Susha Kuttiyil, DBT-Research Associate
Spermatogenesis in mammals is a complex process of germ cell differentiation that is regulated by unique control points. Identification of the key regulatory proteins would augment our understanding of the basic molecular mechanism of spermatogenesis and would serve as molecular tools for the diagnosis and treatment of spermatogenesis disorder. One such protein reported to be associated with human male factor infertility is Cyclin M1 (CNNM1). Using a mouse model, we demonstrated that Cnnm1 is expressed only in undifferentiated spermatogonial cells (SPG) which includes spermatogonial stem cells (SSCs) and/or A-type spermatogonial cells compared to the differentiated SPG cells which enter meiosis. Further, CNNM1 silencing studies showed that the protein is required for maintaining stemness and self-renewal in SPG cells. My study aims to understand the transcriptional regulation of Cnnm1 expression in SPG cells in order to validate and further analyze the function of the protein.