The Recombinant Protein Production, Purification, and Crystallization Facility at BRIC-RGCB in the Akkulam campus houses all essential equipment needed for the production and chromatographic purification of recombinant proteins, plus their crystallization. The facility has equipment such as a biosafety cabinet, 6-liter capacity shaker incubator, high-speed floor-type centrifuge, probe-type sonicator, French press, protein purification systems, crystallization robot, crystallization incubator, and a stereomicroscope with polarizer, along with several other smaller equipment and accessories.
Location
BRIC-RGCB Campus II, Aakkulam
|
Sl. No. |
Equipment |
Make / Model |
|
1 |
Class II, Type A2 Biosafety cabinet, 4 x 2 feet |
Biolinx / Biosafe 1200A2 |
|
2 |
Petri-plate incubator, 18 L capacity |
Thermo / IMC18 |
|
3 |
Refrigerated high-capacity stackable shaker incubator, 6 L capacity |
Eppendorf New Brunswick / Innova 44R |
|
4 |
Cuvette-type spectrophotometer |
Eppendorf / BioPhotometer D30 |
|
5 |
High speed, high-capacity floor-type centrifuge, 4 L capacity |
Thermo / Lynx 4000 |
|
6 |
Homogenizer with Teflon pestle |
Remi / RQT 127 A/D |
|
7 |
Probe-type Sonicator with sound-abating chamber |
Sonics / Vibra Cell VCX-750A |
|
8 |
Multi-cycle cell disruptor / French Press |
Constant / Multicycle 40KSI |
|
9 |
Liquid Nitrogen container 26 L capacity |
Indian Oil / Cryocan TA-26 |
|
10 |
Protein purification (FPLC) system-1 (in cold cabinet) |
Cytiva / AKTA Pure 25M |
|
11 |
Protein purification (FPLC) system-2 (at room temperature) |
Bio-Rad / NGC Quest 10 |
|
12 |
Microvolume UV-Vis spectrophotometer |
Thermo / Nanodrop One C |
|
13 |
Crystallization robot |
SPT Labtech / Mosquito Xtal3 |
|
14 |
Electronic Multichannel Pipette (10-200 μl) |
Brand / Transferpette-8 |
|
15 |
Dry Shipper with shipping case |
ICBiomedical / CX100 |
|
16 |
Crystallization-cooled incubator, 400 L capacity |
PHCbi / MIR 554 |
|
17 |
Trinocular stereomicroscope with polarizer set and camera |
Molecular Dimensions / MD5-915 |
