Mitochondria, having arisen from ancient alphaproteobacteria, are suggested to have created the immense evolutionary pressure for the elaboration of an endomembrane system in the earliest eukaryotes. It is intriguing that mitochondria-derived vesicles (MDVs), discovered a decade back, might be one of the first kind of intracellular vesicles encountered in ancestral eukaryotes/archaea. Therefore, there is likely to be a great extent of unexplored crosstalk between the endomembrane system and the mitochondria that keep mitochondria healthy. We will devise novel ways to purify these ~70 nm mitochondria derived vesicles from endothelial cells and uncover their roles in blood vessel formation.Preliminary observations suggest that RhoGTPases that play a role in vesicular traffickingare nonetheless important in mitochondrial function. I would like to expand on the potential role of vesicular RhoGTPases in mitochondrial health and their relevance to endothelial metabolism and stability of the vasculature.