Annexin V-Based Bead System for Apoptotic Cell Detection and Depletion
- Overview
Brief Description: This technology provides a cost-effective, multiplatform-compatible bead-based system specifically designed for the detection and depletion of apoptotic cells, particularly by utilizing Annexin V fluorescent conjugates to identify apoptosis through the quantification of phosphatidylserine on apoptotic cell surfaces.
Development Stage: The technology is currently at a commercially available stage, having been tested and validated for practical applications in various biological studies and clinical products.
- Key Features
- Multiplatform Compatibility – The technology is adaptable for use with multiple platforms including fluorescent microscopy and flow cytometry, allowing researchers to choose the method that best suits their needs.
- Dual Functionality– This system not only detects apoptotic cells but also allows for their effective depletion, enhancing utility in various biological applications and cell-based therapies.
- Immunomagnetic Approach – Utilizes non-fluorescent Annexin V combined with biotin and magnetic beads for efficient dead cell elimination, which is a unique feature not commonly found in other apoptosis detection kits.
- Benefits
- Economic Impact: This technology offers cost savings through the efficient identification and removal of apoptotic cells, thereby reducing waste and increasing the effectiveness of research and clinical applications.
- Social Impact: It has the potential to enhance the safety and efficacy of cell-based therapies, positively impacting patient outcomes in medical treatments.
- Environmental Impact: By improving the efficiency of biological research processes, the technology may reduce the overall consumption of reagents and materials, leading to less environmental waste.
- Applications:
- Pharmaceuticals– It can be utilized in drug discovery and cytotoxicity testing, allowing for more accurate assessments of drug effects on cell viability.
- Healthcare – The technology can be applied in clinical settings for the improvement of cell-based therapies, contributing to better management of diseases and personalized medicine.
- Research Opportunities: Further research could explore enhancements in the detection of apoptosis in different cell types or the development of new fluorescent dye conjugates for expanded analytical capabilities.
- Case Studies:
- A successful trial demonstrated the system’s effectiveness in identifying and depleting apoptotic cells from hematopoietic stem cell populations, leading to improved yields in therapy applications.
- In cancer research, this technology provided significant insights into the cellular effects of chemotherapeutic agents by allowing researchers to simultaneously evaluate apoptosis and eliminate dead cells from analyses.
- IP Status:
- Intellectual Property: The technology has a filed patent in India, Application No: 2020541102567, dated April 15th, 2020, with potential for extension to other jurisdictions.
- Support Offered:
- Licensing Options: A Non- exclusive licensing option is available for interested parties.
- Technical Support: Comprehensive technical support includes training resources and guidance on the use of the technology for optimal outcomes.
- Collaboration Opportunities: Opportunities exist for partnerships and joint development projects focusing on advanced applications of the technology.
- Contact Information:
Contact Person: Dr. T. R. Santhosh Kumar, Scientist, BRIC-RGCB
Email Address: trsanthosh@rgcb.res.in , techventures@rgcb.res.in
Phone Number: +91-471-2764-040