The RGCB Genomics Sanger Sequencing Facility provides one-stop, high-precision DNA sequencing and genotyping solutions for academia, clinics, and industry in South India. Our centralized core leverages state-of-the-art 3730xl DNA Analyzers (48 & 96-capillary systems) for fast, robust, and reliable services tailored to diverse research needs, processing up to 96 samples in a two-hour run and completing as many as 12 plates daily. We support a broad spectrum of templates including PCR products, plasmids, cosmid and BAC clones, and gel-extracted fragments, with seamless sample submission and rapid digital delivery of results to customers nationwide.

What Sets Us Apart

1. Expert Technology
   Automated, fluorescence capillary Sanger sequencing ensures gold-standard accuracy, read lengths up to 800 bp (routine) and up to 1400 bp (optimized protocols), enabling targeted genotyping, SNP, SSR, AFLP, and fragment analysis. Quality control includes standard DNA runs in each plate for dependable, reproducible results.
2. Professional Support & Speed
   Experience streamlined ordering, reliable overnight sample-to-data workflow (Monday through Friday), and assistance from scientific staff with expertise in troubleshooting complex samples, primer design, and data validation.
3. Flexible Services
   We offer both routine and specialty workflows including difficult region sequencing, primer walking for extended reads, full plasmid sequencing, and microbial identification. Free consultations help assess project needs and select optimal protocols.
4. Results Delivery
   Receive raw sequence files (ASCII), quality scored data, and chromatograms via email compatible with leading analysis software and downstream applications.

Bioinformatics Analysis (Optional Add-On)

Comprehensive bioinformatics support is available at extra cost. Services include sequence quality assessment, alignment, variant analysis, data visualization (e.g., plots/graphs), and custom analyses for publication or clinical reporting. Pricing depends on sample number and analysis complexity, with free consultation provided prior to project initiation. By integrating high-throughput sequencing with dedicated client service and advanced bioinformatics, the RGCB Genomics Sanger Sequencing Facility empowers researchers to unlock genetic insights with speed, accuracy, and confidence.