Profile

Research

Publications

Team

Alumni

Ruby John Anto, PhD, FNASc

Scientist F

+91-471-2529473

rjanto@rgcb.res.in

ruby john anto1
ruby john anto1

Ruby John Anto, PhD, FNASc

Scientist F

+91-471-2529473

rjanto@rgcb.res.in

  • Profile

    • Ph.D Biochemistry (Amala Cancer Research Centre, Thrissur) – 1995
    • M.Ed Physical Science (Govt. Training College, Thrissur) – 1989
    • B.Ed Physical Science (Govt. Training College, Thrissur) – 1987
    • M.Sc Chemistry (Govt College, Madappally) – 1985
    • B.Sc Chemistry (Little Flower College, Guruvayur) – 1983
    • July 2009- till date, Scientist E-II, Rajiv Gandhi Centre for Biotechnology
    • July 2004- July 2009, Scientist E-I, Rajiv Gandhi Centre for Biotechnology
    • April 2000 July 2004, Scientist C, Rajiv Gandhi Centre for Biotechnology
    • June 2000- June 2001, Visiting Scientist, Dept. of Bioimmunotherapy, M.D. Anderson Cancer Center, Houston, Texas, USA
    • September 1996 April 2000, Research Associate, Rajiv Gandhi Centre for Biotechnology

    Awards

    • Lekshmi.R.Nath, Ravishanker Lankalapalli and Ruby John Anto.; Best paper Award under Biotechnology category at  28th Kerala Science Congress, University of Calicut Campus, Thenhipalam, Malappuram District, India, January 28-30, 2016
    • Lekshmi.R.Nath, Sophia Margaret Joseph, Nikhil . P. Anto ,Sai Shyam Narayanan, Smitha .V. Bava and Ruby John Anto 4-O-Methylgallic acid, isolated from the  leaves of the mistletoe growing on Saraca asoca induces caspase-mediated apoptosis in cervical cancer cells via down-regulation of HPV. Best Poster Award at the 8th Eurobiotechnology congress, Frankfurt,Germany, August 17-20,2015
    • Lekshmi.R.Nath, Awarded ICMR and CICS International Travel grants in 2015 for participating the 8th Eurobiotechnology congress, Frankfurt , Germany. August 17-20, 2015
    • 2015: Minakshi Saikia was awarded  JSPS HOPE fellowship to participate in the 7th HOPE meeting with Nobel laureates, conducted by the Japan Society for Promotion of Science (JSPS) held at Tokyo (March 1-5, 2015).
    • 2015: Arun Kumar T Thulasidasan, Devika Nandan C, Lekha Nair K, J Jisha Pillai, G.S.Vinod Kumar and Ruby John Anto. Best oral presentation award at National Conference on Challenges and Future Prospects of Applied Research in Life Sciences-2015, Bharathidasan University, Tiruchirappalli.
    • 2015: Ruby John Anto. Excellence award for outstanding performance, RGCB, Promotion assessment committee.
    • 2014: Haritha H Nair, Balachandran S Vinod, Jayesh Antony, Minakshi Saikia and Ruby John Anto Best Poster Award at 33
    • 2012: Haritha H Nair, Balachandran S Vinod, Jayesh Antony, Minakshi Saikia and Ruby John Anto Best speaker award & Best Poster Award at 3rd International Conference on Carcinogenesis, New Delhi
    • 2012: Jayesh Antony, Sophia Margaret Joseph, Lekshmi R Nath, Harsha Chandran and Ruby John Anto, Best Poster Award at International Conference on Advances In Biological Sciences , Kannur.
    • 2010: Jayesh Antony, Sophia Margaret Joseph, Lekshmi R Nath, Harsha Chandran and Ruby John Anto, Best Poster Award at The International Cancer Research Symposium-, Thiruvananthapuram
    • 2008: Sreekanth C.N and RJ Anto, Best Poster Award at International Society Biotechnology Conference, Gangtok, Sikkim.
    • 2004: National Woman Bio-Scientist Award, DBT, Govt of India.
    • 1995: Fr. Gabriel Golden Jubilee Award for best paper: Amala Cancer Research Centre, Thrissur.

    Fellowships

    • Fellow, National Academy of Sciences, Allahabad
    • Member, Editorial board – PLOS ONE
    • Member, Editorial board- Asian Journal of Pharmacological Sciences
    • Member, American Society For Biochemistry And Molecular Biology
    • Member, The Biotech Research Society
    • Member, Board of Studies, M.Sc Chemistry, University of Kerala (2004-07)
    • Member, Board of Studies, MSc Biotechnology, University of Calicut (2007-2010)

    Funding Agencies

    • Department of Science and Technology
    • Council of Scientific and Industrial Research
    • Indian Council of Medical Research

    Journals

    • Antioxid Redox Sign
    • Apoptosis
    • Plos One
    • Int. J. Cancer
    • J. Cellular and Mol. Med.
    • Cell Death &Dis
    • BMC Cancer
    • Immunopharmacol and Immunotoxicol
    • Drug Delivery
    • Evidence Based Complementary &Alternative Med
    • Free Rad Res
    • J.Stem Cells
    • Therapeutic Delivery
    • Int J Pharm
    • Intl J Biochem & Cell Biol

    Awarded

    • Ruby John Anto, A chemotherapeutic composition used in the treatment of cancer: Filing Number: #1184/Del/2004. IPR- Patent number: 241416, Date of grant-02/07/2010: Funding by DBT, Govt. of India

    Filed

    • Ruby John Anto and Lakshmi R Nath, Uttroside B and derivatives thereof as therapeutics for hepatocellular carcinoma, Filed on 28 May 2016 Patent Application No. 201641018401. Funding by Rajiv Gandhi Centre For Biotechnology.
    • Ruby John Anto and Jayesh Antony, Extracts from a plant, processes for obtaining the extracts and fractions and applications thereof, Filed on: 20.09.2010, Patent Application No. 2743/CHE/2010: Funding by RGCB
    • C Mohandas, Bala Nambisan, Ruby John Anto, Vino T. Cheriyan and S. Nishanth Kumar. Anticancer activity of a cyclic dipeptide isolated from novel bacteria associated with Rhabditis sp., Filed on: 11-03-2011 Patent Application No. 820/CHE/2011). Funding by Indian Council for Agricultural Research
  • Research

    Our lab is mainly focusing on identification of nontoxic chemosensitizers, which can be used along with the conventional chemotherapeutic drugs used for cancer treatment so that the cost and toxicity due to chemotherapy can be minimized. Our in vitro and in vivo studies have clearly proved that curcumin can be used as an effective chemosensitizer in paclitaxel chemotherapy against cervical cancer (Bava et al, 2005, Bava et al, 2011, Sreekanth et al, 2011). Multistage squamous cell carcinoma was induced in the uterine cervix of Swiss albino mice using 3-methylcholanthrene to evaluate the synergistic anticancer effect of paclitaxel and curcumin. The synergism was also elucidated in a xenograft tumor model of human cervical cancer in NOD-SCID mice. The results proves that curcumin augments the chemotherapeutic efficacy of paclitaxel by down-regulating the survival signals such as NF-?B, Akt and MAPKs that play key roles in proliferation, survival, angiogenesis and metastasis.

    Our recent studies using breast cancer cells indicate that curcumin can also be used effectively to chemosensitize breast cancer cells to 5-FU, independent of their receptor status, through TS- dependent down-regulation of NF-?B, which was confirmed by silencing TS and inactivating NF-?B, both of which reduced the chemosensitizing efficacy of curcumin. Silencing of TS suppressed 5-FU-induced NF-?B activation, while inactivation of NF- ?B did not affect 5-FU-induced TS up-regulation, confirming that TS is upstream of NF-?B and regulates the activation of NF-?B in 5-FU-induced signaling pathway. Akt/PI3K pathway and MAPK pathway do not have any role in regulating the synergism, though both these pathways are activated by 5-FU and down-regulated by curcumin (Vinod et al, 2013B). In vivo studies to validate the efficacy of the combination are in progress. An invited review has also been published recently from our lab on the clinical relevance of chemosensitization, giving special emphasize to phenolic phytochemicals (Vinod et al, 2013A).

    We are also exploring non toxic chemopreventives, which can inhibit environmental carcinogenesis. We observed that nicotine induces a proliferative tendency in lung cancer cells, especially in those lacking p53. While very low concentrations of nicotine induced activation of various survival signals in p53 null cells, almost 100 times higher concentration of nicotine was required to induce the same effect in cells with active p53. The response of these cells to nicotine was reversed when their p53 status was reversed. However, curcumin down-regulated these survival signals independent of the p53 status of the cells (Vineshkumar et al, 2010). Our study reveals a detrimental role to nicotine especially in lung cancer patients with impaired p53 status and identifies curcumin as a potential chemo preventive. In vivo studies also show that curcumin can inhibit the lung carcinogenesis induced by Benzo [a] pyrene. Currently we are investigating whether nano encapsulation can improve the bioavailability and retention time of curcumin within the body so that its chemopreventive efficacy can be improved.

    Recently, we have also set foot into another area of research, which is bioprospecting for novel anticancer drugs from the vast biodiversity of Kerala. In a collaborative study, we found out that a complex protein fraction isolated from the plant, Sesbania grandiflora can prevent ascites and solid tumor development and reverse the survival signals induced by PMA in colon cancer cells (Laladhas et al, 2010). We isolated a semipurified fraction from the leaves of the plant, Wrightia tinctoria, which is inducing strong cytotoxicity towards melanoma cells while being non-toxic to human epidermal melanocytes (Patent Application No. 2743/CHE/2010). We also isolated tryptanthrin from these leaves, which is the first detailed report of this compound from this plant. We observed that this compound also is very effective against melanoma cells while being non-toxic to normal melanocytes. Studies to evaluate the in vivo efficacy of this compound as a chemopreventive/ chemotherapeutic/ chemosensitizer are in programme.

    Ph.D Produced

    1. Dr. Smitha V Bava 2008 (Title: Potential role of curcumin, the color pigment of turmeric in sensitizing conventional chemotherapeutic drugs)
    2. Dr. Vinesh Kumar TP 2009 (Title: Regulatory role of curcumin on the carcinogenic effect induced by ingredients of cigarette smoke)
    3. Dr. Sreekanth CN 2011 (Title: Evaluation of the synergistic effect of paclitaxel and curcumin in carcinogen-induced and xenograft models of cervical carcinogenesis in mice)
    4. Dr. Vinod BS 2014 (Title: Identification of effective and non-toxic chemosensitizers, which can be used in combination with the conventional chemotherapeutic drugs used for breast cancer treatment)
    5. Dr. Jayesh Antony 2015 (Title: Molecular evaluation of anticancer properties of active principle's from Wrightia tinctoria)
    6. Dr. Arun Kumar T Thulasidasan 2015 (Nanoparticle-based drug delivery and drug release for chemotherapy and chemoprevention)
    Sl. No Title of Project Funding Agency Duration From -To Total Approved Cost of the Project(Rs.) Output Publications/Patets
    1 Mechanistic evaluation and in vivo validation of the anticancer principle isolated from Chromolaena odorata against cervical cancer KSCSTE 2016-2019 23,89,400/-
    2 Isolation, identification and characterization of anticancer principle from the medicinal plant Corallocarpus epigaeus (Co-PI) KSCSTE 2016-2019 30,60,400/-
    3 Investigating the mechanism behind the protective effect of the anticancer compounds isolated from Woodfordia fruticosa(L.)Kurz flowers against hepatocellular carcinoma (Co-PI) KSCSTE 2016-2019 Money not received

    Completed Research Projects

    Sl. No Title of Project Funding Agency Duration From-To Total Approved Cost of the Project(In Rs.) Output Publications/Patets
    1 Synergistic effect of Taxol and curcumin in inducing apoptosis of cancer cells STEC, Govt. of Kerala 2003-2006 6,07,000 Bava et al 2005, J. Biol. Chem., 280: 6301 - 6308
    2 Sensitization of conventional chemotherapeutic drugs using non-toxic chemo preventives DBT, Govt. of India 2003-2008 Program support to RGCB 1. Bava et al 2005, J. Biol. Chem., 280: 6301 - 6308
    2. Bava et al 2010, Int J Biochem Cell Biol, 43, 331-341.
    3. Sreekanth et al, 2011, Oncogene. 30, 3139-3152, 2011.
    4. Vinod et al 2013, Cell Death and Disease 4,e505; doi:10.1038/cddis.2013.26,2013
    5. Vinod et al 2013, Antioxidants & Redox Signaling. 18, 1307-1348, 2013.
    6. Vinod et al 2015, Cell Death Discovery,1,15061; doi:10.1038/cddiscovery.2015.61, 2015.
    3 Potential Role of curcumin in regulating the effects induced by the major carcinogens in cigarette smoke, such as nicotine, NNK and B(a)P in normal and cancerous lung cells. DST, Govt. of India STNo:SR/SO/HS-49/2002 2005-2008 19,97,000 1. Puliyappadamba et al 2010, Mol Cancer, Highly Accessed. 9, 220.
    2. Puliyappadamba et al.2015, BioFactors, doi: 10.1002/biof.1244., 2015
    4 Isolation and identification of anticancer principle from Mistletoes(Viscum album) growing on various host trees ICMR, Govt. of India 2006-2009 10,17,600 Lekshmi.R.Nath, et al, Best Poster Award at the 8th Eurobiotechnology congress, Frankfurt , Germany, August 17-20,2015
    5 Development of High Specific and High Affinity monoclonal antibody against human IgM. TreeLand Biotech (Industrial project) 2006-2009 32,38,320/- Developed monoclonal antibody against human IgM
    6 Isolation and identification of anticancer principle from the indigenous medicinal plant, Wrightia tinctoria CSIR, Govt. of India 2006-2009 12,72600/- Jayesh Antony et al, Scientific Reports, 5:11107, DOI: 10.1038/srep11107, 2015
    7 Antineoplastic molecules associated with bacteria from entomopathogenic nematodes Co-PI STEC, Govt of Kerala 2007-2010 11,25300/- 1. Patent Filed on: 11-03-2011 Patent Application No. 820/CHE/2011)
    2. Lekshmi.R.Nath, et al, Frontiers in Microbiology, 7:452, doi: 10.3389/fmicb.2016.00452, 2016
    8 Novel polymer nanoparticle based drug releasing systems for improving the efficacy of drug administration in cancer chemotherapy( Co-PI) DBT, Govt. of India 2008-2011 41,53,000/- 1. Lekha et al, 2012, Int J Pharm. 425(1-2):44-52.
    2. Deepa et al, 2012, Int J. Nanomedicine,7, 4077 - 4088.
    3. Pillai et al, 2014, J Nanobiotechnology. 12: 25;
    4. Pillai et al, 2015, RSC Adv.,5, 25518-25524. DOI:10.1039/C5RA00018A.
    9 Comparison of the chemopreventive efficacy of free curcumin and biodegradable polymer based nano curcumin in Benzo[a] pyrene-induced lung carcinogenesis DST, Govt. of India 2013-2016 51,56,200/- Puliyappadamba et al.2015, BioFactors, DOI: 10.1002/biof.1244., 2015
    10 Isolation and identification of anticancer principle from the mistletoe growing on Chrsophyllum spp CSIR 2012-2015 23,55,400/- Manuscript under preparation
    11 In vivo evaluation of the anticarcinogenic effect and toxicity of the active principle of Wrightia tinctoria ICMR, Govt. of India 2015-2016 6,37,916/- Standardized skin carcinogenesis model
    1. Organizing secretary, International Cancer Research Symposium-2010- Defining and Translating Science Behind the Disease, 20-22nd December 2010, Rajiv Gandhi Center for Biotechnology, Thiruvananthapuram, Kerala.
    1. Dr. G.S.Vinod Kumar, Division of Chemical Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram.
    2. Dr.M.Sreerama Moorthy, Indian Institute of Chemical technology, Hyderabad Identification of new of anticancer drugs
    3. Dr. Ravi Shankar L, National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram
  • Publications

    Publications

    1.  Lekshmi R. Nath, S. N. Kumar, Arya A. Das, Bala Nambisan, A. Shabna, Chellapan Mohandas* and Ruby John Anto*: In vitro evaluation of the antioxidant, 3,5-Dihydroxy-4-ethyl-trans-stilbene (DETS) isolated from Bacillus cereus as a potent candidate against malignant melanoma. Frontiers in Microbiology, 7:452,   doi: 10.3389/fmicb.2016.00452, 2016.
    2. Smitha V Bava, Arun Kumar T Thulasidasan, Sreekanth CN and *Ruby John Anto, Cervical cancer: A comprehensive approach towards extermination. Annals of Medicine, DOI: 10.3109/07853890.2016.1145796, 2016 Cervical cancer: A comprehensive approach towards extermination.
    3. Vinod BS, Haritha Nair, Vinod V, Shabna A, Shabna S, Archana K, Kavya SP, Sanu T and *Ruby John Anto: Resveratrol chemosensitizes HER-2-over-expressing breast cancer cells to docetaxel chemoresistance by inhibiting docetaxel-mediated activation of HER-2-Akt axis. Cell Death Discovery, 1, 15061; doi:10.1038/cddiscovery.2015.61, 2015.
    4. Vineshkumar TP, Arun Kumar TT, Vinod V, Jayesh A, Smitha VB, Shabna A, Sankar S and *Ruby John Anto: Curcumin inhibits B[a]PDE-induced pro-carcinogenic signals in lung cancer cells, and curbs B[a]P-induced mutagenesis and lung carcinogenesis. BioFactors. 41(6):431-42.DOI:10.1002/biof.1244,2015.
    5. Lekshmi R. Nath, Jaggaiah N. Gorantla, Sophia Margaret Joseph, Jayesh Antony, Sanu Thankachan, Darsan B. Menon, S. Sankar, Ravi S. Lankalapalli and *Ruby John Anto.:Kaempferide, the most active among the four flavonoids isolated and characterized from Chromolaena odorata, induces apoptosis in cervical cancer cells while being pharmacologically safe. RSC Adv., 2015, 5, 100912.
    6. Jayesh Antony, Minakshi Saikia, Vino T. Cheriyan, Nishanth Kumar S and *Ruby John Anto. Sesbania: A Prospective Candidate to be Excavated for Anticancer Drugs. The Natural Products Journal, 2015, 5(4): 273-287, DOI: 10.2174/1872211310999151110155644.
    7. Haritha H Nair, *Ruby John Anto. Triple negative breast cancer: The therapeutic windows yet to be opened? Science Jet , 4:175, 2015
    8. Jayesh Antony, Minakshi Saikia, Vinod.V, Lekshmi. R. Nath, Mohana Rao Katiki, M.S.R. Murty, Anju Paul, Shabna A, Harsha Chandran, Sophia Margaret Joseph, Nishanth Kumar. S, Elizabeth Jayex Panakkal, Sriramya I.V, Sridivya I.V, Sophia Ran, Sankar S, Easwary Rajan and *Ruby John Anto. DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria, Sci. Reports. In press. Published online 5:11107|DOI:10.1038/srep11107, 2015.
    9. Jisha J. Pillai, Arun Kumar T. Thulasidasan, Ruby John Anto, Nandan C. Devika, N. Ashwanikumar and G. S. Vinod Kumar. Curcumin entrapped folic acid conjugated PLGA–PEG nanoparticles exhibit enhanced anticancer activity by site specific delivery, RSC Adv.,5, 25518-25524. DOI: 10.1039/C5RA00018A. 2015.
    10. Radhakrishnan E K, Smitha V Bava, Sai Shyam Narayanan, Lekshmi R Nath, Arun Kumar Thulasidasan, Soniya E V and *Ruby John Anto. [6]-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancer cells by inhibiting MAPK/AP-1 signaling, PLOS ONE, 9(8): e104401. doi:10.1371, 2014.
    11. Jisha Jayadevan Pillai, Arun Kumar Theralikattu Thulasidasan, Ruby John Anto, Devika Nandan Chithralekha, Ashwanikumar Narayanan, and Gopalakrishnapillai Sankaramangalam Vinod Kumar. Folic acid conjugated cross-linked acrylic polymer (FA-CLAP) hydrogel for site specific delivery of hydrophobic drugs to cancer cells, J Nanobiotech.. 12: 25; 2014.
    12. Sasidharan Nishanth Kumar, Sreerag Ravikumar Sreekala, Dileep Chandrasekaran, Bala Nambisan and Ruby John Anto. Biocontrol of Aspergillus Species on peanut kernels by antifungal diketopiperazine producing Bacillus cereus associated with entomopathogenic nematode. PLOS ONE, 9(8): e106041. doi:10.1371, 2014.
    13. M. Sreelekha, NP Anto, Ruby John Anto and PM Shafi. Cytotoxicity of acetonyl dihydro chelerythrin,arnottianamide and 6-(2.hydroxy propyl)-dihydro chelerythrine towards human cancer cell lines , Ind. J. Chem. Sec.B, 647-651, 2014.
    14. Jaggaiah N. Gorantla, Jamsheena Vellekkatt, Lekshmi R.Nath, Ruby John Anto and Ravi S. Lankalapalli. Cytotoxicity studies of semi-synthetic derivatives of theveside derived from the aqueous extract of leaves of‘suicide tree’ Cerbera odollam, Natural Product Res, DOI: 10.1080/14786419.2014.913242, 2014.
    15. Kumar SN, Nambisan Bala, Sundaresan A, Mohandas C and Ruby John Anto, Isolation and identification of antimicrobial secondary metabolites from Bacillus cereus associated with a rhabditid entomopathogenic nematode. Ann Microbiol., 64, 209–218, 2014.
    16. M. S. R. Murty, Raju Penthala, Lekshmi R. Nath and Ruby John Anto, Synthesis of Salicylic Acid-Based 1, 3, 4-Oxadiazole Derivatives Coupled with Chiral Oxazolidinones: Novel Hybrid Heterocycles as Antitumor Agents Letters in Drug Design & Discovery, 11, 1-10, 10.2174/1570180811666140627004607, 2014.
    17. Kumar SN, Nambisan B, Kumar BS, Vasudevan NG, Mohandas C, Cheriyan VT and Ruby John Anto, Antioxidant and anticancer activity of 3,5-dihydroxy-4-isopropylstilbene produced by Bacillus sp. N strain isolated from entomopathogenic nematode. Arch Pharm Res. DOI: 10.1007/s12272-013-0207-2. 2013 [Epub ahead of print].
    18. P.Jyothi, T.Shalina Begum, UCA.Jaleel, N.P.Anto, Ruby John Anto and PM.Shafi, Synthesis and biological evaluation of some novel imidazolinones, Int J Pharm Biomed Sci, 2013, 4(2), 78-82
    19. BS Vinod, J Antony, HH Nair, VT Puliyappadamba, M Saikia, S Shyam Narayanan, A Bevin and *Ruby John Anto. Mechanistic evaluation of the signaling events regulating curcumin-mediated chemosensitization of breast cancer cells to 5-fluorouracil. Cell Death Dis 4, e 505; doi:10.1038/cddis.2013.26, 2013.
    20. Balachandran S Vinod, Tessy T Maliekal and *Ruby John Anto, Phytochemical As Chemosensitizers: From Molecular Mechanism to Clinical Significance. Comprehensive Invited Review. Antioxid. Redox Signal. 18, 1307–1348, 2013.
    21. M. S. R. Murty, B. Ramalingeswara Rao, Mohana Rao Katiki, Lekshmi R. Nath & Ruby John Anto, Synthesis of piperazinyl benzothiazole/benzoxazole derivatives coupled with 1,3,4 oxadiazole-2-thiol: novel hybrid heterocycles as anticancer agents. Med Chem Res., 22, 4980-4991, 2013.
    22. Deepa G, Arun Kumar T Thulasidasan, Ruby John Anto, J Jisha Pillai, GS Vinod Kumar. Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy. Int J. Nanomedicine,7, 4077 – 4088, 2012.
    23. Lekha Nair K, Arun Kumar T Thulasidasan, Deepa G, Ruby John Anto, GS Vinod Kumar. Purely aqueous PLGA nanoparticulate formulations of curcumin exhibit enhanced anticancer activity with dependence on the combination of the carrier. Int J Pharm.4:425 (1-2), 44-52, 2012.
    24. M. S. R. Murty, B. Ramalingeswara Rao, Kesur R. Ram, J. S. Yadav, Jayesh Antony and Ruby John Anto. Synthesis and preliminary evaluation activity studies of novel 4-(aryl/heteroaryl-2-ylmethyl)-6-phenyl-2-[3-(4-substituted-piperazine-1-yl)propyl]pyridazin-3(2H)-one derivatives as anticancer agents. Med Chem Res. 21, 3161-3169 2011.
    25. P. L. Anto, Ruby John Anto, Hema Tresa Varghese, C. Yohannan Panicker, Daizy Philip, Gustavo F.S. Andrade, Alexandre G. Brolo. Spectroscopic investigations and computational study of sulfur trioxide–pyridine complex. J. Raman Spectrosc. 42, 1812–1819, 2011.
    26. Chanickal N Sreekanth, Smitha V Bava, E Sreekumar and *Ruby John Anto. Molecular evidences for the chemosensitizing efficacy of liposomal curcumin in paclitaxel chemotherapy in mouse models of cervical cancer. Oncogene. 30, 3139-3152, 2011.
    27. M.S.R. Murty, Kesur R. Ram, Rayudu Venkateswara Rao, J.S. Yadav, Janapala Venkateswara Rao, , Vino. T. Cheriyan and Ruby John Anto. Synthesis and preliminary evaluation of 2-substituted-1,3- benzoxazole and 3-[(3-substituted)propyl]-1,3-benzoxazol- 2(3H)-one derivatives as potent anticancer agents. Med Chem Res. 20:576–586, 2011.
    28. Smitha V Bava, Chanickal N Sreekanth, Arun Kumar T Thulasidasan, Nikhil P Anto, Vino T Cheriyan, Vineshkumar T Puliyappadamba, Sajna G Menon, Santhosh D Ravichandran, and *Ruby John Anto. Akt is upstream and MAPKs are downstream of NF-κB in paclitaxel-induced survival signaling events, which are down-regulated by curcumin contributing to their synergism. Int J Biochem Cell Biol. 43, 331-341, 2011.
    29. Vineshkumar T Puliyappadamba, Vino T Cheriyan, Arun Kumar T Thulasidasan, Smitha V Bava, Balachandran S Vinod, Priya R Prabhu, Ranji Varghese, Arathy Bevin, Shalini Venugopal and *Ruby John Anto. Nicotine-induced survival signaling in lung cancer cells is dependent on their p53 status while its down-regulation by curcumin is independent. Mol Cancer, 9, 220, 2010. (Highly Accessed).
    30. Krishna P Laladhas, Vino T Cheriyan, Vineshkumar T Puliappadamba, Smitha V Bava, Rajesh G Unnithan, Parvathy L Vijayammal and *Ruby John Anto. A novel protein fraction from Sesbania grandiflora induces apoptosis in cancer cells and shows potential anticancer and chemopreventive efficacy in vivo. J Cellular and Mol. Med. 14, 636-646, 2010.
    31. Sanalkumar R, Indulekha C L , Divya T S, Divya M S , Ruby John Anto, Vinod B, Vidyanand S, Jagatha B, Shalini V and Jackson James. ATF2 maintains a subset of neural progenitors through CBF1/Notch independent Hes-1 expression and synergistically activates the expression of Hes-1 in Notch dependent neural progenitors. J. Neurochem. 113, 807-818, 2010.
    32. M. D. Ajitha Bai , D. P. N. Nair , T. L. Gayathri , K. P. Padmakumaryamma, Ruby John Anto, G. Jayakumar , Ajaikumar and R. Rajasree. Chemical composition and bioactivities of volatile components from the seed and bark oils of Litsea coriaceae Hook. f., Lauraceae. International Journal of Essential Oil Therapeutics 4, 1-4, 2010.
    33. P. L. Anto , Ruby John Anto, HT Varghese, C Y Panicker and Daizy Philip. Vibrational spectroscopic studies and ab initio calculations of phenyl phosphate disodium salt. J. Raman Spectrosc. 41, 113-119, 2010.
    34. Rajendran G, Amritha C S, Ruby John Anto and Vino T Cheriyan. Synthesis, thermal and antitumor studies of thorium (IV) complexes with furan-2- aldehyde-N-phenyl thiosemicarbazone. J. Serb. Chem. Soc. 75, 1-13, 2010.
    35. P. L. Anto, Ruby John Anto, H. T. Varghese, C. Y. Panicker, D. Philip, and A. G. Brolo. (2009) FT-IR, FT-Raman and SERS spectra of Anilinium Sulfate: J. Raman Spectrosc., 40,1810-15
    36. Manickam Venkatraman, Ruby John Anto, Asha Nair, Merina Varghese and Devarajan Karunagaran (2005) Biological and chemical inhibitors of NF-κB sensitize SiHa cells to cisplatin-induced apoptosis: Mol. Carcinogenesis, 44:51-59.
    37. Smitha V. Bava, Vineshkumar T. Puliappadamba, Ayswaria Deepti, Asha Nair, Devarajan Karunagaran, and *Ruby John Anto (2005) Sensitization of taxol-induced apoptosis by curcumin involves down regulation of NF-?B and Akt and is independent of tubulin polymerization: J. Biol. Chem., 280: 6301 – 6308.
    38. Tessy Thomas Maliekal, Ruby John Anto and D. Karunagaran (2004) Differential activation of Smads in HeLa and SiHa cells that differ in their response to transforming growth factor-β: J. Bio. Chem., 279: 36287 – 92.
    39. Suby Oommen, Ruby John Anto, Srinivas G and Karunagaran D. (2004) Allicin (from garlic) induces caspase- mediated apoptosis in cancer cells: European. J. Pharmacol., 485,97-103.
    40. Srinivas G, Ruby John Anto, Priya Srinivas, S Vidhyalaksmi, V Priya Senan and D Karunagaran D. (2003) Emodin induces apoptosis of human cervical cancer cells through poly (ADP-ribose) polymerase cleavage and activation of caspases-9: European.J.Pharmacol., 473, 117-25.
    41. Ruby John Anto, Manickam Venkatraman and Devarajan Karunagaran (2003) Inhibition of N F-κB sensitizes A431 Cells to Epidermal Growth Factor- induced Apoptosis whereas its activation by ectopic expression of RelA confers resistance: J. Biol.Chem., 278, 25490-98.
    42. Ruby John Anto, Asok Mukhopadyay, Sisir Sishodia, Gary C. Gyrola and Bharat Aggarwal. (2002) Cigarette Smoke Condensate Activates Nuclear Transcription Factor-?B Through Phosphorylation and Degradation of IκBα: Its Role in Induction of Cyclooxygenase-2: Carcinogenesis, 23, 1511-18.
    43. Ruby John Anto, Asok Mukhopadyay, Kate Denning and Bharat B. Aggarwal (2002) Curcumin (Diferuloylmethane) induces apoptosis through activation of caspase-8, BID cleavage and cytochrome C Release: Its Suppression by ectopic expression of Bcl-2 and Bcl-xL: Carcinogenesis, 23, 143-150./li>
    44. Ruby John Anto, Tessy T Maliekal and Devarajan Karunagaran (2000) L-929 Cells Harboring Ectopically Expressed Rel A Resist Curcumin- induced Apoptosis: J. Biol Chem., 275, 15601-604.
    45. Ruby John Anto, Girija Kuttan, Dinesh Babu KV, Rajasekharan KN and Kuttan R.(1998) Anti-inflammatory activity of natural and synthetic curcuminoids: Pharm. Pharmacol. Commun. , 4, 103-106.
    46. Ruby John Anto, Josely George, Dinesh Babu KV, Rajasekharan KN and Kuttan R.(1996) Antimutagenic and anticarcinogenic activities of natural and synthetic curcuminoids: Mutation Res., 370, 127- 131
    47. Ruby John Anto, Girija Kuttan, Dinesh Babu KV, Rajasekharan KN and Kuttan R.(1996) Synthetic curcuminoids as anticancer agents and as free radical scavangers: Int. J. Pharm., 131, 1-8.
    48. Ruby John Anto, Girija Kuttan, M.N. A. Rao, V. Subbaraju and Ramadasan Kuttan (1995) Anticancer and antioxidant activity of synthetic chalcones and related compounds: Cancer Letters, 97, 33-37
    49. Ruby John Anto, Girija Kuttan, Dinesh Babu KV, Rajasekharan KN and Kuttan R. (1995) Antitumor and antioxidant activity of curcuminoids: Cancer Letters, 94, 79-83.
    50. Ruby John Anto, Girija Kuttan, Ramadasan Kuttan, Kota Satyanarayana and M.N. A. Rao, (1994) Tumor reducing and antioxidant activities of sydnone substituted chalcones: J. Clin. Biochem.and Nutr., 17, 73- 80
    1. Lekshmi.R.Nath, Ravi Shankar L and *Ruby John Anto. In vitro and in vivo evaluation of the anticancer potential of SN2, a spirostan-3-ol derivative identified and characterized from Solanum nigrum against hepatocellular carcinoma., Proceedings of 28th Kerala Science Congress, Page: no: 330-341, 2016
    2. Shabna A and *Ruby John Anto. Role of MITF in melanoma. Amala Research Bulletin 35(2015),Pp 24-31
    3. Mohan Shankar G and *Ruby John Anto. Role of Hedgehog signaling in basal cell carcinoma. Amala Research Bulletin (2014) 34, 115-121
    4. Minakshi Saikia and *Ruby John Anto. Acute myeloid leukemia: Causes, diagnosis, classification and treatment modalities. Amala Research Bulletin (2013) 33, 147-154.
    5. Haritha H Nair and *Ruby John Anto. Triple negative breast cancer: The unusual one among the many. Amala Research Bulletin (2012) 32, 100-108.
    6. Arun Kumar TT and *Ruby John Anto. Nanotechnology and its applications in the field of medicine. Amala Research Bulletin (2011) 31, 144-154.
    7. Lekshmi. R Nath and Ruby John Anto. General approaches towards extraction of medicinal plants. Amala Research Bulletin (2010)30, 150-158.
    8. Deepa I, Vino T Cheriyan and *Ruby John Anto. An overview of protein kinase B/Akt signaling in cancer. Amala Research Bulletin (2009) 29, 19-26.
    9. Jayesh Antony and *Ruby John Anto (2008). A molecular approach to skin cancer. Amala Research Bulletin, 28, 141-152.
    10. Priya R Prabhu and *Ruby John Anto (2007) Molecular pathogenesis of colorectal cancer. Amala Research Bulletin, 27, 212-230.
    11. Vinod BS and *Ruby John Anto (2006) Human Epidermal Growth factor receptor signaling as a therapeutic target in breast cancer Amala Research Bulletin, 26, 18-27.
    12. CN Sreekanth  and *Ruby John Anto (2005) Cervical cancer -The risk factors and Treatment modalities. Amala Research Bulletin, 25, 81-92.
    13. Smitha Bava, Vineshkumar TP and *Ruby John Anto (2005). Molecular mechanism behind the synergistic effect of Taxol and curcumin. Proceedings of 17th Kerala Science Congress75-77.
    14. Vineshkumar.TP, Smitha Bava and *Ruby John Anto (2005) Curcumin down-regulates Nicotine resistance in lung cancer cells. Indian Journal of Medical Resc. (supplement), 100
    15. Vineshkumar.TP and *Ruby John Anto (2004) Cigarette Smoking and Lung Cancer: Environmental and Molecular Implications. Amala Research Bullettin, 24, 31-41
    16. Smitha VB and *Ruby John Anto (2003) Antiapototic proteins as targets of chemotherapy. Amala Research Bullettin, 23, 1-6.
    17. *Ruby John Anto, Karunagaran D and Smitha VB (2003) Curcumin potentiates taxol induced apoptosis of cervical cancer cells: Proceedings of Kerala Science Congress, 15, 263- 268.

    *Corresponding author

    Book Chapter

    1. #G Mohan Shankar, #Jayesh Antony, *Ruby John Anto- Quercetin and Tryptanthrin:Two Broad Spectrum Anticancer Agents for Future Chemotherapeutic Interventions, In: ENZ 37, Mechanism of the anticancer effect of phytochemicals. Fuyu Tamanoi (Ed)  Chapter Number:1, Pubd. Elsevier Inc., June 2015 ISSN 1874-6047. http://dx.doi.org/10.1016/bs.enz.2015.05.001, (#equal authorship)
    2. #Jayesh Antony, #Minakshi Saikia and *Ruby John Anto. Phytochemicals from Fruits and Vegetables as potential anti-cancer agents: special reference to skin cancer. In: Anticancer properties of fruits and vegetables: A scientific review. Ajay Kunnummakkara (Ed). Pubd: World Scientific Publishing Co.; 2014 edition, pp 277-307 (#equal authorship)
    3. CN Sreekanth, Smitha VB, Arun Kumar TT, N P Anto, VT Cheriyan, Vineshkumar TP, SG Menon, SD Ravichandran and *Ruby John Anto – Curcumin: A Potent Candidate to be Evaluated as a Chemosensitizer in Paclitaxel Chemotherapy Against Cervical Cancer. In: Perspectives in Cancer Prevention-Translational Cancer Research. P Sudhakaran P, Oommen V, Pillai, MR (Eds.). Pubd: Springer; 2014 edition.pp 21-43.
    4. *Ruby John Anto, Smitha V.B., Vinesh Kumar T.P., Sreekanth C.N., Vinod B.S., Vino T. Cherian, Priya R Prabhu and Gayathri L.T. Phenolics of the functional food – “Curcumin- A nutraceuticals with potential chemosensitive  property”, Role of dietary fibers and nutraceuticals in preventing diseases, Pubd -Pharma Med Press. 2008. p-202-208.
  • Team


    Dr. Archana P R, Post Doctoral Fellow

    Synthesis of carrier encapsulated phytochemical formulations and evaluation of their efficacy in animal models

    In the present scenario of cancer drug resistance, phytochemicals are potential therapeutic options as chemotherapeutics as well as chemosensitizers. However, the therapeutic outcome of most of the phytochemicals is hindered by their hydrophobicity and poor bioavailability, which lead to their reduced absorption in vivo. Encapsulation of these molecules in rationally designed drug delivery carriers offer several pharmacological benefits. My research focuses on synthesizing drug delivery carriers for various phytochemical formulations and studying their efficacy in various animal models.

    lekshmi
    lekshmi

    Dr. Archana P R, Post Doctoral Fellow

    Synthesis of carrier encapsulated phytochemical formulations and evaluation of their efficacy in animal models

    In the present scenario of cancer drug resistance, phytochemicals are potential therapeutic options as chemotherapeutics as well as chemosensitizers. However, the therapeutic outcome of most of the phytochemicals is hindered by their hydrophobicity and poor bioavailability, which lead to their reduced absorption in vivo. Encapsulation of these molecules in rationally designed drug delivery carriers offer several pharmacological benefits. My research focuses on synthesizing drug delivery carriers for various phytochemical formulations and studying their efficacy in various animal models.

    Ms. Lekshmi R Nath, SRF

    Isolation & Identification of Anti-Cancer Principles from Plants

    Natural products in general and medicinal plants in particular, are believed to be an important source of new chemical substances with potential therapeutic efficacy. Utilization of potential medicinal plants with application of sophisticated assays and scientific research methodologies can help in the identification of new effective drugs. My present interest lies in identifying novel anticancer principles with potential activity from plants. It involves studying the cytotoxic effect of crude extracts from selected plants in cancer cells followed by purification of the biologically active component from the crude extracts; explore the anticancer effect of the active principle and the signal transduction pathways regulating its anticancer activity.

    lekshmi
    lekshmi

    Ms. Lekshmi R Nath, SRF

    Isolation & Identification of Anti-Cancer Principles from Plants

    Natural products in general and medicinal plants in particular, are believed to be an important source of new chemical substances with potential therapeutic efficacy. Utilization of potential medicinal plants with application of sophisticated assays and scientific research methodologies can help in the identification of new effective drugs. My present interest lies in identifying novel anticancer principles with potential activity from plants. It involves studying the cytotoxic effect of crude extracts from selected plants in cancer cells followed by purification of the biologically active component from the crude extracts; explore the anticancer effect of the active principle and the signal transduction pathways regulating its anticancer activity.

    Ms. Haritha H Nair, PhD student (SRF)

    In vivo validation of synergistic combinations of conventional chemotherapeutic drugs for breast cancer treatment with non-toxic chemosensitizers

    Previous in vitro studies conducted in the lab using different breast cancer cells has found out certain synergistic combinations of conventional chemotherapeutic drugs and non-toxic chemosensitizers. It had been found that some of these combinations are having promising anti tumor activity with minimum side effects. Before using these combinations in clinical trials, their efficacy must be validated through animal experiments. The present study aims at the in vivo evaluation of these combinations and elucidation of the signaling pathways underlying the antitumor activity of these combinations.

    haritha
    haritha

    Ms. Haritha H Nair, PhD student (SRF)

    In vivo validation of synergistic combinations of conventional chemotherapeutic drugs for breast cancer treatment with non-toxic chemosensitizers

    Previous in vitro studies conducted in the lab using different breast cancer cells has found out certain synergistic combinations of conventional chemotherapeutic drugs and non-toxic chemosensitizers. It had been found that some of these combinations are having promising anti tumor activity with minimum side effects. Before using these combinations in clinical trials, their efficacy must be validated through animal experiments. The present study aims at the in vivo evaluation of these combinations and elucidation of the signaling pathways underlying the antitumor activity of these combinations.

    Ms. Minakshi Saikia, PhD student (SRF)

    Identification of effective chemosensitizers for Leukemia and Melanoma chemotherapy

    Development of chemoresistance is one of the major drawbacks of cancer chemotherapy. The need for better drugs is the call of the hour to combat the problem. Several compounds extracted from natural sources have been shown to be beneficial in sensitizing the cancer cells towards conventional drugs, so that the dose of the chemotherapeutics can be reduced, thereby minimizing the side effects. The present study is intended to identify natural non-toxic compounds, which can chemosensitize leukemia and melanoma cells.

    minakshi
    minakshi

    Ms. Minakshi Saikia, PhD student (SRF)

    Identification of effective chemosensitizers for Leukemia and Melanoma chemotherapy

    Development of chemoresistance is one of the major drawbacks of cancer chemotherapy. The need for better drugs is the call of the hour to combat the problem. Several compounds extracted from natural sources have been shown to be beneficial in sensitizing the cancer cells towards conventional drugs, so that the dose of the chemotherapeutics can be reduced, thereby minimizing the side effects. The present study is intended to identify natural non-toxic compounds, which can chemosensitize leukemia and melanoma cells.

    Mohan Shanker, PhD student (SRF)

    Evaluation of the chemopreventive efficacy of the active principle from Wrightia tinctoria against skin carcinogenesis

    The need to find an effective chemopreventive agent is gaining more importance since we are frequently exposed to carcinogens and incidence of different types of cancer is always ascending. We have isolated an anticancer principle from Wrightia tinctoria which is very effective against melanoma. I am focusing on evaluating the chemopreventive efficacy of this active principle against skin cancer using a well established carcinogenesis model. We hope that the work will contribute to the introduction of a new potent chemopreventive agent sans side effects that usually accompany usage of existing treatment regimens.

    minakshi
    minakshi

    Mohan Shanker, PhD student (SRF)

    Evaluation of the chemopreventive efficacy of the active principle from Wrightia tinctoria against skin carcinogenesis

    The need to find an effective chemopreventive agent is gaining more importance since we are frequently exposed to carcinogens and incidence of different types of cancer is always ascending. We have isolated an anticancer principle from Wrightia tinctoria which is very effective against melanoma. I am focusing on evaluating the chemopreventive efficacy of this active principle against skin cancer using a well established carcinogenesis model. We hope that the work will contribute to the introduction of a new potent chemopreventive agent sans side effects that usually accompany usage of existing treatment regimens.

    Shabna A, PhD student (JRF)

    Mechanistic evaluation of the chemotherapeutic efficacy of tryptanthrin against malignant melanoma

    Melanoma is often considered as one of the most aggressive and treatment-resistant human cancer. Previous studies conducted in the laboratory has demonstrated that tryptanthrin is a potent anticancer agent against malignant melanoma, while being non-toxic to normal melanocytes and skin fibroblasts. Its biological safety has also been evaluated by in vivo toxicological studies . It was found that tryptanthrin significantly reduces melanoma tumor growth, angiogenesis and metastasis in in vivo models. My research will be focusing on unraveling the molecular mechanism and signaling events involved in regulating the therapeutic potential of tryptanthrin in melanoma chemotherapy.

    minakshi
    minakshi

    Shabna A, PhD student (JRF)

    Mechanistic evaluation of the chemotherapeutic efficacy of tryptanthrin against malignant melanoma

    Melanoma is often considered as one of the most aggressive and treatment-resistant human cancer. Previous studies conducted in the laboratory has demonstrated that tryptanthrin is a potent anticancer agent against malignant melanoma, while being non-toxic to normal melanocytes and skin fibroblasts. Its biological safety has also been evaluated by in vivo toxicological studies . It was found that tryptanthrin significantly reduces melanoma tumor growth, angiogenesis and metastasis in in vivo models. My research will be focusing on unraveling the molecular mechanism and signaling events involved in regulating the therapeutic potential of tryptanthrin in melanoma chemotherapy.

    Shabna. S, PhD student (JRF)

    Mechanistic evaluation of the anticancer potential of uttroside B against liver cancer

    Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high rate of morbidity and mortality. Previous studies conducted in our lab has proved that uttroside B, isolated from the methanolic extract of the leaves of Solanum nigrum Linn exhibits exceptional cytotoxicity towards liver cancer cells, while being non-toxic to normal hepatocytes. Uttroside B, along with the induction of apoptosis, also induces vacuole formation, a characteristic feature of autophagy in liver cancer cells. Our attempt to analyze the role of uttroside B in inducing autophagy in HepG2 cells revealed that it is an inducer of autophagy. Though our previous study has confirmed that the drug is very effective against hepatic cancer, the mechanism responsible for its anticancer potential against liver cancer is still not very clear. Hence, we are interested in studying the molecular mechanism and signaling events regulating the therapeutic potential of uttroside B.

    minakshi
    minakshi

    Shabna. S, PhD student (JRF)

    Mechanistic evaluation of the anticancer potential of uttroside B against liver cancer

    Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high rate of morbidity and mortality. Previous studies conducted in our lab has proved that uttroside B, isolated from the methanolic extract of the leaves of Solanum nigrum Linn exhibits exceptional cytotoxicity towards liver cancer cells, while being non-toxic to normal hepatocytes. Uttroside B, along with the induction of apoptosis, also induces vacuole formation, a characteristic feature of autophagy in liver cancer cells. Our attempt to analyze the role of uttroside B in inducing autophagy in HepG2 cells revealed that it is an inducer of autophagy. Though our previous study has confirmed that the drug is very effective against hepatic cancer, the mechanism responsible for its anticancer potential against liver cancer is still not very clear. Hence, we are interested in studying the molecular mechanism and signaling events regulating the therapeutic potential of uttroside B.

    Aiswarya U.S, Project fellow (JRF)

    minakshi
    minakshi

    Aiswarya U.S, Project fellow (JRF)

    Jannet. S, Research Assistant

    minakshi
    minakshi

    Jannet. S, Research Assistant

  • Alumni


    Dr. Smitha V Bava

    Assistant Professor
    University of Calicut

    alumini1
    alumini1

    Dr. Smitha V Bava

    Assistant Professor
    University of Calicut

    Dr. Vineshkumar T Puliyappadamba

    Postdoctoral Researcher
    UT Southwestern
    Dallas, USA

    alumini2
    alumini2

    Dr. Vineshkumar T Puliyappadamba

    Postdoctoral Researcher
    UT Southwestern
    Dallas, USA

    Dr. C N Sreekanth

    Postdoctoral Fellow
    University of Minnesota, USA

    alumini3
    alumini3

    Dr. C N Sreekanth

    Postdoctoral Fellow
    University of Minnesota, USA

    Dr.Vinod B S

    Assistant Professor
    SN College
    Kollam, Kerala

    alumini4
    alumini4

    Dr.Vinod B S

    Assistant Professor
    SN College
    Kollam, Kerala

    Dr. Jayesh Antony

    Awarded PhD in 2015. Worked on  molecular evaluation of anticancer properties of active principles from Wrightia tinctoria. Currently working as a assistant professor in St.Thomas College,Palai

    jayesh
    jayesh

    Dr. Jayesh Antony

    Awarded PhD in 2015. Worked on  molecular evaluation of anticancer properties of active principles from Wrightia tinctoria. Currently working as a assistant professor in St.Thomas College,Palai

    Dr. Arun Kumar T Thulasidasan

    Awarded PhD in 2015. Worked on  nanoparticle-based drug delivery and drug release for chemotherapy and chemoprevention

    jayesh
    jayesh

    Dr. Arun Kumar T Thulasidasan

    Awarded PhD in 2015. Worked on  nanoparticle-based drug delivery and drug release for chemotherapy and chemoprevention