S. No

Code

Analytical Service Description

Academia & Start-Ups Clients

Non-Academia & Overseas Clients

1

101

Protein extraction from tissues and cells

₹ 3,000 / sample

₹ 4,500 / sample

2

110

Desalting of protein lysate [compulsory for Synapt XS (Q/TOF) mass spectrometer]

₹ 2,500 / sample

₹ 3,750 / sample

3

111

Protein estimation (BCA or Bradford assay)

₹ 1,000 / sample

₹ 1,500 / sample

4

301

Peptide purification using Zip-tips or Pierce C18 Spin Columns [compulsory for Orbitrap Eclipse mass spectrometer]

₹ 2,500 / sample

₹ 3,750 / sample

5

401

Metabolite/lipid extraction from tissue/cell/biofluids

₹ 2,500 / sample

₹ 3,750 / sample

6

601

Individual sample profiling or PRIDE files generation

₹ 1,500 / hour

₹ 2,250 / hour

7

602

Data processing using Progenesis-QI / Proteome Discoverer / Compound Discoverer software

₹ 1,000 / hour

₹ 1,500 / hour

A

SYNAPT XS HDMS connected with ACQUITY UPLC M-Class System (Waters)

8

102

65 min nanoLC-MS/MS run #
(For those who require machine generated Waters .RAW files alone)

₹ 2,000 / sample

₹ 3,000 / sample

9

103

133 min nanoLC-MS/MS run #
(For those who require machine generated Waters .RAW files alone)

₹ 3,500 / sample

₹ 5,250 / sample

10

104

Protein Profiling of digested sample s#
(133 min min nanoLC-MS/MS run +Database search)

₹ 5,000 / sample

₹ 7,500 / sample

11

105

Identification of proteins from coomassie stained gel band or gel spot
(65 min nanoLC-MS/MS run + Protein digestion + Database search)

₹ 5,000 / sample

₹ 7,500 / sample

12

106

Protein Profiling for less complex protein mixtures (i.e., purified protein) (65 min nanoLC-MS/MS run + Protein digestion + Database search)

₹ 5,000 / sample

₹ 7,500 / sample

13

107

Complex proteome analysis (discovery)
(133 min nanoLC-MS/MS run + Protein digestion + Database search)

₹ 6,500 / sample

₹ 9,750 / sample

14

108

Relative protein quantification by Label-free method
(133 min nanoLC-MS/MS run + Protein digestion + Database search + Quantitation)

₹ 7,000 / sample

₹ 10,500 / sample

15

109

For additional Technical replicate (additional nanoLC-MS/MS run)
(By default, each sample will be injected only 2 times)

₹ 1,500 / injection

₹ 2,250 / injection

B

UltraFlextreme MALDI-TOF/TOF (Bruker Daltonics)

16

201

Molecular weight (accurate mass) determination.

₹ 2,500 / sample

₹ 3,750 / sample

17

202

Polymer analysis

₹ 2,500 / sample

₹ 3,750 / sample

18

203

Oligo-nucleotides molecular weight determination

₹ 2,500 / sample

₹ 3,750 / sample

C

Orbitrap Eclipse Fusion Tribrid MS connected with UltiMate 3000 RSLCnano UHPLC system (Thermo Scientific)

19

302

60 min nanoLC-MS/MS run#
(For those who require machine generated Thermo .RAW files alone)

₹ 4,000 / sample

₹ 6,000 / sample

20

303

140 min nanoLC-MS/MS run#
(For those who require machine generated Thermo .RAW files alone)

₹ 6,000 / sample

₹ 9,000 / sample

21

304

Deep proteomics profiling of digested samples#
(140 min nanoLC-MS/MS run + Database search)

₹ 7,500 / sample

₹ 11,250 / sample

22

305

Identification of proteins from coomassie blue stained gels or immunoprecipitates (Includes: 60 min nanoLC-MS/MS run, protein digestion, peptide purification, and database search — qualitative data only )

₹ 9,000 / sample

₹ 13,500 / sample

23

306

Deep proteomics profiling (>2,500) from tissue/cell/biofluids.(Includes: 140 min nanoLC-MS/MS run, protein digestion, peptide purification, and database search — qualitative data only unless label-free protein quantification is additionally opted [Code: 312] )

₹ 11,000 / sample

₹ 16,500 / sample

24

307

Labeled Quantification (TMT/iTRAQ) – Post-Fractionation (Total of 5 Fractions) (145 min × 5 = 725 min nanoLC-MS/MS Runs + Database Search)
Note: Labeling and fractionation should be performed by the customer. The stated service fee applies to a single replicate comprising 5 fractions. For additional technical replicates, the fee will be charged per replicate accordingly.

₹ 16,000

₹ 24,000

25

308

Identification of phosphorylation and other post-translational modification using ETD fragmentation
(60 min nanoLC-MS/MS run + Protein digestion + Peptide purification + Database search)*

₹ 9,000 / sample

₹ 13,500 / sample

26

309

Identification of phosphorylation and other post-translational modification using ETD fragmentation (140 min nanoLC-MS/MS run + Protein digestion + Peptide purification + Database search)*

₹ 11,000 / sample

₹ 16,500 / sample

27

310

Profiling of phosphorylated proteins after phospho-enrichment using the Fe-NTA Phosphopeptide Enrichment Kit (140-minute nanoLC-MS/MS run + de-salting + protein digestion + Fe-NTA phosphopeptide enrichment + database searching)
Note: The Fe-NTA phosphopeptide enrichment will be performed by the facility and requires 0.5 mg to 5 mg of total protein digest as starting sample material.

₹ 14,000 / sample

₹ 21,000 / sample

28

311

For additional technical replicates:

For proteomics services other than labeled quantification (Code: 307), such as TMT or iTRAQ, each sample is injected twice by default.

₹ 4,000 / injection

₹ 6,000 / injection

29

312

For Deep Proteomics Profiling (Code: 306): an additional charge of ₹ 750 per comparison will be applied for label-free protein quantification analysis.

₹ 750 / pair

₹ 1,125 / pair

D

Orbitrap Eclipse Fusion Tribrid MS connected with vanquish UHPLC system (Thermo Scientific)

30

402

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules
(Combined positive & negative modes in a single UPLC-MS/MS run + data analysis)

₹ 4,000 / sample

₹ 6,000 / sample

31

403

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules
(Positive & negative modes separately + data analysis separately for each mode)

₹ 6,000 / sample

₹ 9,000 / sample

32

404

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules
Single mode (positive or negative mode) identification + data analysis)

₹ 3,000 / sample

₹ 4,500 / sample

33

405

For additional Technical replicate

(By default, each sample will be injected only once for Untargeted analysis)

₹ 2,500 / injection

₹ 3,750 / injection

E

Altis Plus Triple Quadrupole MS connected with vanquish UHPLC system (Thermo Scientific)

34

501

ESI-MS based analysis mass/MW. Determination (without UPLC )*

₹ 1,500 / sample

₹ 2,250 / sample

35

502

UPLC-MS/MS for Targeted analysis of Lipids/Small molecules (Standards should be procured by the investigator)

5,000/sample + 300 per precursor ion

7,500/sample + 450 per precursor ion

36

503

UPLC-MS/MS for Targeted analysis of Lipids / Small molecules (already present in our panel)

2,500/sample + 300 per precursor ion

3,750/sample + 450 per precursor ion

37

504

Additional Technical replicate (By default, each sample will be injected only once for Targeted analysis of Lipids/Small molecules)

₹ 1,500 / injection

₹ 2,250 / injection

Notes

18% GST Extra as applicable

* Code no.501 would be done only for pure compound.

# Code no.102, 103, 104, AND 302, 303,304would be done only for purified peptides (after doing ZipTip or spin columns).