Profile

Research

Publications

Team

Alumni

Pradeep Kumar G, PhD

Scientist G

+91-471-2529459

kumarp@rgcb.res.in

pradip
pradip

Pradeep Kumar G, PhD

Scientist G

+91-471-2529459

kumarp@rgcb.res.in

  • Profile

    • PhD: A Biophysical Study Of Sperm Membranes – 1989 (DA University)
    • MA/MSc Zoology – 1984 (University of Kerala)
    • Present – Scientist G: Rajiv Gandhi Center for Biotechnology, Trivandrum-14, Kerala
    • 2004: Scientist-F: Rajiv Gandhi Center for Biotechnology, Trivandrum-14, Kerala
    • 1999: Reader: School of Life Sciences, DA University, Indore
    • 1994: Sr. Lecturer, School of Life Sciences, DA University, Indore
    • 1989: Lecturer, School of Life Sciences, DA University, Indore
    • Dr. Subhas Mukherjee Memorial Oration Award of Association of Clinical Embryologists, India, 2016
    • Dr. TC Anand Kumar Memorial Gold Medal and Oration Award of Indian Society for the Study of Reproduction and Fertility, India, 2016
    • 2015: Labshetwar Award from Indian Society for the Study of Reproduction and Fertility (ISSRF), India
    • 2008: DBT Overseas Associateship
    • 2007: National Bioscience Award for Career Development Department of Biotechnology, New Delhi
    • 1999-2002: Visiting Scientist Fellowship National Institute of Health, Bethesda, MD to work in University of Florida
    • 1994: Recepient of an International Post-Doctoral Fellowship award The Rockefeller Foundation, New York, USA
    • 1984: Qualified in Junior Research Fellowship Examination Council of Scientific & Industrial Research, New Delhi, India
    • 1984: Qualified in National Educational Testing University Grants Commission, New Delhi, India
    • Member, American Association for Advancement of Science, New York
    • Member, American Society for Human Genetics
    • Life Member, Indian Science Congerss Association, Kolkata
    • Life Member, Society of Biological Chemists, Bangalore, India
    • Life Member, Society for Reproductive Biology and Comparative Endocrinology (SRBCE), Chennai, India
    • Life Member, Indian Society for Study of Reproduction and Fertility (ISSRF), India
    • Life Member, Indian Society of Cell Biology, India
    • PhDs currently ongoing: 7
    • PhDs awarded : 14

    Funding Agencies

    • Department of Science and Technology
    • Council of Scientific and Industrial Research
    • Department of Atomic Energy
    • Department of Biotechnology
    • The United States – Israel Binational Science Foundation (BSF)

    Journals

    • The American Journal of Obstetrics and Gynecology
    • Indian Journal of Clinical Biochemistry
    • Andrologia
    • Current Opinions in Biology
    • Journal of Membrane Biology
    • Plant Science
    • Journal of Histochemistry and Cytochemistry
    • Journal of Plant Physiology
    • Fertility and Sterility
    • Acta Biochimica Polonica
    • BBA- Gene Regulatory Mechanisms
    • Journal of Biophysics and Structural Biology
    • Journal of Biosciences
    • Journal of Reproductive Immunology
    • Indian Journal of Biotechnology
    • Activators of Cyclin-Dependent Kinases (ACDK).US and International Patent
      PCT/US02/23147
    • 2000-2002: Visiting Scientist, University of Florida, Florida
    • 1996-1996: Visiting Scientist, University of Virginia, Virginia
    • 1994-1995: Fellow-in-Residence, The Rocekfeller University, New York
  • Research

    Germline stem cell maintenance, division and differentiation in testis

    Germline stem cells (GSCs) are lineage of Primordial Germ Cells (PGCs) which may have retained the full level of pluripotency as that of the PGCs, or have lost a part of it to qualify to be a multipotent cell line. Mammalian testis is a place orchestrating one among the very fast cell division patterns in the entire body, amounting to the production of 1000 spermatozoa per heart beat in an adult human being. Despite this pace of division, germ cells manifest minimal loss of genome integrity. Our objectives include characterization of GSCs, harvesting and manipulation of GSCs to generate cells of interest and to understand mechanisms involved in GSC maintenance and differentiation.

    Factors regulating meiotic drive in testis

    Meiosis occurs in germ cells in the testis and ovary. Somatic cells are incapable of dividing through meiosis. In tesist, enormous number of cells undergo meiosis in a precisely synchronized fashion. We are addressing the molecular control of the processes associated with germ cells differentiation into spermatogonia, primary spermatocytes and ultimately into haploid secondary spermatocytes. We use differential display genomic and proteomic approaches to understand alterations in the levels of expression of a large subset of testis-specific proteins. Special focus is given to differential proteome of spermatozoa from fertile human males and subfertile human males with spermatogenic impairment. We also monitor differential proteome of primary spermatocytes and secondary spermatocytes to define meiosis-associated molecular events.

    Critical molecules regulating biomembrane structure and cell fusion during fertilization

    While it is hard to fuse two cells even from the same individual, fertilization poses a natural mechanism through which two haploid cells from two different individuals interact in a highly species-specific manner to yield a single diploid cell. During fertilization, the membrane of spermatozoon fuses with the membrane of oocyte with the highest level of specificity that one could think of. We work on the molecular aspects of cell-cell recognition, membrane-membrane interaction and the physical aspects of membrane structure that aids in fusion events. We have identified a few "critical molecules" involved in gamete interaction, and the absence of any one of these key players has been shown to have strong correlation with compromised gamete functioning. A few of these critical molecules are associated with sperm membrane RAFTS. In this context, we are evaluating the role of RAFTS and RAFT-associated proteins (RAPs) in determining the ability of spermatozoa to recognize, interact and fuse with oocytes.

    Cellular Deprogramming during fertilization

    Spermatozoa and oocyte are terminally differentiated cells with no "future" if they do not fuse. The fusion of these two cells produces a single cell, which is the storehouse of the entire pluripotency that would be required for the generation of an entire organism with extremely complex and diversified system. Oocyte cytoplasm might reprogram gene expression from male nucleus, and the spermatozoon (both its nucleus and non-nuclear components) might redefine gene expression in the oocyte. The role of sperm components in defining the division pattern in early embryos is obscure. In collaboration with Dr. Malini Laloraya, we address the intricacies of cellular deprogramming as an immediate early event during fertilization.

    1. Bhagya KP (2007-) Functional evaluation of AIRE in mammalian testis
    2. Sreesha Sree (2008-) Role of microRNAs in germ cell differentiation and fertilization
    3. Divya Saro Varghese (2008-) Functional analysis of TDP-43 in relation to spermatogenesis
    4. Nomesh Yadu (2009-) Evaluation of retinoic acid signaling pathways in germline stem cell differentiation
    5. Karthika Radhakrishnan (2011-) The role of epigenetic regulation in germ cell differentiation
    6. Soumya A (2012-) Interactions between sperm membrane rafts and zona pellucida proteins
    7. Devi AN (2013-) Expression profiling and functional characterization of Nephrocystin in relation to spermatogenesis.
    1. Singh A (1993) Role of free radicals and superoxide dismutase in the modulation of sperm membrane conformation. Devi Ahilya University, Indore.
    2. Sinha S (1994) Biochemical and biophysical studies of human spermatozoa with special reference to oligospermia. Devi Ahilya University, Indore.
    3. Singh PP (1995) Iodine activation, peripheral conversion of thyroxine to triiodothyronine and biological alterations in thyroid follicular membranes with special reference to temperature in Columba livia. Devi Ahilya University, Indore.
    4. Suvro Chatterjee (1999) Biophysical and biochemical perturbations in spermatozoa during infertility induction in males. Devi Ahilya University, Indore
    5. Sharad Purohit (2000) Distribution of surface saccharides and egg binding proteins on the surface of sperm cells of normal and oligospermic human subjects. Devi Ahilya University, Indore
    6. Deeksha Saxena (2002) Characterization of implantation specific proteins and protein encoding genes in the mouse uterus. Devi Ahilya University, Indore.
    7. Kumar Chandrakuntal (2004) Blue light signal transduction in plant coleoptile tips: involvement of active oxygen species in phototropism. Devi Ahilya Unviersity, Indore
    8. Brahma Raju M (2007) Evaluation of the role of testis expressed proteins with reference to sperm development and fertilization. Devi Ahilya University, Indore
    9. Mohammad Shoeb (2011) Identification and characterization of membrane lipid rafts in mammalian spermatozoa
    10. Ambika Chandrashekhar (2012) Molecular and functional characterization of testis expressed Nox5 in relation to sperm development
    11. Uma Chandran (2012) Identification of testis expressed cell cycle regulating molecules with special reference to meiosis
    12. Indu S (2013) Identification and functional evaluation of factors regulating testicular germ line stem cell division and differentiation

    Completed Sponsored Grants

    1. Insect pest management through secondary plant metabolites: control of forest pest and mosquitoes (Funded by MP Council of Science and Technology, Bhopal, India). Duration: 1988-1991
    2. Studies on the isolation, purification and structural and functional characterization of Fc receptors from mammalian sperm (funded by Council of Scientific and Industrial Research, New Delhi, India). Duration: 1990-1992
    3. Free radical modulation of the physico-chemical architecture of mammalian sperm membranes and the molecular aspects of sperm-zona pellucida interaction (funded by Department of Science and Techology, New Delhi, India). Duration: 1992-1995
    4. Active oxygen intermediates in female reproduction and the physico-chemical aspects of membrane configuration as a consequence of oxidative mechanisms at implantation (funded by Department of Science and Techology, New Delhi, India). Duration: 1992-1995
    5. Isolation, purification and characterization of a transiently appearing endometrial adhesion molecule (EAM) in the mouse (funded by Department of Atomic Energy, Bombay, India). Duration: 1992-1995
    6. Biophysical and immunological aspects of the perturbations in post-vasectomy spermatozoa and its relation in irreversibility of fertility (funded by Council of Scientific and Industrial Research, New Delhi). Duration: 1996-2000
    7. Characterization of A/V CAM from human spermatozoa and its involvement in normal human fertility (funded by Department of Biotechnology, New Delhi, India). Duration: 2000-2004. Charactrization of 54 kilodalton uterine protein and its involvement in early pregnancy (funded by University Grants Commission, New Delhi, India). Duration: 2002-2005
    8. Identification of superoxide generating machinery in mammalian spermatozoa (funded by Department of Science and Technology, New Delhi, India). Duration: 2003-2007.Mechanism of activation of NADPH Oxidase in blue-light signal transduction reactions in the coleoptile tip (Department of Science and Technology, New Delhi, India). 2004-2008
    9. Evaluation of the expression of a sterility locus gene TCTEX-1 and its relation with protein trafficking in spermatozoa from infertile and fertile human males (BRNS, Bombay). Duration: 2006-2009.
    10. Characterization of RAFTS and RAFT-associated proteins of spermatozoa during capacitation and acrosome reaction. (Department of Biotechnology, New Delhi). 2006-2009
    11. Cloning, expression and semiquantitative analysis of protocadherins in human germ cells (CSIR, New Delhi) 2006-2009
    12. Sperm proteome profiling in human male infertility (DBT-National Bioscience Award Project) 2007-2010
    13. Cloning, expression and functional characterization of a cyclin-like protein (CLP-1) from human germ cells (DST, New Delhi) 2008-2011
    14. Charcterization of testis-specific tyramine receptor like protein (TRLP) (KSCSTE, Trivandrum) 2008-2011
    15. Isolation of germline stem cells from adult mouse testis and evaluation of their pluripotency (Department of Biotechnology, New Delhi) 2010-2011

    Ongoing Sponsored Grants

    1. Structural and phosphoproteome analysis of mouse sperm membrane rafts during their interaction with zona pellucida protein (Department of Biotechnology, New Delhi), 2011-2014
    2. Association between stemness and TCTEX1 expression in testicular germ cells from adult mouse. (BRNS, Mumbai), 2011-2014
    3. Evaluation of cellular aging and genome stability in spermatogonial stem cells (CSIR, New Delhi), 2013-2016.
    4. Role of CLP-1 in cell cycle regulation in spermatogenic cells (DST, New Delhi), 2013-2016.
  • Publications

    1. Laloraya, M., Kumar GP and Laloraya, M.M. (1988) Changes in the levels of superoxide radical and superoxide dismutase during the estrous cycle of Rattus norvegicus and induction of superoxide dismutase by activity by lutropin. Biochem. Biophys. Res. Commun. 157(1), 146 153.
    2. Kumar GP, Laloraya, M., Agrawal, P. and Laloraya, M.M. (1989) The involvement of surface sugars of mammalian spermatozoa in epididymal maturation and in vitro sperm zona recognition. Andrologia. 22(2), 179 183.
    3. Laloraya, M., Kumar GP and Laloraya, M.M. (1989) Histochemical study of superoxide in the ovary of the rat during the oestrous cycle. J. Reprod. Fertil. 86(2), 583 587.
    4. Kumar GP, Laloraya, M. and Laloraya, M.M. (1989) Coupling of a proton pump with superoxide radical superoxide dismutase systems in mammalian spermatozoa and its association with sperm motility. Biochem. Biophys. Res. Commun. 161(2), 771 775.
    5. Laloraya, M., Kumar GP and Laloraya, M.M. (1989) A possible role of superoxide anion radical in the process of blastocyst implantation in Mus musculus. Biochem. Biophys. Res. Commun. 161(2), 762 770.
    6. Kumar GP, Laloraya, M. and Laloraya, M.M. (1989) The effect of some of the polyphenolic compounds on sperm motility in vitro: a structure activity relationship. Contraception., 39, 531 539.
    7. Kumar GP, Laloraya, M. and Laloraya, M.M. (1990) Powerful anti sperm motility action of cobaltous ion and its recovery by a sulfhydryl compound. Contraception 41, 633 639.
    8. Laloraya M, Kumar GP, Nivsarkar M and Laloraya MM (1990) Localization of superoxide dismutase in the ovary and uterus of imcce during pregnancy with special reference to steroidogenesis and implantation. Acta Histochem. et Cytochem. 23(3), 271 279.
    9. Verma S, Kumar GP, Laloraya M, Singh A, Nivsarkar M and Bharti S (1990) Discovery of a novel and alternate hydrogen peroxide generation machinery in the mammalian thyroid gland that modulates iodination of tyrosine. Biochem. Biophys. Res. Commun. 169(1), 1 7.
    10. Verma S, Kumar GP, Laloraya M and Singh A (1990) Activation of iodine into a free radical intermediate by superoxide: A physiologically significant step in the iodination of tyrosine. Biochem. Biophys. Res. Commun. 170(3), 1026 103.
    11. Kumar GP, Tillo M, Laloraya M, Nivsarkar M, Verma S and Singh A (1990) Superoxide anion radical induces lipid phase transitions that mimics temperature induced phase transition phenomena of membrane lipids. Biochem. Biophys. Res. Commun. 172(2), 601 607.
    12. Kumar GP, Seerwani N, Laloraya M, Nivsarkar M, Verma S and Singh A (1990) Superoxide dismutase as a regulatory switch in mammalian testicular steroidogenesis. Biochem. Biophys. Res. Commun. 173(1), 302 308.
    13. Sinha S, Kumar GP, Laloraya M and Warikoo D (1991) Overexpression of superoxide dismutase and lack of surface thiols in spermatozoa: Inherent defects in oligospermia. Biochem. Biophys. Res. Commun. 174(2), 510 519.
    14. Nivsarkar M, Kumar GP, Laloraya M and Laloraya MM (1991) Superoxide dismutase in the anal gills of the larvae of mosquito Aedes aegyptii : its inhibition by alpha terthienyl. Arch. Insect Biochem. Physiol. 16(4), 249 255.
    15. Kumar GP, Laloraya M and Laloraya MM (1991) Superoxide radical level and superoxide dismutase activity changes in maturing mammalian spermatozoa. Andrologia 23(2), 171 175.
    16. Laloraya M, Kumar GP and Laloraya MM (1991) Changes in the superoxide anion radical and superoxide dismutase levels in the uterus of Rattus norvegicus during the estrous cycle and a possible for superoxide radical in uterine oedema and cell proliferation at proestrous. Biochem. Cell Biol. 69, 313 316.
    17. Verma S, Kumar GP, Laloraya M, Nivsarkar M and Singh A (1991) Superoxide dismutase activation in thyroid and suppression in adrenal : Novel pituitary regulatory routes. FEBS Letters 282(2), 310 312.
    18. Singh A, Kumar GP, Laloraya M, Verma S and Nivsarkar M (1991) Superoxide activity regulation by spermine : A new dimension in spermine biochemistry and sperm development. Biochem. Biophys. Res. Commun. 177(1), 420 426.
    19. Nivsarkar M, Kumar GP, Laloraya M and Laloraya MM (1991) Superoxide dismutase inactivation by alpha terthienyl : A novel observation in thiophene photochemistry. Pesticide Biochem. Physiol. 41(1), 53 59.
    20. Kumar GP, Sinha S, Laloraya M and Nivsarkar M (1991) Enhanced axial symmetry at the Fe(III) heme centre of peroxidase by ascorbate : A basis for the ascorbate dependent peroxidase action. Biochem. Biophys. Res. Commun. 180(2), 597 601.
    21. Nivsarkar M, Kumar GP, Laloraya M and Laloraya MM (1992) Generation of superoxide anion radical by alpha terthienyl in the anal gills of mosquito larvae Aedes aegyptii : A new aspect in alpha terthienyl phototoxicity. Arch. Insect Biochem. Physiol. 19(4) 261 270.
    22. Purohit S, Kumar GP, Laloraya M, Bharti S and Laloraya MM (1992) ABA induced lipid melting and its reversal by umbelliferone in the plasmalemma of the guard cell protoplast : A breakthrough in plant hormone receptor binding and hormone action. Biochem. Biophys. Res. Commun. 182(2), 652 658.
    23. Nivsarkar M, Kumar GP, Laloraya M and Laloraya MM (1993) Effect of ultraviolet activated alpha terthienyl upon anal gill membranes of mosquito larvae : A spin label study. Biochem. Syst. Ecol. 21(4), 441 447.
    24. Kumar GP (1993) Lipid phase transitions and a possible lipid protein lattice structure as an activity regulator in maturing spermatozoa. Biochem. Mol. Biol. Int. 29(6), 1029 1038.
    25. Jain S, Thomas M, Kumar GP and Laloraya M (1993) Programmed lipid peroxidation of biomembranes generating kinked phospholipids permitting local molecular mobility : A peroxidative theory of fluidity management. Biochem. Biophys. Res. Commun. 195(2), 574 580.
    26. Sinha S., Kumar GP and Laloraya M (1993) Methyl xanthines and altered biomembrane dynamics: Demonstration of protein mobility and enzyme inhiibition by caffeine in sperm model system. Biochem. Mol. Biol. Int. 31(6), 1141 1148.
    27. Chatterjee S, Laloraya M and Kumar GP (1993) Exorbitantly enhanced protein gyration and erroneous membrane modification programs in spermatozoa after vasectomy: A biophysical basis for low fertility revival after vasectomy. Biochem. Biophys. Res. Commun. 197(2), 450 456.
    28. Sinha S, Kumar GP and Laloraya M (1993) The topography of thiol distribution on sperm surface: A sensitive marker for human fertility diagnosis. Clinica Chimica Acta. 225, 83 84.
    29. Sinha S, Kumar GP and Malini Laloraya (1994) Abnormal physical architecture of the lipophilic domains of human sperm membrane in oligospermia: a logical cause for low fertility profiles. Biochem. Biophys. Res. Commun. 198(1), 266 273.
    30. Thomas M, Kumar GP and Laloraya M (1994) Transient thiol expression and thiol disulphide cluster receptor communications a molecular basis for blastocyst uterine interactions. Biochem. Biophys. Res. Commun. 198(1), 150 157.
    31. Nivsarkar M, Kumar GP and Laloraya M (1994) Photophyscial alterations of erythrocyte membrane by 4,5′,8 trimethyl psoralen : Membrane response towards psoralen phototherapy. Biochem. Biophys. Res. Commun. 199(3), 1413-1421.
    32. Chatterjee S, Laloraya M and Kumar GP (1994) Free radical bombing of soermatozoa in spermatic granuloma: an attempt to prevent autoimmune switch on. Biochem. Biophys. Res. Commun. 201(1), 472 477.
    33. Laloraya, M.M., Kumar GP and Laloraya, M. (1994) Photochemical reaction sequences of blue light acivated flavins: sensory transduction through free radical messengers. Biochem. Mol. Biol. Intl. 33(3), 543 551.
    34. Jain, S, Thomas, M., Kumar GP & Laloraya, M. (1994) Appearence of homogenious smectic multilamellar microenvironments in biomembranes undergoing superoxide initiated lipid peroxidation: lipid dienyl radical accumulation and fludity management in lipid bilayers. Biochem. Mol. Biol. Intl. 33(5), 853-862.
    35. Purohit S, Kumar GP, Laloraya M amd Laloraya MM (1994) Involvement of superoxide radical in signal transduction regulating stomatal movements. Biochem. Biophys. Res. Commun. 205(1), 30-37.
    36. Hajela K, kayestha R and Kumar GP (1994) Carbohydrate-induced modulation of cell membrane. 1. Interaction of sialic acid with peripheral blood lymphocytes: a spin label study. FEBS Lett. 354, 217-219.
    37. Thomas M, Jain S, Kumar GP and Laloraya M (1995) Variation in reactive sulphydryl distribution in mouse uterus in early pregnancy. Acta Histochem. Cytochem. 28(3), 227-230.
    38. Laloraya M, Kopergaokar S, Jain S, Thomas M and Kumar GP (1996) Estrogen surge: a regulatory switch for superoxide radical generation at implantation. Biochem. Mol. Biol. Int. 39(5), 933-940.
    39. Nivsarkar M, Kumar GP and Laloraya M (1996) Metal binding and resultant loss of phototoxicity of alpha-terthienyl: metal detoxification versus alpha-terthienyl inactivation. Bull. Environ. Contam. Toxicol. 56, 183-189.
    40. Kumar GP, Laloraya M and Koide SS (1996) Characterization of a uterine luminal fluid protein ULF-250 using N-terminal microsequencing and RT-PCR identify a novel estrogen-regulated gene in the rat uterus. FEBS Letters 399, 33-36.
    41. Chatterjee S, Laloraya M and Kumar GP (1997) Free radical induced liquefaction of ejaculated human semen: A new dimension in semen biochemistry. Arch. Androl. 38, 107-111.
    42. Negishi H, Kumar GP, Laloraya M and Koide SS (1997) Neutralization of pregnancy preventing activity of cabergoline, a dopamine agonist, with progesterone. Theriogenology 48, 241-246.
    43. Thomas M, Jain S, Kumar GP and Laloraya M (1997) Superoxide causes hatching of mouse blastocysts in vitro and a possibility of blastocyst generated superoxide causing hatching in vivo. J. Cell Sci. 110, 1597-1602.
    44. Laloraya M, Thomas M, Jain S, Jafri F and Kumar GP (1997) Activity profile of placental superoxide-superoxide dismutase system in pregnant mice and its possible relation with placental steroidogenesis. Biochem. Mol. Biol. Int. 42(5), 983-990.
    45. Chatterjee S, Purohit SB, Laloraya M and Kumar GP (1997) Vasectomy-induced SOD inactivation in the male reproductive tract of rat : A prerequisite for spermatic granuloma formation. Urologia Internationalis 645, 59 (1), 23-25.
    46. Chatterjee S, Laloraya M and Kumar GP (1997) Exessively exposed surface thiol of mouse spermatozoa after vasectomy with special reference to free radical membrane perturbation : A possible cause of postvasectomy infertility. Arch. Androl. 000, 00-00.
    47. Chatterjee S, Laloraya M and Kumar GP (1997) Superoxide anion radical mediated dissection of rat sperm. An in vitro study. Arch. Androl. 39, 101-104.
    48. Chatterjee S, Reddy LK, Laloraya M and Kumar GP (1997) Free radical mediated sperm load managment in the vagina of rat. Arch. Androl. 39, 127-133.
    49. Prakash P, Kumar GP, Laloraya M, Javeri T and Parihar MS (1997) Superoxide anion radical production as a cadmium-mediated mechanism of toxicity in avian thyroid: an electron spin resonance study by spin trapping. Comp. Biochem. Physiol. 118C(1), 89-95.
    50. Singh PP, Kumar GP and Laloraya M (1997) Regulation of superoxide anion radical-superroxide dismutase system in the avian thyroid by TSH with reference to thyroid hormonogenesis. Biochem. Biophys. Res. Commun. 239 (1), 212-216.
    51. Kumar GP and Laloraya M (1997) Contraception In: Oxidative stress in male infertility (Ochsendorf FR and Fuchs J, eds.), Gardez! Verlag, St. Augustin, Germany, pp. 185-196.
    52. Prakash P, Laloraya M and Kumar GP (1998) Starvation-induced hypothyroidism involves perturbations in thyroid superoxide-SOD system in pigeons. Biochem. Mol. Biol. Int. 45(1), 73-83.
    53. Purohit SB, Laloraya M and Kumar GP (1998) Acrosome reaction inducers impose alterations in repulsive strain and hydration barrier in human sperm membranes. Biochem. Mol. Biol. Intl. 45(2), 227-235.
    54. Prakash P, Kumar GP, Laloraya M, Hemnani T and Parihar MS (1998) Superoxide anion radical generation as a temperature stress response in the gills of fresh water catfish Heteropneustes fossilis: role in mucus exudation under elevated temperature. Comp. Biochem. Physiol. C. Pharmacol. Toxicol. Endocrinol. 119(2), 211-216.
    55. Purohit SB, Laloraya M and Kumar GP (1998) Bicarbonate-dependent lipid ordering and protein aggregation are part of non-genomic action of progesterone on capacitated spermatozoa. J. Androl. 19(5), 608-18.
    56. Chopra RK, Dalal M, Kumar GP and Laloraya M (1998) A genetic system involving superoxide causes F-1 necrosis in wheat (T. aestivum L.) Biochem. Biophys. Res. Commun. 248(3), 712-715.
    57. Prakash P, Laloraya M and Kumar GP (1998) Influence of a melatonin implant on the free radical load in avian thyroid and its relation with thyroid hormonogenesis. Biochem. Mol. Biol. Intl. 46(6), 1249-1258.
    58. Purohit SB, Laloraya M and Kumar GP (1999) Role of ions and ion-channels in capacitation and acrosome reaction of spermatozoa. Asian J. Androl. 1(3), 95-107.
    59. Jain S, Saxena D, Kumar GP and Koide SS and Laloraya M (1999) Effect of estradiol and selected antiestrogens onn pro- and anti-oxidant pathways in mammalian uterus. Contraception 60, 111-118.
    60. Agnihotri S, Purohit SB, Laloraya M and Kumar GP (1999) Regional heterogeneity in intracellular distribution of superoxide and hydrogen peroxide within the sperm and its relation to sperm development Arch. Androl. 43, 113-121.
    61. Laloraya MM, Kumar CK, Kumar GP Laloraya M and (1999) Active oxygen species in blue light mediated signal transduction in coleoptile tips. Biochem. Biophys. Res. Commun. 256(2), 293-298.
    62. Jain S, Saxena D, Kumar GP and Laloraya M (2000) NADPH dependent superoxide generation in the ovary and uterus of mice during estrus cycle and early pregnancy. Life Sci. 66, 1139-1146.
    63. Purohit SB, Saxena D, Laloraya M and Kumar GP (2000) Altered molecular dynamics and antioxidant status in the spermatozoa in a testosterone induced oligozoospermia model. Mol Reprod. Dev. 55(3), 316-325.
    64. Saxena D, Purohit S.B., Kumar GP and Laloraya M. (2000) Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation. Nitric Oxide: Biology and Chemistry 4(4), 384-391.
    65. Chatterjee, S. Laloraya, M. and Kumar GP (2001) Regional Variations in Thiol Distribution Pattern and Superoxide Dismutase Activity of the Male Reproductive Tract of the Rat Modulate the Transport of Spermatozoa through the Epididymis and Vas deferens. Urologia Int. 66(2), 100-104.
    66. Kumar GP, Malini Laloraya, Cong-Yi Wang, Quin-Guo Ruan, Abdoreza Davoodi-Semiromi, Kuo-Jang Kao, and Jing-Xiong She (2001) The Autoimmune Regulator (AIRE) Is a DNA-binding Protein J. Biol. Chem., Vol. 276, Issue 44, 41357-41364.
    67. Chatterjee S, Rahman MM, Laloraya M, Kumar GP (2001) Sperm disposal system in spermatic granuloma: a link with superoxide radicals. Int. J Androl. 24(5):278-83.
    68. Kumar GP, Laloraya M and She JX (2002) Population genetics and functions of the autoimmune regulator (AIRE) Endocrinol. Metabol. Clinics of North America. 31(2), 321-338
    69. Wang CY, Shi JD, Yang P, Kumar GP, Li QZ, Run QG, Su YC, Scott HS, Kao KJ, She JX. (2003) Molecular cloning and characterization of a novel gene family of four ancient conserved domain proteins (ACDP). Gene. 306, 37-44.
    70. Davoodi-Semiromi R, Laloraya M, Kumar GP, Purohit SB, Jha RK and She JX (2004) A mutant Stat5b with weaker DNA binding affinity defines a key defective pathway in non-obese diabetic (NOD) mice. J. Biol. Chem. 279(12):11553-61.
    71. Shoeb M, Laloraya M and Kumar GP (2004) Formation and dynamic alterations of horizontal microdomains in sperm membranes during progesterone-induced acrosome reaction. Biochem. Biophys. Res. Commun. 315(3), 763-770.
    72. Purohit SB, Brahmaraju M, Palta A, Shukla S, Laloraya M and Kumar GP (2004) Impaired E-cadherin expression in human spermatozoa in a male factor infertility subset signifies E-cadherin-mediated adhesion mechanisms operative in sperm-oolemma interactions. Biochem. Biophys. Res. Commun. 316(3), 903-909.
    73. Nautiyal J, Laloraya M and Kumar GP (2004) 17ß-estradiol induces nuclear translocation of Crk-L at the window of embryo implantation. Biochem. Biophys. Res. Commun. 318(1):103-12.
    74. Brahmaraju M, Shoeb M, Laloraya M and Kumar GP (2004) Spatio-temporal organization of Vam6P and SNAP on mouse spermatozoa and their involvement in sperm-zona pellucida interactions. Biochem. Biophys. Res. Commun. 318(1):148-55.
    75. Chandrakuntal K, Kumar GP, Laloraya M, Laloraya MM (2004) Direct involvement of hydrogen peroxide in curvature of wheat coleoptile in blue-light treated and dark-grown coleoptiles. Biochem. Biophys. Res. Commun. 319, 1190-1196.
    76. Nautiyal J, Kumar GP and Laloraya M (2004) Mifepristone (Ru486) antagonizes Monocyte Chemotactic Protein-3 downregulation at early mouse pregnancy revealing immunomodulatory events in Ru486 induced abortion. Am. J. Reprod. Immunol. 51, 1-12.
    77. Purohit SB, Kumar GP, Laloraya M and She JX (2005) Mapping the DNA-binding and dimerization domains of the autoimmune regulator (AIRE) protein using deletion constructs. Biochem. Biophys. Res. Commun. 327, 939-944.
    78. Shukla S, Jha RK, Laloraya M and Kumar GP (2005) Identification of non-mitochondrial NADPH oxidase and the spatio-temporal organization of its components in mouse spermatozoa. Biochem. Biophys. Res. Commun. 331(2), 476-483.
    79. Laloraya M, Davoodi-Semiromi A, Kumar GP, McDuffie M, and She J-X (2006) Impaired Crkl expression contributes to the defective DNA binding of Stat5b in Non-Obese Diabetic (NOD) Mice. DIABETES 55 (3), 734-741.
    80. Chandrakuntal K, Thomas NM, Kumar G.P., Laloraya M and Laloraya MM (2006) Fluorescence resonance energy transfer between polyphenolic compounds and riboflavin indicates a possible accessory photoreceptor function for some polyphenolic compounds. Photochem. Photobiol. 82(5), 1358-1364.
    81. ha RK, Titus, S, Saxena D, Laloraya M and Kumar GP (2006) Profiling of E-cadherin, beta-catenin and calcium in embryo-uterine interactions at implantation. FEBS Lett. 580(24), 5653-5660.
    82. Selot R, Kumar V, Shukla S, Chandrakuntal K, Brahmaraju M, Dandin SB, Laloraya M and Kumar PG (2006) Identification of a soluble NADPH oxidoreductase (BmNOX) with antiviral Activites in the gut juice of Bombyx mori. Biosci. Biotechnol. Biochem. 71(1), 200-205.
    83. Chandran U, Laloraya M and Kumar PG (2007) Identification of testis-expressed cell cycle regulating proteins with special reference to meiosis. J. Endocrinol. Reprod. 11(1), 45-48.
    84. Mohan JJ, Jha RK, Kumar PG , Dharmarajan AM and Laloraya M (2007) Secreted Frizzle-Related Protein (sFRP4) can abrogate pregnancy - a new dimension in its biological role J. Endocrinol. Reprod. 11(1), 41-44.
    85. Purohit S, Laloraya M and Kumar PG (2008) Distribution of N- and O-linked oligosaccharides on surface of spermatozoa from normal and infertile subjects. Andrologia 39, 1-6.
    86. Brahmaraju M, Laloraya M and Kumar PG (2009) Immunodominant sperm antigens and their role in male infertility. In: Current Paradigm of Reproductive Immunology, 2009: 59-81.
    87. Majumder S, Rajaram M, Muley A, Reddy HS, Tamilarasan KP, Kolluru GK, Sinha S, Siamwala JH, Gupta R, Ilavarasan R, Venkataraman S, Sivakumar KC, Anishetty S, Kumar PG and Chatterjee S (2009) Thalidomide attenuates nitric oxide driven angiogenesis by interplaying with soluble guanylate cyclase. Br. J. Pharmacol. 158, 1720-1734.
    88. Selot R, Kumar VK, Sekhar SC and Kumar PG (2010) Molecular characterization and expression analysis of BmNOX in two strains of Bombyx mori with contrasting viral resistance phenotype. Arch. Insect Biochem Physiol. 73(3), 163-175.
    89. Chandrakuntal K, Shah AK, Thomas NM, Karthika V, Laloaya M, Kumar PG and Laloraya MM (2010) Blue light exposure targets NADPH oxidase to plasma membrane and nucleus in wheat coleoptiles. J Plant Growth Reg 29:232-241.
    90. Shoeb M, Laloraya Mand Kumar PG (2010)Progesterone-induced reorganization of NOX-2 components in membrane rafts iscritical for sperm functioning in Caprahircus. Andrologia 42, 356-365.
    91. Kumar PG and Shoeb M (2011) The role of Trp ion channels in testicular function. Adv. Exp. Med.Biol. 704, 881-908.
    92. Chandrasekhar Aand Kumar PG (2011) Modulation of NOX2 activity through sequentialpost-translational modifications of p22phox during capacitation and acrosomereaction in goat spermatozoa. J. Animal Sci. (doi: 10.2527/jas.2010-3731)
    93. Brahmaraju M, Bhagya KP, Titus S, Sebastian A, Devi AN, Laloraya M and Kumar PG (2011) AIRE1a mightbe involved in cyclin b2 degradation inoocytes and testicular lysates. Biochem. Cell Biol. 89(4), 411-422.
    94. Radha A, Sreesha S, Faisal K, Kumar PG, Oommen OV, Akbarsha MA (2014) Antigenic homogeneity of male Müllerian gland (MG) secretory proteins of a caecilian amphibian with secretory proteins of the mammalian prostate gland and seminal vesicles: evidence for role of the caecilian MG as a male accessory reproductive gland. Zoology (DOI: 10.1016/j.zool.2014.03.005)
    95. Sreesha S, Radhakrishnan K, Indu S and Kumar PG (2014) Dramatic changes in 67 microRNAs during Initiation of first wave of spermatogenesis in Mus musculus testis: Global regulatory insights generated by microRNA-mRNA network analysis. Biol. Reprod. 91 (3) 69, 1-11. (doi: 10.1095/biolreprod.114.119305).
    96. Devi AN, Kumar ATR, Pillai SM, Jayakrishnan K and Kumar PG (2015) Expression profiles of NPHP1 in the germ cells in the semen of men with male factor infertility. Andrology 3, 685-693 (DOI: 10.1111/andr12062).
    97. Indu S, Sekhar SC, Jeeva S, Anilkumar TR, Pillai SM, Laloraya M and Kumar PG (2015) Aberrant expression of DYNLT1 is associated with human male factor infertility. Mol Cell Proteom 14(12):3185-95 (doi:10.1074/mcp.M115.050005).
    98. Varghese DS, Chandran U, Soumya A, Pillai SM, Jayakrishnan K, Reddi PP and Kumar PG (2016) Aberrant expression of TAR DNA binding protein-43 is associated with spermatogenic disorders in human males. Reprod. Fertil. Dev. 28(6):713-22 (DOI: 10.1071/RD14090).
    99. Chandran U, Indu S, Anilkumar TR, Devi AN, Khan I, Srivastava D and Kumar PG (2016) Expression of Cnnm1 and its association with stemness, cell cycle and differentiation in spermatogenic cells in mouse testis. Biol. Reprod (doi:10.1095/biolreprod.115.130369)
    100. Radhakrishnan K, Bhagya KP, Anilumar TR, Devi AN, Sengottaiyan J and Kumar PG (2016) Autoimmune regulator (AIRE) is expressed in spermatogenic cells and it altered the expression of several nucleic acid binding and cytoskeletal proteins in GC1-spg spermatogonial cells. Mol Cell Proteomics. doi:10.1074/mcp.M115.052951.
    1. Society of General and Comparative Endocrinology meeting at Delhi University, 1988.
    2. Endocrine Society Conference, Kyoto, Japan, 1988.
    3. Society of Free Radical Biology and Medicine at Bangalore, September 1989.
    4. 51st Annual Meeting of The American Society of Human Genetics, San Diego, CA, USA, October 19-23, 2001.
    5. 23rd Meeting of the American Society of Reproductive Immunology at Yale University, New Haven, CT, USA, 18th-21st June, 2003.
    6. National Seminar on Bioinformatics organized by Distributed Information Sub Center (DISC) at Rajiv Gandhi Centre for Biotechnology, Trivandrum, 14-16 December, 2004.
    7. Co-Chairman, Life Science Session at XVII Kerala Science Congress held at Kerala Forest Research Institute, Peechi, 29-31 January 2005.
    8. National Workshop on Nanoscale Imaging and Detection of Biomolecules at Anna University, Chennai, 17th-19th March, 2005.
    9. Shoeb M, Laloraya M and Kumar PG (2005) Formation of RAFT microdomains in sperm membranes during progesterone-induced acrosome reaction. Poster presented at ISCE 2005 – National Symposium on Comparative Endocrinology and Reproductive Physiology at University of Delhi on November 17-19, 2005.
    10. Health improvement through Plant Biotechnology. Plant Biotechnology-New Vistas in Health Applications. BRSI Second Convention, Anna University, Chennai on 25-27 November 2005.
    11. International Symposium on translational research: Apoptosis and Cancer. 18th to 21st December, 2005.
    12. Shoeb M, Laloraya M and Kumar PG (2006) TRPCs are recruited thorugh RAFT-dependent mechanisms in spdrmatozoa during their post-testicular development. Poster accepted at Biochemical Society Conference on Cell and Molecular Biology of TRP Channels at University of bath, UK on September 7-8, 2006.
    13. Shoeb M, Laloraya M and Kumar PG (2006) NOX-components are associated with sperm membrane RAFTS. Poster accepted at Gordon Research Conference on NOX Family NADPH Oxidases at Les Diablerets, Switzerland on October 15-20, 2006.
    14. Seminar on Biotechnology and Economic Development – A Kerala Scenario. Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala. March 26-26, 2006.
    15. International Symposium on Drug Abuse and HIV/AIDS. Rajiv Gandhi Centre for Biotechnology, Trivandrum. November 6-7, 2006.
    16. Shoeb M, Laloraya M and Kumar PG (2007) Estrogen receptors are associated with sperm membrane rafts. Poster presented in the XXV National Symposium on Reproductive Biology and Comparative Endocrinology, 15-17 January, 2007. Trivandrum.
    17. Chandran U, Laloraya M and Kumar PG (2007) Identification of testis expressed cell cycle regulating proteins with special reference to meiosis. Poster presented in the XXV National Symposium on Reproductive Biology and Comparative Endocrinology, 15-17 January, 2007. Trivandrum. (won 1st prize in poster presentation category)
    18. Chandrasekhar A, Laloraya M and Kumar PG (2007) Role of NADPH oxidase(NOX) in epididymal maturation,capacitation and acrosome reaction of goat spermatozoa. Oral presentation at National symposium on comparitive endocrinology and reproductive physiology at Santiniketan, Nov. 26-28, 2007.
    19. Chandrasekhar A, Laloraya M and Kumar PG (2008) NADPH oxidase organization in goat spermatozoa during epididymal maturation, capacitation and acrosome reaction. Poster presented at 95th Indian science congress, Visakhapatnam, Jan 3-8 2008.
    20. Shah A, Chandrakuntal K, Thomas N, George NM, Varghese DS, Laloraya M, Laloraya MM and Kumar GP (2008) Characterization of NADPH Oxidase and its role in photomorphogenesis in wheat coleoptile tip. Presented at the Interrnational Conference on “Free Radicals and Natural Products in Health (FRNPH-2008) and Seventh Annual Meeting of the Society of Free Radical Research-India” held at the University of Rajasthan (February 14-16, 2008).
    21. Chandran U, Laloraya M and Kumar PG (2008) DNA sequencing and characterization of a novel testis expressed gene clp-1 and its comparison with a brain variant cyclin m1. Presented at 95th Indian Science Congress Association, Jan 3-7, 2008, held at Andhra University, Visakhapatnam.
    22. Indu S, Laloraya M and Kumar PG (2008) Tracing testicular germline stem cells using classical somatic stem cell markers. 95th Indian Science Congress,Andhra University, Visakhapatnam. January 3-7, 2008.
    23. Indu S, Laloraya M and Kumar PG (2008) Scrutiny for stem cell markers in testis: Probable leap towards male infertility treatment. International Symposium on “Model Organisms and Stem Cell in Development, Regeneration and Disease”. NCBS, Bangalore,February 23-25, 2008.
    24. Indu S and Kumar PG (2009) Expression of pluripotency markers in mice testis during post-natal development. International Conference on Germ Cell Development - Function & ISSRA 19th Annual Meeting, Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, January 22-24, 2009.
    25. Bhagya KP and Kumar PG (2011) subcellular distribution and apoptotic activity of deletion constructs of autoimmune regulator ectopically expressed in mouse germ cells. International Conference on Frontiers in Reproductive Biotechnology and 21st Annual Meeting of the Indian Society for the Study of Reproduction and Fertility (ISSRF), Feb 9-11, 2011, National Dairy Research Institute, Karnal, India.
    26. Sreesha Sree and Kumar PG (2011) Profiling of differentially expressed microRNAs in mouse testis in relation to onset of spermatogenesis. International Conference on Frontiers in Reproductive Biotechnology and 21st Annual Meeting of the Indian Society for the Study of Reproduction and Fertility (ISSRF), Feb 9-11, 2011, National Dairy Research Institute, Karnal, India (won best poster award).
    27. Varghese DS and Kumar PG (2011) Analysis of tdp-43 expression in testis in relation to spermatogenesis. International Conference on Frontiers in Reproductive Biotechnology and 21st Annual Meeting of the Indian Society for the Study of Reproduction and Fertility (ISSRF), Feb 9-11, 2011, National Dairy Research Institute, Karnal, India.
    28. Chandran U and Kumar PG (2012) Molecular Cloning and Characterization of a Transcript Variant of cyclin M gene, mtcnnm1, expressed in mouse Testis. 45th SSR Annual Meeting and the 18th Ovarian Workshop, August 12-15, 2012, State College, Pennsylvania (won regional abstract award for papers from India).
    29. Sreesha Sree and Kumar PG (2012) The miRNA-mRNA map of mouse testis in relation to Spermatogenesis. 16th ADNAT convention and Conference on Animal Genetics and Genomics, 16th to 19th December, 2012, National Institute of Animal Biotechnology, Hyderabad (won 3rd prize in the poster session)
    30. Sreesha Sree and Kumar PG (2013) MicroRNAs in mammalian spermatogenesis. International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala.
    31. Devi AN, Kumar ATR, Varghese DS, Thottacherry JJ and Kumar PG (2013) Expression profiling and functional characterization of nephrocystin in relation to spermatogenesis. International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala.
    32. Radhakrishnan K and Kumar PG (2013) Histone modifiers and their role in spermatogenesis. International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala.
    33. Kumar ATR, Oommen OV and Kumar PG (2013) Expression profiling, localization and characterization of protocadherin in fertile and infertile males. International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala.
    34. Indu S and Kumar PG (2013) Establishment of pluripotent nature of adult mouse testicular germline stem cells. International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala.
    35. Varghese DS and Kumar PG (2013) TDP-43 mediated regulation of spermatogenesis. In International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala (won the best poster award)
    36. Bhagya KP and Kumar PG (2013) Functional evaluation of AIRE in germ cell development. In International Conference on Repromics and 23rd Annual Meeting of the Indian Society for the Study of Reproduction and Fertility, 7-9 February 2013, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala.
    37. Yadu N and Kumar PG (2013) An in vitro model to study mechanisms of busulfan induced germ cell death. 21st ECDO Euroconference on Apoptosis on "Cell death:a Biomedical paradigm", Paris, France, September 25-28, 2013.
    38. Sreesha S and Kumar PG (2013) MicroRNA-mRNA interactome regulating mammalian spermatogenesis. 5th EMBO Meeting – Advancing the Life Science, Amsterdam, September 21-24, 2013.
    39. Varghese DS and Kumar PG (2013) Functional evaluation of tdp-43 in spermatogenesis and human male factor infertility. 5th EMBO Meeting – Advancing the Life Science, Amsterdam, September 21-24, 2013.
    40. Sreesha S and Kumar PG (2013) MicroRNA-mRNA interactome regulating mammalian spermatogenesis. International Conference on Developmetn and Genetics, Isle sur la Sorgue, Avignon, France, November 12-15, 2013.
    41. Varaghese DS and Kumar PG (2013) Functional evaluation of tdp-43 in spermatogenesis and human male factor infertility. International Conference on Developmetn and Genetics, Isle sur la Sorgue, Avignon, France, November 12-15, 2013.

    DNA sequences

    1. EF025315. Bombyx mori NADPH oxidoreductase mRNA, partial cds gi|116876142|gb|EF025315.1|[116876142]
    2. NM_004360 Homo sapiens cadherin 1, type 1, E-cadherin (epithelial) (CDH1), mRNA. gi|14589887|ref|NM_004360.2|[1458988]
    3. NM_010927 Mus musculus nitric oxide synthase 2, inducible, macrophage (Nos2), mRNA gi|6754871|ref|NM_010927.1|[6754871]
    4. NM_010876 Mus musculus neutrophil cytosolic factor 1 (Ncf1), mRNA gi|40254579|ref|NM_010876.2|[40254579]
    5. NM_010877 Mus musculus neutrophil cytosolic factor 2 (Ncf2), mRNA gi|31560561|ref|NM_010877.2|[31560561]
    6. NM_011489 Mus musculus signal transducer and activator of transcription 5B (Stat5b), mRNA gi|31543779|ref|NM_011489.2|[31543779]
    7. NM_007807 Mus musculus cytochrome b-245, beta polypeptide (Cybb), mRNA gi|31542439|ref|NM_007807.2|[31542439]
    8. NM_178851 Mus musculus vacuolar protein sorting 39 (yeast) (Vps39), transcript variant 2, mRNA gi|30578383|ref|NM_178851.1|[30578383]
    9. NM_007806 Mus musculus cytochrome b-245, alpha polypeptide (Cyba), mRNA gi|22094076|ref|NM_007806.1|[22094076]
    10. NM_00867 Mus musculus neutrophil cytosolic factor 4 (Ncf4), mRNA gi|6679021|ref|NM_008677.1|[6679021]
    11. NM_000658 Homo sapiens autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) (AIRE), transcript variant AIRE-2, mRNA gi|4557292|ref|NM_000658.1|[4557292]
    12. NM_000383 Homo sapiens autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) (AIRE), transcript variant AIRE-1, mRNA gi|4557290|ref|NM_000383.1|[4557290]
    13. DQ885890 Mus musculus strain Swiss cyclin-like protein 1 mRNA, partial cds gi|115279637|gb|DQ885890.2|[115279637]
    14. NM_013654 Mus musculus chemokine (C-C motif) ligand 7 (Ccl7), mRNA gi|42476054|ref|NM_013654.2|[42476054]
    15. NM_147153 Mus musculus vacuolar protein sorting 39 (yeast) (Vps39), transcript variant 1, mRNA gi|30578430|ref|NM_147153.2|[30578430]
    16. NM_199078 Homo sapiens cyclin M3 (CNNM3), transcript variant 2, mRNA gi|94681053|ref|NM_199078.2|[94681053]
    17. NM_017623 Homo sapiens cyclin M3 (CNNM3), transcript variant 1, mRNA gi|94681052|ref|NM_017623.4|[94681052]
    18. NM_020184 Homo sapiens cyclin M4 (CNNM4), mRNA gi|94681045|ref|NM_020184.3|[94681045]
    19. NM_020348 Homo sapiens cyclin M1 (CNNM1), mRNA gi|9966788|ref|NM_020348.1|[9966788]
    20. NM_199076 Homo sapiens cyclin M2 (CNNM2), transcript variant 2, mRNA gi|40068054|ref|NM_199076.1|[40068054]
    21. NM_017649 Homo sapiens cyclin M2 (CNNM2), transcript variant 1, mRNA gi|40068052|ref|NM_017649.3|[40068052]
    22. NM_199077 Homo sapiens cyclin M2 (CNNM2), transcript variant 3, mRNA gi|40068050|ref|NM_199077.1|[40068050]
    23. AY042906 Mus musculus signal transducer and activator of transcription 5b (Stat5b) mRNA, complete cds gi|21654874|gb|AY042906.1|[21654874]
    24. AY040231 Mus musculus signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds gi|21654824|gb|AY040231.1|[21654824]
    25. AY044903 Mus musculus strain C3H signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds gi|21654906|gb|AY044903.1|[21654906]
    26. AY044902 Mus musculus strain CBA signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds gi|21654904|gb|AY044902.1|[21654904]
    27. AY044901 Mus musculus strain BALB/c signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds gi|21654902|gb|AY044901.1|[21654902]
    28. AF058292 Homo sapiens tRNA-Phe gene, mitochondrial gene for mitochondrial RNA, partial sequence gi|3047403|gb|AF058292.1|AF058292[3047403]
    29. U53183 Rattus norvegicus 250 kDa estrous-specific protein mRNA, partial cds gi|1279987|gb|U53183.1|RNU53183[1279987]
    30. U59758 Mus musculus p53-variant (p53) mRNA, partial cds gi|1399879|gb|U59758.1|MMU59758[1399879]
    31. U59757 Mus musculus p53 cellular tumor antigen (p53) mRNA, partial cds gi|1399877|gb|U59757.1|MMU59757[1399877]
    32. U52869 Mus musculus implantin mRNA, partial cds gi|1256825|gb|U52869.1|MMU52869[1256825]

    Protein Sequences

    1. ABI34706 cyclin-like protein 1 [Mus musculus] gi|115279638|gb|ABI34706.2|[115279638]
    2. AAB03324 p53 transformation associated protein gi|1399880|gb|AAB03324.1|[1399880]
    3. AAB03323 p53 cellular tumor antigen gi|1399878|gb|AAB03323.1|[1399878]
    4. ABK30932 NADPH oxidoreductase [Bombyx mori] gi|116876143|gb|ABK30932.1|[116876143]
    5. NP_035619 signal transducer and activator of transcription 5B [Mus musculus] gi|31543780|ref|NP_035619.2|[31543780]
    6. AAL05590 signal transducer and activator of transcription 5b [Mus musculus] gi|21654875|gb|AAL05590.1|[21654875]
    7. AAK74074 signal transducer and activator of transcription 5B [Mus musculus] gi|21654825|gb|AAK74074.1|[21654825]
    8. AAK97793 signal transducer and activator of transcription 5B [Mus musculus] gi|21654907|gb|AAK97793.1|[21654907]
    9. AAK97792 signal transducer and activator of transcription 5B [Mus musculus] gi|21654905|gb|AAK97792.1|[21654905]
    10. AAK97791 signal transducer and activator of transcription 5B [Mus musculus] gi|21654903|gb|AAK97791.1|[21654903]
    11. AAC13319 estrous-specific protein [Rattus norvegicus] gi|1279988|gb|AAC13319.1|[1279988]
    12. AAA96527 implantin [Mus musculus] gi|1256826|gb|AAA96527.1|[1256826]
    13. NP_035057 nitric oxide synthase 2, inducible, macrophage [Mus musculus] gi|6754872|ref|NP_035057.1|[6754872]
    14. NP_004351 cadherin 1, type 1 preproprotein [Homo sapiens] gi|4757960|ref|NP_004351.1|[4757960]
    15. NP_035006 neutrophil cytosolic factor 1 [Mus musculus] gi|40254580|ref|NP_035006.2|[40254580]
    16. NP_031833 cytochrome b-245, beta polypeptide [Mus musculus] gi|31542440|ref|NP_031833.2|[31542440]
    17. NP_849182 vacuolar protein sorting 39 isoform 2 [Mus musculus] gi|30578384|ref|NP_849182.1|[30578384]
    18. NP_031832 cytochrome b-245, alpha polypeptide [Mus musculus] gi|22094077|ref|NP_031832.1|[22094077]
    19. NP_035007 neutrophil cytosolic factor 2 [Mus musculus] gi|6754798|ref|NP_035007.1|[6754798]
    20. NP_032703 neutrophil cytosolic factor 4 [Mus musculus] gi|6679022|ref|NP_032703.1|[6679022]
    21. Q9Z0E3 Autoimmune regulator (Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog) (APECED protein homolog) gi|22256596|sp|Q9Z0E3|AIRE_MOUSE[22256596]
    22. NP_000649 autoimmune regulator AIRE isoform 2 [Homo sapiens] gi|4557293|ref|NP_000649.1|[4557293]
    23. NP_000374 autoimmune regulator AIRE isoform 1 [Homo sapiens] gi|4557291|ref|NP_000374.1|[4557291]
    24. O43918 Autoimmune regulator (Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein) (APECED protein) gi|3334119|sp|O43918|AIRE_HUMAN[3334119]
    25. P42232 Signal transducer and activator of transcription 5B gi|1174465|sp|P42232|STA5B_MOUSE[1174465]
    26. NP_038682 chemokine (C-C motif) ligand 7 [Mus musculus] gi|7305463|ref|NP_038682.1|[7305463]
    27. NP_671495 vacuolar protein sorting 39 isoform 1 [Mus musculus] gi|22164794|ref|NP_671495.1|[22164794]
    28. P83891 Implantin gi|50400835|sp|P83891|IMPL_MOUSE[50400835]
    29. P83853 Vam6-like protein gi|46396754|sp|P83853|VAM6L_MOUSE[46396754]
    30. P83854 Putative G-protein coupled receptor gi|46395688|sp|P83854|GPCR_MOUSE[46395688]
    31. P84517 Putative NADH-ubiquinone oxidoreductase chain 4-like protein gi|62900716|sp|P84517|NU4ML_BOMMO[62900716]
    32. A59495 Vesicle associated membrane protein – Mus musculus gi|60729662|pir||A59495[60729662]
    33. P83855 Putative sperm adenylate cyclase gi|46395689|sp|P83855|ACLP_MOUSE[46395689]
    1. National Congress organized by Indian Society of Plant Physiology at Devi Ahilya University, Indore in 1994
    2. International Congress on Fertilization, Embryo Development and Implantation, held at National Institute of Immunology, New Delhi, 6-9 November, 2000.
    3. International Conference on Steroid Hormone Receptor Mediated Signaling (SHRMS) held at Rajiv Gandhi Centre for Biotechnology, Trivandrum, 25-27 November, 2004.
    4. National Seminar on Bioinformatics organized by Distributed Information Sub Center (DISC) at Rajiv Gandhi Centre for Biotechnology, Trivandrum, 14-16 December, 2004.
    5. National Workshop on Nanoscale Imaging and Detection of Biomolecules at Anna University, Chennai, 17th-19th March, 2005.
    6. Health improvement through Plant Biotechnology. Plant Biotechnology-New Vistas in Health Applications. BRSI Second Convention, Anna University, Chennai on 25-27 November 2005.
    7. Scientific aspects of gene cloning. Regional Seminar on The Social, Scientific and Ethical Implications of Gene Cloning. NSS College for Women, Neeramankara, Trivandrum and University Grants Commission, New Delhi. 12-14 July, 2006.
    8. Gene cloning. Science day at St. Thomas School, Mukkolakkal, Trivandrum, 2006.
    9. Expression and silencing of genes. National Seminar on Gene Technology for Society. Department of Botany and Biotechnology, Mar Ivanios College, Trivandrum. 2 November, 2006.
    10. Translational Repromics in contraceptive development. XXV National Symposium on Reproductive Biology and Comparative Endocrinology and University of Kerala, Trivandrum. 15-17 January, 2007
    11. Cloning: Dolly and beyond. Science day at St. Thomas School, Mukkolakkal, Trivandrum, 2006.
    12. Proteomics in Biology and Medicine. Science day at St. Berckman’s College, Chanagacherry, 2007
    13. Germline stem cells and their prospects. Society of Biotechnologists India Conference, 2007
    14. Molecular defects in sperm and human male factor infertility. DBT-National workshop on human infertility and reproductive disorders at Indian Instiute of Science, Bangalore, November 29-30, 2007.
    15. Proteomics in Medicine. National Seminar on Medical Biotechnology, KVVS College of science and technology, Adoor, 12 Dec 2007
    16. Techiques and Tools in Proteomics. MedicoVentururs, Sri Buddha College, Padanilam, 2007.
    17. The Science and Ethics of Stem Cell Research. Science Day 2008 lecture at Mar Ivanios College, Trivandrum. 13 Jan 2008.
    18. What is so special about us humans? Science Day 2008 lecture at Rajiv Gandhi Centre for Biotechnology, Trivandrum, 28 February 2008.
    19. Information-aided research in Repromics at National Technical Conferene on Avenues for Research and Development in Biotechnology, REC College, Chennai, 22 August 2008.
    20. Gene Silencing. FCBS lecture at St. Thomas Central School, Trivandrum, 20 Sept, 2008.
    21. Information-aided research in Repromics at State Level Seminar and Workshop on Biological Techniques, Mar Athanasius College, Kothamangalam, 2 Nov 2008.
    22. Biotechnology: Good or Bad? 18th Swadeshi Sacience Congress, Rajiv Gandhi Centre for Biotechnology, 5-7 Nov 2008.
    23. Biotechnology: Good or Bad? One Day Seminar on Genomics and Transgenics – Challenges and Prospects, Department of Biotechnology, University of Kerala, Trivandrum, 14 Nov 2008.
    24. Biotechnology: Good or Bad? CSIR Program on Youth for Leadership in Science-2008, NIIST, Trivandrum, Dec 22-23, 2008
    25. Germline stem cells: fact or fiction? International Conference On Novel Updates In Reproductive Biology And Comaparative Endocrinology And The 27th Annual Meeting Of The Society For Reproductive Biology And Comparative Endocrinology (SRBCE-XXVII) January 19-21, 2009, University Of Hyderabad, India.
    26. Search for factors regulating meiosis in mammalian testis. International Conference on Germ Cell Development – Function & ISSRA 19th Annual Meeting, Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, January 22-24, 2009
    27. Germline Stem Cells – Fact or Fiction? A Day with Stalworts. Joint Indo-US Centre Programme on Stem Cells and Tissue Engineering, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Trivandrum, Jan 27, 2009.
    28. Mohammed Shoeb, Malini Laloraya, and Pradeep G Kumar (2009) Progesterone-induced reorganization of NOX-2 components in sperm membrane rafts is critical for sperm functioning. National Symposium on Comparative Endocrinology and Reproductive Physiology: Current Advances, Department of Zoology, The Institute of Life Science, Mumbai, 17-19 December, 2009.
    29. Kumar PG (2010) Germline Stem cells. National Seminar on Neurobiology and Stem Cells. Indo-American College, Cheyyar, March 5-6, 2010.
    30. Chandrasekhar A, Laloraya M and Kumar PG (2010) Membrane modifications in goat spermatozoa during capacitation and acrosome reaction are linked with the modulation of NOX2 activity through post-translational processing of regulatory subunits. SAPI Silver Jubilee and International Conference on Physiological capacity building in livestock under changing climatic scenario”, November 11-13, 2010, Indian Veterinary Research Institute, Izatnagar, India.
    31.  Kumar PG (2011) Organization and functional significance of lipid rafts in sperm membranes. International Conference on Frontiers in Reproductive Biotechnology and 21st Annual Meeting of the Indian Society for the Study of Reproduction and Fertility (ISSRF), Feb 9-11, 2011, National Dairy Research Institute, Karnal, India.
    32. Shoeb M and Kumar PG (2011) Progesterone-induced reorganization of NOX-2 components in membrane rafts is critical for sperm functioning in Capra hircus. 12th Congress on Reproductive Biomedicine, 7-9 September 2011, Tehran, Iran.
    33.  Kumar PG (2011) Molecular markers of germline stem cells from mouse testis. XXXV All India Cell Biology Conference (AICB) and Symposium on Membrane Dynamics and Disease, 16-18 Dec 2011. National Institute of Science Education and Research (NISER), Bhubaneswar, Orissa, India.
    34. Kumar PG (2011) Where to go? Inspire Winter Science Camp for Plus One School Children, A DST, Govt. of India Program, Dec 20-24, 2011. National Institute of Technology, Calicut, Kerala.
    35.  Kumar PG (2012) Life style and infertility. National Seminar on Life style and Diseases (In connection with INBOFA-2012), School of Biosciences, Mahatma Gandhi University, Kottayam, Jan 19-20, 2012.
    36. Kumar PG (2012) Germline stem cells - Prospects in therapeutics. BV Patel PERD Centre, Ahmedabad, May 26, 2012.
    37. Kumar PG (2012) Germlien stem cells - Prospects in therapeutics. 12th International Conference on What is new in managing subfertility, obstetrics and gynaecology, 3-5 August, 2012. Madras Medical Mission, Chennai.
    38. Kumar PG (2012) Molecular Markers of Gerline stem cells from mouse testis. National Workshop on Stem Cell Research and Therapeutics: Current Status and Future Strategies. Indian Veterinary Research Institute, Izatnagar, Bareilly, Sept 28-29, 2012.
    39.  Kumar PG (2013) Towards identifying molecular markers for human male factor subfertility. ANKUR-SASSM Oration at the Inaugural conference of the South Asian Society for Sexual Medicine (SASSM), Indian Institute of Science, Bangalore, March 29-31, 2013.
    40. Kumar PG (2013) Where to go? Inspire Internship Science Camp, St. Xaviers Catholic College of Engineering, Chunkankadai, Nagercoil, Tamilnadu, 15-19 July, 2013.
    41. Kumar PG (2013) MicroRNA-mRNA networks during the initiation of 1st wave of spermatogenesis in mouse testis. National Symposium on Recent Advances in Reproductive Health, Centre for Advanced Studies in Zoology, Banaras Hindu University, November 28-30, 2013.
    42. Kumar PG (2013) MicroRNA-mRNA networks during 1st wave of spermatogenesis in mouse testis. 82nd Annual Meeting of the Society of Biological Chemists (India) and International Symposium on Genomes: Mechanism and Function, School of Life Sciences, University of Hyderabad, India, December 2-5, 2013.
    43. Kumar PG (2014) MicroRNA-mRNA networks during 1st wave of spermatogenesis in mouse testis. International Conference on Reproductive Health: Issues and Strategies under Changing Climate Scenario, and the 24th Annual Meeting of Indian Society for Study of Reproduction and Fertility (ISSRF), 6-8 February, 2014 at Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, India.
    44. Kumar PG (2014) Where to go? Inspire Internship Science Camp, St. Xaviers Catholic College of Engineering, Chunkankadai, Nagercoil, Tamilnadu, 14-18 July, 2014.
    45. Kumar PG (2015) Where to go? Inspire Internship Science Camp, SN College, Kannur, Kerala, 23-28 December, 2014
    46. Bhagya KP, Radhakrishnan K, Yadu N and Kumar PG (2015) There is AIRE in the testis. What is its business there? Labhsetwar Award lecture at the 25th Annual Meeting of Indian Society for the Study of Reproduction and Fertility and International Conference on Reproductive Health, National Institute for Research in Reproductive Health, Parel, Mumbai, 14-17 February, 2015.
    47. Kumar PG (2015) Stem cells: Past, Present and Future. At Central University of Rajasthan, Ajmer, Rajasthan, 11 September 2015.
    48. Kumar PG (2015) Molecular markers for male factor infertility. National Conference on Reproductive Health Challenges: Issues and Remedies (NCRHC-2015), IIS University, Jaipur, 11-13 September, 2015.
    49. Kumar PG (2015) Panel discussion on Biotechnology. Swasraya Bharat 2015, Swapna Nagari, Kozhikode, 15-21 October, 2015.
    50. Kumar PG (2016) Differential display proteomics of spermatozoa: our leads in understanding male factor infertility. Dr. TC Anand Kumar Memorial Gold Medal Oration at the 26th Annual Meeting of Indian Society for the Study of Reproduction and Fertility, National Institute of Occupational Health, Ahmedabad, 18-20 February, 2016.
    51. Kumar PG (2016) Identification of Molecular Markers of Male Factor Infertility. National Institute for Science, Education and Research, Bhuhaneswar, 17-18 May, 2016.
    52. Kumar PG (2016) Where to go? Mar Ivanios College, Trivandrum, 11 June, 2016.
  • Team


    Karthika Radhakrishnan, PhD Student

    Sperm development and differentiation in mammalian testis is a unique event. Seminiferous tubules in the testis have germ cell progenitors known as primordial germ cells (PGCs), which are pluripotent stem cells. These cells can divide horizontally to make their own copies, but also can differentiate into spermatogonia. Spermatogonia retain a high level of multipotency, and can divide horizontally to make their copies. Through unknown mechanisms operating at their cellular level, they can differentiate into spermatozoa. While hormonal and local regulations operating on them are implicated in their differentiation, very little is known about the factors that regulate the transformation of a spermatogonium into primary spermatocyte. Similarly, factors that regulate the differentiation of primary spermatocyte into secondary spermatocyte also are intriguing. I focus on molecular aspects of initiation of meiosis in spermatogenic cells.

    karthika radhakrishnan
    karthika radhakrishnan

    Karthika Radhakrishnan, PhD Student

    Sperm development and differentiation in mammalian testis is a unique event. Seminiferous tubules in the testis have germ cell progenitors known as primordial germ cells (PGCs), which are pluripotent stem cells. These cells can divide horizontally to make their own copies, but also can differentiate into spermatogonia. Spermatogonia retain a high level of multipotency, and can divide horizontally to make their copies. Through unknown mechanisms operating at their cellular level, they can differentiate into spermatozoa. While hormonal and local regulations operating on them are implicated in their differentiation, very little is known about the factors that regulate the transformation of a spermatogonium into primary spermatocyte. Similarly, factors that regulate the differentiation of primary spermatocyte into secondary spermatocyte also are intriguing. I focus on molecular aspects of initiation of meiosis in spermatogenic cells.

    Anil Kumar T R, PhD Student

    Our laboratory has identified a cadherin on spermatozoa from healthy and fertile human males, which was absent or underexpressed in oligozoospermic human males. Though N-terminal sequence analysis yielded homology with E-cadherin due to the presence of conserved EC repeats in all types of cadherins, subsequent RT-PCR analysis of the message in the testis revealed that this differentially displayed protein is a member of protocadherins. My objectives are to conduct semiquantitative RT-PCR analysis of human testis-expressed protocadherin (htpCadherin) in germ line collected from semen, sequence the full length message of htpCadherin and detect any defect(s) within the coding region of this message in infertile (oligozoospermic and idiopathic) males. I would also clone the full length htpCadherin gene into pTYB1 vector, express and purify the recombinant protein for functional studies.

    anil
    anil

    Anil Kumar T R, PhD Student

    Our laboratory has identified a cadherin on spermatozoa from healthy and fertile human males, which was absent or underexpressed in oligozoospermic human males. Though N-terminal sequence analysis yielded homology with E-cadherin due to the presence of conserved EC repeats in all types of cadherins, subsequent RT-PCR analysis of the message in the testis revealed that this differentially displayed protein is a member of protocadherins. My objectives are to conduct semiquantitative RT-PCR analysis of human testis-expressed protocadherin (htpCadherin) in germ line collected from semen, sequence the full length message of htpCadherin and detect any defect(s) within the coding region of this message in infertile (oligozoospermic and idiopathic) males. I would also clone the full length htpCadherin gene into pTYB1 vector, express and purify the recombinant protein for functional studies.

    Nomesh Yadu, PhD Student

    Retinoic acid has been demonstrated to be the main factor causing the differentiation of SSCs in the mammalian testis. Retinoic acid exerts its effect through many of the receptors present in Sertoli cells and germ cells. RA receptors are broadly catagorised as Retinoic Acid Receptors (RARs) and Rexinoid receptors (RXRs) and both types have been shown to be expressed in the mammalian testis. RARs are of three types, viz, RAR? RAR? and RAR?. RA-dependent events are believed to be mediated predominantly by RA receptor ? (RAR ?) in spermatogonia and by RAR? in Sertoli cells. Recent reports indicate that RAR? may function in Sertoli cells to promote the survival and development of early meiotic prophase spermatocytes, whereas RAR? in germ cells functions to increase the proliferation and differentiation of spermatogonia, prior to meiotic prophase. However, RAR?-dependent pathways operating in Sertoli cells which ultimately affect the germline lineage at various stages of their development are not clear. In this context, we aim to study the specific roles of retinoic acid receptors ? and ? in germ cell differentiation using in vitro culture systems and selective silencing of respective receptor genes.

    nomesh
    nomesh

    Nomesh Yadu, PhD Student

    Retinoic acid has been demonstrated to be the main factor causing the differentiation of SSCs in the mammalian testis. Retinoic acid exerts its effect through many of the receptors present in Sertoli cells and germ cells. RA receptors are broadly catagorised as Retinoic Acid Receptors (RARs) and Rexinoid receptors (RXRs) and both types have been shown to be expressed in the mammalian testis. RARs are of three types, viz, RAR? RAR? and RAR?. RA-dependent events are believed to be mediated predominantly by RA receptor ? (RAR ?) in spermatogonia and by RAR? in Sertoli cells. Recent reports indicate that RAR? may function in Sertoli cells to promote the survival and development of early meiotic prophase spermatocytes, whereas RAR? in germ cells functions to increase the proliferation and differentiation of spermatogonia, prior to meiotic prophase. However, RAR?-dependent pathways operating in Sertoli cells which ultimately affect the germline lineage at various stages of their development are not clear. In this context, we aim to study the specific roles of retinoic acid receptors ? and ? in germ cell differentiation using in vitro culture systems and selective silencing of respective receptor genes.

    Soumya A, PhD Student

    The primary interaction between mammalian spermatozoa and oocyte is mediated by partially defined sperm components and four major components of zona pellucida (ZP1, ZP2, ZP3 and ZP4). My aim is to characterize the molecular interactions between ZP proteins and spermatozoa. Using ZP proteins and sperm membrane components tagged with appropriate fluorescent reporters, we envisage to study the physical responses of sperm membranes to ZP binding and the downstream signalling events which follow this binding.

    sumya
    sumya

    Soumya A, PhD Student

    The primary interaction between mammalian spermatozoa and oocyte is mediated by partially defined sperm components and four major components of zona pellucida (ZP1, ZP2, ZP3 and ZP4). My aim is to characterize the molecular interactions between ZP proteins and spermatozoa. Using ZP proteins and sperm membrane components tagged with appropriate fluorescent reporters, we envisage to study the physical responses of sperm membranes to ZP binding and the downstream signalling events which follow this binding.

    Devi A N, PhD Student

    NPHP1 is a differentially displayed protein expressed in human testis and its absence is associated with spermatogenic insufficiency in men. This protein is coded by nphp1, and is known to be a cilial protein and acomponent of adherans junctions. However, the presence of multiple functional domains in this protein and it interacts with crk associated substrate (p130cas alias BCAR1), nephrocystin-4, Pyk2, tensin, filamin A, filamin B and PACS-1 makes it an important candidate molecule for further investigations. Nphp1 is expressed specifically in post-meiotic germ cells murine testis, while expression is absent in sertoli cells, interstitial leydig cells and pre-meiotic germ cells. The presence of Mad_BUB1_I (Mad3/BUB1 hoMad3/BUB1 homology region 1) and helix loop helix domain (HLH) in NPHP1 prompts us to speculate it involvement as a possible check point protein in cell division. We propose to investigate the role of NPHP1 in germ cell division, differentiation and sperm functioning.

    devi1
    devi1

    Devi A N, PhD Student

    NPHP1 is a differentially displayed protein expressed in human testis and its absence is associated with spermatogenic insufficiency in men. This protein is coded by nphp1, and is known to be a cilial protein and acomponent of adherans junctions. However, the presence of multiple functional domains in this protein and it interacts with crk associated substrate (p130cas alias BCAR1), nephrocystin-4, Pyk2, tensin, filamin A, filamin B and PACS-1 makes it an important candidate molecule for further investigations. Nphp1 is expressed specifically in post-meiotic germ cells murine testis, while expression is absent in sertoli cells, interstitial leydig cells and pre-meiotic germ cells. The presence of Mad_BUB1_I (Mad3/BUB1 hoMad3/BUB1 homology region 1) and helix loop helix domain (HLH) in NPHP1 prompts us to speculate it involvement as a possible check point protein in cell division. We propose to investigate the role of NPHP1 in germ cell division, differentiation and sperm functioning.

    Mahita Sahadevan, PhD Student (UGC-SRF)

    Our laboratory has recently reported dramatic changes in microRNA and mRNA profiles in mouse testis during the initiation of the first waves of spermatogenesis. The differentially expressed sets of 67 miRNAs and 8,226 mRNAs were integrated into miRNA-mRNA networks, which appeared to have functional significance in controlling regulatory pathways associated with germ cell division and differentiation. My study will focus on mRNA clusters around prominent miRNA nodes which include miR-34b-5p, miR-34c, miR-200a and miR-449a. The targets of several of the differentially displayed miRNAs were molecules involved in cellular signalling and transcription. We plan to offset the levels of the differentially displayed miRNAs using miRNA mimics and/or sponges to see its effect on target gene expression profiles and the fate of germ cells.

    mahita
    mahita

    Mahita Sahadevan, PhD Student (UGC-SRF)

    Our laboratory has recently reported dramatic changes in microRNA and mRNA profiles in mouse testis during the initiation of the first waves of spermatogenesis. The differentially expressed sets of 67 miRNAs and 8,226 mRNAs were integrated into miRNA-mRNA networks, which appeared to have functional significance in controlling regulatory pathways associated with germ cell division and differentiation. My study will focus on mRNA clusters around prominent miRNA nodes which include miR-34b-5p, miR-34c, miR-200a and miR-449a. The targets of several of the differentially displayed miRNAs were molecules involved in cellular signalling and transcription. We plan to offset the levels of the differentially displayed miRNAs using miRNA mimics and/or sponges to see its effect on target gene expression profiles and the fate of germ cells.

    Irfan Khan P, PhD Student (DST-INSIPRE JRF)

    Cyclin M1 (CMMN1) is a cyclin-like protein expressed in testis and brain. Being a putative cell cycle regulator this protein is expected to shunt between cytoplasm and nucleus in a cell cycle specific manner. CNNM1 is predicted to have a nuclear localization signal (amino acids 381-385). It is possible that CNNM1 will interact with specific partner kinases (cdk family molecules) to produce active kinase complexes with would alter the phosphoproteome landscape of the cell. In this study, we would aim to examine the subcellular distribution of CNNM1, functional competence of its putative nuclear localization signal, cell cycle progression resulting from CNNM1 over-expression/silencing, interactions of CNNM1 with other cellular proteins and the resulting phosphoproteome level alterations in a mouse spermatogenic cell line (GC1).

    irfan
    irfan

    Irfan Khan P, PhD Student (DST-INSIPRE JRF)

    Cyclin M1 (CMMN1) is a cyclin-like protein expressed in testis and brain. Being a putative cell cycle regulator this protein is expected to shunt between cytoplasm and nucleus in a cell cycle specific manner. CNNM1 is predicted to have a nuclear localization signal (amino acids 381-385). It is possible that CNNM1 will interact with specific partner kinases (cdk family molecules) to produce active kinase complexes with would alter the phosphoproteome landscape of the cell. In this study, we would aim to examine the subcellular distribution of CNNM1, functional competence of its putative nuclear localization signal, cell cycle progression resulting from CNNM1 over-expression/silencing, interactions of CNNM1 with other cellular proteins and the resulting phosphoproteome level alterations in a mouse spermatogenic cell line (GC1).

    Jeeva S E, PhD Student (DBT-JRF)

    It is believed that adult somatic stem cells (SoSC) possess error-prone DNA repair mechanisms which would offer them longevity at the cost of compromised genome integrity and reduced functional competence as time progresses. However, it is more challenging for the spermatogonial stem cells (SpSCs) to maintain the integrity of their genome to prevent reproduction failure and limit the hereditary risk associated with transmission to the progeny. SSCs must therefore have robust response mechanisms to counteract the potentially deleterious effects of DNA damage, with DNA double-strand breaks (DSBs) representing the greatest threat to genomic integrity. Since DSB is the root cause of aging in the living system, a highly efficient DSB repair mechanism should arguably retard -if not stop- aging in the SpSCs. I focus on the aging processes in SoSc versus SpSC in a mouse model.

    jeeva
    jeeva

    Jeeva S E, PhD Student (DBT-JRF)

    TCTEX1 is a gene associated with male sterility in mouse and human, and is expressed heavily in the testis and moderately in the brain. Though it was originally thought to be a part of dyenin motor complex, recent studies have indicated its association with stemness in neural cell progenitors. Expression of TCTEX1 in brain is limited to Neural cell progenitors, and suppression of its expression leads neuronal stem cells into the differentiation pathway. A 100-fold higher level of expression of TCTEX1 in adult testis when compared with other tissues, and the remarkable link between aberrant expression of TCTEX1 and impaired spermatogenesis in mouse and human prompt us to hypothesize that TCTEX1 might dictate stemness in germ cell progenitors, and that the absence of sufficient quantities of quality germ cell progenitors could be the root cause of spermatogenic defects in TCTEX1-defective animals and patients. This study is outlined to address whether TCTEX1 has any role to play in deciding on the stemness of germ cell progenitors in mouse testis.

    Aswathy J R, PhD Student (CSIR-JRF)

    My project aims to decipher the possible transcriptional and post-transcriptional regulation of gene expression by Autoimmune Regulator (AIRE) in spermatogonial (Spg) cells. AIRE will be expressed ectopically in GC1-spg cells and its binding to DNA will be studied using ChIPSeq. The influence of AIRE on gene expression in GC1-spg cells will be addressed using whole transcriptome profiling followed by a network building of using this dataset and the corresponding proteome dataset already available with us. We will also manipulate AIRE expression in primary cultures of Spg cells and the resulting changes in the gene expression will be assessed using ChIPSeq and mRNP-IP and proteome profiling. The DNA/RNA sequences in the pull-down fraction will be characterized using ChIP-on-CHIP, ChIP-Seq and RNA-Seq. The proteins in the complex will be characterized using LC-MS/MS analysis.

    aswathy
    aswathy

    Aswathy J R, PhD Student (CSIR-JRF)

    My project aims to decipher the possible transcriptional and post-transcriptional regulation of gene expression by Autoimmune Regulator (AIRE) in spermatogonial (Spg) cells. AIRE will be expressed ectopically in GC1-spg cells and its binding to DNA will be studied using ChIPSeq. The influence of AIRE on gene expression in GC1-spg cells will be addressed using whole transcriptome profiling followed by a network building of using this dataset and the corresponding proteome dataset already available with us. We will also manipulate AIRE expression in primary cultures of Spg cells and the resulting changes in the gene expression will be assessed using ChIPSeq and mRNP-IP and proteome profiling. The DNA/RNA sequences in the pull-down fraction will be characterized using ChIP-on-CHIP, ChIP-Seq and RNA-Seq. The proteins in the complex will be characterized using LC-MS/MS analysis.

    Vysakh G (KSCSTE-JRF)

    Obesity is a major public health problem in India and more so in Kerala and contributes as a major risk factor for non communicable diseases such as diabetes, hypertension, cardiovascular diseases, cancer and metabolic syndrome. It is well known that obesity decreases fertility in several ways in both men and women. Fertility rates started declining in India, especially in Kerala in the last two decades. Gene environment interaction has been hypothesized as a risk factor in the causation web for obesity. There are absolutely no studies relating polymorphisms in obesity-associated genes and infertility. My studies will focus on SNPs of selected candidate genes and their association with obesity and/or spermatogenic impairment.

    TessyAnn

    Vysakh G (KSCSTE-JRF)

    Obesity is a major public health problem in India and more so in Kerala and contributes as a major risk factor for non communicable diseases such as diabetes, hypertension, cardiovascular diseases, cancer and metabolic syndrome. It is well known that obesity decreases fertility in several ways in both men and women. Fertility rates started declining in India, especially in Kerala in the last two decades. Gene environment interaction has been hypothesized as a risk factor in the causation web for obesity. There are absolutely no studies relating polymorphisms in obesity-associated genes and infertility. My studies will focus on SNPs of selected candidate genes and their association with obesity and/or spermatogenic impairment.

  • Alumni


    Post-Doctoral Fellows

    Dr. Manish Nivsarkar (Post-Doctoral Associate in 1994)

    Joined as a Post-Doctoral Associate and worked on the effect of secondary plant metabolites on sperm motility. Scientist at MRS Hurkosondas Narrotamdas Hospital, Bombay (1993-1996). Scientist at PERD Centre, Ahmedabad (2000-2012). Director, PERD Centre, Ahmedabad (2013-present). Link to http://www.perdcentre.com/director.html

    Manish Nivsarkar
    Manish Nivsarkar

    Dr. Manish Nivsarkar (Post-Doctoral Associate in 1994)

    Joined as a Post-Doctoral Associate and worked on the effect of secondary plant metabolites on sperm motility. Scientist at MRS Hurkosondas Narrotamdas Hospital, Bombay (1993-1996). Scientist at PERD Centre, Ahmedabad (2000-2012). Director, PERD Centre, Ahmedabad (2013-present). Link to http://www.perdcentre.com/director.html

    Dr. Sunita Agnihotri (Post-doctoral associate during 1998-2000 and 2002-2006)

    Identified and characterized NAD(P)H-dependent speroxide production in spermatozoa. Mapped the distribution and reorganizations in the distribution of this protein on the spermatozoa in relation to epididymal maturation, capacitation and acrosome reaction. Cloned and partially sequenced testis-expressed NOX-5. Currently working as a Visiting Scientist at Centre for Advanced Technology, Indore, Madhya Pradesh.

    Sunita Agnihotri
    Sunita Agnihotri

    Dr. Sunita Agnihotri (Post-doctoral associate during 1998-2000 and 2002-2006)

    Identified and characterized NAD(P)H-dependent speroxide production in spermatozoa. Mapped the distribution and reorganizations in the distribution of this protein on the spermatozoa in relation to epididymal maturation, capacitation and acrosome reaction. Cloned and partially sequenced testis-expressed NOX-5. Currently working as a Visiting Scientist at Centre for Advanced Technology, Indore, Madhya Pradesh.

    Dr. Anugya Mehta (2006) Post-Doctoral Fellow

    Completed PhD in Biotechnology in 2006 from Jiwaji University, Gwalior on Studies on chlorpyrifos induced oxidative stress and oxidative DNA damage in rat brain and search of a suitable biomarker, under Dr. Nalini Srivastava. Post-doctoral assignment involved the characterization of Erk1/2 activation and downstream signaling pathways during sperm development. Post-doctoral fellow at Sree Chitra Tirunal Institute for Medical Science and Technology (SCTIMST), Thiruvananthapuram (2007). Working as a Scientist at Sree Chitra Tirunal Institute for Medical Science and Technology (SCTIMST), Thiruvananthapuram (2008-present). Link to: SCTIMST

    Anugya Mehta
    Anugya Mehta

    Dr. Anugya Mehta (2006) Post-Doctoral Fellow

    Completed PhD in Biotechnology in 2006 from Jiwaji University, Gwalior on Studies on chlorpyrifos induced oxidative stress and oxidative DNA damage in rat brain and search of a suitable biomarker, under Dr. Nalini Srivastava. Post-doctoral assignment involved the characterization of Erk1/2 activation and downstream signaling pathways during sperm development. Post-doctoral fellow at Sree Chitra Tirunal Institute for Medical Science and Technology (SCTIMST), Thiruvananthapuram (2007). Working as a Scientist at Sree Chitra Tirunal Institute for Medical Science and Technology (SCTIMST), Thiruvananthapuram (2008-present). Link to: SCTIMST

    Dr. C. Jasmin (2008-2009) Post-Doctoral Fellow

    Involved in the cloning and expression of full-length clp-2 and clp-3 from mouse and human testicular germ cells. Also involved in the cloning and expression of cyclin b2 and a few polyubiquitins. Currently working as a DST Woman Scientist at National Institute of Oceanography Regional Centre, Kochi.

    C. Jasmin
    C. Jasmin

    Dr. C. Jasmin (2008-2009) Post-Doctoral Fellow

    Involved in the cloning and expression of full-length clp-2 and clp-3 from mouse and human testicular germ cells. Also involved in the cloning and expression of cyclin b2 and a few polyubiquitins. Currently working as a DST Woman Scientist at National Institute of Oceanography Regional Centre, Kochi.

    Dr. Indu S (2013-2015)

    Evaluated the genome stability in testicular germ line stem cells in a mouse model. Currently, she is a Post-Doctoral Fellow at University of Toledo, Ohio.

    indu
    indu

    Dr. Indu S (2013-2015)

    Evaluated the genome stability in testicular germ line stem cells in a mouse model. Currently, she is a Post-Doctoral Fellow at University of Toledo, Ohio.

    PhDs

    Dr. Aparna Singh (1989-1993)

    Obtained PhD in 1993. Worked on structural changes in sperm membranes during epididymal maturation, capacitation and acrosome reaction. After completing PhD, joined as a Scientist with Indian Army. Currently, working as Deputy Registrar at Centre for Continuing Education, University of Petroleum and Energy Studies, Dehradun, Uttarakhand.

    Aparna Singh
    Aparna Singh

    Dr. Aparna Singh (1989-1993)

    Obtained PhD in 1993. Worked on structural changes in sperm membranes during epididymal maturation, capacitation and acrosome reaction. After completing PhD, joined as a Scientist with Indian Army. Currently, working as Deputy Registrar at Centre for Continuing Education, University of Petroleum and Energy Studies, Dehradun, Uttarakhand.

    Dr. Sumita Sinha (1990-1994)

    Obtained PhD in 1994. Worked on biophysical and topographical aspects of spermatozoa from fertile and infertile human males. After completing PhD, she joined Bose Institute, Calcutta as a Post-Doctoral Associate. Moved to Cleveland Clinic Foundation in 1996. Research Scientist at Massachusetts General Hospital from 2003.

    Sumita Sinha
    Sumita Sinha

    Dr. Sumita Sinha (1990-1994)

    Obtained PhD in 1994. Worked on biophysical and topographical aspects of spermatozoa from fertile and infertile human males. After completing PhD, she joined Bose Institute, Calcutta as a Post-Doctoral Associate. Moved to Cleveland Clinic Foundation in 1996. Research Scientist at Massachusetts General Hospital from 2003.

    Dr. Suvro Chatterjee (1994-1999)

    Obtained PhD in 1994. Worked on molecular motion in sperm membranes in relation to free radical stress. WOOD-Whealan IUBMB Fellow at Hyogo Medical School, Japan; UNESCO Fellow at Max Plank Institute of Biophysics, Frankfurt; Post-Doctoral Fellow at McGill University, Montreal (1998-2001); Research Fellow at Mayo Clinic Rochester (2001-2004); Scientist, Anna University-KBC Research Centre (2004-present). Leading a group working on vascular biology. Link to http://bio.au-kbc.org/faculty/suvro/

    Suvro Chatterjee
    Suvro Chatterjee

    Dr. Suvro Chatterjee (1994-1999)

    Obtained PhD in 1994. Worked on molecular motion in sperm membranes in relation to free radical stress. WOOD-Whealan IUBMB Fellow at Hyogo Medical School, Japan; UNESCO Fellow at Max Plank Institute of Biophysics, Frankfurt; Post-Doctoral Fellow at McGill University, Montreal (1998-2001); Research Fellow at Mayo Clinic Rochester (2001-2004); Scientist, Anna University-KBC Research Centre (2004-present). Leading a group working on vascular biology. Link to http://bio.au-kbc.org/faculty/suvro/

    Dr. Prem Prakash Singh (1990-1995)

    Awarded PhD under co-mentorship of Dr. Anand Kar. Lecturer, Vikaram University Ujjain (1995-1997); Assistant Professor at Govt College, Bhopal (1997). Post-doctoral fellow at East Tennessee State University. Assistant Professor at Govt. College, Balrampur, Chhattisgarh (Present)

    prem prakash
    prem prakash

    Dr. Prem Prakash Singh (1990-1995)

    Awarded PhD under co-mentorship of Dr. Anand Kar. Lecturer, Vikaram University Ujjain (1995-1997); Assistant Professor at Govt College, Bhopal (1997). Post-doctoral fellow at East Tennessee State University. Assistant Professor at Govt. College, Balrampur, Chhattisgarh (Present)

    Dr. Sharad Purohit (1994-2000)

    Awarded PhD in 2000. Post Doctoral Fellow at McGill University, Montreal (2000-2001); Post-Doctoral Fellow at University of Florida (2001-2002); Post-Doctoral Fellow at Medial College of Georgia (2002-2012). Assistant Professor at Georgia Regents University, Augusta (2012-present). http://www.cbgm.gru.edu/FacultyandStaff/DrSPurohit.aspx

    Sharad Purohit
    Sharad Purohit

    Dr. Sharad Purohit (1994-2000)

    Awarded PhD in 2000. Post Doctoral Fellow at McGill University, Montreal (2000-2001); Post-Doctoral Fellow at University of Florida (2001-2002); Post-Doctoral Fellow at Medial College of Georgia (2002-2012). Assistant Professor at Georgia Regents University, Augusta (2012-present). http://www.cbgm.gru.edu/FacultyandStaff/DrSPurohit.aspx

    Dr. Deeksha Saxena (1997-2002)

    Obtained PhD in 2002. Post-doctoral fellow at University of Pittsburgh (2003-2013). Research Scientist at University of Pennsylvania (2013- present)

    deeksha
    deeksha

    Dr. Deeksha Saxena (1997-2002)

    Obtained PhD in 2002. Post-doctoral fellow at University of Pittsburgh (2003-2013). Research Scientist at University of Pennsylvania (2013- present)

    Dr. Kumar Chandrakuntal

    Obtained PhD in 2004. Post-Doctoral Fellow at Department of Pharmacology and Toxicology and Dept. of Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences (2005-2007). Post-doctoral fellow at University of Pittsburgh Cancer Center (2007-2011). Post-doctoral fellow at Memorial Health University Medical Center, Savannah, Georgia (2011-present).

    Kumar Chandrakuntal
    Kumar Chandrakuntal

    Dr. Kumar Chandrakuntal

    Obtained PhD in 2004. Post-Doctoral Fellow at Department of Pharmacology and Toxicology and Dept. of Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences (2005-2007). Post-doctoral fellow at University of Pittsburgh Cancer Center (2007-2011). Post-doctoral fellow at Memorial Health University Medical Center, Savannah, Georgia (2011-present).

    Dr. Mopidevi Brahmaraju (2002-2007)

    Obtained PhD in 2007. Postdoctoral Fellow, Department of Pathology, New York Medical College, Valhalla, NY (2009-2011); Postdoctoral Fellow, Department of Physiology and Pharmacology, University of Toledo, Toledo, OH (2011-2013); Assistant Professor, Department of Physiology and Pharmacology, University of Toledo, Toledo, OH (2013-present) https://www.utoledo.edu/med/depts/physpharm/faculty/Mopidevi.html

    Mopidevi Brahmaraju
    Mopidevi Brahmaraju

    Dr. Mopidevi Brahmaraju (2002-2007)

    Obtained PhD in 2007. Postdoctoral Fellow, Department of Pathology, New York Medical College, Valhalla, NY (2009-2011); Postdoctoral Fellow, Department of Physiology and Pharmacology, University of Toledo, Toledo, OH (2011-2013); Assistant Professor, Department of Physiology and Pharmacology, University of Toledo, Toledo, OH (2013-present) https://www.utoledo.edu/med/depts/physpharm/faculty/Mopidevi.html

    Dr. Mohammed Shoeb (2002-2010)

    Obtained PhD in 2010. Studied the organization and functional role of membrane raft micro-domains in spermatozoa. Post-doctoral fellow at the cardiovascular biology division, Oklahoma medical research foundation, Oklahoma city (2011-2012) and worked on the molecular mechanism of membrane raft targeting of cell adhesion receptor CD44 using FRET. Post-doctoral fellow at the Medical college of Wisconsin, Milwaukee (2012-2013) and worked on understanding the role of GPER-1 in pancreatic beta cells. Presently, working at Shri Shankaracharya College, Bhilai as Assistant professor (Biotechnology).

    Mohammed Shoeb
    Mohammed Shoeb

    Dr. Mohammed Shoeb (2002-2010)

    Obtained PhD in 2010. Studied the organization and functional role of membrane raft micro-domains in spermatozoa. Post-doctoral fellow at the cardiovascular biology division, Oklahoma medical research foundation, Oklahoma city (2011-2012) and worked on the molecular mechanism of membrane raft targeting of cell adhesion receptor CD44 using FRET. Post-doctoral fellow at the Medical college of Wisconsin, Milwaukee (2012-2013) and worked on understanding the role of GPER-1 in pancreatic beta cells. Presently, working at Shri Shankaracharya College, Bhilai as Assistant professor (Biotechnology).

    Dr. Ambika Chandrasekhar (2004-2011)

    Awarded PhD in 2011. Worked on the activation and inactivation of NOX system in maturing mammalian spermatozoa and its relevance to sperm functioning. Post-doctoral fellow at University of Calgary, Canada (2012-2014). Post-doctoral fellow in University of Alberta (2014-present) with special focus on diabetic neuropathy.

    Ambika Chandrasekhar
    Ambika Chandrasekhar

    Dr. Ambika Chandrasekhar (2004-2011)

    Awarded PhD in 2011. Worked on the activation and inactivation of NOX system in maturing mammalian spermatozoa and its relevance to sperm functioning. Post-doctoral fellow at University of Calgary, Canada (2012-2014). Post-doctoral fellow in University of Alberta (2014-present) with special focus on diabetic neuropathy.

    Dr. Uma Chandran (2005-2012)

    Obtained PhD in 2012. Explored the expression of Cnnm1 in spermatogenice cells and its role in regulating cell cycle in germ cells. Currently working as Post doctoral fellow on 'Exploring Ayurvedic Medicinal Plants for Phosphodiesterase Inhibitory Activity' under the Mentorship of Dr. Bhushan Patwardhan at Interdisciplinary School of Health Sciences at Savitribai Phule Pune University.

    Uma Chandran
    Uma Chandran

    Dr. Uma Chandran (2005-2012)

    Obtained PhD in 2012. Explored the expression of Cnnm1 in spermatogenice cells and its role in regulating cell cycle in germ cells. Currently working as Post doctoral fellow on 'Exploring Ayurvedic Medicinal Plants for Phosphodiesterase Inhibitory Activity' under the Mentorship of Dr. Bhushan Patwardhan at Interdisciplinary School of Health Sciences at Savitribai Phule Pune University.

    Dr. Indu S (2005-2012)

    Obtained PhD in 2013. Isolated, characterized and expanded germline stem cells from neonatal and adult testis. Evaluated the expression profile of several stemness determinants in GSCs and produced embryoid body-like clusters in culture. Post-doctoral fellow at Sree Chitra Tirunal Institute for Medical Science and Technology, Thiruvananthapuram (2012-2013).

    Indu S
    Indu S

    Dr. Indu S (2005-2012)

    Obtained PhD in 2013. Isolated, characterized and expanded germline stem cells from neonatal and adult testis. Evaluated the expression profile of several stemness determinants in GSCs and produced embryoid body-like clusters in culture. Post-doctoral fellow at Sree Chitra Tirunal Institute for Medical Science and Technology, Thiruvananthapuram (2012-2013).

    Dr. Sreesha Sree (2008-2013)

    Awarded PhD in 2016. Worked on miRNA-mRNA networks in mouse testis during the initiation of spermatogenesis.

    sreesa
    sreesa

    Dr. Sreesha Sree (2008-2013)

    Awarded PhD in 2016. Worked on miRNA-mRNA networks in mouse testis during the initiation of spermatogenesis.

    Dr. Divya Saro Varghese (2008-2013)

    Awarded PhD in 2016. Worked on the functional analysis of Tar DNA Binding Protein 43 (TDP43) in germ cell development in testis. Currently working as a researcher at United Arab Emirates University, College of Medicine and Health SCiences, Department of Biochemistry, Al Ain, Abu Dhabi , United Arab Emirates. https://www.researchgate.net/profile/Divya_Varghese

    divya sara
    divya sara

    Dr. Divya Saro Varghese (2008-2013)

    Awarded PhD in 2016. Worked on the functional analysis of Tar DNA Binding Protein 43 (TDP43) in germ cell development in testis. Currently working as a researcher at United Arab Emirates University, College of Medicine and Health SCiences, Department of Biochemistry, Al Ain, Abu Dhabi , United Arab Emirates. https://www.researchgate.net/profile/Divya_Varghese

    Dr. Bhagya K P

    Awarded PhD in 2016. Worked on the functional aspects of Autoimmune regulator (AIRE) in germ cell division and differentiation in testis.

    divya sara
    divya sara

    Dr. Divya Saro Varghese (2008-2013)

    Awarded PhD in 2016. Worked on the functional analysis of Tar DNA Binding Protein 43 (TDP43) in germ cell development in testis. Currently working as a researcher at United Arab Emirates University, College of Medicine and Health SCiences, Department of Biochemistry, Al Ain, Abu Dhabi , United Arab Emirates. https://www.researchgate.net/profile/Divya_Varghese

    Project Fellows

    Sanjeeva Srivastava (1999-2000)

    Project fellow in a DBT-sponsored research grant. Ph.D. University of Alberta, Canada (Nov-2006); Post-doc, Harvard Institute of Proteomics, Harvard Medical School, USA (2007-2009); Assistant Professor, Bioscience and Bioengineering, IIT Mumbai (2009-2014); Associate Professor, Bioscience and Bioengineering, IIT Mumbai (2014-present).
    http://www.bio.iitb.ac.in/people/faculty/srivastava-s

    Sanjeeva Srivastava
    Sanjeeva Srivastava

    Sanjeeva Srivastava (1999-2000)

    Project fellow in a DBT-sponsored research grant. Ph.D. University of Alberta, Canada (Nov-2006); Post-doc, Harvard Institute of Proteomics, Harvard Medical School, USA (2007-2009); Assistant Professor, Bioscience and Bioengineering, IIT Mumbai (2009-2014); Associate Professor, Bioscience and Bioengineering, IIT Mumbai (2014-present).
    http://www.bio.iitb.ac.in/people/faculty/srivastava-s

    Meena Balani (2000-2002)

    Teacher Fellow from a Government College in Madhya Pradesh. Currently working as Research Manager in Orwig Research Group at Magee-Womens Research Institute and Foundation, University of Pittsburgh Medical Centre, Pittsburgh, PA.
    http://www.mwrif.org/202

    Meena Balani
    Meena Balani

    Meena Balani (2000-2002)

    Teacher Fellow from a Government College in Madhya Pradesh. Currently working as Research Manager in Orwig Research Group at Magee-Womens Research Institute and Foundation, University of Pittsburgh Medical Centre, Pittsburgh, PA.
    http://www.mwrif.org/202

    Padma Priya (2001)

    Project Fellow in a DBT-sponsored research grant.

    Padma Priya
    Padma Priya

    Padma Priya (2001)

    Project Fellow in a DBT-sponsored research grant.

    Pooja Gupta (2001)

    Project Fellow in a DBT-sponsored research Grant.

    Pooja Gupta (2001)

    Project Fellow in a DBT-sponsored research Grant.

    Sumit Sethi (2002-2005)

    Project Fellow in a UGC-sponsored research grant. PhD from Department of Zoology, Banaras Hindu University. Working as a Lecturer at Monash University, Sunway Campus, Malaysia.
    http://my.linkedin.com/pub/dr-sumit-sethi/a/b93/749

    Sumit Sethi
    Sumit Sethi

    Sumit Sethi (2002-2005)

    Project Fellow in a UGC-sponsored research grant. PhD from Department of Zoology, Banaras Hindu University. Working as a Lecturer at Monash University, Sunway Campus, Malaysia.
    http://my.linkedin.com/pub/dr-sumit-sethi/a/b93/749

    Abha Palta (2003-2004)

    Project Fellow in a DBT-sponsored research grant.

    Abha Rege
    Abha Rege

    Abha Palta (2003-2004)

    Project Fellow in a DBT-sponsored research grant.

    Srimanti Guhathakurta (2003-2005)

    Project Fellow in a UGC-sponsored research grant.

    Srimanti Guhathakurta
    Srimanti Guhathakurta

    Srimanti Guhathakurta (2003-2005)

    Project Fellow in a UGC-sponsored research grant.

    Subir Biswas (2004-2005)

    Project fellow in a DST-sponsored research grant. PhD from Jawaharlal Nehru University, New Delhi. Post Doctoral fello w at University of North Carolina at Chapel Hill, NC and at University of Texas at Austin. Post Doctoral Fellow at IISER, Bhopal. Associate Scientist at Lupin Pharmaceuticals, Pune.
    http://in.linkedin.com/pub/subir-biswas-phd/17/572/5

    Subir Biswas
    Subir Biswas

    Subir Biswas (2004-2005)

    Project fellow in a DST-sponsored research grant. PhD from Jawaharlal Nehru University, New Delhi. Post Doctoral fello w at University of North Carolina at Chapel Hill, NC and at University of Texas at Austin. Post Doctoral Fellow at IISER, Bhopal. Associate Scientist at Lupin Pharmaceuticals, Pune.
    http://in.linkedin.com/pub/subir-biswas-phd/17/572/5

    Neena Mary Thomas (2006-2007)

    Project Fellow in a DST-sponsored research grant. Currently working as a Research Specialist in Nussenzweig Lab at the Rockefeller University, New York.
    http://www.rockefeller.edu/research/faculty/labmembers/MichelNussenzweig/

    Neena Mary Thomas
    Neena Mary Thomas

    Neena Mary Thomas (2006-2007)

    Project Fellow in a DST-sponsored research grant. Currently working as a Research Specialist in Nussenzweig Lab at the Rockefeller University, New York.
    http://www.rockefeller.edu/research/faculty/labmembers/MichelNussenzweig/

    Sreeja C Sekhar (2006-2009)

    Project Fellow in a BRNS-sponsored research grant. Obtained PhD from Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan. http://www.pubfacts.com/author/Sreeja+C+Sekhar

    Sreeja C. Sekhar
    Sreeja C. Sekhar

    Sreeja C Sekhar (2006-2009)

    Project Fellow in a BRNS-sponsored research grant. Obtained PhD from Department of Biotechnology, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan. http://www.pubfacts.com/author/Sreeja+C+Sekhar

    Anoop Chandran (2006)

    Project Fellow in a DBT-sponsored research grant.

    Anoop Chandran
    Anoop Chandran

    Anoop Chandran (2006)

    Project Fellow in a DBT-sponsored research grant.

    Tara Chandran (2006-2009)

    Project Fellow in a DBT-sponsored research grant. Currently working for PhD at Sree Chitra Tirunal Institute of Medical Science and Technology, Thiruvananthapuram.

    Tara Chandran
    Tara Chandran

    Tara Chandran (2006-2009)

    Project Fellow in a DBT-sponsored research grant. Currently working for PhD at Sree Chitra Tirunal Institute of Medical Science and Technology, Thiruvananthapuram.

    Nima Mary George (2007-2008)

    Project fellow in a DST-sponsored research grant.

    Nima Mary George
    Nima Mary George

    Nima Mary George (2007-2008)

    Project fellow in a DST-sponsored research grant.

    Varsha R Prasad (2008-2010)

    Project Fellow in a DST-sponsored research grant. Currently working for PhD at School of Life Sciences, University of Hyderabad.

    Varsha R. Prasad
    Varsha R. Prasad

    Varsha R Prasad (2008-2010)

    Project Fellow in a DST-sponsored research grant. Currently working for PhD at School of Life Sciences, University of Hyderabad.

    Ann Mary Joseph (2009-2010)

    Project Fellow in a KSCSTE-sponsored research grant. Currently working for PhD at School of Biotechnology, Jawaharlal Nehru University, New Delhi.

    Ann Mary Joseph
    Ann Mary Joseph

    Ann Mary Joseph (2009-2010)

    Project Fellow in a KSCSTE-sponsored research grant. Currently working for PhD at School of Biotechnology, Jawaharlal Nehru University, New Delhi.

    Chitra JS (2010)

    Project Fellow in a DBT-sponsored research grant. Currently working for PhD with Dr. Asha S. Nair at RGCB.
    http://localhost:8080/workspace/wordpress/scientist/s-asha-nair/

    Chitra JS
    Chitra JS

    Chitra JS (2010)

    Project Fellow in a DBT-sponsored research grant. Currently working for PhD with Dr. Asha S. Nair at RGCB.
    http://localhost:8080/workspace/wordpress/scientist/s-asha-nair/

    Suja Stephen (2011-2013)

    Project Fellow in a DBT-sponsored research grant.

    Suja Stephen
    Suja Stephen

    Suja Stephen (2011-2013)

    Project Fellow in a DBT-sponsored research grant.

    Nithya Sekhar (2012-2013)

    Project Fellow in a BRNS-sponsored research grant.

    Nithya Sekhar
    Nithya Sekhar

    Nithya Sekhar (2012-2013)

    Project Fellow in a BRNS-sponsored research grant.

    Chinnumol VS (2013)

    Project Fellow in a DBT-sponsored research grant.

    Chinnumol VS
    Chinnumol VS

    Chinnumol VS (2013)

    Project Fellow in a DBT-sponsored research grant.