Rajiv Gandhi Centre for Biotechnology (RGCB), Department of Biotechnology, Government of India

General Instructions:

Contact us to discuss the experimental design of your project and sample preparation in advance.
Samples without duly filled submission forms will not be accepted.
Samples should be submitted only after making payment of analysis fee as per the invoice.
18% GST Extra as applicable
At the conclusion of analysis, data given to clients include processed data files in the form of excel spreadsheets. Unless specified, the raw data is generally not sent with results. If you need the raw data, please contact the facility. The raw data would be saved at the facility for a period of one year from the date of result communication.
Please submit application form with valid email id. Submitted application form is send to the registered email id.
Only brief description of has to be mentioned in the application form. Details can be mentioned as file attachment.

Application Form: Metabolomics/ Lipidomics Sample Submission Form
Mass Spectrometry Core Facility, RGCB

Name of Project Investigator

Contact Number

Client affiliation:

Does the project have Institutional ethical clearance:

Yes NOT Applicable

Details about the project for which the samples are going to be submitted:

Title of the project:

Background and objectives (in 2 to 4 sentences):

Relevance or rationale of performing metabolomics/lipidomics (one or 2 sentences):

Expected outcome (one or 2 sentences):

Sample Details: ((samples will be accepted as extracted metabolites or lipids etc)

Organism (species):

Sample Source or starting material

Number of samples:

Sample ID(s):

If sample id numbers are more than 15, please upload sample id list as pdf file attachment .

Sample form or condition:

Storage condition Room temperature
4°C, - 20°C, - 80°C

Starting Amount of sample or Material:

Details:

Sample grouping: sample groups that you wish to compare (e.g. "WT" & "KO", "female" & "male", "tumor" & "healthy"; for time series T1, T2 or T3, etc.)

Analysis Requirement(s): Untargeted metabolomics / lipidomics
Relative Quantification

Database to search (i.e., HMDB, KEGG, Lipid Maps etc):

Analysis requirement for Untargeted Metabolomics/Lipidomics Sample submission form

For Charges (Please refer Service Charges (Excluding GST))

Analytical Service Description

Metabolite/lipid extraction from tissue/cell/biofluids

Code: 401

Individual sample profiling or PRIDE files generation

Code: 601

Data processing using Progenesis-QI / Proteome Discoverer / Compound Discoverer software

Code: 602

D. Orbitrap Eclipse Fusion Tribrid MS connected with vanquish UHPLC system (Thermo Scientific)

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules (Combined positive & negative modes in a single UPLC-MS/MS run + data analysis)

Code: 402

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules (Positive & negative modes separately + data analysis separately for each mode)

Code: 403

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules Single mode (positive or negative mode) identification + data analysis)

Code: 404

For additional Technical replicate (By default, each sample will be injected only once for Untargeted analysis)

Code: 405

Notes/comments about samples, and description of sample preparation procedure from your lab

Any other details, upload attachment (max file size 1MB)

On submitting the application form ,you are accepting and have sole responsibility for all the details that is been uploaded and entered in the portal.

Metabolomics / Lipidomics - Sample Submission Form Mass Spectrometry Core Facility, RGCB