Profile

Research

Publications

Team

Alumni

Radhakrishnan R, PhD

Scientist E-I

+91-471-2781222

radhakrishnan@rgcb.res.in

radhakrishnan-r
  • Profile

    • PhD, Biochemistry (Regional Cancer Centre, University of Kerala)
    • MSc, Medical Biochemistry (Mangalore University, Karnataka)
    • October 2011- till date: Scientist EI, Laboratory Medicine and Molecular Diagnosis
    • January 2011 - October 2011: Dean, Professor of Biochemistry, Consultant Laboratory Medicine, Atlantic University NY, USA
    • October 2009 - January 2011: Professor and Chair of Biochemistry Spartan University NY, USA
    • September 2007 - November 2009: Associate Professor Biochemistry, Consultant at the collegiate hospital and visiting consultant, Sultan Quaboos University Oman Medical College, West Virginia University, Muscat, Oman
    • November 2004 - August 2007: Associate Professor Biochemistry, Head division of Laboratory Medicine, MOSC Medical College, Kolencherry, Ernakulam Kerala India
    • May 2004 - November 2004: Associate ProfessorHead Division of Laboratory Medicine Manipal College of Medical Sciences, Pokahra Nepal
    • April 2002 - May 2004: Associate Professor, Dept. of Biochemistry, Consultant & Head Division of Laboratory Medicine & Molecular Biology, IbnSina Teaching Hospital, Al Tahadi University, Sirte, Libya
  • Research

    Cancer Genomics

    LMMD offers molecular tests that provide diagnostic information, predict sensitivity to chemotherapeutic agents and aid in the management of patients with neoplastic disorders. It is now possible to phenotype and genotype human tumours to increase the accuracy and reproducibility of pathologic diagnosis. Neonatal screening using next generation sequencing, for intrauterine genetic aberrations suggestive of paediatric neoplasm risk is a major on-going research program.

    Cardiovascular Genomics

    These services use evaluation of genotype as a tool for prediction of disease risk and drug response. The mechanisms of cardiac arrhythmias in a number of conditions including ischaemic heart disease, Brugada syndrome, Arrhythmogenic Right Ventricular Cardiomyopathy and Long QT Syndrome are analyzed by identifying genes responsible for causing sudden cardiac death, identifying markers of risk in specific patient groups and providing evidence for optimising prevention & treatment strategies.

    Pharmacogenomics

    LMMD collaborates with transplantation medicine physicians who need the services of gene sequencing. This work is carried out to understand the mechanisms linking genetic variation with drug response, based on the premise that strength of genetic associations with risk factors that lie in the causal pathway should be concordant with observed genetic associations with drug dosage end-points. Personalized drug dosage and regime determination for transplant medicine is thus a major highlight of this laboratory service.

    Infectious diseases

    LMMD provides rapid-turnaround diagnostic virology for more than 30 viruses of clinical importance. The full range of tests provided can be found online at www.rgcb.res.in/lmmd. Virus detection is mainly carried out using polymerase chain reaction (PCR)-based assays, several of which are multiplexed as well as real time PCR tests. All PCR assays used in the laboratory have undergone extensive validation by QCMD of United Kingdom and incorporate all needed internal controls.

    Inherited diseases

    Hemoglobinopathies testing and genetic counseling: The primary objective of this target is to provide recommendations to physicians, midwives and genetic counselors involved in pre-conceptional or prenatal care regarding carrier screening for thalassemia and hemoglobinopathies (e.g., sickle cell anemia and other qualitative hemoglobin disorders).Screening of individuals at increased risk of being carriers for thalassemia and hemoglobinopathies can identify couples with a risk of having a pregnancy with a significant genetic disorder for which prenatal diagnosis is possible. This would include a complete blood count as well as hemoglobin electrophoresis or HPLC, abnormal hemoglobin and HbF quantitation,and H body staining and genetic analysis.

    Testing platform development

    Molecular diagnostics has evolved dramatically over the past 15 years providing benefits of quicker turnaround times, improved sensitivities and increased accuracy. These new techniques have greatly improved the detection of many viral and bacterial infections, cancer, cardiovascular disease as well as genetic disorders. Technology drives this industry. The global molecular diagnostics market is expected to reach US$5.8 billion by 2015, according to a report by Global Industry Analysts, Inc. New technologies, innovations and expanded applications for tests have been fueling steady growth in the field. The market for molecular diagnostics is very broad because it is not limited to one field of study. Molecular techniques are utilized in genetics, infectious disease, oncology, hematology and pharmacology. While blood screening and infectious disease contribute to the majority of the market; DNA sequencing is a big factor in the growth of molecular diagnostics. The emerging technology of personalized medicine relies on DNA sequencing and molecular diagnostics. Personalized genome sequencing helps determine the best approach to patient care, whether it is preventive, diagnostic or therapeutic

    • LMMD has developed and externally validated in field trials a Lateral Flow device for testing Classical Swine Fever (CSFV) antibody.
    • LMMD has established for the first time in the world, CSFV antigen based lateral flow testing device that is currently being validated.
    • LMMD has developed and validated LAMP based assay for Dengue virus testing.
    • LMMD is in the final stages of development of a lateral flow based device that simultaneously diagnoses disease and provides drug sensitivity for chronic myleoid leukemia (CML)
    1. Pharmacogenomic analysis of Cyp3 mutations to calculate optimal tracolimus drug dosage in renal transplant recipients. LMMD- Nephrology Dept. Govt. Medical College, Thiruvananthapuram.
    2. Genomic profiling of patients with hypertrophic cardiomyopathy LMMD- Cardiology Dept. KIMS Hospital.
    3. Viral Conjunctivitis a clinical study, LMMD- Regional Institute of Ophthalmology, Govt. Medical College Thiruvananthapuram.
    4. LMMD has signed MOU with Collegiate hospital of Gov. Medical College, KIMS Hospital and PRS Hospital for collaboration in clinical research. Clinical samples additionally comes from Govt. Health services departments like the PHCs, SK Hospital and Cosmopolitan Hospital.
    5. Individual projects between departments.

    Commercial kit development is one among the mandates of LMMD. The need for rapid point-of-care testing has become a necessity of the day. Keeping this in mind, several kits are being developed at LMMD. Classical Swine Fever Virus (CSFV) lateral flow device was devised to alleviate the absence of trained Vets and support personnel in the Far Eastern regions of India. The Ab kit detects CSFV from any body fluid. An Antigen based CSFV Lateral Flow kit was developed for the first time in the world. Identified Dengue serotype 4 after a long gap of 20 years in the Country.

  • Publications

    Publications (2013-2015)

    1. Ramakrishnan L, Pillai MR, Nair RR Dengue vaccine development: strategies and challenges. Viral Immunol. 2015 Mar;28(2):76-84. doi: 10.1089/vim.2014.0093. Epub 2014 Dec 10.
    2. Detection of measles virus genotype B3 in India. Kuttiatt VS, Kalpathodi S, Gangadharan ST, Kailas L, Sreekumar E, Sukumaran SM, Nair RR. Emerging Infectious Diseases, 2014 Volume 20, (Number 10), 1765-1756
    3. Comparative performance of the probable case definitions of dengue by WHO (2009) and the WHO-SEAR expert group (2011) Zinia T. Nujum, Achu Thomas, K. Vijayakumar, Radhakrishnan R. Nair, M. Radhakrishna Pillai, P S Indu, Syam Sundar, Soumya Gopakumar, Devi Mohan, T. K. Sudheeshkumar. Pathogens and Global Health 2014, 108, Issue 2, pp. 103-110
    4. Con?rmation of self-reported non-smoking status by salivary cotinine among diabetes patients in Kerala, India G.K. Mini, Mark Nichter, Radhakrishnan R. Nair, K.R. Thankappan. Clinical Epidemiology and Global Health (2014) online pp 1-3
    5. Seven Genebank®accession numbers KJ938501 through KJ938507 awarded for Dengue 4.
  • Team


    Sanjai D, Senior Manager (Technical Services)

    I am mainly taking care of all technical operations related to all the projects of the LMMD and maintaining the instruments related molecular diagnostics, their quality checks in the instrumentation facility at LMMD. Apart from supporting quality management, also involved in developing new molecular diagnostics platforms like LAMP assays, undertaking research frontiers like immunome analysis in different infectious diseases

    sanjai
    sanjai

    Sanjai D, Senior Manager (Technical Services)

    I am mainly taking care of all technical operations related to all the projects of the LMMD and maintaining the instruments related molecular diagnostics, their quality checks in the instrumentation facility at LMMD. Apart from supporting quality management, also involved in developing new molecular diagnostics platforms like LAMP assays, undertaking research frontiers like immunome analysis in different infectious diseases

    Dr. Dayakar, Research Associate

    I did my Ph.D in Human Genetics from Osmania University, Hyderabad. before joining RGCB as a Research Associate in LMMD; I have been working with Kamuzu central Hospital association with Howard University as a Laboratory scientist, Malawi, Africa (1year) and Apollo Hospitals, Hyderabad working as a Research Scientist in Molecular biology and cytogenetic lab (8years). My area of interest focus on detection and analysis of nucleic acid sequences, estimation of viral loads, molecular diagnosis of cancer and other diseases. Constitutional cytogenetic anomalies in postnatal and acquired cytogenetic anomalies in cancer.

    Dayakar
    Dayakar

    Dr. Dayakar, Research Associate

    I did my Ph.D in Human Genetics from Osmania University, Hyderabad. before joining RGCB as a Research Associate in LMMD; I have been working with Kamuzu central Hospital association with Howard University as a Laboratory scientist, Malawi, Africa (1year) and Apollo Hospitals, Hyderabad working as a Research Scientist in Molecular biology and cytogenetic lab (8years). My area of interest focus on detection and analysis of nucleic acid sequences, estimation of viral loads, molecular diagnosis of cancer and other diseases. Constitutional cytogenetic anomalies in postnatal and acquired cytogenetic anomalies in cancer.

    Jayalekshmi D, MSc

    Senior Research Fellow

    My work involves study of the frequency and genotype specificity of HPV infection among vaccinated and unvaccinated women in the 2 versus 3 dose HPV vaccine trial in India. We use a high throughput multiplex Luminex technology based assay system. I am also involved as part of a study funded by European Union in investigating the relation between site of tumor in the oro-pharynx and HPV virus genotype.

    Jayalekshmi-D
    Jayalekshmi-D

    Jayalekshmi D, MSc

    Senior Research Fellow

    My work involves study of the frequency and genotype specificity of HPV infection among vaccinated and unvaccinated women in the 2 versus 3 dose HPV vaccine trial in India. We use a high throughput multiplex Luminex technology based assay system. I am also involved as part of a study funded by European Union in investigating the relation between site of tumor in the oro-pharynx and HPV virus genotype.

    Kannan T R, MSc

    Technical Assistant

    I work for the IARC funded project "Accurate and satisfactory analysis of all high risk HPV types and some low risk type antibody titers for the 2 verses 3 dose HPV vaccination clinical trial in India." I provide technical support for both virus genotyping and serological analysis of the specimens in this study.

    Kannan-TR
    Kannan-TR

    Kannan T R, MSc

    Technical Assistant

    I work for the IARC funded project "Accurate and satisfactory analysis of all high risk HPV types and some low risk type antibody titers for the 2 verses 3 dose HPV vaccination clinical trial in India." I provide technical support for both virus genotyping and serological analysis of the specimens in this study.

    Sreeja S, Lab Technician

    I am involved in processing & analyzing biological specimens & performing molecular biology techniques such as sample aliquotion , DNA/RNA analysis, PCR analysis, agarose gel electrophoresis, gel band purification ,sequencing PCR, sequencing purification , genotyping analysis & serological techniques like ELISA.

    Sreeja
    Sreeja

    Sreeja S, Lab Technician

    I am involved in processing & analyzing biological specimens & performing molecular biology techniques such as sample aliquotion , DNA/RNA analysis, PCR analysis, agarose gel electrophoresis, gel band purification ,sequencing PCR, sequencing purification , genotyping analysis & serological techniques like ELISA.

    Karthika V, Research Assistant

    My work includes registration of samples in the software and finalizing invoice and billing. Submitting monthly, quarterly and annual reports to ICMR. Filing of patients request forms, work sheets, registers, temperature log sheets, indents, CRV forms & consumables data sheets. Generating indents. Conducting Purchase Committee meetings and sending purchase orders after receiving and comparing the quotations from different companies. Receiving the consumables from stores and maintaining the sub stocks provided. Updating the chemicals indented and received till date. Assistance in the administration work including issuing certificates, settling of advances, updating fund details.

    Karthika
    Karthika

    Karthika V, Research Assistant

    My work includes registration of samples in the software and finalizing invoice and billing. Submitting monthly, quarterly and annual reports to ICMR. Filing of patients request forms, work sheets, registers, temperature log sheets, indents, CRV forms & consumables data sheets. Generating indents. Conducting Purchase Committee meetings and sending purchase orders after receiving and comparing the quotations from different companies. Receiving the consumables from stores and maintaining the sub stocks provided. Updating the chemicals indented and received till date. Assistance in the administration work including issuing certificates, settling of advances, updating fund details.

    Akhila Suresh, Project Assistant

    I am involved in aliquotion of various patient samples, isolation of RNA, DNA & ELISA.

    Akhila
    Akhila

    Akhila Suresh, Project Assistant

    I am involved in aliquotion of various patient samples, isolation of RNA, DNA & ELISA.

    Vineetha, Lab Technician

    My work includes centrifugation of blood samples, sample aliquotion, storage, extraction of nucleic acid either DNA,RNA,PCR, gel electrophoresis, verification of results, gel band purification, sequencing and identifying the genotypes, ELISA etc

    Vineetha
    Vineetha

    Vineetha, Lab Technician

    My work includes centrifugation of blood samples, sample aliquotion, storage, extraction of nucleic acid either DNA,RNA,PCR, gel electrophoresis, verification of results, gel band purification, sequencing and identifying the genotypes, ELISA etc

    Sumaja V, Helper

    My work includes cleaning of instruments and working area. Waste disposal in biohazard bags, refilling hypochloride solution in the working area, filling of tip in the tip boxes, arranging the eppendorfs and tip boxes for autoclaving. Receiving consumables and non-consumables from stores. Assisting the technicians and updating the temperature of freezers.

    Sumaja
    Sumaja

    Sumaja V, Helper

    My work includes cleaning of instruments and working area. Waste disposal in biohazard bags, refilling hypochloride solution in the working area, filling of tip in the tip boxes, arranging the eppendorfs and tip boxes for autoclaving. Receiving consumables and non-consumables from stores. Assisting the technicians and updating the temperature of freezers.

  • Alumni