2012-till date, Scientist C, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram
    • July 2008- March 2012, Post Doctoral Associate, Dept. Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, USA
    • April 2007-July 2008, Research Intern, Dept. Experimental Therapeutics, M.D Anderson Cancer Center Houston, USA
    • January 2004-March 2007, Senior research fellow, Amala Cancer Research Centre, Thrissur
    • November 2001-December 2003, Junior research fellow, Amala Cancer Research Centre, Thrissur
    • International Brain Research Organization (IBRO)'s North American Laboratory activity program at UT. M. D. Anderson Cancer Center, Houston, TX in October 2015 (3 weeks).
    • Travel award from centre for International co-operation in Sciences (CICS), 2014.
    • DST Fast track fellowship (2013)
    • DBT Ramalingaswami Fellowship (2012)
    • Travel award from American Society for Biochemistry and Molecular Biology (ASBMB) Annual meeting April, 2011, Washington D.C
    • Senior Research Fellowship from Indian Council of Medical Research (ICMR), New Delhi
    • European Federation of Biotechnology
    • American Association of Indian Scientists in Cancer Research
    • Indian Association for Cancer Research
    • American Association for the Advancement of Science
  • Research

    Role of inflammation in carcinogenesis and tumor progression

    Inflammation is one of the major risk factor for the development of cancer. This project will evaluate the role of inflammation in cancer development, promotion and progression. We will be using different kinds of inflammation associated pre-clinical cancer models in rodents as our model system. We are also interested in the role of tumor associated macrophages (TAMs) in tumor progression and influence of extracellular signals on TAM function and polarity as well as molecular determinants of TAM functions.

    Role of sphingolipids in innate immune response and pathogen induced inflammation

    Sphingolipids are a class of bioactive lipid mediators characterized by the presence of a serine head group with one or two fatty acid tails. Sphingosine, one of the major sphingolipids, and its phosphorylated product Sphingosine -1-phosphate (S1P), have emerged as the modulators of multiple cellular processes, such as cell growth, survival, differentiation, angiogenesis and T cell egress, and have therapeutic potential. Innate immunity is the first line defence mechanism by our body against invading pathogens. The initial sensing of viral nucleic acid is mediated by pattern recognition receptors (PRRs), which include transmembrane bound Toll-like receptors (TLRs) and cytoplasmic proteins such as Retinoic acid inducible gene-I-like receptors (RLRs). The role of S1P system in host defence mechanism is an active area of research. In this project we are trying to identify the link between sphingolipid system and host defensive strategy against virus which will help us to understand the diverse cause-effect relationship under specific pathogenic conditions.

    • Ramalingaswami Re-entry Fellowship, Department of Biotechnology, 2012-2017
    • Fast track grant, Department of Science and Technology, 2013-2016
    • Basic research in Modern Biology task force, Department of Biotechnology, 2014-2017
  • Publications

    Peer reviewed Publications (Selected in last 5 years)

    1. Harikumar KB, Hardman R, Aranjani JM, Vimala Raveendran V, Thejass P. Immunomodulatory activity of complementary and alternative medicines. Evid Based Complement Alternat Med. 2014;2014:765107 (Editorial)
    2. Oskeritzian CA, Hait NC, Wedman P, Chumanevich A, Kolawole EM, Price MM, Falanga YT, Harikumar KB, Ryan JJ, Milstien S, Sabbadini R, Spiegel S. The sphingosine-1-phosphate/sphingosine-1-phosphate receptor 2 axis regulates early airway T-cell infiltration in murine mast cell-dependent acute allergic responses. J Allergy Clin Immunol. 2015; 135(4):1008-18
    3. Harikumar KB, Yester JW, Surace MJ, Oyeniran C, Price MM, Huang WC, Hait NC,Allegood JC, Yamada A, Kong X, Lazear HM, Bhardwaj R, Takabe K, Diamond MS, Luo C, Milstien S, Spiegel S, Kordula T. K63-linked polyubiquitination of transcription factor IRF1 is essential for IL-1-induced production of chemokines CXCL10 and CCL5. Nature Immunology, 2014 ;15(3):231-8.
    4. Liang J, Nagahashi M, Kim EY, Harikumar KB, Yamada A, Huang WC, Hait NC, Allegood JC, Price MM, Avni D, Takabe K, Kordula T, Milstien S, Spiegel S. Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation, and development of colitis-associated cancer. Cancer Cell, 2013;23(1):107-20.
    5. Price MM, Oskeritzian CA, Falanga YT, Harikumar KB, Allegood JC, Alvarez SE, Conrad D, Ryan JJ, Milstien S, Spiegel S. A specific sphingosine kinase 1 inhibitor attenuates airway hyperresponsiveness and inflammation in a mast cell-dependent murine model of allergic asthma. Journal of Allergy and Clinical Immunology, 2013 ;131(2):501-11.
    6. Van DN, Roberts CF, Marion JD, Lépine S, Harikumar KB, Schreiter J, Dumur CI, Fang X, Spiegel S, Bell JK. Innate immune agonist, dsRNA, induces apoptosis in ovarian cancer cells and enhances the potency of cytotoxic chemotherapeutics. FASEB Journal, 2012 ;26(8):3188-98.
    7. Yester JW, Tizazu E, Harikumar KB, Kordula T. Extracellular and intracellular sphingosine-1-phosphate in cancer. Cancer Metastasis Review. 2011;30(3-4):577-97.
    8. Maceyka M, Harikumar KB, Milstien S, Spiegel S. Sphingosine-1-phosphate signaling and its role in disease. Trends in Cell Biology. 2012 ;22(1):50-60.
    9. Alvarez SE#, Harikumar KB#, Hait NC, Allegood J, Strub GM, Kim EY, Maceyka M, Jiang H, Luo C, Kordula T, Milstien S, Spiegel S. Sphingosine-1-phosphate is a missing cofactor for the E3 ubiquitin ligase TRAF2. Nature. 2010;465(7301):1084-8. (#equal first author contribution).
    10. Harikumar KB, Kunnumakkara AB, Ochi N, Tong Z, Deorukhkar A, Sung B, Kelland L, Jamieson S, Sutherland R, Raynham T, Charles M, Bagherzadeh A, Foxton C, Boakes A, Farooq M, Maru D, Diagaradjane P, Matsuo Y, Sinnett-Smith J, Gelovani J, Krishnan S, Aggarwal BB, Rozengurt E, Ireson CR, Guha S. A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo. Molecular Cancer Therapeutics. 2010;9(5):1136-46.
    11. Harikumar KB, Kunnumakkara AB, Sethi G, Diagaradjane P, Anand P, Pandey MK, Gelovani J, Krishnan S, Guha S, Aggarwal BB. Resveratrol, a multitargeted agent, can enhance antitumor activity of gemcitabine in vitro and in orthotopic mouse model of human pancreatic cancer. International Journal of Cancer. 2010;127(2):257-68.

    Book Chapter

    Kuttan R and Harikumar KB . Phyllanthus species: Scientific Evaluation and Medicinal Applications (Series: Traditional Herbal Medicines for Modern Times). CRC Press, USA, 2011. (http://www.crcpress.com/product/isbn/9781439821442)

  • Team


    Sabira Mohammed, PhD Scholar (ICMR-SRF)

    The role of SphK1/S1P axis in innate immune response

    The principal objective of my study is to determine the role played by SphK1/S1P axis in host defense strategy. The innate immune system provides the first line of defense against invading pathogens. This is accomplished by recognising Pathogen Associated Molecular Patterns (PAMPs) by Pattern Recognition Receptors (PRRs). Of the PRRs, TLRs are the most widely studied. The interaction between activation of TLR by PAMP and the SphK1-S1P-S1PR axis needs further investigation to recognize role of S1P in immunity.

    Sabira Mohammed
    Sabira Mohammed

    Sabira Mohammed, PhD Scholar (ICMR-SRF)

    The role of SphK1/S1P axis in innate immune response

    The principal objective of my study is to determine the role played by SphK1/S1P axis in host defense strategy. The innate immune system provides the first line of defense against invading pathogens. This is accomplished by recognising Pathogen Associated Molecular Patterns (PAMPs) by Pattern Recognition Receptors (PRRs). Of the PRRs, TLRs are the most widely studied. The interaction between activation of TLR by PAMP and the SphK1-S1P-S1PR axis needs further investigation to recognize role of S1P in immunity.

    Manendra Babu Lankadasari, PhD Scholar (UGC-SRF)

    Sphingolipid modulation: The strategy for pancreatic cancer therapy

    Pancreatic Ductal Adenocarcinoma (PDAC) is almost universally fatal. My prime objective mainly involves the understanding and targeting of S1P metabolic pathway in context with PDAC, which will provide us new insights in cancer cell progression and new therapeutic opportunities.

    Manendra Babu L
    Manendra Babu L

    Manendra Babu Lankadasari, PhD Scholar (UGC-SRF)

    Sphingolipid modulation: The strategy for pancreatic cancer therapy

    Pancreatic Ductal Adenocarcinoma (PDAC) is almost universally fatal. My prime objective mainly involves the understanding and targeting of S1P metabolic pathway in context with PDAC, which will provide us new insights in cancer cell progression and new therapeutic opportunities.

    Yadu Vijayan, PhD Scholar (CSIR-JRF)

    Yadu Vijayan
    Yadu Vijayan

    Yadu Vijayan, PhD Scholar (CSIR-JRF)

    Shirly James, Junior Research Fellow (DST-JRF)

    Spice derived phytochemicals for cancer chemoprevention

    In my study I am exploring the possible anticancer effects of spice derived phtochemicals using pre-clinical cancer models in mouse. We are also studying the effect of combination of these phytochemicals with existing standard of care in cancer

    Shirly James
    Shirly James

    Shirly James, Junior Research Fellow (DST-JRF)

    Spice derived phytochemicals for cancer chemoprevention

    In my study I am exploring the possible anticancer effects of spice derived phtochemicals using pre-clinical cancer models in mouse. We are also studying the effect of combination of these phytochemicals with existing standard of care in cancer

    Aparna J S, (DBT-JRF)

    Our laboratory is using various pre-clinical cancer models in mice. I am mostly involved in assisting other members in the laboratory with their animal studies.

    Aparna JS
    Aparna JS

    Aparna J S, (DBT-JRF)

    Our laboratory is using various pre-clinical cancer models in mice. I am mostly involved in assisting other members in the laboratory with their animal studies.

  • Alumni