Rajiv Gandhi Centre for Biotechnology (RGCB), Department of Biotechnology, Government of India

General Instructions:

Outside RGCB clients: please ship samples in sufficient dry ice.
Contact us to discuss the experimental design of your project and sample preparation in advance.
Samples without duly filled submission forms will not be accepted.
At the conclusion of analysis, data given to clients include processed data files in the form of excel spreadsheets. Unless specified, the raw data is generally not sent with results. If you need the raw data, please contact the facility. The raw data would be saved at the facility for a period of one year from the date of result communication.
Please submit application form with valid email id. Submitted application form is send to the registered email id.
On submitting the application form, you are accepting and have sole responsibility for all the details that is been uploaded and entered in the portal.

Application Form: Metabolomics/ Lipidomics Sample Submission Form
Mass Spectrometry Core Facility, RGCB

Name of Project Investigator

Contact Number

Client affiliation:

Details about the project for which the samples are going to be submitted:

Does the project have Institutional ethical clearance:

Yes NOT Applicable

Title of the project:

Background and objectives (in 2 to 4 sentences):

Relevance or rationale of performing metabolomics/lipidomics (one or 2 sentences):

Expected outcome (one or 2 sentences):

SAMPLE DETAILS: (Samples will be accepted as dried extracts obtained using speedvac / under nitrogen (preferred at cool conditions or room temperature) or aqueous methanolic extracts)

Number of samples:

Sample ID(s):

Sample Source or starting material

Starting Amount of sample or Material:

Storage condition Room temperature
4°C, - 20°C, - 80°C

Notes/comments about samples, and description of sample preparation procedure from your lab

Compound/metabolite/lipid details :

Number of targeted compounds per sample:

Name and molecular mass of the targeted compounds:

Polarity of the compounds (polar, semi polar, non-polar):

Standard type: External/Internal

External Internal

Availability of standard with the client:

Yes No

Storage condition of the standard (RT, 4 ° C, -20 ° C):

Class of the compounds (e.g. sugar, amino acid, fatty acid, esters, organic acids etc.):

Extraction method including solvent detail (In 4 to 6 sentences):

Final dissolving solvent :

LC Column Details:

Any specific name/brand of the column (e.g. Luna C₁₈):

Nature of the column (Reverse phase/ Normal phase):

Column dimensions:

Column temperature:

Availability of the column with the client:

Yes No

Analysis requirement for Targeted Metabolomics/Lipidomics Sample submission form

For Charges (Please refer Service Charges (Excluding GST))

E. Altis Plus Triple Quadrupole MS connected with vanquish UHPLC system (Thermo Scientific)

Metabolite/lipid extraction from tissue/ cell/ biofluids

Code: 401

ESI-MS based analysis mass/MW. Determination (without UPLC )

Code: 501 (would be done only for pure compound.)

UPLC-MS/MS for Targeted analysis of Lipids/Small molecules (Standards should be procured by the investigator)

Code: 502

UPLC-MS/MS for Targeted analysis of Lipids / Small molecules (already present in our panel)

Code: 503

Additional Technical replicate (By default, each sample will be injected only once for Targeted analysis of Lipids/Small molecules)

Code: 504

Any suggested LC-MS/MS analytical method (in 4 to 6 sentences):

Any reference papers (paste the link address or type the title in the space below)

Any other details, upload attachment (max file size 1MB)

On submitting the application form ,you are accepting and have sole responsibility for all the details that is been uploaded and entered in the portal.

Sample Submission Form Targeted Metabolomics / Lipidomics or Absolute Quantification RGCB - Mass Spectrometry Core Facility