DBT-SAHAJ National Facility for Mass Spectrometry-Based Proteomics, Metabolomics and Lipidomics Platforms
The DBT-SAHAJ National Facility for Mass Spectrometry-based Proteomics, Metabolomics and Lipidomics platforms at RGCB aims to make cutting edge mass spectrometry technology available to RGCB researchers as well as the wider academic and life science industry community in the country. Mass spectrometry for biomedical research helps in identification and quantification of biomolecules with increased sensitivity. Being a core facility, major goal of this facility is to become a research environment for multidisciplinary research that utilizes mass spectrometry and other MS-based omics technologies to understand the disease biology and molecular medicine in the post-genomic era.
Infrastructure
DBT-SAHAJ National Facility for Mass Spectrometry houses the following instrumentation
UltraFlextreme MALDI-TOF/TOF (Bruker Daltonics)
This Mass spectrometer combines a MALDI source with a TOF/TOF tandem mass analyzer to acquire both high resolution peptide mass fingerprint data and MS/MS spectra of peptide ions. This MALDI-TOF/TOF Combines true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh performance and extreme flexibility for a broad variety of proteomics applications. In addition, the smartbeam-II™ laser enables ultra-high data acquisition speed in both MS and MS/MS at full systems performance
Synapt G2 HDMS Q-TOF connected with nanoAquity UPLC system (Waters)
This is a hybrid Quadrupole-Time of Flight (Q-TOF) mass spectrometer with an extra feature called Ion-Mobility by which compounds can be further separated and resolved based on their shape. The SYNAPT G2 system features new QuanTof technology that combines an innovative high field pusher and dual stage reflectron with a novel ion detection system in an optimised, folded, TOF geometry. Together these features provide a new dimension of high resolution, exact mass and quantitative performance uniquely available at acquisition rates that are compatible with UPLC separations.
Orbitrap Eclipse Tribrid MS connected with UltiMate 3000 RSLCnano UHPLC or vanquish UHPLC system (Thermo Fisher Scientific)
The Orbitrap Eclipse Tribrid mass spectrometer amplifies the power and versatility of the innovative Thermo Scientific™ Tribrid™ design by incorporating the latest inventions in ion transmission and control, extended m/z range (HMRⁿ) mode, optional Proton Transfer Charge Reduction (PTCR) and real-time decision making. These new functionalities provide ground-breaking gains in sensitivity, selectivity and versatility, making the instrument ideally suited for obtaining comprehensive results from proteomics, structural biology, small-molecule, and biopharmaceutical characterization experiments.
TSQ Altis Plus Triple Quadrupole MS connected with vanquish UHPLC system (Thermo Fisher Scientific)
With superior acquisition speeds, enhanced sensitivity, prototypical selectivity, and exceptional robustness; the TSQ Altis Plus mass spectrometer delivers unprecedented accuracy and precision for low-level compound detection and quantitation in complex matrices. Advanced control software, database integration, and templates streamline rigorous method development in TSQ Altis Plus for all types of targeted analysis.
Last Updated on: September 27, 2023
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