Bio-imaging Facility

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In Vivo Small Animal Imager (IVIS Spectrum, Xenogen)

IVIS Spectrum, the small animal imager from Xenogen, Perkin Elmer, USA is an advanced animal imager that can support both fluorescence and bioluminescence imaging with high sensitivity. This equipment supports both Trans and epi illumination enabling 3D diffuse fluorescence tomography for depth perception. Also equipped with small animal anesthesia unit.

Flow Cytometer Sorters

High Speed Flowcytometer Sorter System: (FACSAria III)

FACSAria III is a Bench top fixed aligned high speed 4 way sorter system from Becton Dickinson, USA and is equipped with the laser lines, 488 nm, 355 UV, 405 Violet lasers and 633 nm. The machine allows plate sorting. Also equipped with aerosol management system.

High Speed Flowcytometer Sorter System: (FACSAria II)

FACS Aria II is a Bench top fixed aligned high speed 4 way sorter systems from Becton Dickinson, USA and is equipped with the laser lines 488 nm,375 UV, 405 Violet lasers, and 633 nm. Also equipped with aerosol management system.

High Speed Flowcytometer Sorter System: (FACSAria III)

FACS Aria III, Bench top fixed aligned flow cytometer is a high speed 4 way sorter system from Becton Dickinson, USA and is equipped with the following laser lines 488 nm Laser, 375 nm, 405 nm Violet lasers, 561nm laser and 633 nm laser.

Beckman Coulter Astrios EQ High Speed Cell Sorter

Six-way jet-in-air sorter with 7 lasers (355nm, 405nm, 488nm,532nm, 561nm, 592nm,640nm) and 22 fluorescent color capability. It has dual forward scatter PMTs that allow researchers to analyze biological samples ranging in size from 0.2um to 30um. The instrument is capable of simultaneous 1-6 way sorting and is equipped with Cyclone sorting for single cell sorting. Available nozzle sizes are 70um and 100um. It is contained within a Baker SterilGARD Class II biological safety cabinet for optimised sterile sorting.

Location: Akkulam Campus, Central Facility

Contact

Tilak Prasad
tilakprasad@rgcb.res.in
+91-9995704243

Confocal Microscopy

Spectral Confocal Microscope with Resonance Scanner for Fast Live Cell Imaging (A1Rsi)

A1R si, NIKON is the most advanced and fully automated spectral confocal microscope from Nikon, capable of capturing high quality confocal images with high speed and sensitivity. This machine includes a 32 array spectral detector and high speed resonance scanner that can achieve more than 25 frames per second at 512x512 pixel dimension apart from motorized xy stage for multipoint confocal imaging. Available laser lines are Multi-line Ar laser with 458/488/514nm, Diode laser 561 nm, HeNe 633 nm, Blue diode Laser 405nm, all with AOTF control. Also supported by live cell incubation chamber from Okolab for live cell imaging.

Confocal Laser scanning Microscope with high sensitivity spectral detector (Olympus FV3000)

Olympus FV3000 is equipped with a high sensitivity spectral detector (HSD) with GsAsP PMTs which enables it to view samples having weak emission. The desired emission range can be selected using the spectral detectors. The Diode Laser lines available are 405nm, 488nm, 514nm, 561nm and 640nm with 4x, 10x, 20x, 40x and 60x objectives.

Super Resolution Microscope for Live Cell Imaging and Confocal Attachment ( N-SIM, A1R)

N-SIM, A1R is a Structured Illumination Based Super-Resolution Microscope from Nikon, Japan with all accessories for live cell imaging that can achieve an X-Y resolution of 100nm and Z resolution of 320nm. The system is equipped with laser lines 405nm, 488nm, 561nm and 640 nm .

Confocal Laser Scanning Microscope with GaAsP Detector for Multifluorescence and Live Cell Imaging ( Leica SP8 Wll Confocal Microscope)

Leica SP8 Spectral Confocal with WLL is an advanced confocal microscope from Leica Microsystems, Germany. This equipment is configured with white light laser (WLL) that can support any laser lines between 470-670nm and AOBS for filter less emission tuning, in addition to the highly sensitive GaAsP detector.

Confocal Laser Scanning Microscope with Spectral Detector for Multifluorescence and Live Cell Imaging ( Leica SP2 Confocal Microscope)

Leica SP2 Laser scanning Spectral Confocal system is an advanced confocal microscope from Leica Microsystems, Germany. This equipment is configured with AOBS as tunable dichroic for all laser lines Argon ( 458, 477, 488,515nm) , 561 nm Diode laser and HeNe 633 nm laser lines. This confocal machine is also equipped with temperature, humidity and Co2 control system to enable imaging of both live and fixed samples.

Multiphoton Confocal Imaging system: Nikon A1R MP

The multiphoton confocal imaging system A1R MP is the dual line IR laser integrated multiphoton confocal with all required accessories and imaging softwares from Nikon, Japan. The system is capable of simultaneous or sequential image collection using galvanometer and resonant scanners and four photomultiplier fluorescence detectors and a DIC transmission detector. The multiphoton component is equipped with the following laser excitation source detectors in addition to the conventional visible detectors.

  1. Coherent Chameleon II Dual Line Tunable laser for MP imaging with a tuning range of 660nm to 1320nm as output A and 1040nm as output B.
  2. Galvanometer and Resonant scanners.
  3. Nikon Apo 25xW, CFI Apo 40xW NIR and CFI 60XW NIR objective for MP imaging.
  4. Four PMT detectors and four GaAsP NDD (reflected light) detectors.
  5. NIS Elements software controlling all microscope components.

Location: BIC, KINFRA CAMPUS

Contact

Ayswarya R.S
ayswaryars@rgcb.res.in

High Content Imaging System: Imagexpress Confocal HT.AI, Molecular device

The ImageXpress® Confocal HT.ai High-Content Imaging System is an advanced imaging platform with seven-channel laser light source and eight imaging channels to enable highly multiplexed assays while maintaining high throughput by using shortened exposure times. Designed for experiments involving live cells as the spinning disk reduces phototoxicity and enables fast acquisition. The system supports slides and one to 1536-well microplates, round or flat bottom, low to high profile. The main features of the system are

  1. Inverted microscope (ImageXpress Confocal HT.ai)
  2. Precision motorized X-Y (sample) stage
  3. Dual disk unit allowing selection between 3 modes
  4. 60 micron pinhole disk for basic confocal requirements
  5. 50 micron slit for high throughput confocal requirements
  6. Widefield (not-confocal) imaging mode
  7. 4 megaPixel sCMOS
  8. 8 position emission wheel and 5 position dichroic filter wheel
  9. Temp and air control system

Location: Akkulam Campus

Contact

Laiza Paul
laizapaul@rgcb.res.in

Spectral Karyotyping Platform: Metasystem -Zeiss AxioImager

The spectral karyotype platform from Metasystems, is an advanced imaging system for the automated detection of metaphases, FISH and spectral karyotyping. This unit equipped with fully motorised ZEISS Axioimager Z2 with Metafer, automated software with AI capabilities, with the interactive karyotyping software Ikaros and ISIS

Ikaros provides AI-driven chromosome separation and classification in metaphases of all common chromosome banding methods (e.g., G-Banding, R-Banding, and Q-Banding). Also, different tissue types, e.g., lymphocytes, bone marrow, amniotic fluid, and chorionic villi are supported. Options for color karyotyping, Multicolor FISH (mFISH), and Multicolor Chromosome Banding (mBAND) are also available.

Location: Akkulam Campus

Contact

Sanjai. D
sanjaid@rgcb.res.in

Seahorse XFe96 Analyzer

Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function. The equipment is compatible only with Seahorse XF test kits and other Seahorse XF reagents.

Location: Akkulam Campus

Contact

Sanjai. D
sanjaid@rgcb.res.in

Dynamic Light scattering (DLS) system

The Horiba nanoPartica SZ-100 V2 system is a Dynamic Light scattering instrument which is typically used for the measurement of the size distribution of particles emulsions, polymers and biomolecules dispersed or dissolved in a liquid and it can be also used to measure the Zeta potential of particles in solution

Location: Akkulam Campus

Contact

Harikrishnan K S
harikrishnanks@rgcb.res.in

Rodent Endoscopy System

Mouse colonoscope system: Coloview high-resolution mouse endoscopy system (KARL STORZ SE & Co. KG, Germany). This system can be used for colonoscopic examination and biopsy in mice and rats

Contact

Dr. Archana S
archanas@rgcb.res.in

Contact

Rajiv Gandhi Centre for Biotechnology (RGCB),
Thycaud Post, Poojappura,
Thiruvananthapuram - 695 014, Kerala, India
+91-471-2529400 | 2347975 | 2348753
webmaster@rgcb.res.in

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Last Updated on: June 21, 2024
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