The Mass Spectrometry and Proteomics Core Facility of RGCB aims to make cutting edge mass spectrometry technology available to RGCB researchers as well as the wider academic and life science industry community. Mass spectrometry for protein research helps in identification and quantification of biomolecules with increased sensitivity. Being a core facility, a major goal of this facility is to become a research environment for multidisciplinary research that utilizes mass spectrometry and other proteomics technologies to understand the disease biology and molecular medicine in the post-genomic era.

Infrastructure

RGCB Proteomics Core Facility houses the following instrumentation

Synapt G2-HDMS from Waters

This is a hybrid Quadrupole-Time of Flight (Q-TOF) mass spectrometer with an extra feature called Ion-Mobility by which compounds can be further separated and resolved based on their shape. The SYNAPT G2 system features new QuanTof technology that combines an innovative high field pusher and dual stage reflectron with a novel ion detection system in an optimised, folded, TOF geometry. Together these features provide a new dimension of high resolution, exact mass and quantitative performance uniquely available at acquisition rates that are compatible with UPLC separations.

UltrafleXtreme MALDI-TOF/TOF from Bruker Daltonik

This Mass spectrometer combines a MALDI source with a TOF/TOF tandem mass analyzer to acquire both high resolution peptide mass fingerprint data and MS/MS spectra of peptide ions. This MALDI-TOF/TOF Combines true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh performance and extreme flexibility for a broad variety of proteomics applications. In addition, the smartbeam-II™ laser enables ultra-high data acquisition speed in both MS and MS/MS at full systems performance