Spermatogenesis in mammals is a complex process of germ cell differentiation that is regulated by unique control points. Identification of the key regulatory proteins would augment our understanding of the basic molecular mechanism of spermatogenesis and would serve as molecular tools for the diagnosis and treatment of spermatogenesis disorder. One such protein reported to be associated with human male factor infertility is Cyclin M1 (CNNM1). Using a mouse model, we demonstrated that Cnnm1 is expressed only in undifferentiated spermatogonial cells (SPG) which includes spermatogonial stem cells (SSCs) and/or A-type spermatogonial cells compared to the differentiated SPG cells which enter meiosis. Further, CNNM1 silencing studies showed that the protein is required for maintaining stemness and self-renewal in SPG cells. My study aims to understand the transcriptional regulation of Cnnm1 expression in SPG cells in order to validate and further analyze the function of the protein.
Identification and Characterization of the functional Promoter of TDP 43.
TDP 43 is an evolutionarily conserved, ubiquitously expressed DNA/RNA binding protein with diverse functions. The abnormal TDP 43 is discovered as a major causative link for several neurodegenerative disorders. Studies identified that the regulated expression of Acrv1 gene within the testes is through the repressive effect of TDP 43 by binding at the promoter region. Though TDP 43 is identified as essential for spermatogenesis and sperm formation, its expression is restricted in some cell types and stages. There is no direct evidence suggesting the regulation of gene expression at the transcription level of TDP 43. This study envisages to identify the functional promoter region and cis-acting elements that regulates the expression of TDP 43 thereby giving idea about its spatio-temporal expression in the testis.
MicroRNA-mediated regulation of gene expression in the division
and differentiation of spermatogonial cells in mouse testis.
Irfan Khan P
Role of CNNM1 in male germ cell development and differentiation.
Jeeva S E
Evaluation of the role of Dynlt1b in stem cell self renewal, stemness, spermatogenesis and infertility.
Inter-relationship between polymorphism in four obesity genes, their expression and its correlation with infertility & obesity in subjects from Kerala.
Transdifferentiation of spermatological stem cells (SSC) into somatic lineages via embryonic stem cell (ES)- like intermediaries.
Rajiv Gandhi Centre for Biotechnology (RGCB), Thycaud Post,
Poojappura, Thiruvananthapuram - 695 014, Kerala, India +91-471-2529400
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