Services

The Core will provide to both internal and external users at a service cost, high-sensitivity and high-resolution mass spectrometry analysis for protein and peptide characterization as its primary technology. The services offered will be:

• Basic mass analysis of peptides and proteins: Using MALDI-TOF/TOF and/or Waters LC/MS/MS (Q-TOF)
• Protein identification with MSMS sequencing: Analysis could be done by using MALDI-TOF/TOF and/or Waters LC/MS/MS (Q-TOF). Protein identifications are made using state-of-the-art database search engines running on dedicated servers, with the capability of searching standard or custom protein databases.
• Post translational modification analysis: The ETD capability of Waters Synapt G2 HDMS is an extremely powerful tool for identifying and characterising post-translational modifications, as well as the precise site within a protein where the modification occurs.
• Protein relative quantitation: Protein quantitation can be accomplished using a 'gel free, label-free' technology which provides both relative quantitation (test vs. control). Alternatively, isotope-coded (labeled) samples can be analyzed to provide relative quantitation information. The analysis will be done on Waters LC/MS/MS system.
• Protein-Protein Interaction Analysis: Protein interaction analysis will be performed by using BioRad ProteonXPR system and Waters NanoUPLC coupled HDX manager.