DBT-SAHAJ National Facility for Mass Spectrometry-Based Proteomics, Metabolomics and Lipidomics Platforms

About Proteomics Metabolomics / Lipidomics Analytical Services & Charges Team Contact

Service Charges (Excluding GST)

S. No

Code

Analytical Service Description

Academia & Start-Ups

Non-Academia

1

101

Protein extraction from tissues and cells

₹ 3,000 / sample

₹ 4,500 / sample

2

110

Desalting of protein lysate [compulsory for Synapt G2 (Q/TOF) mass spectrometer]

₹ 2,500 / sample

₹ 3,750 / sample

3

111

Protein estimation (BCA or Bradford assay)

₹ 1,000 / sample

₹ 1,500 / sample

4

301

Peptide purification using Zip-tips or Pierce C18 Spin Columns [compulsory for Orbitrap Eclipse mass spectrometer]

₹ 2,500 / sample

₹ 3,750 / sample

5

401

Metabolite/lipid extraction from tissue/cell/biofluids

₹ 2,500 / sample

₹ 3,750 / sample

6

601

Individual sample profiling or PRIDE files generation

₹ 1,500 / hour

₹ 2,250 / hour

7

602

Data processing using Progenesis-QI / Proteome Discoverer / Compound Discoverer software

₹ 1,000 / hour

₹ 1,500 / hour

A

Synapt G2 HDMS Q-TOF connected with nanoAquity UPLC system (Waters)

8

102

60 min nanoLC-MS/MS run #
(For those who require .RAW files only)

₹ 2,000 / sample

₹ 3,000 / sample

9

103

90 min nanoLC-MS/MS run #
(For those who require .RAW files only)

₹ 3,500 / sample

₹ 5,250 / sample

10

104

Protein Profiling of digested sample s#
(90 min nanoLC-MS/MS run +Database search)

₹ 5,000 / sample

₹ 7,500 / sample

11

105

Identification of proteins from coomassie stained gel band or gel spot
(60 min nanoLC-MS/MS run + Protein digestion + Database search)

₹ 5,000 / sample

₹ 7,500 / sample

12

106

Protein Profiling for less complex protein mixtures (i.e., purified protein) (60 min nanoLC-MS/MS run + Protein digestion + Database search)

₹ 5,000 / sample

₹ 7,500 / sample

13

107

Complex proteome analysis (discovery)
(90 min nanoLC-MS/MS run + Protein digestion + Database search)

₹ 6,500 / sample

₹ 9,750 / sample

14

108

Relative protein quantification by Label-free method
(90 min nanoLC-MS/MS run + Protein digestion + Database search + Quantitation)

₹ 7,000 / sample

₹ 10,500 / sample

15

109

For additional Technical replicate (additional nanoLC-MS/MS run)
(By default, each sample will be injected only 2 times)

₹ 1,500 / injection

₹ 2,250 / injection

B

UltraFlextreme MALDI-TOF/TOF (Bruker Daltonics)

16

201

Molecular weight (accurate mass) determination.

₹ 2,500 / sample

₹ 3,750 / sample

17

202

Polymer analysis

₹ 2,500 / sample

₹ 3,750 / sample

18

203

Oligo-nucleotides molecular weight determination

₹ 2,500 / sample

₹ 3,750 / sample

C

Orbitrap Eclipse Fusion Tribrid MS connected with UltiMate 3000 RSLCnano UHPLC system (Thermo Scientific)

19

302

60 min nanoLC-MS/MS run#
(For those who require .RAW files only)

₹ 4,000 / sample

₹ 6,000 / sample

20

303

140 min nanoLC-MS/MS run#
(For those who require .RAW files only)

₹ 6,000 / sample

₹ 9,000 / sample

21

304

Deep proteomics profiling of digested samples#
(140 min nanoLC-MS/MS run + Database search)

₹ 7,500 / sample

₹ 11,250 / sample

22

305

Identification of proteins from coomassie blue stained gels or immunoprecipitates
(60 min nanoLC-MS/MS run + Protein digestion + Peptide purification + Database search)*

₹ 9,000 / sample

₹ 13,500 / sample

23

306

Deep proteomics profiling (>2,500) from tissue/cell/biofluids.

(140 min nanoLC-MS/MS run + Protein digestion + Peptide purification + Database search)*

₹ 11,000 / sample

₹ 16,500 / sample

24

307

Labeled Quantification (TMT / iTRAQ) (After Fractionation- total 5 fractions) (300 min nanoLC-MS/MS run + Sample Preparation + Database search)
Note: Labeling will be done by the customer.

₹ 16,000

₹ 24,000

25

308

Identification of phosphorylation and other post-translational modification using ETD fragmentation
(60 min nanoLC-MS/MS run + Protein digestion + Peptide purification + Database search)*

₹ 9,000 / sample

₹ 13,500 / sample

26

309

Identification of phosphorylation and other post-translational modification using ETD fragmentation (140 min nanoLC-MS/MS run + Protein digestion + Peptide purification + Database search)*

₹ 11,000 / sample

₹ 16,500 / sample

27

310

Profiling of phosphorylated proteins following phospho-enrichment (TiO2 + Fe-NTA Magnetic Phosphopeptide Enrichment Kit)

₹ 14,000 / sample

₹ 21,000 / sample

28

311

For additional Technical replicate

(For proteomics, by default, each sample will be injected only 2 times)

₹ 4,000 / injection

₹ 6,000 / injection

29

312

For relative protein quantification in Orbitrap other than by TMT/ iTRAQ

[- additional charges will be applicable.]

₹ 750 / sample

₹ 1,125 / sample

D

Orbitrap Eclipse Fusion Tribrid MS connected with vanquish UHPLC system (Thermo Scientific)

30

402

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules
(Combined positive & negative modes in a single UPLC-MS/MS run + data analysis)

₹ 4,000 / sample

₹ 6,000 / sample

31

403

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules
(Positive & negative modes separately + data analysis separately for each mode)

₹ 6,000 / sample

₹ 9,000 / sample

32

404

UPLC-MS/MS for Untargeted analysis of Lipids/Small molecules
Single mode (positive or negative mode) identification + data analysis)

₹ 3,000 / sample

₹ 4,500 / sample

33

405

For additional Technical replicate

(By default, each sample will be injected only once for Untargeted analysis)

₹ 2,500 / injection

₹ 3,750 / injection

E

Altis Plus Triple Quadrupole MS connected with vanquish UHPLC system (Thermo Scientific)

34

501

ESI-MS based analysis mass/MW. Determination (without UPLC )*

₹ 1,500 / sample

₹ 2,250 / sample

35

502

UPLC-MS/MS for Targeted analysis of Lipids/Small molecules (Standards should be procured by the investigator)

5,000/sample + 300 per precursor ion

7,500/sample + 450 per precursor ion

36

503

UPLC-MS/MS for Targeted analysis of Lipids / Small molecules (already present in our panel)

2,500/sample + 300 per precursor ion

3,750/sample + 450 per precursor ion

37

504

Additional Technical replicate (By default, each sample will be injected only once for Targeted analysis of Lipids/Small molecules)

₹ 1,500 / injection

₹ 2,250 / injection

Notes

18% GST Extra as applicable

* Code no.501 would be done only for pure compound.

# Code no.102, 103, 104, AND 302, 303,304would be done only for purified peptides (after doing ZipTip or spin columns).

Contact

Rajiv Gandhi Centre for Biotechnology (RGCB),
Thycaud Post, Poojappura,
Thiruvananthapuram - 695 014, Kerala, India
+91-471-2529400 | 2347975 | 2348753
webmaster@rgcb.res.in

My Gov www.mygov.in Prime Ministers National Relife Fund India Gov www.india.gov.in Make In India www.makeinindia.gov.in Make In India www.iredibleindia.org Data gov in www.data.gov.in www.pib.gov.in

Last Updated on: June 21, 2024
CERT-In Certified Website