Abitha Murali, Post Doctoral Fellow (KSCSTE)
NANOPARTICLE MEDIATED GENE SILENCING OF E6 GENES AND DOWNSTREAM SIGNALING IN HPV16 POSITIVE CERVICAL CARCINOMAS
Cervical cancer remains the most common fatal cancer in Indian women. Although surgery and chemoradiotherapy can cure 80%–95% of women with early stage cancer, the recurrent and metastatic disease remains a major cause of cancer death. Treatment strategies employing gene therapies are now being explored for treating cervical cancer. The therapeutic potential of siRNA-based therapeutics in cancer treatment has also been increasingly recognized, as numerous studies have shown the growth and proliferation of cancer cells can be greatly inhibited using this approach. Although small interfering RNAs (siRNAs) hold promise as nucleic acid-based therapeutics, effective and well-controlled in vivo delivery remains challenging as crossing biological barriers is difficult. Lipid nanoparticles have excellent biocompatibility, minimal toxicity, and are less costly comparing to polymeric carriers. In the present study, lipid nanoparticles harboring siRNA targeting HPV16 will be utilized to investigate downstream signaling and antitumor response both in in vitro and in vivo model.