About us
The RGCB Genomics Sanger Sequencing Facility provides one-stop, high-precision DNA sequencing and genotyping solutions for academia, clinics, and industry in South India. Our centralized core leverages state-of-the-art 3730xl DNA Analyzers (48 & 96-capillary systems) for fast, robust, and reliable services tailored to diverse research needs, processing up to 96 samples in a two-hour run and completing as many as 12 plates daily. We support a broad spectrum of templates including purified PCR products and Fragment analysis genotyping with seamless sample submission and rapid digital delivery of results to customers nationwide. As of now, Sanger sequencing services only for good quality PCR products (QC results mandatory - concentration and purity) and ready to run (electrophoresis only)
What Sets Us Apart
- Expert Technology
Automated fluorescence capillary Sanger sequencing represents a cornerstone technology in molecular biology, renowned for its unparalleled accuracy and reliability with read lengths up to 800 bp (routine) and up to 1400 bp (optimized protocols), enabling targeted genotyping. This "gold-standard" method leverages the principles of dideoxy chain termination, coupled with the precision of capillary electrophoresis and the sensitivity of fluorescence detection, to deliver highly accurate DNA sequence information. Fluorescently labeled dideoxynucleotides are incorporated during DNA synthesis, and the resulting fragments are separated by size through fine capillaries. A laser excites the fluorescent tags, and detectors capture the emitted light, translating it into a chromatogram that visually represents the DNA sequence. Specialized software then interprets these signals, providing a highly accurate sequence output. This comprehensive approach ensures that researchers and clinicians can rely on the data generated for critical decisions in research, diagnostics, and therapeutics.
- Professional Support & Speed
Experience streamlined ordering, reliable overnight purified PCR product-to-sequence data workflow (Monday through Friday), and consultation from scientific staff with expertise in troubleshooting complex samples, primer design, and data validation.
- Training Services
Learn best practices for preparing your purified PCR products for optimal sequencing results. We offer hands-on training sessions for interested researchers. To apply, please use the web portal weblink.
Please note that separate charges apply for both training and sample processing. Customers are responsible for the quality control of their purified PCR product inputs, and sequencing charges are applicable regardless of the success or failure of the sequencing results. This combined training and sequencing service for purified PCR products is available only upon special request and after detailed discussion with our scientific staff.
- Results Delivery
Receive raw sequence files (ASCII), quality scored data, and chromatograms via email compatible with leading analysis software and downstream applications.
AI-enabled Bioinformatics Analysis (Optional Add-On)
Comprehensive bioinformatics support integrated with AI tools is available at extra cost. Services include sequence quality assessment, alignment, variant analysis, data visualization (e.g., plots/graphs), and custom analyses for publication or clinical reporting. Pricing depends on sample number and analysis complexity, with free consultation provided prior to project initiation. By integrating high-throughput sequencing with dedicated client service and advanced bioinformatics, the RGCB Genomics Sanger Sequencing Facility empowers researchers to unlock genetic insights with speed, accuracy, and confidence.
