Profile

Research

Publications

Team

Alumni

Malini Laloraya, PhD

Scientist F

+91-471-2529462

laloraya@rgcb.res.in

malini
malini

Malini Laloraya, PhD

Scientist F

+91-471-2529462

laloraya@rgcb.res.in

  • Profile

    • PhD Life Sciences – 1990 (Devi Ahilya University, Indore, India) Thesis Title – Role of free-radicals in luteal steroidogenesis and implantation.
    • MSc Life Sciences – 1986 (Devi Ahilya University, Indore, India)
    • 2012-present: Scientist F, Rajiv Gandhi Centre for Biotechnology, Trivandrum, India
    • 2004-2012: Scientist EII, Rajiv Gandhi Centre for Biotechnology, Trivandrum, India
    • 2003-2004: Senior Lecturer , School of Life Sciences, Devi Ahilya University, Indore, India
    • 2000-2002: Visiting Scientist, University of Florida, Florida
    • 1999-2003: Lecturer , School of Life Sciences, Devi Ahilya University, Indore, India
    • 1997-1999: Scientist, School of Life Sciences, Devi Ahilya University, Indore, India
    • 1996-1997: Associate, School of Life Sciences, Devi Ahilya University, Indore, India
    • 1996-1996: Visiting Scientist, University of Virginia, Virginia
    • 1994-1995: Fellow-in-Residence, The Rockefeller University, New York
    • 1992-1994: Associate, School of Life Sciences, Devi Ahilya University, Indore, India
    • 1990-1992: Lecturer, School of Life Sciences, Devi Ahilya University, Indore, India
    • Member DBT task Force Expert Goup on Reproductive Health & Biology (2013-continuing)
    • Raine Visiting Professorship (2008) by Raine Medical Research Foundation to The University of Western Australia.
    • Nominated to Editorial Advisory board of The Open Endocrinology Journal, Open Endocrinology Reviews and Open Endocrinology Letters of Bentham Science Publishers in 2007.
    • Raine Visiting Professorship (April 2006) by Raine Medical Research Foundation to The University of Western Australia.
    • CPCSEA nominee for Madhya Pradesh Region in 2003-2004
    • Reviewer for projects for DST, DBT, BRNS, INDO-US science & technology forum
    • Reviewer for United States-Israel Binational Agricultural Research and Development Fund (BARD) proposal
    • Reviewer for several journals viz., International Journal of Pharmacognosy. Free Radical Research, Indian Journal of Biochemistry and Biophysics, Fertil Steril, Curr Sci, INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY etc.
    • Labshetwar Award (2014) of Indian Society for the Study of Reproduction & Fertility.
    • 2000-2002: Visiting Fellowship under Healthy Babies Program of NIH, Bethesda, MD University of Florida to work on Diabetes
    • 1994-1996: Recipient of an International Post-Doctoral Fellowship award The Rockefeller Foundation, New York, USA
    • lst prize of Bioscience in 6th M.P.Young Scientist Congress Madhya Pradesh Council of Science & Technology
    • 1986: Qualified in Junior Research Fellowship Examination Council of Scientific & Industrial Research, New Delhi, India
    • 1986: Qualified in National Educational Testing University Grants Commission, New Delhi, India
    • Awarded Merit Scholarship for 1984-1986 by University of Indore, Devi Ahilya Vishwavidyalaya, Indore
    • Merit certificate from Secondary Board of Education, Madhya Pradesh, for meritorious performance in H.S.C. examination
    • Member, American Association for Advancement of Science, New York.
    • Member, American Society for Human Genetics.
    • Life-Member, Society of Biological Chemists, India.
    • Life-Member, Indian Society for the Study of Reproduction and Fertility.
    • Life-Member, Society for Free Radical Research, India.
    • Life-Member, Society for Biotechnologists (India).
    • Life-Member, Society for Reproductive Biology and Comparative Endocrinology.
    • Member, Society of Study of Reproduction (SSR)
    • Member, European Society for Reproductive Immunology (ESRI).
    • http://www.freeradicalscience.com/showauthor.php?surname=Laloraya&initials=M
    • PhDs awarded: 11
    • PhDs being mentored currently: 6

    Training

    • Safety Orientation course by Department of Laboratory Safety, Rockefeller University, NY, USA
    • LARC (Laboratory Animal Research Center) training at Rockefeller University for Care and Use of Laboratory Animals, NY, USA
    • 2000-2002: Visiting Scientist, University of Florida, Florida
    • 1996-1996: Visiting Scientist, University of Virginia, Virginia
    • 1994-1995: Fellow-in-Residence, The Rocekfeller University, New York
  • Research

    THEME 1 – Molecular Mechanisms involved in Embryo Development and Implantation

    One of the most vital events for the continued existence of man-kind is the ability to reproduce. The circle of life completes its full cycle when it creates a new progeny. Pregnancy is central to this process in mammals and its success is dependent on implantation. Despite giant leaps in technologies in assisted reproduction, even when healthy embryos are transplanted, our success rate is pathetically low “up to 70 % fail to dock successfully in the uterine lining”. This is largely due to our inability to understand embryo implantation. Embryo implantation is a complex event involving apposition followed by adhesion of the blastocyst to the maternal endometrium, & finally invasion of this endometrium. For a successful implantation, the three key factors – a receptive uterus, an activated blastocyst and a conducive mileu (represented by the luminal fluid) need to be in the correct state. This event is orchestrated by steroid hormones – estrogen and progesterone. My lab endeavors to understand this complex phenomenon as theme 1 by focusing our attention on understanding mechanism of steroid action, uterine receptivity involving preparations for adhesion and invasion (viz., tissue remodeling, immune tolerance, decidualization) and attainment of embryonic pluripotency and cues for differentiation.

    Nuclear Receptor Coregulators at Implantation and implications in tumorigenesis

    Embryo implantation is a complex phenomenon involving tissue remodeling, immune tolerance, adhesion, invasion, angiogenesis which finally results in a successful pregnancy establishment. All these events are dictated by a ‘nidatory’ estrogen surge on a progesterone background which determines thewindow of uterine receptivity. Estrogen mediates steroid hormone initiated cellular signaling via nuclear receptor ERa in the uterus. Although some partners of estrogen receptor are known, it is important to decipher the estrogen receptor network in order to improve the therapies available for defective estrogen signaling. Thus, it is extremely crucial to understand the mechanism of estrogen action with special emphasis on dissecting the molecular partner networks. We have identified CrkL- an oncogene as a coactivator of ERa in the uterus during embryo implantation and the ERa-CrkL association leads to increased tumorigenesis in vitro.Hence, interacting partners can dictate the strength of ERa transactivation potential. Therefore, a major goal of our project is to gain a deep understanding of ERa partners and how they modulate estrogen receptor mediated signaling

    Free radicals & membrane dynamics at embryo implantation

    Our lab has pioneered the work on a beneficial role of superoxide radical in female reproduction. We have found an estrogen sensitive spurt in NAD(P)H oxidase generated superoxide at the window of embryo implantation which is important in ’tissue remodeling specifically the membrane fluidity’ and ‘zona hatching’. The abrogation of pregnancy by superoxide quenchers represents their novel therapeutic potential. Our recent interests now focus on mechanistic aspects of superoxide action on uterus and embryo during implantation.

    Integrin signaling at embryo implantation

    Implantation represents the remarkable synchronisation between the development of the embryo and the differentiation of the endometrium. A maternal embryo dialogue mediated by hormones, cytokines, growth factors and integrins are an important key to embryo implantation. ECM and their receptors (integrins) are very crucial in embryo-uterine interaction and in assisting the uterus in attaining receptivity. Along with integrin pathways, receptor tyrosine kinase are also functional during embryo implantation and are the two key pathways that coordinate the cellular activities during the process of blastocyst implantation. We are directing our efforts to understand the functional significance of integrin pathway mediator’s during embryo implantation.

    Immune tolerance mechanisms during pregnancy

    Proper embryo implantation is a result of the increased immune tolerance towards the semi-allogenic embryo(with its paternal set of genes) by the mother’s body and failure to produce a positive immune tolerance towards the implanting embryo always results in implantation failure which leads to abortion. The semi-allogenic embryo bearing the paternal antigens within the female uterus has to escape the immune attack by the mother’s immune system for which the mother’s immune system is suppressed by a repertoire of molecules at the maternal-fetal interface. In this project we look into maternal factors and the mechanism enabling immune tolerance towards the invading embryo.

    Molecular Mechanisms of Cell Differentiation and Pluripotency

    A fully differentiated oocyte and sperm fuse to create a totipotent zygote – a marvel where two cells fuse and result in an offspring. In such a developing embryo, a small group of cells called inner cell mass (ICM) remain pluripotent and give rise to all the cell types of the fetus and some extra embryonic tissues. The ICM cells proliferate for a limited period after which they lose their pluripotency and undergo differentiation. However, in specific culture conditions, ICM cells give rise to embryonic stem (ES) cells which can be maintained indefinitely without losing their pluripotency. We use different stages of early embryonic development and ES cells to identify key molecules in pluripotency and differentiation.

    Theme 2 – Metabolic syndromes

    Polycystic Ovarian Syndrome (PCOS)

    Polycystic ovarian syndrome is a metabolic syndrome which can negatively affect female fertility. The metabolic disorder is characterised by hyperandrogenemia, oligo/anovulation and polycystic ovaries. Familial history, altered steroidogenesis and insulin signaling pathway are speculated in the aetiology. The manifestation of the disorder involves complex interaction between environment and genetic component. We are interested in looking at non-coding RNA in PCOS which will be critical for understanding the etiology and for developing diagnostic/therapeutic strategies. We will also analyze its biological functions and molecular pathways regulated by the noncoding RNA’s.

    Diabetes

    During pregnancy a major complication is the development of Type 2 diabetes (Gestational Diabetes). The progeny has a higher risk of diabetes incidence. Also aberrant insulin signaling pathway is associated with Polycystic Ovarian Syndrome leading to Type 2 diabetes. Also diabetes is associated with male & female reproductive issues. The NOD mice – an animal model for Type 1 Diabetes show a strong female prevalence in development of diabetes. In this project we are using a proteomic approach to identify key molecules important in pancreatic islet proliferation and regeneration using pancreatic islet cell generation in vitro from embryonic stem cells and animal models of diabetes.

    PhD awarded

    1. Saguna Verma (1994)
    2. Monzy Thomas (1998)
    3. Sudhir Jain (1999)
    4. Deeksha Saxena (2002)
    5. Jaya Nautiyal (2005)
    6. Rajesh Kumar Jha (2007)
    7. Shiny Titus (2007)
    8. Mr. Zyju D. P. (2011)
    9. Ms. Renjini A. P. (2012)
    10. Ms. Jasna J. Mohan (2013)
    11. Ms. Meera Krishna B (2014)

    PhD Mentorship

    1. Mr. Philip Litto Thomas (Teacher Fellow-FDP) (2007) (Ongoing)
    2. Mr. Prashant Narayan (2010) (Ongoing)
    3. Ms. Annu Joseph (2010) (Ongoing)
    4. Ms. Soumya V (2010)(Ongoing)
    5. Ms. Betcy Susan Johnson (2015) (Ongoing)
    6. Ms. Neethu Puthumadathil (2015) (Ongoing)
    1. Saguna Verma (1993)
    2. Monzy Thomas (1998)
    3. Sudhir Jain (1999)
    4. Deeksha Saxena (2002)
    5. Jaya Nautiyal (2005)
    6. Rajesh Kumar Jha (2007)
    7. Shiny Titus (2007)
  • Publications

    Peer-Reviewed

    1. Meera B Krishna 1, Annu Joseph1, Anand G. Subramaniam 1, Arundhati Gupta1, Sathy M. Pillai2, and Malini Laloraya1. Reduced Tregs in Peripheral Blood of PCOS Patients – a consequence of aberrant Il2 signaling. J Clin Endocrinol Metab. 2014 Oct 10:jc20142401. [Epub ahead of print]
    2. A. P. Renjini, Shiny Titus, Prashanth Narayan, Megha Murali, Rajesh Kumar Jha, and Malini Laloraya STAT3 and Mcl-1 unite to cause mesenchymal epithelial transition Journal of Cell Science J Cell Sci. 2014 Apr 15;127(Pt 8):1738-50. doi: 10.1242/jcs.138214. Epub 2014 Jan 30.
    3. Maurya VK, Jha RK, Kumar V, Joshi A, Chadchan S, Mohan JJ, Laloraya M. Transforming growth factor-beta 1 (TGF-B1) liberation from its latent complex during embryo implantation and its regulation by estradiol in mouse. Biol Reprod. 2013 Oct 10;89(4):84. doi: 10.1095/biolreprod.112.106542.
    4. Chandrasekhar A, Laloraya M, Kumar PG. (2011) Modulation of nicotinamide adenine dinucleotide phosphate oxidase activity through sequential posttranslational modifications of p22 phagocytic oxidase during capacitation and acrosome reaction in goat spermatozoa. J Anim Sci. 2011 Oct;89(10):2995-3007. Epub 2011 May 6.
    5. Padmanabhan RA, Nirmala L, Murali M, Laloraya M. (2011) CrkL is a Co-Activator of Estrogen Receptor {alpha} That Enhances Tumorigenic Potential in Cancer. Mol Endocrinol. 2011 Sep;25(9):1499-512. Epub 2011 Jun 23.
    6. Brahmaraju M, Bhagya KP, Titus S, Sebastian A, Devi AN, Laloraya M, Kumar PG . (2011) AIRE1A might be involved in cyclin B2 degradation in testicular lysates. Biochem Cell Biol. Aug;89(4):411-22. doi: 10.1139/O11-029.
    7. Chandrakuntal K, Shah AK, Thomas NM, Karthika V, Laloraya M, Kumar PG and Laloraya MM (2010) Blue light exposure targets NADPH oxidase to plasma membrane and nucleus in wheat coleoptiles. J Plant Growth Reg 29, 232-241.
    8. Shoeb M, Laloraya M and Kumar PG (2010) Progesterone-induced reorganization of NOX-2 components in membrane rafts is critical for sperm functioning in Capra hircus. Andrologia 2010 Dec; 42(6), 356-65.
    9. Brahmaraju M, Laloraya M and Kumar PG (2009) Immunodominant sperm antigens and their role in male infertility. In: Current Paradigm of Reproductive Immunology, 2009: 59-81. (book chapter)
    10. S. Purohit, M. Laloraya & P. G. Kumar (2008) Distribution of N- and O-linked oligosaccharides on surface of spermatozoa from normal and infertile subjects Andrologia. 2008 Feb;40(1):7-12.
    11. Mohan JJ, Jha RK, Kumar PG, Dharmarajan AM and Laloraya M (2007) Secreted Frizzle-Related Protein (sFRP4) can abrogate pregnancy – a new dimension in its biological role. J. Endocrinol. Reprod. 11(1), 41-44.
    12. Chandran U, Laloraya M and Kumar PG (2007) Identification of testis-expressed cell cycle regulating proteins with special reference to meiosis. J. Endocrinol. Reprod. 11(1), 45-48.
    13. Selot R, Kumar V, Shukla S, Chandrakuntal K, Brahmaraju M, Dandin SB, Laloraya M, Kumar PG. (2007) Identification of a soluble NADPH oxidoreductase (BmNOX) with antiviral activities in the gut juice of Bombyx mori. Biosci Biotechnol Biochem. 2007 Jan;71(1):200-5.
    14. Jha RK, Titus S, Saxena D, Kumar PG, Laloraya M. (2006) Profiling of E-cadherin, ?-catenin and Ca2+ in embryo uterine interactions at implantation. FEBS Lett. 2006 Oct 16;580(24):5653-60.
    15. Chandrakuntal K, Thomas NM, Kumar PG, Laloraya M, Laloraya MM. (2006) Fluorescence Resonance Energy Transfer Between Polyphenolic Compounds and Riboflavin Indicates a Possible Accessory Photoreceptor Function for Some Polyphenolic Compounds. Photochem Photobiol. 2006 Sep-Oct;82(5):1358-64.
    16. Laloraya M, Davoodi-Semiromi A, Kumar GP, McDuffie M, She JX. (2006) Impaired Crkl expression contributes to the defective DNA binding of Stat5b in nonobese diabetic mice. Diabetes. 2006 Mar;55(3):734-41.
    17. Shukla S, Jha RK, Laloraya M, Kumar PG.(2005) Identification of non-mitochondrial NADPH oxidase and the spatio-temporal organization of its components in mouse spermatozoa. Biochem Biophys Res Commun. 2005 Jun 3;331(2):476-83.
    18. Purohit S, Kumar PG, Laloraya M, She JX. (2005) Mapping DNA-binding domains of the auto-immune regulator (AIRE) protein. Biochem Biophys Res Commun. 2005 Feb 18;327(3):939-44.
    19. Nautiyal J, Kumar PG, Laloraya M. Mifepristone (ru486) antagonizes monocyte chemotactic
    20. protein-3 down-regulation at early mouse pregnancy revealing immunomodulatory events in ru486 induced abortion. Am J Reprod Immunol. 2004 Jul;52(1):8-18. Chandrakuntal K, Kumar PG, Laloraya M, Laloraya MM.(2004) Direct involvement of hydrogen peroxide in curvature of wheat coleoptile in blue-light treated and dark-grown coleoptiles. Biochem Biophys Res Commun. 2004 Jul 9;319(4):1190-6.
    21. Nautiyal J, Kumar PG, Laloraya M. (2004) 17ß-estradiol induces nuclear translocation of Crk-L at the window of embryo implantation. Biochem Biophys Res Commun. 2004 May 21;318(1):103-12.
    22. Brahmaraju M, Shoeb M, Laloraya M, Kumar PG. (2004) Spatio-temporal organization of Vam6P and SNAP on mouse spermatozoa and their involvement in sperm-zona pellucida interactions. Biochem Biophys Res Commun. 2004 May 21;318(1):148-55
    23. Davoodi-Semiromi A*, Laloraya M*, Kumar GP, Purohit S, Jha RK, She JX. (2004) A mutant Stat5b with weaker DNA binding affinity defines a key defective pathway in non-obese diabetic (NOD) mice. J Biol Chem. 2004 Mar 19;279(12):11553-61. *Equal contribution
    24. Purohit S, Brahmaraju M, Palta A, Shukla S, Laloraya M, Kumar PG. Impaired E-cadherin expression in human spermatozoa in a male factor infertility subset signifies E-cadherin-mediated adhesion mechanisms operative in sperm-oolemma interactions. Biochem Biophys Res Commun. 2004 Apr 9;316(3):903-9.
    25. Shoeb M, Laloraya M, Kumar PG. (2004) Formation and Dynamic Alterations of Horizontal Microdomains in sperm Membranes During Progeterone-Induced Acrosome Reaction. Biochem Biophys Res Commun. 2004 Mar 12;315(3):763-70.
    26. Kumar PG, Laloraya M, She JX. (2002) Population genetics and functions of the autoimmune regulator (AIRE) In: Endocrinology and Metabolism Clinics of North America. Eisenbarth GD, ed. Harcourt, PA, USA, 2002 Jun;31(2):321-38, vi.
    27. Chatterjee S, Laloraya M, Kumar GP. (2001) Regional Variations in Thiol Distribution Pattern and Superoxide Dismutase Activity of the Male Reproductive Tract of the Rat Modulate the Transport of Spermatozoa through the Epididymis and Vas deferens. Urol Int. 2001;66(2):100-4.
    28. Kumar PG, Laloraya M, Wang CY, Ruan QG, Davoodi-Semiromi A, Kao KJ, She JX. (2001) The Autoimmune Regulator (AIRE) Is a DNA-binding Protein J. Biol. Chem., Vol. 276, Issue 44, 41357-41364.
    29. Chatterjee S, Rahman MM, Laloraya M, Kumar GP. (2001) Sperm disposal system in spermatic granuloma: a link with superoxide radicals. Int J Androl. 2001 Oct;24(5):278-83.
    30. Jain S, Saxena D, Kumar GP, Laloraya M. (2000) NADPH dependent superoxide generation in the ovary and uterus of mice during estrus cycle and early pregnancy. Life Sci. 66, 1139-1146.
    31. Purohit SB, Saxena D, Laloraya M, Kumar G P. (2000) Altered molecular dynamics and antioxidant status in the spermatozoa in a testosterone induced oligozoospermia model. Mol Reprod. Dev. 55(3), 316-325.
    32. Saxena D, Purohit SB, Kumer GP, Laloraya M. (2000) Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation. Nitric Oxide : Biology and Chemistry 4(4), 384-391.
    33. Laloraya MM, Chandra-kuntal K, Kumar GP, Laloraya M. (1999) Active oxygen species in blue light mediated signal transduction in coleoptile tips. Biochem. Biophys. Res. Commun. 256(2), 293-298.
    34. Agnihotri S, Purohit SB, Laloraya M, Kumar GP. (1999) Regional Heterogeneity in Intracellular Distribution of Superoxide and Hydrogen Peroxide within the sperm and its relation to Sperm Development. Archives of Andrology, 43, 113-121.
    35. Jain S, Saxena D, Kumar PG, Koide SS, Laloraya M. (1999). Effect of estradiol and selected antiestrogens on pro- and anti-oxidant pathway in mammalian uterus. Contraception 60, 111-118.
    36. Purohit SB, Laloraya M, Kumar GP. (1999) Role of ions and ion channels in capacitation and acrosome reaction of spermatozoa. Asian J. Androl. 1: 95-107.
    37. Prem P, Parihar MS, Malini L, Pradeep KG. (1998) Starvation-induced hypothyroidism involves perturbations in thyroid superoxide-SOD system in pigeons. Biochem. Mol. Biol. Int. 45(1), 73-83.
    38. Purohit SB, Laloraya M, Kumar GP. (1998) Acrosome reaction inducers impose alterations in repulsive strain and hydration barrier in human sperm membranes. Biochem. Mol. Biol. Intl. 45(2), 227-235.
    39. Purohit SB, Laloraya M, Kumar PG. (1998) Bicarbonate-dependent lipid ordering and protein aggregation are part of non-genomic action of progesterone on capacitated spermatozoa. J. Androl. 19(5),608-618.
    40. Khanna-Chopra R, Dalal M, Kumar GP, Laloraya M. (1998) A genetic system involving superoxide causes F-1 necrosis in wheat (T. aestivum L.) Biochem. Biophys. Res. Commun. 248(3), 712-715.
    41. Prakash P, Kumar GP, Laloraya M, Hemnani T, Parihar MS (1998) Superoxide anion radical generation as a temperature stress response in the gills of fresh water catfish Heteropneustes fossilis: role in mucus exudationunder elevated temperature. Comp. Biochem. Physiol. C. Pharmacol. Toxicol. Endocrinol. 119(2), 211-216.
    42. Prakash P, Laloraya M, Kumar P. (1998) Influence of a melatonin implant on the free radical load in avian thyroid and its relation with thyroid hormonogenesis. Biochem. Mol. Biol. Intl. 46(6), 1249-1258.
    43. Chatterjee S, Laloraya M, Kumar PG. (1997) Free radical induced liquefaction of ejaculated human semen: A new dimension in semen biochemistry. Arch. Androl. 38 (2), 107-111.
    44. Laloraya M, Thomas M, Jain S, Jafri F, Kumar P. (1997) Activity profile of placental superoxide-superoxide dismutase system in pregnant mice and its possible relation with placental steroidogenesis. Biochem. Mol..Biol. Intl. 42(5) 983-990.
    45. Thomas M, Jain S, Kumar GP, Laloraya M. (1997) A programmed oxyradical burst causes hatching of mouse blastocysts. J. Cell Science 110, 1597-1602.
    46. Prakash PP, Kumar GP, Laloraya M, Javeri T and Parihar MS (1997) Superoxide Anion Radical production as a cadmium-mediated mechanism of toxicity in avian thyroid: an electron spin resonance study by spin trapping. Comp. Biochem. Physiol. 118C(l) 89-95.
    47. Chatterjee S, Purohit SB, Laloraya M, Kumar P. (1997) Vasectomy induced SOD inactivation in the male reproductive tract of rat : A prerequisite for spermatic granuloma formation. Urologia Internationalis. 59(1), 23-25.
    48. Chatterjee S, Laloraya M, Kumar PG. (1997) Superoxide anion radical mediated dissection of rat sperm: An in vitro study. Arch. Androl. 39 (2), 101-104.
    49. Chatterjee S, Reddy AL, Laloraya M, Kumar PG. (1997) Free radical mediated sperm load management in the vagina of rat. Arch Androl. 1997 Sep-Oct;39(2):127-33.
    50. Singh PP, Kumar P, Laloraya M. (1997) Regulation of superoxide anion radical-superroxide dismutase system in the avial thyroid by TSH with reference to thyroid hormonogenesis. Biochem. Biophys. Res. Commun. 239 (1), 212-216.
    51. Kumar GP and Laloraya M (1997) Contraception In: Oxidative stress in male infertility (Ochsendorf FR and Fuchs J, eds.), Gardez! Verlag, St. Augustin, Germany, pp. 185-196.
    52. Negishi H, Kumar GP, Laloraya M and Koide SS (1996) Neutralization of pregnancy preventing activity of cabergoline, a dopamine agonist, with progesterone. Theriogenology 48, 241-246.
    53. Laloraya M, Jain S, Thomas M, Kopergaonkar S, Pradeep Kumar G. (1996) Estrogen surge: A regulatory switch for superoxide radical generation at implantation. Biochem. Mol.Biol. Intl. 39(5), 933-940.
    54. Nivsarkar M, Kumar GP, Laloraya M. (1996) Metal binding and resultant loss of phototoxicity of alpha-terthienyl: metal detoxification versus alpha-terthienyl inactivation. Bull. Environ. Contain. Toxicol. 56, 183-189.
    55. Kumar G P, Laloraya M, Koide SS. (1996) Characterization of a uterine luminal fluid protein ULF-250 using N-terminal microsequencing and RT-PCR identifies a novel estrogen-regulated gene in the rat uterus. FEB S Letters. 3 99, 3 3 -3 6.
    56. Thomas M, Jain S, Kumar G.P. and Laloraya M. (1995) Variation in reactive sulfhydryl group in mouse uterus in early pregnancy. Acta Histochim. et cytochim. 28(3), 1995.
    57. Sinha S, Kumar GP, Laloraya M. (1994) Abnormal physical architecture of the lipophilic domains of human sperm membrane in oligospermia: a logical cause for low fertility profiles. Biochem Biophys Res Commun. 1994 Jan 14;198(1):266-73.
    58. Thomas M, Jain S, Kumar GP, Laloraya M. (1994) Transient thiol expression and thiol-disulphide cluster-receptor communications-a molecular basis for blastocyst-uterine interactions. Biochem. Biophys. Res. Commun. 198(l),150-157.
    59. Sinha S, Kumar GP, Laloraya M. (1994) The topography of thiol-distribution on sperm surface : A sensitive marker for human fertility diagnosis. Clinica Chimica Acta 225, 83-84.
    60. Nivsarkar M, Kumar GP, Laloraya M. (1994) Photophysical alterations of erythrocyte membrane by 4,5′,8-trimethyl psoralen : Membrane response towards psoralen phototherapy. Biochem. Biophys. Res.. Commun. 199(3), 1413 -142 1.
    61. Chatterjee S, Laloraya M, Kumar GP. (1994) Free Radical Bombing of Spermatozoa in Spermatic Granuloma: An Attempt to Prevent Autoimmune Switch-On. Became. Beefiest. Res. Common. 201(l), 472-477.
    62. Laloraya MM, Pradeep KG, Laloraya M. (1994) Photochemical reaction sequences of blue light activated flavins: sensory transduction through free radical messengers. Biochem. Mol. Biol. Intl. 33(3), 543-551.
    63. Jain S, Thomas M, Kumar P, Laloraya M. (1994) Appearance of homogeneous smectic multilamellar microenviromnents in biomembranes undergoing superoxide initiated lipid peroxidation: lipid dienyl radical accumulation and fluidity management in lipid bilayers. Biochem.. Mol. Biol. Intl. 33(5), 853-862.
    64. Purohit S, Kumar GP, Laloraya M, Laloraya MM. (1994) Involvement of superoxide radical in signal transduction regulating stomatal movements. Biochem. Biophys. Res. Commun. 205(l), 30-37.
    65. Nivsarkar M, Pradeep Kumar G, Laloraya M and Laloraya MM (1993) Effect of ultraviolet activated alpha terthienyl upon anal gill membranes of mosquito larvae : A spin label study. Biochem. Syst. Ecol. 21(4), 441-447.
    66. Jain S, Thomas M, Kumar GP, Laloraya M. (1993) Programmed lipid peroxidation of biomembranes generating kinked phospholipids permitting local molecular mobility : A peroxidative theory of fluidity management. Biochem. Biophys. Res. Commun. 195(2).574-580.
    67. Sinha S, Kumar P, Laloraya M. (1993) Methyl xanthines and altered biomembrane dynamics : Demonstration of protein mobility and enzyme inhibition by caffeine in sperm model system. Biochem. Mol. Biol. Intl. 31(6), 1141-1148.
    68. Chatterjee S, Laloraya M, Kumar GP. (1993) Exorbitantly enhanced protein gyration and erroneous membrane modification programs after vasectomy: A biophysical basis for low infertility revival after vasectomy. Biochem Biophys Res Commun. 1993 Dec 15;197(2):450-456.
    69. Nivsarkar M, Kumar GP, Laloraya M, Laloraya MM. (1992) Generation of superoxide anion radical by alpha-terthienyl in the anal gills of mosquito larvae Aedes aegyptii : A new aspect in alpha terthienyl phototoxicity. Arch. Insect Biochem. Physiol. 19(4) 261-270.
    70. Purohit S, Kumar P, Laloraya M, Bharti S, Laloraya MM. (1992) ABA-induced lipid melting and its reversal by umbelliferone in the plasmalemma of the guard cell protoplast : A breakthrough in plant hormone-receptor binding and hormone action. Biochem. Biophys.. Res. Common. 182(2), 652-658.
    71. Sinha S, Pradeep KG, Laloraya M, Warikoo D. (1991) Overexpression of superoxide dismutase and lack of surface thiols in spermatozoa : Inherent defects in oligospermia. Biochem. Biophys. Res. Commun. 174(2), 510-519.
    72. Nivsarkar M, Kumar GP, Laloraya M, Laloraya MM. (1991) Superoxide dismutase in the anal gills of the larvae of mosquito Aedes aegyptii : its inhibition by alpha-terthienyl. Arch. Insect Biochem. Physiol. 16(4), 249-255.
    73. Kumar PG, Laloraya M, Laloraya MM. (1991) Superoxide radical level and superoxide dismutase activity changes in maturing mammalian spermatozoa. Andrologia 23(2),171-175.
    74. Laloraya M, Kumar GP, Laloraya MM.. (1991) Changes in the superoxide anion radical and superoxide dismutase levels in the uterus of Rattus norvegicus during the estrous cycle and a possible for superoxide radical in uterine oedema and cell proliferation at proestrus. Biochem. Cell Biol. 69 (4), 313-316.
    75. Verma S, Kumar P, Laloraya M, Nivsarkar M, Singh A. (1991) Superoxide dismutase activation in thyroid and suppression in adrenal : Novel pituitary regulatory routes. FEBS Letters 282(2), 310-312.
    76. Singh A, Kumar P, Laloraya M, Verma S, Nivsarkar M. (1991) Superoxide activity regulation by spermine : A new dimension in sperrnine biochemistry and sperm development. Biochem. Biophys. Res. Commun. 177(l), 420-426.
    77. Nivsarkar M, Pradeep Kumar G, Laloraya M and Laloraya MM (1991) Superoxide dismutase inactivation by alpha-terthienyl : A novel observation in thiophene photochemistry. Pesticide Biochem. Physiol. 41(l), 53-59.
    78. Pradeep Kumar G, Sinha S, Laloraya M, Nivsarkar M. (1991) Enhanced axial symmetry at the Fe(Ill)-heme centre of peroxidase by ascorbate : A basis for the ascorbate dependent peroxidase action. Biochem. Biophys. Res. Commun. 180(2), 597-601.
    79. Laloraya M. (1990) Fluidity of Phopholipid bilayer of the endometrium at the time of implantation of the blastocyst-a spin-label study. Biochem. Biophys. Res. Commun. 167(2), 561-567.
    80. Kumar GP, Laloraya M, Laloraya MM. (1990) Powerful anti-sperm motility action of cobaltous ion and its recovery by a sulfhydryl compound. Contraception 41, 633-639.
    81. Laloraya M, Pradeep Kumar G, Nivsarkar M and Laloraya MM (1990) Localization of superoxide dismutase in the ovary and uterus of mice during pregnancy with special reference to steroidogenesis and implantation. Acta Histochem. et Cytochem. 23(3), 271-279.
    82. Verma S, Kumar PG, Laloraya M, Singh A, Nivsarkar M, Bharti S. (1990) Discovery of a novel and alternate hydrogen peroxide generation machinery in the mammalian thyroid gland that modulates iodination of tyrosine. Biochem. Biophys. Res. Commun. 169(l), 1-7.
    83. Verma S, Kumar GP, Laloraya M, Singh A. (1990) Activation of iodine into a free radical intermediate by superoxide: A physiologically significant step in the iodination of tyrosine. Biochem. Biophys. Res. Commun. 170(3), 1026-1034.
    84. Kumar GP, Tillo N, Laloraya M, Nivsarkar M, Verma S, Singh A. (1990) Superoxide anion radical induces lipid phase transitions that mimics temperature induced phase transition phenomena of membrane lipids. Biochem. Biophys. Res. Commun. 172(2), 601-607.
    85. Pradeep KG, Seerwani N, Laloraya M, Nivsarkar M, Verma S, Singh A. (1990) Superoxide dismutase as a regulatory switch in mammalian testicular steroidogenesis. Biochem. Biophys. Res. Commun. 173(l), 302-308.
    86. Kumar P, Laloraya M,, Laloraya MM. (1989) The effect of some of the polyphenolic compounds on sperm motility in vitro: a structure-activity relationship. Contraception., 39, 531-539.
    87. Laloraya M, Kumar GP, Laloraya MM. (1989) A possible role of superoxide anion radical in the process of blastocyst implantation in Mus musculus. Biochem. Biophys. Res. Commun. 161(2), 762-770.
    88. Kumar GP, Laloraya M, Laloraya MM. (1989) Coupling of a proton pump with superoxide radical-superoxide dismutase systems in mammalian spermatozoa and its association with sperm motility. Biochem. Biophys. Res. Commun. 161(2), 771-775.
    89. Laloraya M,, Kumar GP, Laloraya MM. (1989) Histochemical study of superoxide in the ovary of the rat during the oestrous cycle. J. Reprod. Fertil. 86(2), 583-587.
    90. Kumar GP, Laloraya M, Agrawal P, Laloraya MM. (1989) The involvement of surface sugars of mammalian spermatozoa in epididymal maturation and in vitro sperm-zona recognition. Andrologia. 22(2), 179-183.
    91. Laloraya M, Pradeep KG, Laloraya MM. (1988) Changes in the levels of superoxide radical and superoxide dismutase during the estrous cycle of Rattus norvegicus and induction of superoxide dismutase by activity by lutropin. Biochem. Biophys. Res. Commun. 157(l),146-153.
    1. Meera Krishna B, Sathy M. Pillai and Malini Laloraya*. Low Circulating T Regulatory Cells: indication of autoimmune etiology in PCOS. Oral and poster presentation at 12th Congress of the International Society for Immunology of Reproduction (ISIR) hosted by the American Society for Reproductive Immunology (ASRI), Boston, MA (May 28-June 1, 2013).
    2. Meera Krishna B, Sathy M. Pillai and Malini Laloraya*. An Integrative mRNA-Transcription Factor Analysis Reveals Important PCOS Hallmarks. Oral presentation at 16th World congres on Gyneacological Endocrinology (ISGE 2014), Florence , Italy (March 5-8, 2014).
    3. Annu Joseph and Malini Laloraya* Differential pancreatic protein expression profile in type 1 diabetes mouse model: Novel insights and implications. Presented at the 17th Association for Promotion of DNA Fingerprinting and other DNA Technologies (ADNAT) convention and symposium on Genomics in Personalized Medicine and Public Health 23rd – 25th February 2014 at RGCB, Thiruvananthapuram.
    4. Jasna J. Mohan, Pradeep Kumar G and Malini Laloraya* Silencing of DOCK180 is Deleterious for Establishment of a Successful Pregnancy. Poster presented at SSR 2013, 46th Annual Meeting Reproductive Health: Nano to Global 22–26 July 2013, Montréal, Québec, Canada held at Palais des congrès de Montréal (1001 Place Jean-Paul-Riopelle, Montréal, QC, Canada).
    5. Meera Krishna B, Sathy M. Pillai and Malini Laloraya. “ARE MICRORNAS THE SILENT PLAYERS IN PATHOGENESIS OF PCOS?” Presented at International Conference on “Reproductive Health with Emphasis on Strategies for Family Planning” held at New Delhi, 19th – 21st February 2012 under the auspices of Indian Society for the Study of Reproduction and Fertility (ISSRF)
    6. Jasna J. Mohan and Malini Laloraya (2010) Dock180 tranlsocates Aire into the nucleus. presented at SBCI 2010 79TH Annual Meeting of the Society of Biological Chemists, (India) at Indian Institute of Science, Bangalore, December 13-15, 2010.
    7. Ambika Padmanabhan Renjini, Nirmala Lini, and Malini Laloraya. CrkL – an oncogene- is a co-activator of estrogen receptor alpha” presented at SBCI 2010 79TH Annual Meeting of the Society of Biological Chemists, (India) at Indian Institute of Science, Bangalore, December 13-15, 2010. Ms. Renjini AP received BEST POSTER AWARD
    8. Prashanth Narayan and Malini Laloraya (2010) Pregnancy an immune privileged instance presented at SBCI 2010 79TH Annual Meeting of the Society of Biological Chemists, (India) at Indian Institute of Science, Bangalore, December 13-15, 2010
    9. Evaluation of protein kinase c -beta in the uterus during window of embryo implantation.Rajesh Kumar Jha, Pradeep Kumar G and Malini Laloraya. National Symposium onRecent Advances in Female Reproductive Health Research, 11-12 Dec. 2008, Central Drug Research Institute (CDRI) Lucknow.
    10. Rajesh Kumar Jha, Renjini AP, Jasna J Mohan, Pradeep Kumar G & Malini Laloraya (2008) Vav-2 is an integral functional component of the activated uterine NAD(P)H oxidase at embryo implantation. International Conference on “Free Radicals & Natural Products in Health” (FRNPH-2008) and ‘Seventh Annual Meeting of the Society of Free Radical Research-India’ February 14-16, 2008, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India. RECEIVED BEST POSTER AWARD IN REPRODUCTIVE BIOLOGY SECTION.
    11. Rajesh Kumar Jha, Pradeep Kumar G & Malini Laloraya (2008) NADPH Oxidase, a superoxide generating machinery during embryo implantation in the uterus.International Conference on “Free Radicals & Natural Products in Health” (FRNPH-2008) and ‘Seventh Annual Meeting of the Society of Free Radical Research-India’ February 14-16, 2008, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India.
    12. Ashish Shah, Kumar Chandrakuntal, Neena Mary Thomas, Nima Maria George, Divya Saro Varghese, Malini Laloraya, Manmohan Manohar Laloraya and G.Pradeep Kumar CHARACTERIZATION OF NADPH OXIDASE AND ITS ROLE IN PHOTOMORPHOGENESIS IN WHEAT COLEOPTILE TIP. International Conference on “Free Radicals & Natural Products in Health” (FRNPH-2008) and ‘Seventh Annual Meeting of the Society of Free Radical Research-India’ February 14-16, 2008, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India. RECEIVED BEST POSTER AWARD IN ANTIOXIDANTS & REDOX SIGNALIING SECTION.
    13. Jasna J. Mohan, Pradeep Kumar G and Malini Laloraya DOCK180, a importin like molecule – plausible route for nuclear entry of transcription factors.The 6th Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE), December 10-14, 2007, North Bengal University, Dt. Darjeeling, West Bengal, India.
    14. Malu Ravi, Jasna J. Mohan,Sivakumar K.C., Pradeep Kumar G and Malini Laloraya, New dimensions in function of Fibulin, a secretory protein at embryo implantation. The 6th Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE), December 10-14, 2007, North Bengal University, Dt. Darjeeling, West Bengal, India.
    15. Renjini AP, Sushma Padmaja, Pradeep Kumar G and Malini Laloraya CrkL – the adapter protein – is a potential interacting partner for estrogen receptor during embryo implantation. National Symposium on Comparative Endocrinology and Reproductive Physiology:insights and Challenges, Nov 26-28 ,2007, Shantiniketan, West Bengal.RECEIVED BEST POSTER AWARD
    16. Zyju DP, Pradeep G Kumar & Malini Laloraya. Murine Son of sevenless homologue mSOS1 exhibits nuclear localization during Embryo Implantation”. National Symposium on Comparative Endocrinology and Reproductive Physiology:insights and Challenges,Nov 26-28 ,2007. Shantiniketan, West Bengal.
    17. Jasna J. Mohan*1, Rajesh Kumar Jha1, Pradeep Kumar G1, Arun M. Dharmarajan2 and Malini Laloraya1Secreted Frizzle Related Protein (sFRP4) can abrogate pregnancy – a new dimension in its biological role. SILVER JUBILEE meeting of SRBCE, Society for Reproductive Biology and Comparative Endocrinology, THIRUVANANTHAPURAM, 2007, January 15-Janury 17, 2007. (Received 2nd place in Oral presentation).
    18. Divya. L1,Rajesh Kumar.Jha2, Jasna.J.Mohan2, Pradeep. Kumar.G2, Malini.Laloraya2 & Oommen.V.Oommen1. ROS Involvement in Amphibian Metamorphosis: Confirmation by Fluorescent probes. Society for Reproductive Biology and Comparative Endocrinology [SRBCE] XXV National Symposium on Reproductive Biology and Comparative Endocrinology. January 15th – Jan17th 2007.
    19. Mohammed Shoeb, Malini Laloraya and Pradeep Kumar G Estrogen receptors are associated with sperm membrane rafts.Society for Reproductive Biology and Comparative Endocrinology [SRBCE] XXV National Symposium on Reproductive Biology and Comparative Endocrinology. January 15th – Jan17th 2007
    20. Uma Chandran, Malini Laloraya & Pradeep Kumar G. Identification of Testis expressed cell cycle regulating proteins with special reference to Meiosis.Society for Reproductive Biology and Comparative Endocrinology [SRBCE] XXV National Symposium on Reproductive Biology and Comparative Endocrinology. January 15th – Jan17th 2007. RECEIVED 1ST PRIZE IN POSTER.
    21. Shoeb M, Laloraya M and Kumar PG TRPCs are recruited thorugh RAFT-dependent mechanisms in spdrmatozoa during their post-testicular development. Poster accepted at Biochemical Society Conference on Cell and Molecular Biology of TRP Channels at University of bath, UK on September 7-8, 2006.
    22. Shoeb M, Laloraya M and Kumar PG NOX-components are associated with sperm membrane RAFTS. Poster accepted at Gordon Research Conference on NOX Family NADPH Oxidases at Les Diablerets, Switzerland on October 15-20, 2006.
    23. Shoeb M, Laloraya M and Kumar PG Formation of RAFT microdomains in sperm membranes during progesterone-induced acrosome reaction. Poster presented at ISCE 2005 – National Symposium on Comparative Endocrinology and Reproductive Physiology at University of Delhi on November 17-19, 2005
    24. Titus Shiny, Brahmaraju M, Jha Rajesh Kumar, Kumar Pradeep G, Laloraya Malini “Implantin” has a N-terminal homology to EF-1 beta and is a helix-strand-helix transcription factor . National Symposium on Comparative Endocrinology and Reproductive Physiology: retrospect and prospect,pg. 46, November 17-19, 2005, Delhi
    25. Zyju DP, Titus Shiny, Brahmaraju M, Jha Rajesh Kumar, Kumar Pradeep G & Laloraya Malini * DOCK 180 harbors a bipartite NLS and exhibits nuclear localization during embryo implantation. National Symposium on Comparative Endocrinology and Reproductive Physiology: retrospect and prospect,pg. 70, November 17-19, 2005, Delhi. RECEIVED 1ST PRIZE IN POSTER.
    26. Jha Rajesh Kumar, M. Brahmaraju, Kumar Pradeep G & Laloraya Malini * NAD(P)H oxidase is the superoxide generating machinery in implanting embryos. National Symposium on Comparative Endocrinology and Reproductive Physiology: retrospect and prospect,pg. 71, November 17-19, 2005, Delhi.
    27. Rajesh Kumar Jha, Pradeep G Kumar and Malini Laloraya*Estrogen activation of NADPH oxidase could involve the single LXD harbored in p40phox. International Symposium on Steroid Hormone Receptor Superfamily & Molecular Signaling of Nov 25-27, 2004, RGCB, Trivandrum, India.
    28. Jaya Nautiyal1, Pradeep G Kumar2 and Malini Laloraya2 ESTROGEN cascades SIGNAL TRASDUCTION EVENTS AT THE WINDOW OF EMBRYO IMPLANTATION via a plausible non-genomic pathway for estrogen action involving CrkL binding to estrogen receptor via its single LXD . International Symposium on Steroid Hormone Receptor Superfamily & Molecular Signaling of Nov 25-27, 2004, RGCB, Trivandrum, India
    29. Shiny Titus, Pradeep G Kumar and Malini Laloraya* Estrogen induces nuclear shuttling of the Auto-immune regulator “Aire” at the “window of Implantation”. International Symposium on Steroid Hormone Receptor Superfamily & Molecular Signaling of Nov 25-27, 2004, RGCB, Trivandrum, India.
    30. Kumar Chandra-Kuntal*, Manmohan M. Laloraya, G. Pradeep Kumar and Malini Laloraya. Active oxygen species in blue light mediated signal transduction in coleoptile tips Oral Presentation.In : The Blue Light Syndrome IV. International Meeting on UV/Blue Light : Perception and responses in Plants & Micro-Organisms Joint Meeting with the Research Meeting of National Institute for Basic Biology December 16-18, 2002. Okazaki Conference Center. National Institute for Basic Biology, Okazaki National Research Institute, Okazaki, JAPAN.
    31. Jaya Nautiyal1, Pradeep G Kumar2 and Malini Laloraya2 Monocyte Chemotactic Protein 3 dowregulations in early mouse pregnancy and Mifeprestone (RU486) induced upsurge- a plausible pathway for RU496 induced abortion. 23rd Meeting of the American Society of Reproductive Immunology at Yale University, New Haven, CT, USA, 18th-21st June, 2003.
    32. Jaya Nautiyal, Pradeep Kumar G & Malini Laloraya Nuclear Translocation of Stat5b at the Window of Implantation in the Uterus and Embryo in Mice Under the Influence of Estrogen. Abstract selected for a Poster presentation at the 34th Annual Meeting of the German Society of Immunology, Berlin, Germany, September 24th -27th, 2003.
    33. Davoodi-Semiromi A, Laloraya Malini, Pradeep Kumat G, Jin Xiong She. Identification and characterization of a novel mutation in DNA binding domain in Stat5b in NOD. 51st Annual Meeting of The American Society of Human Genetics, San Diego, CA, USA, October 19-23, 2001. Published in The American Journal of Human Genetics Vol 69(4) October 2001, Supplement to Volume 69.
    34. Sudhir Jain, Pradeep Kumar G., Malini Laloraya (1997). Uterine NOS activity & free radical generation during pregnancy. Oral presentation at ‘Young Scientist Symposium on Reproductive Health Issues. Organized by ICMR at Guwahati. May 1997.
    35. Sudhir Jain, , Pradeep Kumar G., Malini Laloraya (1996). Poster presentation in 10th International Congress of Endocrinology, San Fransisco, CA, USA. June 10-15.
    36. Sudhir Jain, Pradeep Kumar G., Malini Laloraya (1995). Antiestrogen modulated uterine free radical scenario and membrane dynamics. Oral presentation in 8th national conference of USI & International Symposium on Human Stones, Free Radicals & Trace metals. RNT medical college, Udaipur, India. Sep.14-16.
    37. Sudhir Jain, , Pradeep Kumar G., Malini Laloraya (1995). Poster presentation in International Symposia on prospect of ZP glycoproteins for immunocontraception & 7th annual conference of ISSRF, NII, New Delhi, Dec.2-5.
    38. Sudhir Jain, Monzy Thomas, Pradeep Kumar G., Malini Laloraya (1994). Estrogen : a regulatory switch for oxygen radical generation at implantation . Oral presentation at National Symposium on progress in hormonal research : plants and animals. June 20-22.
    39. Monzy Thomas, Sudhir Jain, , Pradeep Kumar G., Malini Laloraya (1994). Does superoxide radical initiates parturition. Selected for presentation at 16th Int. Cong.of Biochem. & Mol. Biol., Delhi, Sep. 19-22.
    40. Monzy Thomas, Sudhir Jain, , Pradeep Kumar G., Malini Laloraya (1994). Superoxide radical : a novel zona lysis factor in mouse blastocyst hatching. Selected for poster presentation at National Symposium on progress in hormonal research : plants and animals. June 20-22.
    41. Verma S.,Kumar G.P, Laloraya M (1994); Superoxide radical as a growth modulator in thyroid. 17th Annual conference of American Society for Biochem. and Mol. Biol., Delhi.
    42. Verma S., Kumar G.P, Laloraya M, Singh A, Nivsarkar M and Bharti S. (1991) A possible role of superoxide radical and SOD in enzyme iodination. 31st Annual meeting of American Society of cell Biology at Boston, USA.
    43. Laloraya M, Kumar GP & Laloraya MM. A possible role of Superoxide Anion Radical in Blastocyst Implantation at International Symposium on Biological Oxidation Systems, Bangalore, October 23-26, 1989.
    44. Kumar GP, Laloraya M, & Laloraya MM. Superoxide Anion Radical and Superoxide Dismutase in Sperm Maturation at International Symposium on Biological Oxidation Systems, Bangalore, October 23-26, 1989.
    45. Kumar PG, Laloraya M, Agrawal P and Laloraya MM Characterization of spermatozoa surfaces using lectin probes Poster accepted at Endocrine Society Conference, Kyoto, Japan, 1988.
    46. Kumar PG, Laloraya M and Laloraya MM Distribution of N-acetyl-D-glucosamine in mammalian spermatozoa in epididymal maturation and in Vitro sperm-zona attachment Poster accepted at Society of General and Comparative Endocrinology meeting at Delhi University, 1988.
    47. Laloraya M, Kumar PG, and Laloraya MM Changes in superoxide & SOD during luteal steroidogenesis, Oral Presentation. Society of General and Comparative Endocrinology meeting at Delhi University, 1988.

    Gene Bank

    1. AUTHORS Kumar G,P., Laloraya,M. and Koide,S.S.
      TITLE Characterization of a uterine luminal fluid protein ULF-250 using N-terminal microsequencing and RT-PCR identifies a novel estrogen-regulated gene in the rat uterus.
      DEFINITION Rattus norvegicus 250 kDa estrous-specific protein mRNA, partial cds.
      ACCESSION # U53183 gi|1279987|gb|U53183.1|RNU53183[1279987]

    2. AUTHORS Laloraya,M., Kumar,G.P. and Koide,S.S.
      TITLE Mouse mRNA for transformation associated protein p53 in uterus in early pregnancy .
      DEFINITION Mus musculus p53-variant (p53) mRNA, partial cds.
      ACCESSION U59758 gi|1399879|gb|U59758.1|MMU59758[1399879]

    3. AUTHORS Laloraya,M., Kumar,G.P. and Koide,S.S.
      TITLE p53 mRNA from peri-implantation blastocysts of mouse.
      DEFINITION Mus musculus p53 cellular tumor antigen (p53) mRNA, partial cds.
      ACCESSION # U59757 gi|1399877|gb|U59757.1|MMU59757[1399877]

    4. AUTHORS Laloraya,M., Kumar,G.P. and Koide,S.S.
      DEFINITION Mus musculus implantin mRNA, partial cds.
      ACCESSION U52869 gi|1256825|gb|U52869.1|MMU52869[1256825]

    5. AUTHORS Kumar,G.P. and Laloraya,M.
      TITLE Human mitochondrial gene for tRNAs
      DEFINITION Homo sapiens tRNA-Phe gene, mitochondrial gene for mitochondrial RNA, partial sequence.
      ACCESSION AF058292 gi|3047403|gb|AF058292.1|AF058292[3047403]

    6. AUTHORS Davoodi-Semiromi,A., Laloraya,M., Kumar,P. and She,J.-X.
      TITLE Mutation detection in murine STAT gene family: identification and characterization of a novel point mutation in DNA binding domain of Stat5b in NOD
      DEFINITION Mus musculus strain C3H signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds
      Accession # AY044903 gi|21654906|gb|AY044903.1|[21654906]

    7. AUTHORS Davoodi-Semiromi,A., Laloraya,M., Kumar,P. and She,J.-X.
      TITLE Mutation detection in murine STAT gene family: identification and characterization of a novel point mutation in DNA binding domain of Stat5b in NOD.
      Definition : Mus musculus strain CBA signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds.
      ACCESSION #AY044902 gi|21654904|gb|AY044902.1|[21654904]

    8. AUTHORS Davoodi-Semiromi,A., Laloraya,M., Kumar,P. and She,J.-X.
      TITLE Mutation detection in murine STAT gene family: identification and characterization of a novel point mutation in DNA binding domain of Stat5b in NOD
      DEFINITION Mus musculus strain BALB/c signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds.
      ACCESSION # AY044901 gi|21654902|gb|AY044901.1|[21654902]

    9. AUTHORS Davoodi-Semiromi,A., Laloraya,M., Kumar,P. and She,J.-X.
      TITLE Mutation detection in murine STAT gene family: identification and characterization of a novel point mutation in DNA binding domain of Stat5b in NOD
      DEFINITION Mus musculus signal transducer and activator of transcription 5b (Stat5b) mRNA, complete cds.
      ACCESSION AY042906 gi|21654874|gb|AY042906.1|[21654874]

    10. AUTHORS Davoodi-Semiromi,A., Laloraya,M., Kumar,P.G. and She,J.-X.
      TITLE Mutation detection in murine STAT gene family: identification and characterization of a novel point mutation in DNA binding domain of Stat5b in NOD
      DEFINITION Mus musculus signal transducer and activator of transcription 5B (Stat5b) mRNA, complete cds.
      ACCESSION # AY040231 gi|21654824|gb|AY040231.1|[21654824]

    11. AUTHORS Chandran,U., Laloraya,M. and Kumar,P.G.
      LOCUS DQ885890 1835 bp mRNA linear ROD 01-DEC-2006
      TITLE Cyclin-like protein 1 (CLP-1) in mouse testis
      DEFINITION Mus musculus strain Swiss cyclin-like protein 1 mRNA, complete cds.
      ACCESSION DQ885890

    12. AUTHORS Indu,S., Laloraya,M. and Kumar,P.
      TITLE Expression of Tctex-1 in mouse germ cells
      DEFINITION Mus musculus tctex-1 mRNA, complete cds.
      ACCESSION EF110999

    13. AUTHORS Selot,R., Kumar,V., Shukla,S., Chandrakuntal,K.,Brahmaraju,M., Dandin,S.B., Laloraya,M. and Kumar,P.G.
      TITLE Identification of a soluble NADPH oxidoreductase (BmNOX) with antiviral activities in the gut juice of Bombyx mori
      DEFINITION Bombyx mori NADPH oxidoreductase mRNA, partial cds.
      ACCESSION EF025315

    14. AUTHORS Brahmaraju,M., Sebastian,A., Laloraya,M. and Kumar,P.G.
      DEFINITION Mus musculus strain Swiss Albino autoimmune regulator AIRE1a mRNA, partial cds.
      ACCESSION EU625343

    15. AUTHORS Ravi,M., Kumar,P.G. and Laloraya,M.
      TITLE Expression of fibulin 1D during embryo implantation in the uterus.
      DEFINITION Mus musculus fibulin 1D variant (Fbln1) mRNA, complete cds.
      ACCESSION EU543224

    16. AUTHORS Brahmaraju,M., Thottacherry,J.J., Laloraya,M. and Kumar,P.G.
      TITLE Expression of nephrocystin in mouse testis
      DEFINITION Mus musculus nephrocystin 1 variant 1 mRNA, complete cds, alternatively spliced.
      ACCESSION EF174553

    17. AUTHORS Brahmaraju,M., Thottacherry,J.J., Laloraya,M. and Kumar,P.G.
      TITLE Expression of a testicular nephrocystin variant 2
      DEFINITION Mus musculus nephrocystin 1 variant 2 mRNA, complete cds, alternatively spliced.
      ACCESSION EF174554

    18. AUTHORS Renjini,A.P., Kumar,P.G. and Laloraya,M. July 2008
      TITLE Expression of an estrogen receptor alpha variant with an alternate 5′ UTR (U1) in mouse uterus.
      DEFINITION Mus musculus estrogen receptor alpha variant U1 mRNA, complete cds, alternatively spliced.
      ACCESSION EU791540

    19. AUTHORS Renjini,A.P., Kumar,P.G. and Laloraya,M. July 2008
      TITLE Expression of an estrogen receptor alpha variant with an alternate 5′ UTR (U2) in mouse uterus.
      DEFINITION Mus musculus estrogen receptor alpha variant U2 mRNA, complete cds, alternatively spliced.
      ACCESSION EU791538

    20. AUTHORS Jha,R.K., Narayan,P., Kumar,P.G. and Laloraya,M. July 2008
      TITLE Expression of p22phox in uterus during window of implantation
      DEFINITION Mus musculus p22phox mRNA, complete cds.
      ACCESSION EU791539

    21. AUTHORS Narayan,P., Jha,R.K., Kumar,P.G. and Laloraya,M. Sept. 2008
      TITLE Expression of p40phox in uterus during window of embryo implantation
      DEFINITION Mus musculus p40phox (Ncf4) mRNA, complete cds.
      ACCESSION FJ168470

    22. AUTHORS Narayan,P., Jha,R.K., Kumar,P.G. and Laloraya,M. Sept. 2008
      TITLE Expression of NOX2 in uterus during window of embryo implantation.
      DEFINITION Mus musculus Nox2 (Cybb) mRNA, complete cds.
      ACCESSION FJ168469

    23. AUTHORS Renjini,A.P., Soumya,V. and Laloraya,M. 04-MAY-2011
      TITLE AIRE expression during embryo implantation in mouse uteri
      DEFINITION Mus musculus strain Swiss Albino AIRE mRNA, complete cds.
      ACCESSION JF267399

    Protein Sequences in UniProt/Swiss-Prot entry
    Direct N-terminal sequenced protein:

    1. P84517 Putative NADH-ubiquinone oxidoreductase chain 4-like protein Kumar,V., Selot,R., Prasad,G., Dandn,S.B., Laloraya,M. and Kumar,P.G
      gi|62900716|sp|P84517|NU4ML_BOMMO[62900716] May 1, 2005.
    2. P83853 Vam6-like protein Brahmaraju,M., Shoeb,M., Laloraya,M. and Kumar,G.P.
      gi|46396754|sp|P83853|VAM6L_MOUSE[46396754] Submitted (??-MAR-2004) to Swiss-Prot.
    3. P83854 Putative G-protein coupled receptor Laloraya,M. and Kumar,G.P.
      gi|46395688|sp|P83854|GPCR_MOUSE[46395688] Submitted (??-MAR-2004) to Swiss-Prot
    4. P83891 Implantin Laloraya M., Kumar P.G., Koide S.S.. “A progesterone regulated transiently expressed 32.6 kDa protein ‘implantin’ at the ‘window of implantation’ has N-terminal homology to EF-1-beta.”
      gi|50400835|sp|P83891|IMPL_MOUSE[50400835] Release 45, 25-OCT-2004
    5. P83855 Putative sperm adenylate cyclase Kumar,G.P. and Laloraya,M. Submitted (??-MAR-2004) to Swiss-Prot gi|46395689|sp|P83855|ACLP_MOUSE[46395689] Submitted (??-MAR-2004) to Swiss-Prot
    6. AAC13319 estrous-specific protein [Rattus norvegicus] Kumar,G.P., Laloraya,M. and Koide,S.S. Characterization of a uterine luminal fluid protein ULF-250 usingN-terminal microsequencing and RT-PCR identifies a novel estrogen-regulated gene in the rat uterus
      gi|1279988|gb|AAC13319.1|[1279988] Submitted (30-MAR-1996).
    1. Dr. Malini Laloraya. An oxidative Approach to the Understanding of blastocyst Implantation. National Symposia on Progress in Hormonal research : Plants & Animals. Organized by School of Life Sciences, Devi Ahilya University, Indore in 1994
    2. NAD(P)H oxidase Assembly at Embryo Implantation by Estradiol – a plausible non-genomic action. Dr. Malini Laloraya. International Symposium on Steroid Hormone Receptor Superfamily & Molecular Signaling (SHRMS) held at Rajiv Gandhi Centre for Biotechnology, Trivandrum, India. 25-27 November, 2004,
    3. Molecular Signatories of Embryo Implantation. Laloraya Malini National Symposium on Comparative Endocrinology and Reproductive Physiology: retrospect and prospect,pg. 14,November 17-19, 2005, DeLHI Invited Lecture.
    4. Making sense out of antisense technology in embryo implantation research.Dr. Malini Laloraya. RGCB. TVPM.Society for Reproductive Biology and Comparative Endocrinology [SRBCE] XXV National Symposium on Reproductive Biology and Comparative Endocrinology. January 15th – Jan17th 2007.
    5. Estrogen Receptor a exhibits distinct Interactome formation in diverse sub-cellular compartments – a mechanism for it’s wide array of functions. Dr. Malini Laloraya RGCB. TVPM, The 6th Congress of the Asia and Oceania Society for Comparative Endocrinology (AOSCE), December 10-14, 2007, North Bengal University, Darjeeling, West Bengal, India.
    6. Is Embryo Implantation a superoxide mediated affair? Malini Laloraya. Society for Free Radical Research – India (SFRR) – India) Satellite Meeting. Theme :Free Radicals and Antioxidants in Human Health, Gene Regulation and Signal Transduction. February 11-12, 2008, All India Institute of Medical Sciences, New Delhi.
    7. Free Radicals and Antioxidants in Reproduction – where to draw the line. Malini Laloraya, International Conference on “Free Radicals & Natural Products in Health” (FRNPH-2008) and ‘Seventh Annual Meeting of the Society of Free Radical Research-India’ February 14-16, 2008, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India
    8. Distinguishing molecular signatures during successful embryo implantation. Malini Laloraya.National Symposium onRecent Advances in Female Reproductive Health Research, 11-12 Dec. 2008, Central Drug Research Institute (CDRI) Lucknow.
    9. Bioinformatics tools in Modern Biology. Malini Laloraya.UGC-KSCSTE Sponsored National Seminar on Bioinformatics Jan 16-17, 2009, & P K Kunj Sahib Memorial Seminar Series,M S M College,Kayamkulam
    10. Dr. Malini Laloraya. DOCK180 is important for embryo implantation : functional analysis via in vivo gene silencing experiments. Lecture at International Conference on “Reproductive Health with Emphasis on Strategies for Family Planning” held at New Delhi, 19th – 21st February 2012 under the auspices of Indian Society for the Study of Reproduction and Fertility (ISSRF).
    11. Malini Laloraya*. Intersection of Proteomics and Bioinformatics in deciphering novel functions of proteins. Invited lecture at “3rd International Conference on Proteomics & Bioinformatics” held on July 15-17, 2013 by the OMICS Group at Courtyard by Marriott Philadelphia Downtown, USA.
  • Team


    Dr. Renjini A. P, Research Associate - Level III(DST)

    Deciphering the role of ER alpha in modulating the strength of STAT3 function.

    Steroid signaling during window of implantation becomes indispensible for defining unique molecular signatures and protein networks. The uterus and the blastocyst perform dynamic homework to achieve implantation. The steroid hormone estrogen is crucial for the process of implantation and epithelial proliferation as it specifies the duration of the window of uterine receptivity for implantation. ERa, the mediator of estrogen action, is the predominant form in the murine uterus. Signal transducer and activator of transcription 3 (STAT3) is a key transcription factor during implantation acting as mediator of various cytokines. ERa interaction with STAT3 has been well documented in cancer. In this project we would try to see whether STAT3 associates with ERa, identify if the LXXLL Motif in STAT3 is responsible for this interaction and decipher the functional significance of ER-STAT3 interaction during embryo implantation.

    Renjini A. P
    Renjini A. P

    Dr. Renjini A. P, Research Associate – Level III(DST)

    Deciphering the role of ER alpha in modulating the strength of STAT3 function.

    Steroid signaling during window of implantation becomes indispensible for defining unique molecular signatures and protein networks. The uterus and the blastocyst perform dynamic homework to achieve implantation. The steroid hormone estrogen is crucial for the process of implantation and epithelial proliferation as it specifies the duration of the window of uterine receptivity for implantation. ERa, the mediator of estrogen action, is the predominant form in the murine uterus. Signal transducer and activator of transcription 3 (STAT3) is a key transcription factor during implantation acting as mediator of various cytokines. ERa interaction with STAT3 has been well documented in cancer. In this project we would try to see whether STAT3 associates with ERa, identify if the LXXLL Motif in STAT3 is responsible for this interaction and decipher the functional significance of ER-STAT3 interaction during embryo implantation.

    Dr. Sushma Padmaja, Research associate (DBT)

    Role of SFRP4 (Secreted Frizzled-Related Protein 4) in mice uterine during Early Pregnancy

    Very few developmental pathways are orchestrated during embryonic development and adult homeostasis. Some of these signal transduction pathways are intuitively involved in early pregnancy. Wnt signaling is known to be one of the decisive pathways involved in uterine growth and implantation. SFRP4 (Secreted Frizzled-Related Protein 4) is an antagonist of Wnt pathway and is known to be involved in decidualization during pregnancy. Important cellular decisions depend on the precise expression pattern and spatial distribution of signalling molecules. My study pertains to the understanding of the expression pattern and function of SFRP4 during early pregnancy.

    Sushma Padmaja
    Sushma Padmaja

    Dr. Sushma Padmaja, Research associate (DBT)

    Role of SFRP4 (Secreted Frizzled-Related Protein 4) in mice uterine during Early Pregnancy

    Very few developmental pathways are orchestrated during embryonic development and adult homeostasis. Some of these signal transduction pathways are intuitively involved in early pregnancy. Wnt signaling is known to be one of the decisive pathways involved in uterine growth and implantation. SFRP4 (Secreted Frizzled-Related Protein 4) is an antagonist of Wnt pathway and is known to be involved in decidualization during pregnancy. Important cellular decisions depend on the precise expression pattern and spatial distribution of signalling molecules. My study pertains to the understanding of the expression pattern and function of SFRP4 during early pregnancy.

    Mr. Philip Litto Thomas, Ph.D. Student (Teacher Fellow) under FACULTY DEVELOPMENT PROGRAMME, S. B. College, Changanassery

    Embryo development & acquisition of stem cell characteristics is key to its pluripotent nature, regeneration potential of embryos leading to the formation of a adult. Pluripotent nature of embryonic stem cells is characterized by the presence of certain proteins distinguished as stem cell markers. We look into the defining possible factors which can act as additional stem cell markers. We also would delve into exploring whether these factors are critical for maintaining the stem-cellness. Elucidation of pathways that lead to differentiation of stem cells into committed cell types can make significant contribution to regenerative medicines.

    PhilipLitto
    PhilipLitto

    Mr. Philip Litto Thomas, Ph.D. Student (Teacher Fellow) under FACULTY DEVELOPMENT PROGRAMME, S. B. College, Changanassery

    Embryo development & acquisition of stem cell characteristics is key to its pluripotent nature, regeneration potential of embryos leading to the formation of a adult. Pluripotent nature of embryonic stem cells is characterized by the presence of certain proteins distinguished as stem cell markers. We look into the defining possible factors which can act as additional stem cell markers. We also would delve into exploring whether these factors are critical for maintaining the stem-cellness. Elucidation of pathways that lead to differentiation of stem cells into committed cell types can make significant contribution to regenerative medicines.

    Prashanth Narayan, PhD student, ICMR

    In a gravid uterus there occurs a tight regulation of the immune system at the maternal and fetal interface so that the semi-allogenic embryo can survive. Embryo is semi-allogenic as it bears certain gene products as a result of parental gene expression and this represents a strong antigenic insult which would lead to immunological response. During the process of pregnancy it has been observed that there is increased infiltration of CD25+CD4+FOXP3+T-reg cells within the gravid uterus, these T-reg (FOXP3+) cells are known to suppress the activities of T-effector cells and bring in the effect commonly known as immune-tolerance. My work centers on understanding the mechanism of immune tolerance during embryo implantation and its regulation by steroid hormones.

    Prashanth Narayan
    Prashanth Narayan

    Prashanth Narayan, PhD student, ICMR

    In a gravid uterus there occurs a tight regulation of the immune system at the maternal and fetal interface so that the semi-allogenic embryo can survive. Embryo is semi-allogenic as it bears certain gene products as a result of parental gene expression and this represents a strong antigenic insult which would lead to immunological response. During the process of pregnancy it has been observed that there is increased infiltration of CD25+CD4+FOXP3+T-reg cells within the gravid uterus, these T-reg (FOXP3+) cells are known to suppress the activities of T-effector cells and bring in the effect commonly known as immune-tolerance. My work centers on understanding the mechanism of immune tolerance during embryo implantation and its regulation by steroid hormones.

    Annu Joseph, CSIR-SRF

    Analysis of signalling pathways involved in proliferation & regeneration of pancreatic islets

    Diabetes Mellitus is a heterogenous group of disorders, all characterized by increased plasma glucose. Type 1 diabetes, manifested mainly in children and young adults (hence called juvenile diabetes), is characterized by autoimmune destruction of insulin-producing pancreatic beta cells, leading to absolute insulin deficiency. A method of treating Type 1 diabetes is by transplantation of the Islets of Langerhans, but its application is limited due to lack of adequate supply of islets from a suitable donor, as well as constant need for immunosuppression in the patient. Regeneration of beta cells endogenously can be a solution for the alleviation of T1D. My work involves identification and analysis of molecules in various signalling pathways which can improve the proliferation, as well regeneration, of endogenous pancreas using both in vitro and in vivo models.

    Annu Joseph
    Annu Joseph

    Annu Joseph, CSIR-SRF

    Analysis of signalling pathways involved in proliferation & regeneration of pancreatic islets

    Diabetes Mellitus is a heterogenous group of disorders, all characterized by increased plasma glucose. Type 1 diabetes, manifested mainly in children and young adults (hence called juvenile diabetes), is characterized by autoimmune destruction of insulin-producing pancreatic beta cells, leading to absolute insulin deficiency. A method of treating Type 1 diabetes is by transplantation of the Islets of Langerhans, but its application is limited due to lack of adequate supply of islets from a suitable donor, as well as constant need for immunosuppression in the patient. Regeneration of beta cells endogenously can be a solution for the alleviation of T1D. My work involves identification and analysis of molecules in various signalling pathways which can improve the proliferation, as well regeneration, of endogenous pancreas using both in vitro and in vivo models.

    Soumya V, PhD Student, UGC

    Immunological adaptation of the uterus to tolerate the genetically foreign foetus is crucial in bringing successful pregnancy. Several immunologically relevant molecules have been reported to be expressed in the uterus during embryo implantation. The Autoimmune regulator (AIRE) is a transcription factor which is expressed mainly in thymus, spleen, lymph nodes and bone marrow implicating a role in central and peripheral tolerance. Its presence in various other tissues such as kidney, testis, adrenal glands, liver, and ovary has led to the suggestion that AIRE might also have a function(s) outside the immune system. Interestingly though AIRE-/- mice do not differ significantly in weight, size or maturation from their AIRE+/+ and AIRE+/- littermates, about 85% of them are infertile. In this project we aim to assess the expression, cellular compartmentalization and function of AIRE during early pregnancy.

    soumya
    soumya

    Soumya V, PhD Student, UGC

    Immunological adaptation of the uterus to tolerate the genetically foreign foetus is crucial in bringing successful pregnancy. Several immunologically relevant molecules have been reported to be expressed in the uterus during embryo implantation. The Autoimmune regulator (AIRE) is a transcription factor which is expressed mainly in thymus, spleen, lymph nodes and bone marrow implicating a role in central and peripheral tolerance. Its presence in various other tissues such as kidney, testis, adrenal glands, liver, and ovary has led to the suggestion that AIRE might also have a function(s) outside the immune system. Interestingly though AIRE-/- mice do not differ significantly in weight, size or maturation from their AIRE+/+ and AIRE+/- littermates, about 85% of them are infertile. In this project we aim to assess the expression, cellular compartmentalization and function of AIRE during early pregnancy.

    Betcy Susan Johnson, PhD Student, DST-INSPIRE

    Polycystic ovary syndrome (PCOS) affects 5-10% of women of reproductive age and is thought to be a leading cause of female sub-fertility. It is a highly complex metabolic disorder with characteristic anovulation, hyperandrogenemia and polycystic ovaries and the disorder is further compounded by insulin resistance, obesity, type 2 diabetes and high cholesterol levels. Earlier work in our lab has deciphered the miRNA-mRNA network of PCOS and identified the core pathway affected in this disorder. My work will focus on indepth study of the identified core pathway, experimental strategies to regulate the affected pathway and finally assess whether blockage of the pathway in an animal model of PCOS will provide new channels of therapy for managing PCOS.

    Betcy Susan Johnson
    Betcy Susan Johnson

    Betcy Susan Johnson, PhD Student, DST-INSPIRE

    Polycystic ovary syndrome (PCOS) affects 5-10% of women of reproductive age and is thought to be a leading cause of female sub-fertility. It is a highly complex metabolic disorder with characteristic anovulation, hyperandrogenemia and polycystic ovaries and the disorder is further compounded by insulin resistance, obesity, type 2 diabetes and high cholesterol levels. Earlier work in our lab has deciphered the miRNA-mRNA network of PCOS and identified the core pathway affected in this disorder. My work will focus on indepth study of the identified core pathway, experimental strategies to regulate the affected pathway and finally assess whether blockage of the pathway in an animal model of PCOS will provide new channels of therapy for managing PCOS.

    Neethu Puthumadathil, PhD Student, UGC

    Gestational Diabetes Mellitus is one of the most common serious complications of pregnancy and is a major cause of maternal, fetal and neonatal mortality and morbidity. GDM is associated with several complications viz., risk of intrauterine fetal death, risk of fetal macrosomia, increased frequency of maternal hypertensive disorders, need for cesarean delivery, increased risk for the development of diabetes, offspring at increased risk of obesity, glucose intolerance, and diabetes in late adolescence and young adulthood thus affecting both maternal and child health. GDM prevalence and associated maladies is on the increase around the world. Women are tested for GDM by oral glucose tolerance test (OGTT) at 6-7 weeks when GDM is already established. My work shall center around understanding the molecular markers of GDM for early diagnosis and novel therapeutic intervention.

    Neethu Puthumadathil
    Neethu Puthumadathil

    Neethu Puthumadathil, PhD Student, UGC

    Gestational Diabetes Mellitus is one of the most common serious complications of pregnancy and is a major cause of maternal, fetal and neonatal mortality and morbidity. GDM is associated with several complications viz., risk of intrauterine fetal death, risk of fetal macrosomia, increased frequency of maternal hypertensive disorders, need for cesarean delivery, increased risk for the development of diabetes, offspring at increased risk of obesity, glucose intolerance, and diabetes in late adolescence and young adulthood thus affecting both maternal and child health. GDM prevalence and associated maladies is on the increase around the world. Women are tested for GDM by oral glucose tolerance test (OGTT) at 6-7 weeks when GDM is already established. My work shall center around understanding the molecular markers of GDM for early diagnosis and novel therapeutic intervention.

    Lekshmy C R, Project Fellow, BRNS

    Diabetes mellitus is caused by a decrease in the number (Type 1) or inadequate function (Type 2) of beta cells in the islets of Langerhans in pancreas. Increased insulin synthesis, secretion and beta cell proliferation is known to be augmented by prolactin and growth hormone in both rodents and humans by activating Stat5b gene. Although, Type 1 diabetic mouse (NOD) is characterized by a L327M mutation in Stat5b while Stat5b knockouts have mild glucose intolerance, there exists a lacuna as to the mechanism by which STAT5B affects pancreatic proliferation and function. My work will focus on identifying the significance of STAT5B in beta cell proliferation, survival and function.

    lekshmi CR
    lekshmi CR

    Lekshmy C R, Project Fellow, BRNS

    Diabetes mellitus is caused by a decrease in the number (Type 1) or inadequate function (Type 2) of beta cells in the islets of Langerhans in pancreas. Increased insulin synthesis, secretion and beta cell proliferation is known to be augmented by prolactin and growth hormone in both rodents and humans by activating Stat5b gene. Although, Type 1 diabetic mouse (NOD) is characterized by a L327M mutation in Stat5b while Stat5b knockouts have mild glucose intolerance, there exists a lacuna as to the mechanism by which STAT5B affects pancreatic proliferation and function. My work will focus on identifying the significance of STAT5B in beta cell proliferation, survival and function.

    Ms. Selin Joseph, Technical assistant (DST)

    I will be involved in lab management, technical assistance, ordering and overall enable smooth lab operations. I will also assist Dr. Renjini AP, Research associate in the DST project on understanding the significance of ERa-STAT3 interaction during embryo implantation.

    Ms. Selin Joseph, Technical assistant (DST)

    I will be involved in lab management, technical assistance, ordering and overall enable smooth lab operations. I will also assist Dr. Renjini AP, Research associate in the DST project on understanding the significance of ERa-STAT3 interaction during embryo implantation.

    Sheela G, Technical Assistant

    sheelag
    sheelag

    Sheela G, Technical Assistant

  • Alumni


    Post-Doctoral Fellows

    Dr. Rajesh Kumar Jha (2008-2010)

    Dr. Rajesh Jha was a DST SERC Fast Track Young Scientist in my laboratory. He worked on assessing the mechanism of Tansforming gowth factor ß1 release from its latent complex duirng embryo implantation
    http://www.ncbi.nlm.nih.gov/pubmed/23926286

    Dr. Rajesh Kumar Jha
    Dr. Rajesh Kumar Jha

    Dr. Rajesh Kumar Jha (2008-2010)

    Dr. Rajesh Jha was a DST SERC Fast Track Young Scientist in my laboratory. He worked on assessing the mechanism of Tansforming gowth factor ß1 release from its latent complex duirng embryo implantation
    http://www.ncbi.nlm.nih.gov/pubmed/23926286

    Dr. Lini Nirmala (2009-2010)

    Lini is a doctorate in Biotechnology from Madurai Kamaraj Universtity, Madurai, Tamil adu under Prof. Dharmalingam.
    She is an Assistant Professor, Mar Ivanios College, Thiruvananthapuram snce 2012.
    Prior to this she was a Scientist D in Division of Proteomics , AMRF, Madurai (2010 – 2012) where she worked on Comparative proteomic profiling of serum and vitreous fluid of patients suffering from proliferative diabetic retinopathy and inflammatory mediators of the disease to understand the mechanism of pathogenesis of disease.
    Dr. Lini was an ICMR-RA during Feb 2009-Aug 2010. She worked on the functional significance of Elk1in embryo implantation.

    Lini
    Lini

    Dr. Lini Nirmala (2009-2010)

    Lini is a doctorate in Biotechnology from Madurai Kamaraj Universtity, Madurai, Tamil adu under Prof. Dharmalingam.
    She is an Assistant Professor, Mar Ivanios College, Thiruvananthapuram snce 2012.
    Prior to this she was a Scientist D in Division of Proteomics , AMRF, Madurai (2010 – 2012) where she worked on Comparative proteomic profiling of serum and vitreous fluid of patients suffering from proliferative diabetic retinopathy and inflammatory mediators of the disease to understand the mechanism of pathogenesis of disease.
    Dr. Lini was an ICMR-RA during Feb 2009-Aug 2010. She worked on the functional significance of Elk1in embryo implantation.

    PhDs

    Dr. Saguna Verma (1989-1994)

    Saguna obtained her Ph .D. in 1993. She worked on redox biology in thyroid and adrenal steroidogenesis and identified the superoxide anion radical-mediated active I radical form – the intermediate that is incorporated onto tyrosine. Post doctoral fellow at NII, Delhi in lab of Dr. Anil Suri. Junior researcher and Assistant Professor University of Hawaii. She is Currently Associate Professor (tenure-track), Department Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii. Leads a group to study RNA virus-host interactions. Her lab uses both, in vitro and in vivo models to delineate various innate immune signaling pathways that contribute to inflammation and pathology in flavivirus infections including West Nile virus and Dengue virus.
    http://pceidr.jabsom.hawaii.edu/guest/Guest.vm?method=homePage&id=15

    Dr. Saguna Verma
    Dr. Saguna Verma

    Dr. Saguna Verma (1989-1994)

    Saguna obtained her Ph .D. in 1993. She worked on redox biology in thyroid and adrenal steroidogenesis and identified the superoxide anion radical-mediated active I radical form – the intermediate that is incorporated onto tyrosine. Post doctoral fellow at NII, Delhi in lab of Dr. Anil Suri. Junior researcher and Assistant Professor University of Hawaii. She is Currently Associate Professor (tenure-track), Department Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii. Leads a group to study RNA virus-host interactions. Her lab uses both, in vitro and in vivo models to delineate various innate immune signaling pathways that contribute to inflammation and pathology in flavivirus infections including West Nile virus and Dengue virus.
    http://pceidr.jabsom.hawaii.edu/guest/Guest.vm?method=homePage&id=15

    Dr. Monzy Thomas (1993-1997)

    Monzy was awarded his Ph. D. in 1998. His seminal work led to the discovery of superoxide mediated embryo hatching and membrane perturbations prior to embryo implantation. PDF with Prof. Polani . B. Seshagiri at MRDG, IISc 1997-2001, Post Doctoral Fellow at Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, Scientist at FDA. Currently, Scientist at Aptagen, LLC, 250 North Main Street, Jacobus, PA, USA.

    Dr. Monzy Thomas
    Dr. Monzy Thomas

    Dr. Monzy Thomas (1993-1997)

    Monzy was awarded his Ph. D. in 1998. His seminal work led to the discovery of superoxide mediated embryo hatching and membrane perturbations prior to embryo implantation. PDF with Prof. Polani . B. Seshagiri at MRDG, IISc 1997-2001, Post Doctoral Fellow at Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan, Scientist at FDA. Currently, Scientist at Aptagen, LLC, 250 North Main Street, Jacobus, PA, USA.

    Dr. Sudhir Jain, PhD (1992-1997)

    Sudhir obtained his Ph. D. in 1999. His thesis work identified superoxide mediated increased lipid fluidity due to dienyl radical formation in uterine membrane at embryo implantation. PDF at National Institute of Immunology, Delhi, India(1997-1999), Animal Science Department, Rutgers University, New Brunswick, New Jersey (Jan 2000-Oct. 2000). New York Medical College, Valhalla, New York (Oct.2000-Dec. 2003);Research Assistant Professor, New York Medical College, Valhalla, New York (Jan. 2004-Sept.2011). He is currently an Assistant Professor from 2011 at Department of Physiology and Pharmacology, University of Toledo College of Medicine,Toledo, Ohio , USA. He is working in the field of human hypertension and associated pathologies. He is studying the role of genetic variants in the transcriptional regulation of genes associated with human renin angiotensin aldosterone pathway. In this regard, he has generated various transgenic mice models harboring the genetic variants genes in RAAS. To get in touch contact, Ph. 9142555453 fax: 419 383 2871, sudhir.jain@utoledo.edu .
    https://www.utoledo.edu/med/depts/physpharm/faculty/jain.html

    Dr. Sudhir Jain
    Dr. Sudhir Jain

    Dr. Sudhir Jain, PhD (1992-1997)

    Sudhir obtained his Ph. D. in 1999. His thesis work identified superoxide mediated increased lipid fluidity due to dienyl radical formation in uterine membrane at embryo implantation. PDF at National Institute of Immunology, Delhi, India(1997-1999), Animal Science Department, Rutgers University, New Brunswick, New Jersey (Jan 2000-Oct. 2000). New York Medical College, Valhalla, New York (Oct.2000-Dec. 2003);Research Assistant Professor, New York Medical College, Valhalla, New York (Jan. 2004-Sept.2011). He is currently an Assistant Professor from 2011 at Department of Physiology and Pharmacology, University of Toledo College of Medicine,Toledo, Ohio , USA. He is working in the field of human hypertension and associated pathologies. He is studying the role of genetic variants in the transcriptional regulation of genes associated with human renin angiotensin aldosterone pathway. In this regard, he has generated various transgenic mice models harboring the genetic variants genes in RAAS. To get in touch contact, Ph. 9142555453 fax: 419 383 2871, sudhir.jain@utoledo.edu .
    https://www.utoledo.edu/med/depts/physpharm/faculty/jain.html

    Dr. Deeksha Saxena (1997-2002)

    Deeksha worked on Characterization of Implantation Specific Proteins in the mouse uterus. Post doctoral fellow at Department of Obstetrics, Gynecology & Reproductive Sciences, University of Pittsburgh. Currently PDF and Lab Manager in Csiki Lab, Perelman Center for Advanced Medicine, Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA , USA. She works on sonic hedgehog pathway in lung tumorigenesis.
    http://www.xrt.upenn.edu/CsikiLab.shtml

    Dr. Deeksha Saxena
    Dr. Deeksha Saxena

    Dr. Deeksha Saxena (1997-2002)

    Deeksha worked on Characterization of Implantation Specific Proteins in the mouse uterus. Post doctoral fellow at Department of Obstetrics, Gynecology & Reproductive Sciences, University of Pittsburgh. Currently PDF and Lab Manager in Csiki Lab, Perelman Center for Advanced Medicine, Department of Radiation Oncology, University of Pennsylvania, Philadelphia, PA , USA. She works on sonic hedgehog pathway in lung tumorigenesis.
    http://www.xrt.upenn.edu/CsikiLab.shtml

    Dr. Jaya Nautiyal (1999-2005)

    Jaya obtained her Ph. D in 2005. She identified novel molecular markers regulated during the Window of Implantation. She showed the nulear translocation of CrkL (an oncogene) in the uterus during the implantation period. She was a postdoctoral fellow at All India Institute of Medical Sciences, New Delhi (India) after which she moved to Institute of Reproductive and Developmental Biology (IRDB) at Imperial College in 2006. Currently, she is a Research Associate Faculty of Medicine, Department of Surgery & Cancer, Institute of Reproductive and Developmental Biology, Hammersmith Campus, Imperial College of London,UK. The focus of her research has been to study the role of nuclear receptor and growth factor signalling in influencing developmental processes like ovulation and mammary gland development with the goal to understand perturbed signalling in infertility and cancer.
    http://www.imperial.ac.uk/AP/faces/pages/read/Home.jsp?person=j.nautiyal&_adf.ctrl-state=cz9f9tkps_95&_afrRedirect=2626880675912529

    Dr. Jaya Nautiyal
    Dr. Jaya Nautiyal

    Dr. Jaya Nautiyal (1999-2005)

    Jaya obtained her Ph. D in 2005. She identified novel molecular markers regulated during the Window of Implantation. She showed the nulear translocation of CrkL (an oncogene) in the uterus during the implantation period. She was a postdoctoral fellow at All India Institute of Medical Sciences, New Delhi (India) after which she moved to Institute of Reproductive and Developmental Biology (IRDB) at Imperial College in 2006. Currently, she is a Research Associate Faculty of Medicine, Department of Surgery & Cancer, Institute of Reproductive and Developmental Biology, Hammersmith Campus, Imperial College of London,UK. The focus of her research has been to study the role of nuclear receptor and growth factor signalling in influencing developmental processes like ovulation and mammary gland development with the goal to understand perturbed signalling in infertility and cancer.
    http://www.imperial.ac.uk/AP/faces/pages/read/Home.jsp?person=j.nautiyal&_adf.ctrl-state=cz9f9tkps_95&_afrRedirect=2626880675912529

    Dr. Rajesh Kumar Jha (2001-2007)

    Rajesh was awarded his Ph.d. in 2007. He Characterized the NAD(P)H Oxidase Components and its Signaling Mechanism during Embryo Implantation. He was a Post Doctoral Fellow in Andrology Division, Glickman Urological Institute , Cleveland Clinic Foundation, USA and later a DST Fast Track Scientist in Division of Molecular Reproduction, RGCB, Trivandrum, India. Currently he is a Scientist & Assistant Professor in Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India. His research is focused on cellular and molecular signatories (TGF-ß, Endoglin (CD105), PKC, PARP, integrins and RhoGTPase) of female reproduction biology covering ovarian follicle development, embryo implantation/uterine receptivity, endometriosis and PCOS pathophysiology. Additionally, we are also studying the potential targetable cellular pathways for female contraceptive development.
    http://www.cdriindia.org/rdivision5.htm

    Dr. Rajesh Kumar Jha Phd
    Dr. Rajesh Kumar Jha Phd

    Dr. Rajesh Kumar Jha (2001-2007)

    Rajesh was awarded his Ph.d. in 2007. He Characterized the NAD(P)H Oxidase Components and its Signaling Mechanism during Embryo Implantation. He was a Post Doctoral Fellow in Andrology Division, Glickman Urological Institute , Cleveland Clinic Foundation, USA and later a DST Fast Track Scientist in Division of Molecular Reproduction, RGCB, Trivandrum, India. Currently he is a Scientist & Assistant Professor in Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, India. His research is focused on cellular and molecular signatories (TGF-ß, Endoglin (CD105), PKC, PARP, integrins and RhoGTPase) of female reproduction biology covering ovarian follicle development, embryo implantation/uterine receptivity, endometriosis and PCOS pathophysiology. Additionally, we are also studying the potential targetable cellular pathways for female contraceptive development.
    http://www.cdriindia.org/rdivision5.htm

    Dr. Shiny Titus (2002-2007)

    Shiny obtained her Ph. D. in 2007. She worked on Identifying Transcription Factors Involved in Embryo Activation and Implantation. She was a Post doctoral fellow at National Centre For Cell Sciences Pune, where she was involved in characterizing the factors regulating the migration and selection of T cells in different thymic compartments. She is currently a Postdoctoral Fellow, Department of Obstetrics & Gynecology, New York Medical College, Valhalla, NY. Her research focus is on elucidating the mechanisms of ovarian aging and fertility preservation.

    Dr. Shiny Titus
    Dr. Shiny Titus

    Dr. Shiny Titus (2002-2007)

    Shiny obtained her Ph. D. in 2007. She worked on Identifying Transcription Factors Involved in Embryo Activation and Implantation. She was a Post doctoral fellow at National Centre For Cell Sciences Pune, where she was involved in characterizing the factors regulating the migration and selection of T cells in different thymic compartments. She is currently a Postdoctoral Fellow, Department of Obstetrics & Gynecology, New York Medical College, Valhalla, NY. Her research focus is on elucidating the mechanisms of ovarian aging and fertility preservation.

    Dr. Zyju DP (2005-2010)

    Zyju was awarded his Ph.D in 2011. His doctoral work involved understanding SOS1 function during embryo implantation. Zyju is currently, Manager Application Support, Al Essa Medical & Scientific Equipment Company, Kuwait. He is involved in providing technical support to researchers on new technologies including Next Generation Sequencing. Their customers include Scientists of various university departments and Research institutes across Kuwait. To contact, write to: zyju.dp@gmail.com

    Dr. Zyju DP
    Dr. Zyju DP

    Dr. Zyju DP (2005-2010)

    Zyju was awarded his Ph.D in 2011. His doctoral work involved understanding SOS1 function during embryo implantation. Zyju is currently, Manager Application Support, Al Essa Medical & Scientific Equipment Company, Kuwait. He is involved in providing technical support to researchers on new technologies including Next Generation Sequencing. Their customers include Scientists of various university departments and Research institutes across Kuwait. To contact, write to: zyju.dp@gmail.com

    Dr. Renjini A P (2006-2011)

    Renjini obtained her Ph. D. in 2012. Her doctoral work lead to identification of CrkL as a partner of ERa using proteomic tools. She finally deciphered it to be a coactivator of ERa action and the interaction increases tumorigenesis. She is currently a Post doctoral Fellow in my lab and focuses on implantation specific protein interactomes and their significance.

    Dr. Renjini A.P alumni
    Dr. Renjini A.P alumni

    Dr. Renjini A P (2006-2011)

    Renjini obtained her Ph. D. in 2012. Her doctoral work lead to identification of CrkL as a partner of ERa using proteomic tools. She finally deciphered it to be a coactivator of ERa action and the interaction increases tumorigenesis. She is currently a Post doctoral Fellow in my lab and focuses on implantation specific protein interactomes and their significance.

    Dr. Jasna J Mohan (2006-2011)

    Jasna obtained her Ph. D. in 2013. She worked on Understanding the ‘modus operandi’ of DOCK mediated signaling at embryo implantation. She was a SRF in Department of Biochemistry and Cell Biology,Vision Research Foundation, Sankara Nethralaya, Chennai (July 2011- June 2013) where she worked on ‘evaluation of pesticide chloryrifos on the proteome of human retinal pigment epithelial cell line’. She is currently a Research Associate, Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, Chennai since July 2013. Her current study involves the ‘role of tumour microenvironment and epigenetic regulation in Retinoblastoma along with aptamer based therapeutics’.

    Dr. Jasna J. Mohan
    Dr. Jasna J. Mohan

    Dr. Jasna J Mohan (2006-2011)

    Jasna obtained her Ph. D. in 2013. She worked on Understanding the ‘modus operandi’ of DOCK mediated signaling at embryo implantation. She was a SRF in Department of Biochemistry and Cell Biology,Vision Research Foundation, Sankara Nethralaya, Chennai (July 2011- June 2013) where she worked on ‘evaluation of pesticide chloryrifos on the proteome of human retinal pigment epithelial cell line’. She is currently a Research Associate, Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, Chennai since July 2013. Her current study involves the ‘role of tumour microenvironment and epigenetic regulation in Retinoblastoma along with aptamer based therapeutics’.

    Dr. Meera Krishna B (2008-2014)

    Meera obtained her Ph. D. in 2015. She worked on Evaluation of molecular defects in Polycystic OvarY Syndrome. Meera is Currently working as Cell Processing Specialist at Ovascience in Dubai, UAE. Ovascience is a global fertility company dedicated to improving treatment options for women around the world. To contact, write to: meerabkrishna@gmail.com

    meera krishnan
    meera krishnan

    Dr. Meera Krishna B (2008-2014)

    Meera obtained her Ph. D. in 2015. She worked on Evaluation of molecular defects in Polycystic OvarY Syndrome. Meera is Currently working as Cell Processing Specialist at Ovascience in Dubai, UAE. Ovascience is a global fertility company dedicated to improving treatment options for women around the world. To contact, write to: meerabkrishna@gmail.com