Building
Sophisticated Trans-membrane Pores for Nanotechnology and Medicine
- Overview
Brief Description: This invention involves the
creation of synthetic transmembrane peptide pores formed from
short α-helical peptides, specifically derived from
porinACj from Corynebacterium jeikeium. This technology
enables single-molecule sensing and has significant implications
for nanopore technology in biotech applications.
Development Stage: The patent for this
innovative technology has been granted in India (Indian Patent
No. 407575, dated September 26, 2022).
- Key Features
- Unique Architecture – The developed
pore is the first large synthetic transmembrane pore to be
constructed entirely from short synthetic α-helical
peptides, showcasing original design in protein nanopore
technology.
- Ion Selectivity – The peptide pore
demonstrates ion-selective properties, allowing it to conduct
ions effectively while being capable of binding blockers like
cyclic sugars.
- Functional Analysis – Comprehensive
investigations reveal the pore’s assembly pathway and
structural properties, offering insights into its subunit
composition and dynamics.
- Benefits:
- Economic Impact: This technology paves the
way for advanced sensing applications, potentially leading to
novel diagnostic tools and therapies, thus benefiting the biotech
industry economically.
- Social Impact: By enhancing the capability
for single-molecule sensing, this invention could improve disease
detection and characterization, contributing to better healthcare
outcomes.
- Environmental Impact: The use of synthetic
peptides for nanopore construction presents a more sustainable
and controlled approach compared to natural peptide utilization,
minimizing ecological impact.
- Applications:
- This invention enhances the field of nanopore technology by
providing a stable platform for applications such as DNA
sequencing and macromolecule characterization.
- The pore’s unique properties offer various applications,
including drug delivery systems and ion-selective sensors.
- Research Opportunities: Further exploration is encouraged to
harness this technology for diverse applications in biomedicine
and molecular research.
- Case Studies:
- Through high-resolution single-channel electrical recordings,
the RGCB has successfully defined the structural properties of
the synthetic pore, providing foundational data for future
applications in molecular sensing.
- The study explores the interaction of the pore with
cyclodextrins, elucidating the functional properties and blocking
mechanisms, vital for further advancements in drug delivery and
molecular interaction studies.
- IP Status:
- Intellectual Property: Indian Patent No.
407575, protecting the synthesis and application of transmembrane
peptide pores for various biotechnological uses.
- Support Offered:
- Licensing Options: Opportunities are
available for non-exclusive licensing this innovative technology,
inviting collaboration with biotech and pharmaceutical companies
for further development.
- Technical Support: RBCB will provide
scientific guidance and assistance in navigating the applications
of synthetic trans-membrane pores, enabling effective utilization
of the invention.
- Collaboration Opportunities: Potential
partnerships with academic and research institutions to explore
novel applications and enhance the understanding of this
technology through further research.
- Contact Information:
Contact Person: Dr. K. R. Mahendran, Scientist, BRIC-RGCB
Email Address: mahendran@rgcb.res.in,techventures@rgcb.res.in
Phone Number: +91-471-2764-033