Pradeep Kumar G

Pradeep Kumar G, PhD

Scientist G
Reproductive Biology (2004 - 2022)

My group worked on membrane dynamics in mammalian spermatozoa during their post- testicular development and its relevance in making the spermatozoa undergo membrane fusion events associated with fertilization. Our studies demonstrated the presence of organized lipid-protein clusters in biomembranes allowing restricted intracluster molecular motion, while enjoying the freedom for lateral motion. These microdomains are widely known as membrane rafts today. We studied sperm membrane structure in the context of male infertility and evolved protocols to modulate the physical state of sperm membrane in vitro to aid in gamete interaction and subsequent fusion. We conducted differential display proteomics analysis to identify the differences in the proteins, both within and outside membrane rafts, in spermatozoa from fertile and infertile human males. This study led to the identification of several proteins which were essential for making the sperm fertilize and activate the oocyte missing in several males with primary male factor infertility. We conducted extensive studies on a select set of the critical molecules involved in making the sperm functionally competent to fertilize and activate the oocyte. We have made an antibody array for screening the expression of 54 differentially displayed human sperm proteins associated with male factor infertility, which could be used for diagnosing male factor infertility in terms of molecular markers and for optimizing assisted reproductive technology interventions. My group was also successful in spermatogonial stem cell reprogramming to transdifferentiate them into somatic lineages. We studied spermatogenesis using a network biology approach integrating the changes in the global epigenetic, transcriptional, post- transcriptional and proteomic landscapes using the 1st wave of spermatogenesis model in mouse to address the molecular networks and pathways involved in germline stem cell maintenance, division and differentiation.
I have completed 26 extramural research grants and have successfully mentored 23 PhDs. I have trained 7 post-doctoral fellows and several Master-level students. I have published 121 research articles and am a reviewer of many international journals, all the Indian funding agencies and several international funding agencies.

ALUMNI

Post-doctoral Fellows

Manish Nivsarkar, PhD (1994) [Devi Ahilya University]
Manish Nivsarkar, PhD (1994) [Devi Ahilya University]

Worked on the effect of secondary plant metabolites on sperm motility
Current Position:
Director, PERD Centre, Ahmedabad (2013-present)

Sunita Agnihotri, PhD (1998-2000 and 2002-2006)  [Devi Ahilya University]
Sunita Agnihotri, PhD (1998-2000 and 2002-2006) [Devi Ahilya University]

Identified and characterized NAD(P)H-dependent speroxide production in spermatozoa. Mapped the distribution and reorganizations in the distribution of this protein on the spermatozoa in relation to epididymal maturation, capacitation and acrosome reaction. Cloned and partially sequenced testis-expressed NOX-5.
Current Position:
Visiting Scientist at Centre for Advanced Technology, Indore, Madhya Pradesh

Anugya Mehta,  PhD (2006)
Anugya Mehta, PhD (2006)

Post-doctoral assignment involved the characterization of Erk1/2 activation and downstream signaling pathways during sperm development.
Current Position:
Scientist at Sree Chitra Tirunal Institute for Medical Science and Technology (SCTIMST), Thiruvananthapuram (2008-present)

C. Jasmin,  PhD (2008-2009)
C. Jasmin, PhD (2008-2009)

Involved in the cloning and expression of full-length clp-2 and clp-3 from mouse and human testicular germ cells. Also involved in the cloning and expression of cyclin b2 and a few polyubiquitins.
Current Position:
DST Woman Scientist at National Institute of Oceanography Regional Centre, Kochi

Indu S,  PhD (2013-2015)
Indu S, PhD (2013-2015)

Evaluated the genome stability in testicular germ line stem cells in a mouse model.
Current Position:
Post-Doctoral Fellow at University of Toledo, Ohio

Susha Kuttiyil, PhD (2018-2021)
Susha Kuttiyil, PhD (2018-2021)

AS a DBT-RA, Susha worked on elucidating the transcriptional regulation of Cnnm1 expression in SPG cells in order to validate and further analyze the function of the protein.

Soumya A, PhD (2019-2021)
Soumya A, PhD (2019-2021)

As a CSIR-RA, Soumya attempted to identify the functional promoter region and cis-acting elements that regulates the expression of TDP 43 and its spatio-temporal expression in the testis.

PhDs

Aparna Singh,  PhD (1989-1993) [Devi Ahilya University]
Aparna Singh, PhD (1989-1993) [Devi Ahilya University]

Thesis Title: Role of free radicals and superoxide dismutase in the modulation of sperm membrane conformation
Current Position:
Deputy Registrar
Centre for Continuing Education
University of Petroleum and Energy Studies
Dehradun, Uttarakhand.

Sumita Sinha,  PhD (1990-1994) [Devi Ahilya University]
Sumita Sinha, PhD (1990-1994) [Devi Ahilya University]

Thesis Title: Biochemical and biophysical studies of human spermatozoa with special reference to oligospermia
Current Position:
Research Scientist at Massachusetts General Hospital from 2003.

Suvro Chatterjee,  PhD (1994-1999) [Devi Ahilya University]
Suvro Chatterjee, PhD (1994-1999) [Devi Ahilya University]

Thesis Title: Biophysical and biochemical perturbations in spermatozoa during infertility induction in males
Current Position:
Scientist, Anna University-KBC Research Centre (2004-present). Leading a group working on vascular biology.

Prem Prakash Singh,  PhD (1990-1995) [Devi Ahilya University]
Prem Prakash Singh, PhD (1990-1995) [Devi Ahilya University]

Thesis Title: Iodine activation, peripheral conversion of thyroxine to triiodothyronine and biological alterations in thyroid follicular membranes with special reference to temperature in Columba livia
Current Position:
Assistant Professor at Govt. College, Balrampur, Chhattisgarh

Sharad Purohit, PhD (1994-2000) [Devi Ahilya University]
Sharad Purohit, PhD (1994-2000) [Devi Ahilya University]

Thesis Title: Distribution of surface saccharides and egg binding proteins on the surface of sperm cells of normal and oligospermic human subjects
Current Position:
Assistant Professor at Georgia Regents University, Augusta (2012-present)

Deeksha Saxena, PhD (1997-2002) [Devi Ahilya University]
Deeksha Saxena, PhD (1997-2002) [Devi Ahilya University]

Thesis Title: Characterization of implantation specific proteins and protein encoding genes in the mouse uterus
Current Position:
Research Scientist at University of Pennsylvania (2013-present)

Kumar Chandrakuntal , PhD [Devi Ahilya University]
Kumar Chandrakuntal , PhD [Devi Ahilya University]

Thesis Title: Blue light signal transduction in plant coleoptile tips: involvement of active oxygen species in phototropism
Current Position:
Post-doctoral fellow at Memorial Health University Medical Center, Savannah, Georgia (2011-present)

Mopidevi Brahmaraju, PhD(2002-2007) [Devi Ahilya University]
Mopidevi Brahmaraju, PhD(2002-2007) [Devi Ahilya University]

Thesis Title: Evaluation of the role of testis expressed proteins with reference to sperm development and fertilization
Current Position:
Assistant Professor, Dept. of Pathology, New York Medical College, Valhalla, NY

Mohammed Shoeb,  PhD (2002-2010)
Mohammed Shoeb, PhD (2002-2010)

Thesis Title: Identification and characterization of membrane lipid rafts in mammalian spermatozoa
Current Position:
Govt. LCS College, Ambagarh Chowki, Rajnandgaon, Chattisgadh

Ambika Chandrasekhar,  PhD (2004-2011)
Ambika Chandrasekhar, PhD (2004-2011)

Thesis Title: Molecular and functional characterization of testis expressed Nox5 in relation to sperm development
Current Position:
Post-doctoral Fellow in University of Alberta (2014-present) with special focus on diabetic neuropathy

Uma Chandran,  PhD(2005-2012)
Uma Chandran, PhD(2005-2012)

Thesis Title: Identification of testis expressed cell cycle regulating molecules with special reference to meiosis
Current Position:
Post doctoral fellow at Interdisciplinary School of Health Sciences at Savitribai Phule Pune University

Indu S,  PhD (2005-2012)
Indu S, PhD (2005-2012)

Thesis Title: Identification and functional evaluation of factors regulating testicular germ line stem cell division and differentiation
Current Position:
Post Doctoral Fellow at Dept. of Pathology
New York Medical College, Valhalla, NY

Sreesha Sree,  PhD (2008-2013)
Sreesha Sree, PhD (2008-2013)

Thesis Title: Role of MicroRNA in male germ cell differentiation
Current Position:
Post Doctoral Researcher, University of Helsinki

Divya Saro Varghese,  PhD (2008-2013)
Divya Saro Varghese, PhD (2008-2013)

Thesis Title: Functional Analysis of TDP-43 in Regulation to Spermatogenesis.
Current Position:
Researcher at United Arab Emirates University, College of Medicine and Health SCiences, Department of Biochemistry, Al Ain, Abu Dhabi , United Arab Emirates.

Bhagya K.P ,  PhD(2008-2013)
Bhagya K.P , PhD(2008-2013)

Thesis Title: Functional Analysis of AIRE (Autoimmune in Regulator) Germ Cell Development.

Karthika Radhakrishnan ,  PhD(2010-2017)
Karthika Radhakrishnan , PhD(2010-2017)

Thesis Title: H3K4 methylation and Spermatogenesis.
Current Position:
Post-Doctoral Researcher
Hudson Institute of Medical Research, Clayton, Victoria, Australia

Anil Kumar T.R,  PhD (2010-2017)
Anil Kumar T.R, PhD (2010-2017)

Thesis Title: Evaluation of Protocadherin Expression in Relation to Human Male Factor Infertility.
Current Position:
Guest Faculty, The Inter-University Centre for Evolutionary and Integrative Biology (iCEIB)
University of Kerala

Devi A.N, PhD (2013-2019)
Devi A.N, PhD (2013-2019)

Thesis Title: Expression Profiling and Functional Characterization of Nephrocystin in relation to Spermatogenesis.
Current Position:
Post-Doctoral Researcher, University of Calgary

Soumya A, PhD (2013-2019)
Soumya A, PhD (2013-2019)

Thesis Title: Molecular Evaluation of Interactions between Sperm Membrane Rafts and ZonaPellucida Proteins.

NomeshYadu, PhD (2009-2017)
NomeshYadu, PhD (2009-2017)

Thesis Title: Profiling of mouse testicular proteome in relation to germ cell development vin first wave of spermatogenesis and busulfan-induced germ cell deplection models.

Mahitha Sahadevan, PhD (2022)
Mahitha Sahadevan, PhD (2022)

Thesis Title:MicroRNA-mediated regulation of gene expression in the division and differentiation of spermatogonial cells in mouse testis
Current Position:
ICMR-SRF in the Molecular Reproduction Division at RGCB.

Irfan Khan, PhD (2023)
Irfan Khan, PhD (2023)

Thesis Title: Deciphering the role of CNNM1 in male germ cell establishment, maintenance and differentiation.

Jeeva S E, PhD (2024)
Jeeva S E, PhD (2024)

Thesis Title: Evaluation of the role of DYNLT1 in stemness, spermatogenesis and infertility