Building Sophisticated Trans-membrane Pores for Nanotechnology and Medicine
- Overview
Brief Description:This invention involves the creation of synthetic transmembrane peptide pores formed from short α-helical peptides, specifically derived from porinACj from Corynebacterium jeikeium. This technology enables single-molecule sensing and has significant implications for nanopore technology in biotech applications.
Development Stage: The patent for this innovative technology has been granted in India (Indian Patent No. 407575, dated September 26, 2022).
- Key Features
- Unique Architecture – The developed pore is the first large synthetic transmembrane pore to be constructed entirely from short synthetic α-helical peptides, showcasing original design in protein nanopore technology.
- Ion Selectivity – The peptide pore demonstrates ion-selective properties, allowing it to conduct ions effectively while being capable of binding blockers like cyclic sugars.
- Functional Analysis – Comprehensive investigations reveal the pore’s assembly pathway and structural properties, offering insights into its subunit composition and dynamics.
- Benefits:
- Economic Impact: This technology paves the way for advanced sensing applications, potentially leading to novel diagnostic tools and therapies, thus benefiting the biotech industry economically.
- Social Impact: By enhancing the capability for single-molecule sensing, this invention could improve disease detection and characterization, contributing to better healthcare outcomes.
- Environmental Impact: The use of synthetic peptides for nanopore construction presents a more sustainable and controlled approach compared to natural peptide utilization, minimizing ecological impact.
- Applications:
- This invention enhances the field of nanopore technology by providing a stable platform for applications such as DNA sequencing and macromolecule characterization.
- The pore’s unique properties offer various applications, including drug delivery systems and ion-selective sensors.
- Research Opportunities: Further exploration is encouraged to harness this technology for diverse applications in biomedicine and molecular research.
- Case Studies:
- Through high-resolution single-channel electrical recordings, the RGCB has successfully defined the structural properties of the synthetic pore, providing foundational data for future applications in molecular sensing.
- The study explores the interaction of the pore with cyclodextrins, elucidating the functional properties and blocking mechanisms, vital for further advancements in drug delivery and molecular interaction studies.
- IP Status:
- Intellectual Property: Indian Patent No. 407575, protecting the synthesis and application of transmembrane peptide pores for various biotechnological uses.
- Support Offered:
- Licensing Options: Opportunities are available for non-exclusive licensing this innovative technology, inviting collaboration with biotech and pharmaceutical companies for further development.
- Technical Support: RBCB will provide scientific guidance and assistance in navigating the applications of synthetic trans-membrane pores, enabling effective utilization of the invention.
- Collaboration Opportunities: Potential partnerships with academic and research institutions to explore novel applications and enhance the understanding of this technology through further research.
- Contact Information:
Contact Person: Dr. K. R. Mahendran, Scientist, BRIC-RGCB
Email Address: mahendran@rgcb.res.in,techventures@rgcb.res.in
Phone Number: +91-471-2764-033